Ciona intestinalis (Transparent sea squirt) (Ascidia intestinalis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Tunicata; Ascidiacea; Phlebobranchia; Cionidae; Ciona

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17309 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6Q0G0|F6Q0G0_CIOIN NTP_transferase domain-containing protein OS=Ciona intestinalis OX=7719 PE=4 SV=2
MM1 pKa = 8.18RR2 pKa = 11.84YY3 pKa = 9.65NCTVCSLIMATGLMHH18 pKa = 6.95SPIHH22 pKa = 5.85RR23 pKa = 11.84TMSPDD28 pKa = 3.15IAVTSYY34 pKa = 11.83DD35 pKa = 4.25DD36 pKa = 4.77GPVTSPTMRR45 pKa = 11.84SQVQDD50 pKa = 3.92FTSLAPPQTSKK61 pKa = 11.53YY62 pKa = 8.21DD63 pKa = 3.41TSDD66 pKa = 3.25GEE68 pKa = 4.17RR69 pKa = 11.84SDD71 pKa = 4.59RR72 pKa = 11.84YY73 pKa = 8.88YY74 pKa = 10.36TASSGDD80 pKa = 3.49EE81 pKa = 4.37NFDD84 pKa = 4.96DD85 pKa = 6.67DD86 pKa = 6.09YY87 pKa = 12.0DD88 pKa = 6.38DD89 pKa = 6.63DD90 pKa = 6.39DD91 pKa = 7.05DD92 pKa = 5.2EE93 pKa = 7.76DD94 pKa = 4.42GMCIRR99 pKa = 11.84LDD101 pKa = 3.7SDD103 pKa = 5.1DD104 pKa = 4.63DD105 pKa = 4.04TTSLSGLHH113 pKa = 6.54INLKK117 pKa = 8.35TLQAVSSMLAHH128 pKa = 6.02YY129 pKa = 10.14HH130 pKa = 6.8PIIQSDD136 pKa = 4.33PYY138 pKa = 10.65GNPIFVAPPPPEE150 pKa = 4.8DD151 pKa = 3.99EE152 pKa = 4.7YY153 pKa = 11.75SEE155 pKa = 4.06QDD157 pKa = 3.02EE158 pKa = 4.63AMASASGRR166 pKa = 11.84DD167 pKa = 3.84FLGSTTSHH175 pKa = 6.9DD176 pKa = 3.64TTLTGDD182 pKa = 4.57DD183 pKa = 5.11DD184 pKa = 4.44YY185 pKa = 12.1QCALQEE191 pKa = 4.37LDD193 pKa = 5.11DD194 pKa = 4.68VIIGQDD200 pKa = 3.21QTLLSDD206 pKa = 4.48ALTEE210 pKa = 4.19LTDD213 pKa = 3.45VMEE216 pKa = 4.22KK217 pKa = 7.9MTYY220 pKa = 10.37FSAEE224 pKa = 3.91AVDD227 pKa = 5.74DD228 pKa = 3.9II229 pKa = 5.23

Molecular weight: 25.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2XJJ3|H2XJJ3_CIOIN Uncharacterized protein OS=Ciona intestinalis OX=7719 PE=4 SV=1
MM1 pKa = 7.14VLGRR5 pKa = 11.84LVRR8 pKa = 11.84MALRR12 pKa = 11.84FVRR15 pKa = 11.84MALRR19 pKa = 11.84FVRR22 pKa = 11.84MALRR26 pKa = 11.84FVRR29 pKa = 11.84MALRR33 pKa = 11.84FVRR36 pKa = 11.84MALRR40 pKa = 11.84FVRR43 pKa = 11.84MALRR47 pKa = 11.84FVRR50 pKa = 11.84MALQFVRR57 pKa = 11.84MALQWIFNAWLL68 pKa = 3.47

Molecular weight: 8.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16678

631

17309

5768025

21

21338

333.2

37.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.869 ± 0.019

2.326 ± 0.023

5.347 ± 0.014

6.218 ± 0.026

4.091 ± 0.016

5.655 ± 0.026

2.535 ± 0.011

5.576 ± 0.015

6.471 ± 0.025

8.879 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.544 ± 0.01

5.156 ± 0.016

4.716 ± 0.021

4.182 ± 0.016

5.138 ± 0.02

8.155 ± 0.024

6.121 ± 0.02

6.642 ± 0.02

1.191 ± 0.008

3.187 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski