Chlorella variabilis (Green alga)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae; Chlorellales; Chlorellaceae; Chlorella clade; Chlorella

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9831 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1ZK52|E1ZK52_CHLVA Expressed protein OS=Chlorella variabilis OX=554065 GN=CHLNCDRAFT_136332 PE=4 SV=1
MM1 pKa = 7.42ARR3 pKa = 11.84HH4 pKa = 6.04PAGLAAPAALLLILAAGAWTARR26 pKa = 11.84AASDD30 pKa = 3.98CPYY33 pKa = 9.6MNTSEE38 pKa = 4.5PVPATWNFQLSGPLVDD54 pKa = 6.5DD55 pKa = 4.76FMQPDD60 pKa = 3.63MMGKK64 pKa = 9.32VPGWLTTAFEE74 pKa = 4.36ASDD77 pKa = 4.68LFTIDD82 pKa = 3.65PSLTFGTYY90 pKa = 8.31EE91 pKa = 3.77YY92 pKa = 10.45RR93 pKa = 11.84DD94 pKa = 3.66QAEE97 pKa = 4.29VTSPYY102 pKa = 10.85ASYY105 pKa = 11.02GAIFNLTSPASEE117 pKa = 4.03IFGQDD122 pKa = 3.29VIDD125 pKa = 3.97EE126 pKa = 5.4LIPNDD131 pKa = 3.68EE132 pKa = 3.87IGVFKK137 pKa = 10.78RR138 pKa = 11.84QFFHH142 pKa = 7.4VSGTGSHH149 pKa = 5.59VSAVLSFVDD158 pKa = 4.74YY159 pKa = 10.14EE160 pKa = 4.29ALNITEE166 pKa = 4.33PTTLSSNSSVDD177 pKa = 3.65FAFYY181 pKa = 11.32VLGCTVQIPQAFTFSTTPPPPGLSITDD208 pKa = 3.81YY209 pKa = 11.32EE210 pKa = 4.68DD211 pKa = 3.3VAEE214 pKa = 4.77LLPSFATWVPSPDD227 pKa = 3.13SVANTTSPGFAFYY240 pKa = 10.22QYY242 pKa = 9.98WPLVVKK248 pKa = 10.84ASGEE252 pKa = 4.1PIPVFVRR259 pKa = 11.84LVTTLNSDD267 pKa = 3.46SLEE270 pKa = 4.08AAKK273 pKa = 10.63QGVNSSGTGFDD284 pKa = 3.85IYY286 pKa = 10.49WLAGDD291 pKa = 3.73EE292 pKa = 4.57VVAEE296 pKa = 4.15EE297 pKa = 4.67AGLEE301 pKa = 3.89ASLEE305 pKa = 4.19EE306 pKa = 4.24VHH308 pKa = 7.1ANLAAVLNRR317 pKa = 11.84LNDD320 pKa = 3.69TMNGGGDD327 pKa = 3.84SGPAPAPAPQPASGSAIGAAAGTPMLMALALATLAFLAA365 pKa = 4.77

Molecular weight: 38.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1Z1V6|E1Z1V6_CHLVA J domain-containing protein OS=Chlorella variabilis OX=554065 GN=CHLNCDRAFT_18104 PE=4 SV=1
MM1 pKa = 7.24CRR3 pKa = 11.84ARR5 pKa = 11.84RR6 pKa = 11.84GPQTQTWPRR15 pKa = 11.84STSRR19 pKa = 11.84AAAWAQPGRR28 pKa = 11.84QRR30 pKa = 11.84PRR32 pKa = 11.84WRR34 pKa = 11.84RR35 pKa = 11.84LRR37 pKa = 11.84RR38 pKa = 11.84GAGTRR43 pKa = 11.84PRR45 pKa = 11.84APPPPPTRR53 pKa = 11.84QRR55 pKa = 11.84APRR58 pKa = 11.84RR59 pKa = 11.84APPRR63 pKa = 11.84PRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84GAGSRR73 pKa = 11.84RR74 pKa = 11.84ASAPRR79 pKa = 11.84RR80 pKa = 11.84PSLLLRR86 pKa = 11.84RR87 pKa = 11.84RR88 pKa = 11.84RR89 pKa = 11.84RR90 pKa = 11.84RR91 pKa = 11.84GQRR94 pKa = 11.84ASPRR98 pKa = 11.84RR99 pKa = 11.84KK100 pKa = 9.51AGSSSRR106 pKa = 11.84RR107 pKa = 11.84RR108 pKa = 11.84HH109 pKa = 5.38AAPSRR114 pKa = 11.84CQAGTPSPRR123 pKa = 11.84RR124 pKa = 11.84RR125 pKa = 11.84LAAAQPRR132 pKa = 11.84PAAATPAPARR142 pKa = 11.84ALATPPCLEE151 pKa = 3.57WRR153 pKa = 11.84RR154 pKa = 11.84WCPACPARR162 pKa = 11.84RR163 pKa = 11.84RR164 pKa = 11.84RR165 pKa = 11.84RR166 pKa = 11.84RR167 pKa = 11.84GRR169 pKa = 11.84RR170 pKa = 11.84RR171 pKa = 11.84RR172 pKa = 11.84ASWRR176 pKa = 11.84RR177 pKa = 11.84PGRR180 pKa = 11.84RR181 pKa = 3.17

Molecular weight: 20.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9831

0

9831

4464018

31

11464

454.1

48.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.121 ± 0.062

1.736 ± 0.016

4.409 ± 0.017

5.908 ± 0.028

2.696 ± 0.017

9.222 ± 0.029

2.223 ± 0.01

2.54 ± 0.018

3.09 ± 0.025

9.998 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.984 ± 0.01

1.949 ± 0.015

6.406 ± 0.029

5.563 ± 0.035

6.663 ± 0.022

6.449 ± 0.024

4.243 ± 0.02

6.39 ± 0.02

1.429 ± 0.01

1.982 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski