Leptospira interrogans serovar Bataviae str. HAI135

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Leptospirales; Leptospiraceae; Leptospira; Leptospira interrogans; Leptospira interrogans serovar Bataviae

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6014 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M6TVE3|M6TVE3_LEPIR Uncharacterized protein OS=Leptospira interrogans serovar Bataviae str. HAI135 OX=1085538 GN=LEP1GSC170_0551 PE=4 SV=1
MM1 pKa = 7.55EE2 pKa = 4.98VGNDD6 pKa = 3.28QTNWCGWDD14 pKa = 3.25PTYY17 pKa = 11.35VGGGAFSTNPANWLRR32 pKa = 11.84FAGLRR37 pKa = 11.84YY38 pKa = 9.79VDD40 pKa = 4.23YY41 pKa = 11.27NQITNPMNSVSLFNIGGGDD60 pKa = 3.75GFDD63 pKa = 4.7LGDD66 pKa = 3.94ANFGNSGTGCNGALSMDD83 pKa = 3.9LQNNGFLYY91 pKa = 10.96VKK93 pKa = 10.41LSSAKK98 pKa = 10.34VILPALQIPGANEE111 pKa = 3.68NPDD114 pKa = 3.24IDD116 pKa = 4.7GVIAKK121 pKa = 9.35QLNN124 pKa = 3.43

Molecular weight: 13.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M6TD65|M6TD65_LEPIR Uncharacterized protein (Fragment) OS=Leptospira interrogans serovar Bataviae str. HAI135 OX=1085538 GN=LEP1GSC170_5760 PE=4 SV=1
MM1 pKa = 7.46NFRR4 pKa = 11.84FRR6 pKa = 11.84SFLNPIRR13 pKa = 11.84KK14 pKa = 9.16IIRR17 pKa = 11.84TISLHH22 pKa = 4.83QAGILVKK29 pKa = 10.73KK30 pKa = 10.13NFRR33 pKa = 11.84TFRR36 pKa = 11.84TRR38 pKa = 11.84KK39 pKa = 9.37LL40 pKa = 3.3

Molecular weight: 4.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6014

0

6014

1085766

30

1915

180.5

20.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.171 ± 0.04

0.91 ± 0.013

4.688 ± 0.026

7.046 ± 0.048

5.788 ± 0.04

6.363 ± 0.043

1.691 ± 0.015

8.0 ± 0.037

7.856 ± 0.039

10.26 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.091 ± 0.016

5.08 ± 0.034

3.822 ± 0.024

3.269 ± 0.022

4.625 ± 0.025

7.868 ± 0.04

5.024 ± 0.032

5.748 ± 0.034

1.139 ± 0.017

3.56 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski