Nitrospira sp. KM1

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; Nitrospiraceae; Nitrospira; unclassified Nitrospira

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4311 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A679HX64|A0A679HX64_9BACT Ribosome-binding factor A OS=Nitrospira sp. KM1 OX=1936990 GN=rbfA PE=3 SV=1
MM1 pKa = 7.59SGTPVAGGGVHH12 pKa = 5.93YY13 pKa = 9.24FANAFAVTPDD23 pKa = 3.05SSAIVYY29 pKa = 9.74LADD32 pKa = 3.44EE33 pKa = 4.46TTDD36 pKa = 3.69NVLEE40 pKa = 4.49TYY42 pKa = 10.03RR43 pKa = 11.84VPFTTPGSSTRR54 pKa = 11.84LNGPIVAGGSVSSLLGFSILPDD76 pKa = 3.26SSGIVYY82 pKa = 10.27AADD85 pKa = 3.45EE86 pKa = 4.4AVDD89 pKa = 5.04DD90 pKa = 4.63VIEE93 pKa = 4.67LYY95 pKa = 10.69RR96 pKa = 11.84SDD98 pKa = 3.83FSTPGVSTKK107 pKa = 10.66QNGPLVAGGNVDD119 pKa = 3.54GFIVQQ124 pKa = 3.75

Molecular weight: 12.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A679I432|A0A679I432_9BACT Uncharacterized protein OS=Nitrospira sp. KM1 OX=1936990 GN=W02_35850 PE=4 SV=1
MM1 pKa = 7.67FEE3 pKa = 4.35RR4 pKa = 11.84IIRR7 pKa = 11.84SVSGFVRR14 pKa = 11.84GLRR17 pKa = 11.84LTWHH21 pKa = 6.06IVKK24 pKa = 10.29GALITAIHH32 pKa = 6.36FRR34 pKa = 11.84HH35 pKa = 6.62ADD37 pKa = 3.42PNEE40 pKa = 4.16RR41 pKa = 11.84DD42 pKa = 3.5VMIQQWSRR50 pKa = 11.84ACLAILNVRR59 pKa = 11.84LRR61 pKa = 11.84IHH63 pKa = 6.93GDD65 pKa = 3.46VPSSAPAILFAANHH79 pKa = 5.64VSWLDD84 pKa = 3.37ILGLNAVKK92 pKa = 10.4RR93 pKa = 11.84VRR95 pKa = 11.84FVAKK99 pKa = 10.21SEE101 pKa = 3.87VRR103 pKa = 11.84RR104 pKa = 11.84WPLVGWLAKK113 pKa = 10.26QADD116 pKa = 3.89TLFIKK121 pKa = 10.53RR122 pKa = 11.84NSPSEE127 pKa = 4.29LIPINRR133 pKa = 11.84SLHH136 pKa = 5.39IALKK140 pKa = 9.94GGQSVGLFPEE150 pKa = 5.1GTTSSGRR157 pKa = 11.84SVLRR161 pKa = 11.84FHH163 pKa = 7.47SGLFEE168 pKa = 4.21SAVTSRR174 pKa = 11.84SAVCPVALSYY184 pKa = 8.94QTSDD188 pKa = 2.6GHH190 pKa = 5.96PCFRR194 pKa = 11.84AAFVGNQTLLASIWNVLTLPVLYY217 pKa = 10.64LNLHH221 pKa = 6.19FMPPLSSSQFDD232 pKa = 3.21RR233 pKa = 11.84HH234 pKa = 5.9EE235 pKa = 4.43LSQHH239 pKa = 5.19ARR241 pKa = 11.84TAILAKK247 pKa = 10.26VSHH250 pKa = 6.26TEE252 pKa = 4.23CPWSVAHH259 pKa = 6.92AAGSQVEE266 pKa = 4.54CAHH269 pKa = 6.63RR270 pKa = 4.09

Molecular weight: 29.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4311

0

4311

1283078

40

3133

297.6

32.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.511 ± 0.041

0.966 ± 0.014

5.295 ± 0.025

5.84 ± 0.037

3.729 ± 0.023

7.91 ± 0.043

2.403 ± 0.018

5.384 ± 0.027

3.984 ± 0.035

10.226 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.498 ± 0.018

2.904 ± 0.03

5.034 ± 0.031

3.895 ± 0.022

6.917 ± 0.038

6.176 ± 0.03

5.729 ± 0.038

7.621 ± 0.036

1.343 ± 0.017

2.634 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski