Beihai tombus-like virus 11

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFR2|A0A1L3KFR2_9VIRU RNA-directed RNA polymerase OS=Beihai tombus-like virus 11 OX=1922714 PE=4 SV=1
MM1 pKa = 7.49VSACQPCTDD10 pKa = 4.01NGAQYY15 pKa = 9.11VQEE18 pKa = 4.35VIAPSGATSCSGPPDD33 pKa = 3.88GCDD36 pKa = 3.1QSATKK41 pKa = 10.63GKK43 pKa = 8.32TRR45 pKa = 11.84DD46 pKa = 3.37QFVLSRR52 pKa = 11.84PDD54 pKa = 3.58FLPEE58 pKa = 3.82EE59 pKa = 4.37AWDD62 pKa = 3.65MVAFLTPYY70 pKa = 10.06YY71 pKa = 9.72VQQLVIVAFPSTVTPTQVRR90 pKa = 11.84TFLRR94 pKa = 11.84YY95 pKa = 9.03ILNSIPSEE103 pKa = 3.63EE104 pKa = 4.24WMGKK108 pKa = 7.55QLPGWHH114 pKa = 6.37NPADD118 pKa = 3.63YY119 pKa = 10.76SIYY122 pKa = 10.33DD123 pKa = 3.53DD124 pKa = 4.25NGNVVAGVPTMGVGWHH140 pKa = 6.5CPRR143 pKa = 11.84LLRR146 pKa = 11.84NLFPDD151 pKa = 4.02FGKK154 pKa = 10.26NPRR157 pKa = 11.84FVEE160 pKa = 4.24LVRR163 pKa = 11.84EE164 pKa = 4.04ARR166 pKa = 11.84RR167 pKa = 11.84WGRR170 pKa = 11.84YY171 pKa = 7.12LTITPVANATQFKK184 pKa = 10.43GRR186 pKa = 11.84IASASLQFNSALNPYY201 pKa = 9.73SLAITAGLSGPDD213 pKa = 4.13PLPPAISFPIGFVSLTLSLQLSRR236 pKa = 11.84DD237 pKa = 3.09RR238 pKa = 11.84LFYY241 pKa = 10.5YY242 pKa = 10.57DD243 pKa = 4.24GIRR246 pKa = 11.84WHH248 pKa = 6.69NIPWLNNPTEE258 pKa = 4.26VQFQSYY264 pKa = 10.49YY265 pKa = 11.14GFSIWNEE272 pKa = 3.79TGTDD276 pKa = 3.06ILVNSQAVITVRR288 pKa = 11.84ITITLTGVATVSWFVGPQLVHH309 pKa = 6.3QFTGDD314 pKa = 3.23VDD316 pKa = 3.47IGDD319 pKa = 4.29PLDD322 pKa = 3.57NQFLRR327 pKa = 11.84KK328 pKa = 9.45RR329 pKa = 11.84EE330 pKa = 3.79FDD332 pKa = 3.68SPSAAVSNEE341 pKa = 3.29LARR344 pKa = 11.84YY345 pKa = 8.94SVTPEE350 pKa = 3.75FSFEE354 pKa = 4.08ALLQADD360 pKa = 3.95EE361 pKa = 4.44KK362 pKa = 11.3SYY364 pKa = 10.17VNNWIEE370 pKa = 4.29GAHH373 pKa = 5.89SRR375 pKa = 11.84QAYY378 pKa = 9.71GKK380 pKa = 9.54DD381 pKa = 2.92HH382 pKa = 7.41LDD384 pKa = 3.51FVSTRR389 pKa = 11.84EE390 pKa = 3.85WRR392 pKa = 11.84PLIRR396 pKa = 11.84SANNVEE402 pKa = 4.4TVYY405 pKa = 11.32LDD407 pKa = 3.55ALSVKK412 pKa = 10.08RR413 pKa = 11.84DD414 pKa = 3.49LVDD417 pKa = 5.99LSGKK421 pKa = 7.21WQIDD425 pKa = 3.61YY426 pKa = 8.06ATNLDD431 pKa = 4.13PASSAAIVYY440 pKa = 7.04GTHH443 pKa = 5.88YY444 pKa = 10.08QFCCEE449 pKa = 3.98TSSEE453 pKa = 3.93FMLFKK458 pKa = 10.78RR459 pKa = 11.84EE460 pKa = 4.0VPEE463 pKa = 4.31KK464 pKa = 10.92DD465 pKa = 2.79EE466 pKa = 4.51GAIEE470 pKa = 4.03MAQVLQTALPHH481 pKa = 6.15TYY483 pKa = 9.04PAKK486 pKa = 10.69FNDD489 pKa = 3.97GGILPLILSKK499 pKa = 10.7VKK501 pKa = 10.49RR502 pKa = 11.84VGTNGLVGVAKK513 pKa = 10.78GIVNEE518 pKa = 3.91LLGGIRR524 pKa = 11.84EE525 pKa = 4.27IGTADD530 pKa = 4.06RR531 pKa = 11.84LIGYY535 pKa = 8.9PNMSTMYY542 pKa = 10.46GNNAVALYY550 pKa = 10.27NSNGNGNSNGNGNGKK565 pKa = 9.5KK566 pKa = 9.98KK567 pKa = 10.87KK568 pKa = 9.64NGNGNGVIPSIFSRR582 pKa = 11.84TMNII586 pKa = 3.26

Molecular weight: 64.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFI3|A0A1L3KFI3_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 11 OX=1922714 PE=4 SV=1
MM1 pKa = 7.22SRR3 pKa = 11.84SRR5 pKa = 11.84DD6 pKa = 3.59HH7 pKa = 6.78VPRR10 pKa = 11.84NLTEE14 pKa = 3.94VVEE17 pKa = 5.52FYY19 pKa = 10.79PHH21 pKa = 7.08LRR23 pKa = 11.84KK24 pKa = 9.58QVDD27 pKa = 3.18ALFRR31 pKa = 11.84KK32 pKa = 9.58ARR34 pKa = 11.84LADD37 pKa = 3.98GQPKK41 pKa = 9.65PKK43 pKa = 9.56PSKK46 pKa = 10.17KK47 pKa = 9.32PAPKK51 pKa = 9.97VVRR54 pKa = 11.84CLWCNSKK61 pKa = 10.56GYY63 pKa = 8.34RR64 pKa = 11.84TYY66 pKa = 11.66GDD68 pKa = 4.63DD69 pKa = 4.01DD70 pKa = 4.6LGYY73 pKa = 9.29TIADD77 pKa = 3.81CFRR80 pKa = 11.84CNTIFTFKK88 pKa = 10.55EE89 pKa = 3.75EE90 pKa = 3.86KK91 pKa = 11.03GEE93 pKa = 4.0IKK95 pKa = 10.44LRR97 pKa = 11.84SHH99 pKa = 7.35PFYY102 pKa = 10.94KK103 pKa = 10.25KK104 pKa = 10.12EE105 pKa = 4.11EE106 pKa = 4.11NVKK109 pKa = 10.44RR110 pKa = 11.84AVEE113 pKa = 4.22NIGKK117 pKa = 9.34EE118 pKa = 3.92VGNALGQIASKK129 pKa = 10.06SEE131 pKa = 4.01KK132 pKa = 8.48PNEE135 pKa = 3.74WVTVGKK141 pKa = 10.12NGKK144 pKa = 7.87PVKK147 pKa = 10.12KK148 pKa = 10.51DD149 pKa = 2.86ADD151 pKa = 3.79NKK153 pKa = 9.16VKK155 pKa = 10.53KK156 pKa = 9.96PKK158 pKa = 9.8VKK160 pKa = 10.57KK161 pKa = 9.88EE162 pKa = 3.62VSTKK166 pKa = 10.45VKK168 pKa = 10.45SPKK171 pKa = 9.72AKK173 pKa = 10.15KK174 pKa = 10.05DD175 pKa = 3.4VAVQASTPKK184 pKa = 10.29SKK186 pKa = 10.35KK187 pKa = 9.42GKK189 pKa = 8.47KK190 pKa = 9.75KK191 pKa = 10.14KK192 pKa = 10.54VKK194 pKa = 9.05TPKK197 pKa = 9.26GTNTPLIQRR206 pKa = 11.84KK207 pKa = 8.56AVTTDD212 pKa = 2.62MDD214 pKa = 3.69AVARR218 pKa = 11.84KK219 pKa = 9.61LKK221 pKa = 10.82AIAKK225 pKa = 9.25KK226 pKa = 10.48VNQEE230 pKa = 3.59RR231 pKa = 11.84SKK233 pKa = 10.79VQQSKK238 pKa = 10.33PKK240 pKa = 10.55AKK242 pKa = 7.65PTPPKK247 pKa = 10.38AVEE250 pKa = 4.11KK251 pKa = 9.64TVQEE255 pKa = 4.33PLPQRR260 pKa = 11.84VFPVVVQEE268 pKa = 4.07VLRR271 pKa = 11.84DD272 pKa = 3.81KK273 pKa = 10.69PIKK276 pKa = 9.95CQEE279 pKa = 3.89TEE281 pKa = 4.16EE282 pKa = 4.25IKK284 pKa = 11.0LVDD287 pKa = 3.8MPNINVDD294 pKa = 3.74RR295 pKa = 11.84MMKK298 pKa = 9.89TYY300 pKa = 8.31PTDD303 pKa = 3.36EE304 pKa = 5.15AEE306 pKa = 4.67FKK308 pKa = 11.24AMSANFRR315 pKa = 11.84QEE317 pKa = 4.63ALAQPAQAKK326 pKa = 9.19HH327 pKa = 4.72WLKK330 pKa = 10.82AADD333 pKa = 3.96RR334 pKa = 11.84LIYY337 pKa = 10.22SLPTRR342 pKa = 11.84VQVRR346 pKa = 11.84SPKK349 pKa = 10.6GLMRR353 pKa = 11.84AKK355 pKa = 10.23RR356 pKa = 11.84DD357 pKa = 3.92LITSRR362 pKa = 11.84PYY364 pKa = 8.86MHH366 pKa = 7.69GYY368 pKa = 9.2LQLCNQLDD376 pKa = 4.18DD377 pKa = 5.6FEE379 pKa = 4.94MKK381 pKa = 10.41HH382 pKa = 6.71SEE384 pKa = 4.12EE385 pKa = 5.58DD386 pKa = 3.55FMQAAAMQVALRR398 pKa = 11.84PLKK401 pKa = 10.64KK402 pKa = 10.08SWSKK406 pKa = 10.91SKK408 pKa = 10.69KK409 pKa = 9.54

Molecular weight: 46.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1466

409

586

488.7

55.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.094 ± 0.405

1.91 ± 0.512

5.184 ± 0.049

5.116 ± 0.442

3.752 ± 0.454

5.798 ± 1.197

1.842 ± 0.365

5.252 ± 0.859

8.254 ± 2.994

8.117 ± 0.825

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.456 ± 0.478

5.116 ± 0.764

6.003 ± 0.63

3.888 ± 0.463

6.003 ± 0.573

6.958 ± 0.624

4.638 ± 0.9

7.094 ± 0.793

1.501 ± 0.272

4.025 ± 0.747
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski