Halovirus HCTV-2

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified archaeal dsDNA viruses; Haloviruses

Average proteome isoelectric point is 5.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4TNM4|R4TNM4_9VIRU Uncharacterized protein OS=Halovirus HCTV-2 OX=1273747 GN=64 PE=4 SV=1
MM1 pKa = 7.49ALEE4 pKa = 4.88PIGDD8 pKa = 3.49IGVAALTEE16 pKa = 4.31QVGSEE21 pKa = 4.12TLTWATAAEE30 pKa = 3.87WDD32 pKa = 4.07ANVSEE37 pKa = 4.48TGVVHH42 pKa = 7.14EE43 pKa = 5.32DD44 pKa = 3.15FGDD47 pKa = 3.74RR48 pKa = 11.84DD49 pKa = 3.42ATRR52 pKa = 11.84IEE54 pKa = 4.44LGFPSTDD61 pKa = 3.23PVNSGLAYY69 pKa = 10.29YY70 pKa = 10.27YY71 pKa = 10.11PLDD74 pKa = 4.18EE75 pKa = 5.09ASGPATDD82 pKa = 3.91ATGNGPNGSLSGSTSLGNSGTLGTTCIDD110 pKa = 3.71FPGGGGDD117 pKa = 3.56TLDD120 pKa = 4.86FPDD123 pKa = 5.07DD124 pKa = 4.16PVWDD128 pKa = 3.5ITGDD132 pKa = 3.23ITVVHH137 pKa = 7.0AIYY140 pKa = 9.82WRR142 pKa = 11.84GGNDD146 pKa = 3.05SSSWQGLTSKK156 pKa = 10.58GGNSSPWAIAYY167 pKa = 10.02VDD169 pKa = 4.02GYY171 pKa = 10.54YY172 pKa = 10.49VQFQVDD178 pKa = 3.66GTRR181 pKa = 11.84YY182 pKa = 8.57DD183 pKa = 2.69TGYY186 pKa = 11.25DD187 pKa = 3.6LRR189 pKa = 11.84NTQNTWALHH198 pKa = 6.6AYY200 pKa = 9.63QLDD203 pKa = 3.93SGSIRR208 pKa = 11.84HH209 pKa = 5.21QVNGGNEE216 pKa = 4.0HH217 pKa = 7.09DD218 pKa = 3.96TTNSAEE224 pKa = 4.52AINNTTSDD232 pKa = 3.75EE233 pKa = 4.34FVLGARR239 pKa = 11.84KK240 pKa = 10.12SGGDD244 pKa = 3.51EE245 pKa = 3.95VNALVDD251 pKa = 3.62DD252 pKa = 4.73FFVFDD257 pKa = 3.93YY258 pKa = 10.97AISDD262 pKa = 3.54TRR264 pKa = 11.84LTDD267 pKa = 5.63LYY269 pKa = 10.82DD270 pKa = 3.27AWSTGTLTTATKK282 pKa = 9.28TVSSSGQPDD291 pKa = 4.03LQNLVYY297 pKa = 10.41SLNGEE302 pKa = 4.51SITLDD307 pKa = 3.54VIGSPGTASEE317 pKa = 4.29EE318 pKa = 4.55TVTQALDD325 pKa = 3.55GSSSYY330 pKa = 10.79TLTWADD336 pKa = 2.86SHH338 pKa = 6.05TDD340 pKa = 3.61FRR342 pKa = 11.84VRR344 pKa = 11.84AEE346 pKa = 4.42LTTSDD351 pKa = 3.79PTVSPTVSQIEE362 pKa = 4.39LVTT365 pKa = 3.71

Molecular weight: 38.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4TNQ4|R4TNQ4_9VIRU Uncharacterized protein OS=Halovirus HCTV-2 OX=1273747 GN=85 PE=4 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84GARR5 pKa = 11.84PVRR8 pKa = 11.84TRR10 pKa = 11.84TRR12 pKa = 11.84SVTRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84SLVDD22 pKa = 3.33AGRR25 pKa = 11.84GRR27 pKa = 11.84LGRR30 pKa = 11.84ALRR33 pKa = 11.84PLAHH37 pKa = 7.37RR38 pKa = 11.84SPTTHH43 pKa = 7.92PLRR46 pKa = 11.84PPPSVRR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84ALFSPFAA61 pKa = 5.27

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

17670

31

786

205.5

22.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.655 ± 0.296

1.047 ± 0.161

8.149 ± 0.235

9.502 ± 0.436

2.315 ± 0.19

9.225 ± 0.375

2.394 ± 0.198

2.716 ± 0.219

2.258 ± 0.185

6.927 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.851 ± 0.127

2.666 ± 0.167

4.884 ± 0.187

5.059 ± 0.248

6.44 ± 0.382

5.806 ± 0.297

6.786 ± 0.325

7.691 ± 0.241

1.89 ± 0.111

2.739 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski