Thrips-associated genomovirus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus echi1

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8YT72|A0A1P8YT72_9VIRU RepA OS=Thrips-associated genomovirus 3 OX=1941237 PE=3 SV=1
MM1 pKa = 7.97PFRR4 pKa = 11.84FAAKK8 pKa = 10.01YY9 pKa = 10.66GLLTYY14 pKa = 7.2PQCGDD19 pKa = 3.72LDD21 pKa = 3.7PWSVSDD27 pKa = 3.44HH28 pKa = 6.45LGRR31 pKa = 11.84LGAEE35 pKa = 4.22CIIGRR40 pKa = 11.84EE41 pKa = 4.15DD42 pKa = 3.37HH43 pKa = 7.1SDD45 pKa = 3.54GGVHH49 pKa = 6.1LHH51 pKa = 6.35AFFMFEE57 pKa = 3.8RR58 pKa = 11.84RR59 pKa = 11.84FEE61 pKa = 4.22SRR63 pKa = 11.84DD64 pKa = 3.13VRR66 pKa = 11.84VFDD69 pKa = 4.26VEE71 pKa = 4.3GRR73 pKa = 11.84HH74 pKa = 5.7PNVVRR79 pKa = 11.84GYY81 pKa = 7.64STPSKK86 pKa = 10.33GYY88 pKa = 10.77AYY90 pKa = 8.25ATKK93 pKa = 10.63DD94 pKa = 3.16GDD96 pKa = 4.0VVAGGLEE103 pKa = 4.13CPIDD107 pKa = 3.88GASVSEE113 pKa = 5.0ASSKK117 pKa = 7.87WARR120 pKa = 11.84AILAEE125 pKa = 4.02SRR127 pKa = 11.84EE128 pKa = 4.04EE129 pKa = 3.76FFAIVAEE136 pKa = 4.23LDD138 pKa = 3.53PRR140 pKa = 11.84ALCCSFGSLRR150 pKa = 11.84AYY152 pKa = 10.47ADD154 pKa = 2.98WKK156 pKa = 9.94YY157 pKa = 11.07RR158 pKa = 11.84PVLDD162 pKa = 4.7QYY164 pKa = 8.68VTPAGISFCTDD175 pKa = 3.18EE176 pKa = 5.02VPQLHH181 pKa = 7.17DD182 pKa = 3.09WVQRR186 pKa = 11.84NLAGSVVGEE195 pKa = 4.12

Molecular weight: 21.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8YT72|A0A1P8YT72_9VIRU RepA OS=Thrips-associated genomovirus 3 OX=1941237 PE=3 SV=1
MM1 pKa = 7.0YY2 pKa = 10.13RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84PSKK8 pKa = 10.19RR9 pKa = 11.84RR10 pKa = 11.84SGGSRR15 pKa = 11.84RR16 pKa = 11.84TPRR19 pKa = 11.84SSRR22 pKa = 11.84RR23 pKa = 11.84SYY25 pKa = 11.22AKK27 pKa = 8.42KK28 pKa = 8.0TSYY31 pKa = 10.58RR32 pKa = 11.84RR33 pKa = 11.84TRR35 pKa = 11.84TVRR38 pKa = 11.84KK39 pKa = 7.28LTSRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84LLNITSQKK53 pKa = 10.32KK54 pKa = 8.32RR55 pKa = 11.84DD56 pKa = 3.45NMMPVTTDD64 pKa = 2.6WTGQTPIPGPAVLNGAGRR82 pKa = 11.84STILWCATQRR92 pKa = 11.84DD93 pKa = 4.41RR94 pKa = 11.84ASTPYY99 pKa = 10.06IDD101 pKa = 3.8NSEE104 pKa = 4.12AARR107 pKa = 11.84TSKK110 pKa = 10.79SIFLRR115 pKa = 11.84GVRR118 pKa = 11.84EE119 pKa = 3.9FASIRR124 pKa = 11.84TNSPDD129 pKa = 2.51AWRR132 pKa = 11.84WRR134 pKa = 11.84RR135 pKa = 11.84IIFSVKK141 pKa = 10.3GIFVYY146 pKa = 10.74LPLEE150 pKa = 3.84SRR152 pKa = 11.84FAVEE156 pKa = 4.2TSNQGWSRR164 pKa = 11.84AIPDD168 pKa = 3.64YY169 pKa = 11.02SGSASTTARR178 pKa = 11.84NALEE182 pKa = 3.81ALIFAGEE189 pKa = 4.07AGKK192 pKa = 10.27DD193 pKa = 3.12WASVFTAKK201 pKa = 9.94VDD203 pKa = 3.44HH204 pKa = 6.81SRR206 pKa = 11.84ITPLFDD212 pKa = 2.81KK213 pKa = 10.84TRR215 pKa = 11.84ILRR218 pKa = 11.84SGNQQGSYY226 pKa = 10.28FKK228 pKa = 11.06NKK230 pKa = 9.33LWLPVNKK237 pKa = 9.09TLLYY241 pKa = 10.68ADD243 pKa = 4.41EE244 pKa = 4.51EE245 pKa = 4.79SGNNVQDD252 pKa = 3.95GSLSAISRR260 pKa = 11.84QGVGDD265 pKa = 3.53IFIFDD270 pKa = 5.04LFDD273 pKa = 5.17CSTQNSASSLTFEE286 pKa = 5.37PEE288 pKa = 3.28ATMYY292 pKa = 8.49WHH294 pKa = 6.85EE295 pKa = 4.17RR296 pKa = 3.48

Molecular weight: 33.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

809

195

318

269.7

30.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.776 ± 0.443

2.101 ± 0.64

6.799 ± 0.984

4.944 ± 0.581

5.315 ± 0.45

8.158 ± 0.965

2.225 ± 0.714

4.326 ± 0.484

3.832 ± 0.426

7.417 ± 0.609

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.236 ± 0.063

2.472 ± 0.854

4.574 ± 0.161

2.596 ± 0.202

8.9 ± 1.58

8.776 ± 1.483

5.068 ± 1.505

6.18 ± 0.958

2.472 ± 0.126

3.832 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski