Amino acid dipepetide frequency for Mycobacterium phage Sibs6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.028AlaAla: 13.028 ± 1.224
0.696AlaCys: 0.696 ± 0.193
6.767AlaAsp: 6.767 ± 0.729
6.388AlaGlu: 6.388 ± 0.829
2.972AlaPhe: 2.972 ± 0.502
8.222AlaGly: 8.222 ± 0.964
1.708AlaHis: 1.708 ± 0.41
3.668AlaIle: 3.668 ± 0.483
4.49AlaLys: 4.49 ± 0.658
9.233AlaLeu: 9.233 ± 0.909
2.656AlaMet: 2.656 ± 0.396
2.466AlaAsn: 2.466 ± 0.357
5.186AlaPro: 5.186 ± 0.731
2.783AlaGln: 2.783 ± 0.4
6.324AlaArg: 6.324 ± 0.666
4.806AlaSer: 4.806 ± 0.528
5.629AlaThr: 5.629 ± 0.659
7.842AlaVal: 7.842 ± 0.809
1.771AlaTrp: 1.771 ± 0.387
2.783AlaTyr: 2.783 ± 0.377
0.0AlaXaa: 0.0 ± 0.0
Cys
0.822CysAla: 0.822 ± 0.298
0.0CysCys: 0.0 ± 0.0
0.506CysAsp: 0.506 ± 0.186
0.759CysGlu: 0.759 ± 0.252
0.253CysPhe: 0.253 ± 0.129
0.696CysGly: 0.696 ± 0.238
0.316CysHis: 0.316 ± 0.13
0.253CysIle: 0.253 ± 0.132
0.253CysLys: 0.253 ± 0.106
0.19CysLeu: 0.19 ± 0.111
0.19CysMet: 0.19 ± 0.107
0.443CysAsn: 0.443 ± 0.18
0.379CysPro: 0.379 ± 0.146
0.19CysGln: 0.19 ± 0.098
0.443CysArg: 0.443 ± 0.151
0.316CysSer: 0.316 ± 0.145
0.253CysThr: 0.253 ± 0.129
0.379CysVal: 0.379 ± 0.16
0.126CysTrp: 0.126 ± 0.089
0.126CysTyr: 0.126 ± 0.112
0.0CysXaa: 0.0 ± 0.0
Asp
6.008AspAla: 6.008 ± 0.618
0.506AspCys: 0.506 ± 0.18
4.806AspAsp: 4.806 ± 0.604
3.668AspGlu: 3.668 ± 0.514
2.466AspPhe: 2.466 ± 0.338
6.071AspGly: 6.071 ± 0.606
1.265AspHis: 1.265 ± 0.272
2.846AspIle: 2.846 ± 0.404
3.036AspLys: 3.036 ± 0.483
6.767AspLeu: 6.767 ± 0.803
1.391AspMet: 1.391 ± 0.244
1.834AspAsn: 1.834 ± 0.296
4.554AspPro: 4.554 ± 0.48
1.581AspGln: 1.581 ± 0.326
3.542AspArg: 3.542 ± 0.41
3.036AspSer: 3.036 ± 0.45
4.174AspThr: 4.174 ± 0.48
4.68AspVal: 4.68 ± 0.577
1.708AspTrp: 1.708 ± 0.317
1.961AspTyr: 1.961 ± 0.367
0.0AspXaa: 0.0 ± 0.0
Glu
6.261GluAla: 6.261 ± 0.662
0.253GluCys: 0.253 ± 0.159
4.933GluAsp: 4.933 ± 0.541
4.933GluGlu: 4.933 ± 0.635
2.087GluPhe: 2.087 ± 0.375
4.364GluGly: 4.364 ± 0.48
1.581GluHis: 1.581 ± 0.375
3.605GluIle: 3.605 ± 0.496
2.403GluLys: 2.403 ± 0.475
6.957GluLeu: 6.957 ± 0.599
1.518GluMet: 1.518 ± 0.311
1.644GluAsn: 1.644 ± 0.327
2.53GluPro: 2.53 ± 0.442
2.53GluGln: 2.53 ± 0.395
4.174GluArg: 4.174 ± 0.628
3.162GluSer: 3.162 ± 0.388
3.731GluThr: 3.731 ± 0.41
5.186GluVal: 5.186 ± 0.59
1.581GluTrp: 1.581 ± 0.311
2.53GluTyr: 2.53 ± 0.382
0.0GluXaa: 0.0 ± 0.0
Phe
2.656PheAla: 2.656 ± 0.505
0.253PheCys: 0.253 ± 0.158
3.036PheAsp: 3.036 ± 0.38
1.961PheGlu: 1.961 ± 0.322
0.759PhePhe: 0.759 ± 0.218
3.668PheGly: 3.668 ± 0.525
0.822PheHis: 0.822 ± 0.226
1.265PheIle: 1.265 ± 0.265
1.265PheLys: 1.265 ± 0.277
2.403PheLeu: 2.403 ± 0.451
0.759PheMet: 0.759 ± 0.231
1.202PheAsn: 1.202 ± 0.293
1.708PhePro: 1.708 ± 0.322
1.012PheGln: 1.012 ± 0.278
2.024PheArg: 2.024 ± 0.369
1.771PheSer: 1.771 ± 0.277
2.214PheThr: 2.214 ± 0.377
1.897PheVal: 1.897 ± 0.399
0.569PheTrp: 0.569 ± 0.158
0.759PheTyr: 0.759 ± 0.207
0.0PheXaa: 0.0 ± 0.0
Gly
7.589GlyAla: 7.589 ± 1.502
0.885GlyCys: 0.885 ± 0.26
5.755GlyAsp: 5.755 ± 0.557
4.49GlyGlu: 4.49 ± 0.591
2.846GlyPhe: 2.846 ± 0.458
10.372GlyGly: 10.372 ± 3.23
1.771GlyHis: 1.771 ± 0.349
4.996GlyIle: 4.996 ± 0.668
3.352GlyLys: 3.352 ± 0.49
7.652GlyLeu: 7.652 ± 0.797
1.834GlyMet: 1.834 ± 0.387
3.668GlyAsn: 3.668 ± 0.469
3.858GlyPro: 3.858 ± 0.601
2.403GlyGln: 2.403 ± 0.402
5.059GlyArg: 5.059 ± 0.602
5.692GlySer: 5.692 ± 0.896
4.996GlyThr: 4.996 ± 0.61
5.312GlyVal: 5.312 ± 0.568
2.403GlyTrp: 2.403 ± 0.373
2.783GlyTyr: 2.783 ± 0.36
0.0GlyXaa: 0.0 ± 0.0
His
1.961HisAla: 1.961 ± 0.41
0.253HisCys: 0.253 ± 0.151
1.202HisAsp: 1.202 ± 0.238
1.518HisGlu: 1.518 ± 0.324
0.822HisPhe: 0.822 ± 0.227
1.644HisGly: 1.644 ± 0.38
0.759HisHis: 0.759 ± 0.216
0.885HisIle: 0.885 ± 0.218
1.012HisLys: 1.012 ± 0.31
1.328HisLeu: 1.328 ± 0.257
0.126HisMet: 0.126 ± 0.091
0.379HisAsn: 0.379 ± 0.135
1.075HisPro: 1.075 ± 0.248
1.138HisGln: 1.138 ± 0.308
1.581HisArg: 1.581 ± 0.281
0.759HisSer: 0.759 ± 0.206
1.202HisThr: 1.202 ± 0.308
1.644HisVal: 1.644 ± 0.292
0.569HisTrp: 0.569 ± 0.172
0.759HisTyr: 0.759 ± 0.24
0.0HisXaa: 0.0 ± 0.0
Ile
5.755IleAla: 5.755 ± 0.672
0.19IleCys: 0.19 ± 0.11
3.415IleAsp: 3.415 ± 0.394
3.605IleGlu: 3.605 ± 0.448
0.822IlePhe: 0.822 ± 0.189
4.174IleGly: 4.174 ± 0.577
0.822IleHis: 0.822 ± 0.217
1.834IleIle: 1.834 ± 0.365
1.897IleLys: 1.897 ± 0.31
3.605IleLeu: 3.605 ± 0.498
0.696IleMet: 0.696 ± 0.195
1.834IleAsn: 1.834 ± 0.296
2.909IlePro: 2.909 ± 0.366
1.581IleGln: 1.581 ± 0.376
3.605IleArg: 3.605 ± 0.54
3.289IleSer: 3.289 ± 0.504
3.289IleThr: 3.289 ± 0.412
3.225IleVal: 3.225 ± 0.464
0.696IleTrp: 0.696 ± 0.17
1.834IleTyr: 1.834 ± 0.285
0.0IleXaa: 0.0 ± 0.0
Lys
3.605LysAla: 3.605 ± 0.539
0.253LysCys: 0.253 ± 0.134
2.53LysAsp: 2.53 ± 0.4
1.961LysGlu: 1.961 ± 0.338
1.644LysPhe: 1.644 ± 0.272
2.656LysGly: 2.656 ± 0.31
1.265LysHis: 1.265 ± 0.341
2.403LysIle: 2.403 ± 0.44
2.277LysLys: 2.277 ± 0.473
3.542LysLeu: 3.542 ± 0.441
0.885LysMet: 0.885 ± 0.204
1.265LysAsn: 1.265 ± 0.289
2.466LysPro: 2.466 ± 0.469
1.961LysGln: 1.961 ± 0.377
3.668LysArg: 3.668 ± 0.696
2.34LysSer: 2.34 ± 0.376
2.214LysThr: 2.214 ± 0.454
3.099LysVal: 3.099 ± 0.461
0.696LysTrp: 0.696 ± 0.233
1.138LysTyr: 1.138 ± 0.302
0.0LysXaa: 0.0 ± 0.0
Leu
9.107LeuAla: 9.107 ± 1.005
0.19LeuCys: 0.19 ± 0.103
6.198LeuAsp: 6.198 ± 0.589
5.376LeuGlu: 5.376 ± 0.669
2.087LeuPhe: 2.087 ± 0.366
7.146LeuGly: 7.146 ± 0.709
1.328LeuHis: 1.328 ± 0.314
4.933LeuIle: 4.933 ± 0.563
4.111LeuLys: 4.111 ± 0.422
5.755LeuLeu: 5.755 ± 0.513
1.644LeuMet: 1.644 ± 0.297
2.783LeuAsn: 2.783 ± 0.426
5.565LeuPro: 5.565 ± 0.57
2.719LeuGln: 2.719 ± 0.52
6.198LeuArg: 6.198 ± 0.555
5.629LeuSer: 5.629 ± 0.556
5.882LeuThr: 5.882 ± 0.443
4.806LeuVal: 4.806 ± 0.577
1.138LeuTrp: 1.138 ± 0.354
2.277LeuTyr: 2.277 ± 0.412
0.0LeuXaa: 0.0 ± 0.0
Met
2.53MetAla: 2.53 ± 0.366
0.0MetCys: 0.0 ± 0.0
1.202MetAsp: 1.202 ± 0.287
1.455MetGlu: 1.455 ± 0.339
0.632MetPhe: 0.632 ± 0.158
1.202MetGly: 1.202 ± 0.275
0.379MetHis: 0.379 ± 0.171
0.569MetIle: 0.569 ± 0.179
1.265MetLys: 1.265 ± 0.287
1.202MetLeu: 1.202 ± 0.249
0.126MetMet: 0.126 ± 0.102
0.822MetAsn: 0.822 ± 0.201
1.202MetPro: 1.202 ± 0.235
0.506MetGln: 0.506 ± 0.148
1.138MetArg: 1.138 ± 0.233
2.593MetSer: 2.593 ± 0.393
2.087MetThr: 2.087 ± 0.316
1.012MetVal: 1.012 ± 0.261
0.253MetTrp: 0.253 ± 0.123
0.569MetTyr: 0.569 ± 0.218
0.0MetXaa: 0.0 ± 0.0
Asn
3.289AsnAla: 3.289 ± 0.49
0.253AsnCys: 0.253 ± 0.169
2.15AsnAsp: 2.15 ± 0.37
2.087AsnGlu: 2.087 ± 0.362
1.012AsnPhe: 1.012 ± 0.25
3.415AsnGly: 3.415 ± 0.481
0.885AsnHis: 0.885 ± 0.224
1.644AsnIle: 1.644 ± 0.373
0.443AsnLys: 0.443 ± 0.176
2.087AsnLeu: 2.087 ± 0.353
0.632AsnMet: 0.632 ± 0.173
0.949AsnAsn: 0.949 ± 0.249
2.783AsnPro: 2.783 ± 0.375
1.138AsnGln: 1.138 ± 0.245
1.265AsnArg: 1.265 ± 0.347
1.518AsnSer: 1.518 ± 0.385
2.15AsnThr: 2.15 ± 0.328
2.593AsnVal: 2.593 ± 0.436
0.822AsnTrp: 0.822 ± 0.195
1.075AsnTyr: 1.075 ± 0.28
0.0AsnXaa: 0.0 ± 0.0
Pro
5.502ProAla: 5.502 ± 0.708
0.379ProCys: 0.379 ± 0.151
4.048ProAsp: 4.048 ± 0.486
4.617ProGlu: 4.617 ± 0.452
2.277ProPhe: 2.277 ± 0.374
5.502ProGly: 5.502 ± 0.689
0.885ProHis: 0.885 ± 0.239
2.593ProIle: 2.593 ± 0.384
2.087ProLys: 2.087 ± 0.299
4.617ProLeu: 4.617 ± 0.544
0.949ProMet: 0.949 ± 0.241
1.708ProAsn: 1.708 ± 0.333
3.162ProPro: 3.162 ± 0.484
1.391ProGln: 1.391 ± 0.295
2.783ProArg: 2.783 ± 0.458
3.542ProSer: 3.542 ± 0.41
3.542ProThr: 3.542 ± 0.539
3.542ProVal: 3.542 ± 0.438
0.885ProTrp: 0.885 ± 0.268
1.328ProTyr: 1.328 ± 0.347
0.0ProXaa: 0.0 ± 0.0
Gln
3.036GlnAla: 3.036 ± 0.479
0.126GlnCys: 0.126 ± 0.09
1.391GlnAsp: 1.391 ± 0.347
1.834GlnGlu: 1.834 ± 0.355
1.138GlnPhe: 1.138 ± 0.249
2.277GlnGly: 2.277 ± 0.368
0.696GlnHis: 0.696 ± 0.176
2.277GlnIle: 2.277 ± 0.421
1.455GlnLys: 1.455 ± 0.319
3.478GlnLeu: 3.478 ± 0.417
1.012GlnMet: 1.012 ± 0.264
0.696GlnAsn: 0.696 ± 0.209
1.961GlnPro: 1.961 ± 0.359
1.708GlnGln: 1.708 ± 0.396
1.834GlnArg: 1.834 ± 0.426
1.518GlnSer: 1.518 ± 0.293
1.644GlnThr: 1.644 ± 0.292
2.403GlnVal: 2.403 ± 0.328
0.632GlnTrp: 0.632 ± 0.147
0.569GlnTyr: 0.569 ± 0.166
0.0GlnXaa: 0.0 ± 0.0
Arg
5.439ArgAla: 5.439 ± 0.582
0.822ArgCys: 0.822 ± 0.309
3.352ArgAsp: 3.352 ± 0.454
5.123ArgGlu: 5.123 ± 0.649
1.834ArgPhe: 1.834 ± 0.366
4.68ArgGly: 4.68 ± 0.638
1.265ArgHis: 1.265 ± 0.312
3.542ArgIle: 3.542 ± 0.427
3.225ArgLys: 3.225 ± 0.608
6.008ArgLeu: 6.008 ± 0.679
1.897ArgMet: 1.897 ± 0.386
2.593ArgAsn: 2.593 ± 0.49
2.846ArgPro: 2.846 ± 0.472
1.834ArgGln: 1.834 ± 0.38
5.629ArgArg: 5.629 ± 0.744
3.795ArgSer: 3.795 ± 0.556
3.162ArgThr: 3.162 ± 0.502
5.249ArgVal: 5.249 ± 0.462
1.391ArgTrp: 1.391 ± 0.283
1.708ArgTyr: 1.708 ± 0.273
0.0ArgXaa: 0.0 ± 0.0
Ser
6.071SerAla: 6.071 ± 0.761
0.506SerCys: 0.506 ± 0.201
3.162SerAsp: 3.162 ± 0.448
3.605SerGlu: 3.605 ± 0.451
2.214SerPhe: 2.214 ± 0.434
6.071SerGly: 6.071 ± 0.936
1.391SerHis: 1.391 ± 0.297
2.719SerIle: 2.719 ± 0.424
2.403SerLys: 2.403 ± 0.378
4.743SerLeu: 4.743 ± 0.497
1.391SerMet: 1.391 ± 0.26
2.466SerAsn: 2.466 ± 0.428
2.972SerPro: 2.972 ± 0.468
1.897SerGln: 1.897 ± 0.263
3.099SerArg: 3.099 ± 0.318
3.542SerSer: 3.542 ± 0.723
2.846SerThr: 2.846 ± 0.444
3.795SerVal: 3.795 ± 0.498
1.328SerTrp: 1.328 ± 0.353
1.518SerTyr: 1.518 ± 0.352
0.0SerXaa: 0.0 ± 0.0
Thr
5.755ThrAla: 5.755 ± 0.621
0.379ThrCys: 0.379 ± 0.183
4.301ThrAsp: 4.301 ± 0.517
4.49ThrGlu: 4.49 ± 0.552
2.277ThrPhe: 2.277 ± 0.391
6.261ThrGly: 6.261 ± 0.643
1.138ThrHis: 1.138 ± 0.34
2.593ThrIle: 2.593 ± 0.538
2.593ThrLys: 2.593 ± 0.316
5.502ThrLeu: 5.502 ± 0.697
0.759ThrMet: 0.759 ± 0.188
1.644ThrAsn: 1.644 ± 0.365
3.605ThrPro: 3.605 ± 0.42
1.708ThrGln: 1.708 ± 0.297
3.731ThrArg: 3.731 ± 0.476
3.668ThrSer: 3.668 ± 0.577
4.49ThrThr: 4.49 ± 0.578
4.87ThrVal: 4.87 ± 0.563
1.202ThrTrp: 1.202 ± 0.278
1.834ThrTyr: 1.834 ± 0.369
0.0ThrXaa: 0.0 ± 0.0
Val
7.083ValAla: 7.083 ± 0.703
0.443ValCys: 0.443 ± 0.158
4.806ValAsp: 4.806 ± 0.53
4.554ValGlu: 4.554 ± 0.531
2.277ValPhe: 2.277 ± 0.376
5.312ValGly: 5.312 ± 0.66
1.328ValHis: 1.328 ± 0.256
3.668ValIle: 3.668 ± 0.538
2.783ValLys: 2.783 ± 0.359
5.059ValLeu: 5.059 ± 0.557
1.012ValMet: 1.012 ± 0.317
2.34ValAsn: 2.34 ± 0.334
4.237ValPro: 4.237 ± 0.492
1.961ValGln: 1.961 ± 0.424
5.312ValArg: 5.312 ± 0.683
4.49ValSer: 4.49 ± 0.491
5.629ValThr: 5.629 ± 0.619
4.933ValVal: 4.933 ± 0.609
1.265ValTrp: 1.265 ± 0.282
2.277ValTyr: 2.277 ± 0.427
0.0ValXaa: 0.0 ± 0.0
Trp
1.455TrpAla: 1.455 ± 0.334
0.19TrpCys: 0.19 ± 0.109
1.328TrpAsp: 1.328 ± 0.274
1.391TrpGlu: 1.391 ± 0.275
1.012TrpPhe: 1.012 ± 0.244
1.644TrpGly: 1.644 ± 0.325
0.379TrpHis: 0.379 ± 0.155
1.138TrpIle: 1.138 ± 0.217
0.379TrpLys: 0.379 ± 0.176
1.961TrpLeu: 1.961 ± 0.328
0.379TrpMet: 0.379 ± 0.143
0.379TrpAsn: 0.379 ± 0.142
0.759TrpPro: 0.759 ± 0.274
0.696TrpGln: 0.696 ± 0.186
1.455TrpArg: 1.455 ± 0.291
0.885TrpSer: 0.885 ± 0.21
1.581TrpThr: 1.581 ± 0.351
2.087TrpVal: 2.087 ± 0.327
0.696TrpTrp: 0.696 ± 0.245
0.379TrpTyr: 0.379 ± 0.178
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.466TyrAla: 2.466 ± 0.41
0.316TyrCys: 0.316 ± 0.217
1.012TyrAsp: 1.012 ± 0.303
2.15TyrGlu: 2.15 ± 0.286
0.569TyrPhe: 0.569 ± 0.172
2.34TyrGly: 2.34 ± 0.418
0.632TyrHis: 0.632 ± 0.211
1.644TyrIle: 1.644 ± 0.384
1.138TyrLys: 1.138 ± 0.256
2.783TyrLeu: 2.783 ± 0.476
0.822TyrMet: 0.822 ± 0.234
1.075TyrAsn: 1.075 ± 0.249
1.581TyrPro: 1.581 ± 0.338
0.949TyrGln: 0.949 ± 0.218
2.53TyrArg: 2.53 ± 0.415
1.391TyrSer: 1.391 ± 0.257
2.087TyrThr: 2.087 ± 0.36
2.214TyrVal: 2.214 ± 0.331
0.443TyrTrp: 0.443 ± 0.165
0.696TyrTyr: 0.696 ± 0.21
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (15813 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski