Methylophaga frappieri (strain ATCC BAA-2434 / DSM 25690 / JAM7)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Methylophaga

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2689 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1YKN2|I1YKN2_METFJ Flagellar secretion chaperone FliS OS=Methylophaga frappieri (strain ATCC BAA-2434 / DSM 25690 / JAM7) OX=754477 GN=Q7C_2341 PE=3 SV=1
MM1 pKa = 7.21VATTEE6 pKa = 4.2DD7 pKa = 3.68YY8 pKa = 10.73RR9 pKa = 11.84LSVYY13 pKa = 10.4QGADD17 pKa = 3.24DD18 pKa = 3.78ARR20 pKa = 11.84YY21 pKa = 9.93SGVVKK26 pKa = 10.43IFAEE30 pKa = 5.21GIVGSGSLLYY40 pKa = 10.13GGQMILTAAHH50 pKa = 7.12LFDD53 pKa = 5.95ANPDD57 pKa = 3.68SVEE60 pKa = 3.84VRR62 pKa = 11.84FDD64 pKa = 3.55APFGSSTTISASLLTILPAYY84 pKa = 10.43DD85 pKa = 3.78SVSQNYY91 pKa = 9.37DD92 pKa = 3.03VALLTLAQPAPDD104 pKa = 3.43YY105 pKa = 11.09ARR107 pKa = 11.84RR108 pKa = 11.84YY109 pKa = 9.69DD110 pKa = 4.07LYY112 pKa = 10.14RR113 pKa = 11.84QRR115 pKa = 11.84DD116 pKa = 3.73EE117 pKa = 3.88LHH119 pKa = 6.04QVFDD123 pKa = 3.78LVGYY127 pKa = 8.27GRR129 pKa = 11.84PGAGAEE135 pKa = 4.35GVTSEE140 pKa = 4.61VPTSAEE146 pKa = 3.4RR147 pKa = 11.84LVAQNRR153 pKa = 11.84FDD155 pKa = 4.01TDD157 pKa = 3.04VGTLDD162 pKa = 5.61LFTFDD167 pKa = 3.36QMSWGPVAGTQLVADD182 pKa = 4.81FDD184 pKa = 4.82DD185 pKa = 5.57GSVALDD191 pKa = 3.41SLGQLMGVNDD201 pKa = 4.82LGQNLLEE208 pKa = 4.28GLITPGDD215 pKa = 3.66SGGPAFIDD223 pKa = 3.92GQVAGVASYY232 pKa = 10.49IARR235 pKa = 11.84FAQGDD240 pKa = 3.68QYY242 pKa = 11.81LDD244 pKa = 3.45TNEE247 pKa = 4.91TIDD250 pKa = 3.75SSFGEE255 pKa = 3.9LAFWQRR261 pKa = 11.84VSSYY265 pKa = 7.07TQWIDD270 pKa = 3.24TQIQQTYY277 pKa = 9.18PDD279 pKa = 4.38APQHH283 pKa = 5.82PEE285 pKa = 3.8EE286 pKa = 4.6VVTAVLEE293 pKa = 4.23GDD295 pKa = 3.54PGSVQRR301 pKa = 11.84AYY303 pKa = 10.94FLLQFTGVVAPGEE316 pKa = 4.57SVSVAYY322 pKa = 7.43QTRR325 pKa = 11.84DD326 pKa = 3.06GTALAGEE333 pKa = 4.92DD334 pKa = 4.09YY335 pKa = 10.88IAVAGRR341 pKa = 11.84AVIYY345 pKa = 10.01GSQQQTVIAVEE356 pKa = 4.51LIEE359 pKa = 5.66DD360 pKa = 3.6VTAEE364 pKa = 4.24SNEE367 pKa = 4.11HH368 pKa = 5.57FWLDD372 pKa = 3.03ISDD375 pKa = 4.0PQGGVLPGGASVISAQRR392 pKa = 11.84LILDD396 pKa = 4.59DD397 pKa = 4.66DD398 pKa = 3.85QLIAA402 pKa = 5.14

Molecular weight: 43.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1YJA1|I1YJA1_METFJ Chromosomal replication initiator protein DnaA OS=Methylophaga frappieri (strain ATCC BAA-2434 / DSM 25690 / JAM7) OX=754477 GN=dnaA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 6.05GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.28TVGGRR28 pKa = 11.84RR29 pKa = 11.84VISARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.31GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2689

0

2689

835832

37

4182

310.8

34.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.594 ± 0.058

0.9 ± 0.017

5.904 ± 0.048

5.813 ± 0.048

3.823 ± 0.033

6.925 ± 0.06

2.339 ± 0.026

6.037 ± 0.036

4.091 ± 0.045

10.745 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.539 ± 0.025

3.796 ± 0.034

4.296 ± 0.03

5.269 ± 0.049

5.501 ± 0.044

6.084 ± 0.042

5.538 ± 0.049

6.709 ± 0.043

1.329 ± 0.021

2.767 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski