Operophtera brumata reovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; unclassified Reoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q30C80|Q30C80_9REOV Uncharacterized protein OS=Operophtera brumata reovirus OX=352248 PE=4 SV=1
MM1 pKa = 7.42EE2 pKa = 5.65RR3 pKa = 11.84LINSYY8 pKa = 8.75RR9 pKa = 11.84TISHH13 pKa = 6.6SLRR16 pKa = 11.84QLVPAYY22 pKa = 7.18TTFEE26 pKa = 4.28DD27 pKa = 5.8LEE29 pKa = 4.45AQLPPDD35 pKa = 3.59YY36 pKa = 10.94VFPEE40 pKa = 4.25SFQYY44 pKa = 9.99PPRR47 pKa = 11.84IRR49 pKa = 11.84YY50 pKa = 9.18RR51 pKa = 11.84EE52 pKa = 3.87IHH54 pKa = 6.41SDD56 pKa = 2.94AGFTRR61 pKa = 11.84EE62 pKa = 4.25VEE64 pKa = 3.72AAMAGRR70 pKa = 11.84LANDD74 pKa = 3.46EE75 pKa = 4.98DD76 pKa = 4.15NPIMFDD82 pKa = 3.03YY83 pKa = 10.67RR84 pKa = 11.84EE85 pKa = 3.97PVRR88 pKa = 11.84PGRR91 pKa = 11.84IANWLTGYY99 pKa = 11.05AEE101 pKa = 4.3AANTLFDD108 pKa = 3.79QVLSEE113 pKa = 4.12VDD115 pKa = 3.07ALASVFYY122 pKa = 10.93DD123 pKa = 2.85VDD125 pKa = 3.33EE126 pKa = 4.28LLRR129 pKa = 11.84RR130 pKa = 11.84RR131 pKa = 11.84FQEE134 pKa = 3.92GDD136 pKa = 3.88VIPNDD141 pKa = 3.79HH142 pKa = 7.25DD143 pKa = 4.2HH144 pKa = 7.37PSNDD148 pKa = 2.97VLEE151 pKa = 4.71PSEE154 pKa = 4.85HH155 pKa = 5.6EE156 pKa = 3.99AARR159 pKa = 11.84VHH161 pKa = 6.53SDD163 pKa = 3.06QVVEE167 pKa = 4.24EE168 pKa = 4.29RR169 pKa = 11.84VQRR172 pKa = 11.84PLVRR176 pKa = 11.84LEE178 pKa = 4.18SPGEE182 pKa = 3.73DD183 pKa = 2.98EE184 pKa = 5.32IIFRR188 pKa = 11.84GPSDD192 pKa = 3.13IRR194 pKa = 11.84YY195 pKa = 7.72TPITLRR201 pKa = 11.84THH203 pKa = 6.24FHH205 pKa = 6.59FKK207 pKa = 9.5TFEE210 pKa = 4.04RR211 pKa = 11.84PVEE214 pKa = 4.02KK215 pKa = 10.45DD216 pKa = 2.95QPYY219 pKa = 10.65PSFKK223 pKa = 10.77VNQLHH228 pKa = 7.16PNTGLDD234 pKa = 3.51FSGRR238 pKa = 11.84PISSSFISAFNMSINPEE255 pKa = 4.15LASVTSALVYY265 pKa = 10.48LLNEE269 pKa = 3.48GRR271 pKa = 11.84YY272 pKa = 9.25GVRR275 pKa = 11.84DD276 pKa = 3.67DD277 pKa = 4.25SLVYY281 pKa = 10.41SHH283 pKa = 6.64NTTHH287 pKa = 6.12NQISPASTKK296 pKa = 10.5AALKK300 pKa = 9.27MEE302 pKa = 4.0EE303 pKa = 4.08RR304 pKa = 11.84VRR306 pKa = 11.84DD307 pKa = 3.67SVTYY311 pKa = 9.99FSSKK315 pKa = 10.16RR316 pKa = 11.84LTRR319 pKa = 11.84GQCEE323 pKa = 3.59HH324 pKa = 7.72LIRR327 pKa = 11.84HH328 pKa = 6.23MISYY332 pKa = 9.85HH333 pKa = 6.02INTPDD338 pKa = 3.01THH340 pKa = 7.81GLIVEE345 pKa = 4.84CYY347 pKa = 8.97EE348 pKa = 4.05QVGDD352 pKa = 4.07LRR354 pKa = 11.84ADD356 pKa = 3.62FTLEE360 pKa = 3.82SLRR363 pKa = 11.84IPLLNVDD370 pKa = 3.71WTRR373 pKa = 11.84ICYY376 pKa = 10.11NISNALEE383 pKa = 4.25NEE385 pKa = 4.11LEE387 pKa = 3.97RR388 pKa = 11.84AIFDD392 pKa = 3.25VLMFVAIMKK401 pKa = 9.21FSVVVTEE408 pKa = 3.96PQRR411 pKa = 11.84MVDD414 pKa = 3.55IAPGPFAVSAFLGCVPGSVVTHH436 pKa = 6.85DD437 pKa = 3.64AFKK440 pKa = 10.6TEE442 pKa = 3.86WKK444 pKa = 9.08RR445 pKa = 11.84TISRR449 pKa = 11.84PNSSTEE455 pKa = 3.48WPLTKK460 pKa = 9.97TSYY463 pKa = 10.54HH464 pKa = 6.1FPQAIGVPCTQVMPITRR481 pKa = 11.84RR482 pKa = 11.84PDD484 pKa = 3.23FQMFGCLLSMVINNMM499 pKa = 3.49

Molecular weight: 57.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q30C79|Q30C79_9REOV Uncharacterized protein OS=Operophtera brumata reovirus OX=352248 PE=4 SV=1
MM1 pKa = 7.63EE2 pKa = 5.49NDD4 pKa = 4.08SYY6 pKa = 11.78LSDD9 pKa = 3.68LTPFVGPVSYY19 pKa = 10.64VGRR22 pKa = 11.84NLTRR26 pKa = 11.84ILYY29 pKa = 9.38DD30 pKa = 3.76LQALSSKK37 pKa = 10.49CRR39 pKa = 11.84GKK41 pKa = 10.74VLLSSKK47 pKa = 9.17TGQPPRR53 pKa = 11.84VQVTRR58 pKa = 11.84ILVEE62 pKa = 3.86QMFTVYY68 pKa = 9.22TQLRR72 pKa = 11.84GYY74 pKa = 9.57NFSVTLTSLLNEE86 pKa = 4.76LSTALAMSVGRR97 pKa = 11.84SEE99 pKa = 4.33LTLNSNVLYY108 pKa = 9.98MITNVNSDD116 pKa = 2.96IAISIGKK123 pKa = 8.13VWNGVPPLRR132 pKa = 11.84LLNGSGSNADD142 pKa = 3.28VTIIGDD148 pKa = 3.93DD149 pKa = 3.97NILTRR154 pKa = 11.84MLRR157 pKa = 11.84KK158 pKa = 9.84LPGEE162 pKa = 4.0ARR164 pKa = 11.84VEE166 pKa = 4.07LTKK169 pKa = 10.64LAQVSGQLAGVRR181 pKa = 11.84VVRR184 pKa = 11.84QKK186 pKa = 11.45DD187 pKa = 3.68VTTLLADD194 pKa = 3.48QLFSRR199 pKa = 11.84SSGSIDD205 pKa = 3.39LARR208 pKa = 11.84IFVQMNKK215 pKa = 9.19QATVLSQFKK224 pKa = 9.97TDD226 pKa = 4.57FLMTPLSRR234 pKa = 11.84FHH236 pKa = 7.44DD237 pKa = 3.99LGRR240 pKa = 11.84PLFIEE245 pKa = 4.33IDD247 pKa = 3.87LVLTKK252 pKa = 10.48ADD254 pKa = 3.85SNSILSAPSISGEE267 pKa = 3.96VLFTIRR273 pKa = 11.84STADD277 pKa = 2.99VEE279 pKa = 4.51LSFSILRR286 pKa = 11.84GEE288 pKa = 4.06TSTIIKK294 pKa = 8.25LQPYY298 pKa = 9.13SHH300 pKa = 5.76VQRR303 pKa = 11.84FGEE306 pKa = 4.37KK307 pKa = 7.86VTFPTGKK314 pKa = 10.52GGILVTATGDD324 pKa = 3.28LRR326 pKa = 11.84NSVRR330 pKa = 11.84SGQRR334 pKa = 11.84SLIEE338 pKa = 3.83MGIEE342 pKa = 4.01GTLGQTTNTEE352 pKa = 3.94LSRR355 pKa = 11.84WFYY358 pKa = 11.03QIVTPILPYY367 pKa = 10.79LPGIVSMLTTSQPFNSVTRR386 pKa = 11.84VISPEE391 pKa = 3.51VSLYY395 pKa = 9.69FQNLKK400 pKa = 10.43PSFYY404 pKa = 10.76EE405 pKa = 4.17DD406 pKa = 3.98LLTTGTTPADD416 pKa = 3.32KK417 pKa = 10.35QRR419 pKa = 11.84RR420 pKa = 11.84VLVAYY425 pKa = 7.58TLILLIPEE433 pKa = 4.13YY434 pKa = 10.67LLNPVITLNNVNEE447 pKa = 4.51TPPFMSMYY455 pKa = 9.73GALVGHH461 pKa = 6.5FRR463 pKa = 11.84QIYY466 pKa = 7.59EE467 pKa = 3.89

Molecular weight: 51.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

7901

437

1358

790.1

89.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.404 ± 0.342

0.899 ± 0.174

5.594 ± 0.233

5.442 ± 0.256

4.949 ± 0.311

4.999 ± 0.186

2.493 ± 0.298

6.404 ± 0.264

3.721 ± 0.237

10.543 ± 0.523

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.569 ± 0.177

4.303 ± 0.171

4.772 ± 0.263

4.354 ± 0.283

6.683 ± 0.174

7.543 ± 0.422

7.771 ± 0.416

7.1 ± 0.25

0.987 ± 0.105

3.468 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski