Amino acid dipepetide frequency for Runella sp. YX9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.268AlaAla: 6.268 ± 0.073
0.686AlaCys: 0.686 ± 0.02
4.05AlaAsp: 4.05 ± 0.043
4.606AlaGlu: 4.606 ± 0.063
3.766AlaPhe: 3.766 ± 0.047
5.617AlaGly: 5.617 ± 0.062
1.344AlaHis: 1.344 ± 0.023
4.717AlaIle: 4.717 ± 0.049
4.496AlaLys: 4.496 ± 0.059
7.537AlaLeu: 7.537 ± 0.066
1.703AlaMet: 1.703 ± 0.032
3.769AlaAsn: 3.769 ± 0.043
2.806AlaPro: 2.806 ± 0.042
3.625AlaGln: 3.625 ± 0.044
2.907AlaArg: 2.907 ± 0.036
4.385AlaSer: 4.385 ± 0.046
4.577AlaThr: 4.577 ± 0.07
4.986AlaVal: 4.986 ± 0.059
0.856AlaTrp: 0.856 ± 0.024
2.901AlaTyr: 2.901 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
0.534CysAla: 0.534 ± 0.017
0.13CysCys: 0.13 ± 0.008
0.399CysAsp: 0.399 ± 0.015
0.43CysGlu: 0.43 ± 0.018
0.438CysPhe: 0.438 ± 0.016
0.625CysGly: 0.625 ± 0.022
0.214CysHis: 0.214 ± 0.01
0.546CysIle: 0.546 ± 0.018
0.387CysLys: 0.387 ± 0.013
0.802CysLeu: 0.802 ± 0.021
0.157CysMet: 0.157 ± 0.01
0.36CysAsn: 0.36 ± 0.017
0.393CysPro: 0.393 ± 0.016
0.332CysGln: 0.332 ± 0.013
0.351CysArg: 0.351 ± 0.013
0.522CysSer: 0.522 ± 0.02
0.48CysThr: 0.48 ± 0.017
0.538CysVal: 0.538 ± 0.019
0.111CysTrp: 0.111 ± 0.007
0.303CysTyr: 0.303 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
3.958AspAla: 3.958 ± 0.045
0.413AspCys: 0.413 ± 0.015
2.435AspAsp: 2.435 ± 0.063
3.214AspGlu: 3.214 ± 0.046
3.176AspPhe: 3.176 ± 0.04
3.969AspGly: 3.969 ± 0.132
0.774AspHis: 0.774 ± 0.02
3.344AspIle: 3.344 ± 0.045
3.296AspLys: 3.296 ± 0.047
4.823AspLeu: 4.823 ± 0.053
1.05AspMet: 1.05 ± 0.026
2.467AspAsn: 2.467 ± 0.048
2.043AspPro: 2.043 ± 0.04
1.442AspGln: 1.442 ± 0.026
2.143AspArg: 2.143 ± 0.035
2.837AspSer: 2.837 ± 0.044
2.683AspThr: 2.683 ± 0.066
3.306AspVal: 3.306 ± 0.045
0.905AspTrp: 0.905 ± 0.021
2.312AspTyr: 2.312 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
4.711GluAla: 4.711 ± 0.059
0.325GluCys: 0.325 ± 0.014
2.565GluAsp: 2.565 ± 0.041
3.711GluGlu: 3.711 ± 0.054
2.481GluPhe: 2.481 ± 0.037
3.778GluGly: 3.778 ± 0.042
1.036GluHis: 1.036 ± 0.024
4.26GluIle: 4.26 ± 0.05
4.669GluLys: 4.669 ± 0.066
5.822GluLeu: 5.822 ± 0.059
1.523GluMet: 1.523 ± 0.027
3.247GluAsn: 3.247 ± 0.045
1.796GluPro: 1.796 ± 0.029
2.432GluGln: 2.432 ± 0.035
2.899GluArg: 2.899 ± 0.047
2.998GluSer: 2.998 ± 0.033
3.175GluThr: 3.175 ± 0.039
4.027GluVal: 4.027 ± 0.055
0.828GluTrp: 0.828 ± 0.021
2.144GluTyr: 2.144 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
3.403PheAla: 3.403 ± 0.041
0.483PheCys: 0.483 ± 0.015
2.902PheAsp: 2.902 ± 0.038
3.064PheGlu: 3.064 ± 0.041
2.597PhePhe: 2.597 ± 0.044
3.677PheGly: 3.677 ± 0.043
0.796PheHis: 0.796 ± 0.022
2.994PheIle: 2.994 ± 0.038
2.898PheLys: 2.898 ± 0.039
4.703PheLeu: 4.703 ± 0.054
1.097PheMet: 1.097 ± 0.023
2.529PheAsn: 2.529 ± 0.046
1.869PhePro: 1.869 ± 0.034
1.769PheGln: 1.769 ± 0.028
2.172PheArg: 2.172 ± 0.036
3.769PheSer: 3.769 ± 0.051
3.067PheThr: 3.067 ± 0.044
3.408PheVal: 3.408 ± 0.043
0.741PheTrp: 0.741 ± 0.021
2.08PheTyr: 2.08 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
4.849GlyAla: 4.849 ± 0.058
0.709GlyCys: 0.709 ± 0.019
3.318GlyAsp: 3.318 ± 0.054
3.545GlyGlu: 3.545 ± 0.042
3.667GlyPhe: 3.667 ± 0.046
5.441GlyGly: 5.441 ± 0.088
1.264GlyHis: 1.264 ± 0.029
5.144GlyIle: 5.144 ± 0.056
4.988GlyLys: 4.988 ± 0.053
6.574GlyLeu: 6.574 ± 0.063
1.749GlyMet: 1.749 ± 0.029
3.611GlyAsn: 3.611 ± 0.058
1.759GlyPro: 1.759 ± 0.032
2.709GlyGln: 2.709 ± 0.043
2.948GlyArg: 2.948 ± 0.037
4.174GlySer: 4.174 ± 0.057
4.672GlyThr: 4.672 ± 0.104
4.877GlyVal: 4.877 ± 0.052
1.104GlyTrp: 1.104 ± 0.024
2.964GlyTyr: 2.964 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
1.185HisAla: 1.185 ± 0.023
0.214HisCys: 0.214 ± 0.01
0.859HisAsp: 0.859 ± 0.021
1.047HisGlu: 1.047 ± 0.023
1.218HisPhe: 1.218 ± 0.024
1.167HisGly: 1.167 ± 0.026
0.521HisHis: 0.521 ± 0.017
1.156HisIle: 1.156 ± 0.024
0.928HisLys: 0.928 ± 0.021
2.041HisLeu: 2.041 ± 0.029
0.251HisMet: 0.251 ± 0.01
0.845HisAsn: 0.845 ± 0.021
1.095HisPro: 1.095 ± 0.025
0.838HisGln: 0.838 ± 0.021
0.937HisArg: 0.937 ± 0.02
1.09HisSer: 1.09 ± 0.025
0.969HisThr: 0.969 ± 0.021
1.044HisVal: 1.044 ± 0.024
0.318HisTrp: 0.318 ± 0.011
0.885HisTyr: 0.885 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
5.161IleAla: 5.161 ± 0.055
0.618IleCys: 0.618 ± 0.019
3.629IleAsp: 3.629 ± 0.044
4.227IleGlu: 4.227 ± 0.057
2.864IlePhe: 2.864 ± 0.037
4.699IleGly: 4.699 ± 0.051
1.278IleHis: 1.278 ± 0.023
3.872IleIle: 3.872 ± 0.051
3.871IleLys: 3.871 ± 0.041
5.692IleLeu: 5.692 ± 0.058
1.211IleMet: 1.211 ± 0.025
3.415IleAsn: 3.415 ± 0.042
3.023IlePro: 3.023 ± 0.041
2.535IleGln: 2.535 ± 0.035
2.988IleArg: 2.988 ± 0.044
4.275IleSer: 4.275 ± 0.043
4.037IleThr: 4.037 ± 0.052
4.25IleVal: 4.25 ± 0.046
0.765IleTrp: 0.765 ± 0.024
2.298IleTyr: 2.298 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
5.036LysAla: 5.036 ± 0.057
0.329LysCys: 0.329 ± 0.014
3.343LysAsp: 3.343 ± 0.047
4.192LysGlu: 4.192 ± 0.051
2.216LysPhe: 2.216 ± 0.036
4.337LysGly: 4.337 ± 0.047
1.118LysHis: 1.118 ± 0.023
4.573LysIle: 4.573 ± 0.049
4.725LysLys: 4.725 ± 0.059
5.519LysLeu: 5.519 ± 0.053
1.812LysMet: 1.812 ± 0.032
3.728LysAsn: 3.728 ± 0.045
2.816LysPro: 2.816 ± 0.045
2.461LysGln: 2.461 ± 0.042
2.77LysArg: 2.77 ± 0.035
3.683LysSer: 3.683 ± 0.04
4.042LysThr: 4.042 ± 0.046
4.224LysVal: 4.224 ± 0.056
0.882LysTrp: 0.882 ± 0.021
2.474LysTyr: 2.474 ± 0.038
0.0LysXaa: 0.0 ± 0.0
Leu
7.203LeuAla: 7.203 ± 0.066
0.798LeuCys: 0.798 ± 0.019
4.526LeuAsp: 4.526 ± 0.064
5.255LeuGlu: 5.255 ± 0.065
4.911LeuPhe: 4.911 ± 0.059
6.331LeuGly: 6.331 ± 0.061
1.738LeuHis: 1.738 ± 0.029
5.957LeuIle: 5.957 ± 0.069
6.771LeuLys: 6.771 ± 0.061
9.647LeuLeu: 9.647 ± 0.105
2.263LeuMet: 2.263 ± 0.035
5.103LeuAsn: 5.103 ± 0.056
4.629LeuPro: 4.629 ± 0.043
3.721LeuGln: 3.721 ± 0.051
4.414LeuArg: 4.414 ± 0.048
6.766LeuSer: 6.766 ± 0.06
6.22LeuThr: 6.22 ± 0.057
5.873LeuVal: 5.873 ± 0.059
1.194LeuTrp: 1.194 ± 0.023
3.308LeuTyr: 3.308 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
1.87MetAla: 1.87 ± 0.033
0.149MetCys: 0.149 ± 0.008
1.051MetAsp: 1.051 ± 0.024
1.197MetGlu: 1.197 ± 0.026
0.755MetPhe: 0.755 ± 0.019
1.681MetGly: 1.681 ± 0.03
0.413MetHis: 0.413 ± 0.016
1.268MetIle: 1.268 ± 0.026
1.898MetLys: 1.898 ± 0.03
2.169MetLeu: 2.169 ± 0.032
0.607MetMet: 0.607 ± 0.016
1.169MetAsn: 1.169 ± 0.02
1.108MetPro: 1.108 ± 0.023
0.854MetGln: 0.854 ± 0.02
1.044MetArg: 1.044 ± 0.02
1.36MetSer: 1.36 ± 0.024
1.352MetThr: 1.352 ± 0.027
1.437MetVal: 1.437 ± 0.026
0.208MetTrp: 0.208 ± 0.011
0.706MetTyr: 0.706 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
4.035AsnAla: 4.035 ± 0.052
0.395AsnCys: 0.395 ± 0.017
2.691AsnAsp: 2.691 ± 0.053
2.905AsnGlu: 2.905 ± 0.039
2.541AsnPhe: 2.541 ± 0.041
4.081AsnGly: 4.081 ± 0.06
1.047AsnHis: 1.047 ± 0.023
3.206AsnIle: 3.206 ± 0.043
2.652AsnLys: 2.652 ± 0.039
4.642AsnLeu: 4.642 ± 0.058
0.943AsnMet: 0.943 ± 0.02
2.838AsnAsn: 2.838 ± 0.049
3.017AsnPro: 3.017 ± 0.043
2.34AsnGln: 2.34 ± 0.035
2.564AsnArg: 2.564 ± 0.039
2.956AsnSer: 2.956 ± 0.05
3.161AsnThr: 3.161 ± 0.057
3.33AsnVal: 3.33 ± 0.047
0.723AsnTrp: 0.723 ± 0.019
2.318AsnTyr: 2.318 ± 0.038
0.0AsnXaa: 0.0 ± 0.0
Pro
3.3ProAla: 3.3 ± 0.037
0.238ProCys: 0.238 ± 0.011
2.671ProAsp: 2.671 ± 0.045
2.977ProGlu: 2.977 ± 0.039
2.193ProPhe: 2.193 ± 0.035
2.169ProGly: 2.169 ± 0.039
0.854ProHis: 0.854 ± 0.022
2.629ProIle: 2.629 ± 0.035
2.58ProLys: 2.58 ± 0.042
3.81ProLeu: 3.81 ± 0.047
0.902ProMet: 0.902 ± 0.024
2.481ProAsn: 2.481 ± 0.042
1.377ProPro: 1.377 ± 0.035
1.718ProGln: 1.718 ± 0.028
1.365ProArg: 1.365 ± 0.028
2.687ProSer: 2.687 ± 0.037
2.835ProThr: 2.835 ± 0.046
3.037ProVal: 3.037 ± 0.041
0.475ProTrp: 0.475 ± 0.017
1.63ProTyr: 1.63 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
2.835GlnAla: 2.835 ± 0.04
0.227GlnCys: 0.227 ± 0.011
1.536GlnAsp: 1.536 ± 0.029
2.092GlnGlu: 2.092 ± 0.04
1.797GlnPhe: 1.797 ± 0.031
2.253GlnGly: 2.253 ± 0.037
0.81GlnHis: 0.81 ± 0.022
2.652GlnIle: 2.652 ± 0.038
3.22GlnLys: 3.22 ± 0.051
4.22GlnLeu: 4.22 ± 0.05
0.942GlnMet: 0.942 ± 0.021
2.401GlnAsn: 2.401 ± 0.037
1.733GlnPro: 1.733 ± 0.029
1.988GlnGln: 1.988 ± 0.031
2.035GlnArg: 2.035 ± 0.033
2.424GlnSer: 2.424 ± 0.036
2.689GlnThr: 2.689 ± 0.041
2.404GlnVal: 2.404 ± 0.033
0.615GlnTrp: 0.615 ± 0.02
1.467GlnTyr: 1.467 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
2.882ArgAla: 2.882 ± 0.04
0.278ArgCys: 0.278 ± 0.012
2.099ArgAsp: 2.099 ± 0.034
2.605ArgGlu: 2.605 ± 0.039
2.462ArgPhe: 2.462 ± 0.037
2.533ArgGly: 2.533 ± 0.034
0.853ArgHis: 0.853 ± 0.021
3.228ArgIle: 3.228 ± 0.041
2.831ArgLys: 2.831 ± 0.043
4.532ArgLeu: 4.532 ± 0.049
1.15ArgMet: 1.15 ± 0.025
2.37ArgAsn: 2.37 ± 0.035
1.734ArgPro: 1.734 ± 0.025
2.016ArgGln: 2.016 ± 0.036
2.163ArgArg: 2.163 ± 0.038
2.377ArgSer: 2.377 ± 0.037
2.449ArgThr: 2.449 ± 0.035
2.889ArgVal: 2.889 ± 0.04
0.688ArgTrp: 0.688 ± 0.021
1.979ArgTyr: 1.979 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
4.506SerAla: 4.506 ± 0.053
0.548SerCys: 0.548 ± 0.016
2.997SerAsp: 2.997 ± 0.047
3.255SerGlu: 3.255 ± 0.037
3.472SerPhe: 3.472 ± 0.048
4.647SerGly: 4.647 ± 0.065
1.094SerHis: 1.094 ± 0.022
4.111SerIle: 4.111 ± 0.044
3.468SerLys: 3.468 ± 0.043
6.351SerLeu: 6.351 ± 0.065
1.3SerMet: 1.3 ± 0.024
3.008SerAsn: 3.008 ± 0.054
2.723SerPro: 2.723 ± 0.036
2.314SerGln: 2.314 ± 0.032
2.461SerArg: 2.461 ± 0.034
3.822SerSer: 3.822 ± 0.055
3.778SerThr: 3.778 ± 0.05
4.283SerVal: 4.283 ± 0.05
0.782SerTrp: 0.782 ± 0.019
2.551SerTyr: 2.551 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
4.861ThrAla: 4.861 ± 0.064
0.405ThrCys: 0.405 ± 0.018
3.406ThrAsp: 3.406 ± 0.084
3.143ThrGlu: 3.143 ± 0.04
3.127ThrPhe: 3.127 ± 0.042
4.668ThrGly: 4.668 ± 0.076
1.176ThrHis: 1.176 ± 0.023
3.926ThrIle: 3.926 ± 0.057
3.363ThrLys: 3.363 ± 0.047
6.141ThrLeu: 6.141 ± 0.064
1.056ThrMet: 1.056 ± 0.024
3.061ThrAsn: 3.061 ± 0.05
3.222ThrPro: 3.222 ± 0.048
2.385ThrGln: 2.385 ± 0.035
2.137ThrArg: 2.137 ± 0.03
3.584ThrSer: 3.584 ± 0.046
3.93ThrThr: 3.93 ± 0.065
4.352ThrVal: 4.352 ± 0.069
0.754ThrTrp: 0.754 ± 0.022
2.411ThrTyr: 2.411 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
5.201ValAla: 5.201 ± 0.059
0.636ValCys: 0.636 ± 0.019
3.236ValAsp: 3.236 ± 0.046
3.935ValGlu: 3.935 ± 0.047
3.334ValPhe: 3.334 ± 0.043
4.695ValGly: 4.695 ± 0.054
1.144ValHis: 1.144 ± 0.024
4.246ValIle: 4.246 ± 0.046
4.204ValLys: 4.204 ± 0.053
6.229ValLeu: 6.229 ± 0.059
1.501ValMet: 1.501 ± 0.026
3.3ValAsn: 3.3 ± 0.048
2.708ValPro: 2.708 ± 0.037
2.283ValGln: 2.283 ± 0.034
2.982ValArg: 2.982 ± 0.041
4.544ValSer: 4.544 ± 0.054
3.983ValThr: 3.983 ± 0.054
4.83ValVal: 4.83 ± 0.058
0.899ValTrp: 0.899 ± 0.024
2.453ValTyr: 2.453 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
0.98TrpAla: 0.98 ± 0.02
0.114TrpCys: 0.114 ± 0.008
0.691TrpAsp: 0.691 ± 0.019
0.784TrpGlu: 0.784 ± 0.02
0.654TrpPhe: 0.654 ± 0.019
0.984TrpGly: 0.984 ± 0.02
0.303TrpHis: 0.303 ± 0.011
0.779TrpIle: 0.779 ± 0.019
0.903TrpLys: 0.903 ± 0.025
1.434TrpLeu: 1.434 ± 0.027
0.401TrpMet: 0.401 ± 0.014
0.705TrpAsn: 0.705 ± 0.017
0.381TrpPro: 0.381 ± 0.013
0.718TrpGln: 0.718 ± 0.02
0.651TrpArg: 0.651 ± 0.019
0.818TrpSer: 0.818 ± 0.025
0.708TrpThr: 0.708 ± 0.018
0.924TrpVal: 0.924 ± 0.023
0.245TrpTrp: 0.245 ± 0.012
0.504TrpTyr: 0.504 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.821TyrAla: 2.821 ± 0.039
0.36TyrCys: 0.36 ± 0.015
2.185TyrAsp: 2.185 ± 0.038
2.244TyrGlu: 2.244 ± 0.034
2.349TyrPhe: 2.349 ± 0.035
2.713TyrGly: 2.713 ± 0.04
0.832TyrHis: 0.832 ± 0.018
2.104TyrIle: 2.104 ± 0.034
2.132TyrLys: 2.132 ± 0.032
3.941TyrLeu: 3.941 ± 0.045
0.676TyrMet: 0.676 ± 0.018
1.973TyrAsn: 1.973 ± 0.034
1.693TyrPro: 1.693 ± 0.031
1.86TyrGln: 1.86 ± 0.028
2.187TyrArg: 2.187 ± 0.032
2.399TyrSer: 2.399 ± 0.036
2.301TyrThr: 2.301 ± 0.037
2.326TyrVal: 2.326 ± 0.035
0.592TyrTrp: 0.592 ± 0.016
1.654TyrTyr: 1.654 ± 0.029
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.002XaaXaa: 0.002 ± 0.001
Statistics based on 5856 proteins (2137984 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski