Cupriavidus gilardii J11

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Cupriavidus; Cupriavidus gilardii

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4347 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A562B5F7|A0A562B5F7_9BURK Putative addiction module killer protein OS=Cupriavidus gilardii J11 OX=936133 GN=L602_000500001030 PE=4 SV=1
MM1 pKa = 7.08TFVVTEE7 pKa = 3.75ACVRR11 pKa = 11.84CKK13 pKa = 10.27YY14 pKa = 8.57TDD16 pKa = 3.76CVSVCPVEE24 pKa = 4.99CFHH27 pKa = 7.07EE28 pKa = 5.62GPDD31 pKa = 4.12FLVIDD36 pKa = 4.55PDD38 pKa = 3.8VCIDD42 pKa = 4.04CGVCVPEE49 pKa = 4.74CPIGAISADD58 pKa = 3.36TDD60 pKa = 3.68LPEE63 pKa = 4.15AQRR66 pKa = 11.84DD67 pKa = 4.14FIEE70 pKa = 4.23INARR74 pKa = 11.84LAARR78 pKa = 11.84WPVITAARR86 pKa = 11.84DD87 pKa = 3.58PLPDD91 pKa = 3.99AEE93 pKa = 4.22QWADD97 pKa = 3.34VAGKK101 pKa = 9.7RR102 pKa = 11.84QYY104 pKa = 11.25LDD106 pKa = 3.15EE107 pKa = 5.19ALAALASDD115 pKa = 4.11

Molecular weight: 12.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A562BK62|A0A562BK62_9BURK Uncharacterized protein OS=Cupriavidus gilardii J11 OX=936133 GN=L602_002500000820 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4347

0

4347

1415191

23

2848

325.6

35.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.249 ± 0.052

0.96 ± 0.012

5.545 ± 0.024

5.181 ± 0.034

3.335 ± 0.025

8.486 ± 0.038

2.309 ± 0.018

4.409 ± 0.026

2.748 ± 0.032

10.342 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.548 ± 0.014

2.467 ± 0.023

5.531 ± 0.03

3.659 ± 0.022

7.796 ± 0.037

5.039 ± 0.025

5.067 ± 0.023

7.629 ± 0.028

1.392 ± 0.015

2.275 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski