Phascolarctos cinereus (Koala)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Metatheria; Diprotodontia; Phascolarctidae; Phascolarctos

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37679 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P5KV37|A0A6P5KV37_PHACI transcription factor Sp8 isoform X1 OS=Phascolarctos cinereus OX=38626 GN=SP8 PE=4 SV=1
MM1 pKa = 8.24DD2 pKa = 4.75FQQLADD8 pKa = 5.0VADD11 pKa = 4.16KK12 pKa = 10.25WCSNTPFDD20 pKa = 6.16LIATEE25 pKa = 4.06EE26 pKa = 4.36TEE28 pKa = 4.33RR29 pKa = 11.84RR30 pKa = 11.84MDD32 pKa = 4.29FYY34 pKa = 11.47ADD36 pKa = 3.74PGVSFYY42 pKa = 10.98VLCPDD47 pKa = 4.64NGCGDD52 pKa = 3.96NFHH55 pKa = 6.3VWSEE59 pKa = 4.48SEE61 pKa = 3.97DD62 pKa = 3.63CLPFLQLAQDD72 pKa = 4.45YY73 pKa = 10.71ISSCGKK79 pKa = 8.56KK80 pKa = 7.94TLHH83 pKa = 6.31EE84 pKa = 4.11VLEE87 pKa = 4.47KK88 pKa = 10.61VFKK91 pKa = 10.63SFRR94 pKa = 11.84PLLGLPDD101 pKa = 3.71VDD103 pKa = 4.94DD104 pKa = 5.05DD105 pKa = 4.75AFEE108 pKa = 4.62EE109 pKa = 4.52YY110 pKa = 10.51SADD113 pKa = 3.68VEE115 pKa = 4.38EE116 pKa = 5.09EE117 pKa = 4.11EE118 pKa = 5.52PEE120 pKa = 4.6ADD122 pKa = 3.57HH123 pKa = 6.41QQMGVSQQQ131 pKa = 2.96

Molecular weight: 14.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P5JDU1|A0A6P5JDU1_PHACI doublesex- and mab-3-related transcription factor A1 OS=Phascolarctos cinereus OX=38626 GN=DMRTA1 PE=3 SV=1
AA1 pKa = 6.99QRR3 pKa = 11.84GRR5 pKa = 11.84AGRR8 pKa = 11.84GGGGGGRR15 pKa = 11.84DD16 pKa = 3.35RR17 pKa = 11.84GPLGGGARR25 pKa = 11.84RR26 pKa = 11.84GPGRR30 pKa = 11.84RR31 pKa = 11.84PLGSGALSGGLPHH44 pKa = 7.3ARR46 pKa = 11.84RR47 pKa = 11.84GVRR50 pKa = 11.84ARR52 pKa = 11.84GGAGRR57 pKa = 11.84LRR59 pKa = 11.84LASSFGGGGDD69 pKa = 3.49GGGGGAAAAAAAAAIAAAAAAAPRR93 pKa = 11.84SAPPRR98 pKa = 11.84RR99 pKa = 11.84PQQLWARR106 pKa = 11.84TRR108 pKa = 11.84LCTAPQPARR117 pKa = 11.84RR118 pKa = 11.84PAAA121 pKa = 3.78

Molecular weight: 11.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19884

17795

37679

24402630

34

32922

647.6

72.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.503 ± 0.011

2.116 ± 0.01

4.826 ± 0.009

7.325 ± 0.017

3.615 ± 0.009

6.319 ± 0.018

2.593 ± 0.006

4.61 ± 0.011

6.055 ± 0.015

9.743 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.208 ± 0.005

3.809 ± 0.008

6.182 ± 0.019

4.858 ± 0.011

5.464 ± 0.012

8.688 ± 0.017

5.429 ± 0.015

5.84 ± 0.011

1.153 ± 0.004

2.654 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski