Amino acid dipepetide frequency for Streptococcus phage IPP43

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.01AlaAla: 3.01 ± 0.978
0.388AlaCys: 0.388 ± 0.199
4.758AlaAsp: 4.758 ± 0.618
5.437AlaGlu: 5.437 ± 0.964
2.136AlaPhe: 2.136 ± 0.432
3.398AlaGly: 3.398 ± 0.898
0.874AlaHis: 0.874 ± 0.246
5.34AlaIle: 5.34 ± 1.461
5.729AlaLys: 5.729 ± 0.63
6.894AlaLeu: 6.894 ± 1.029
1.942AlaMet: 1.942 ± 0.443
3.593AlaAsn: 3.593 ± 0.677
1.359AlaPro: 1.359 ± 0.369
3.495AlaGln: 3.495 ± 0.863
3.204AlaArg: 3.204 ± 0.383
4.078AlaSer: 4.078 ± 0.567
4.175AlaThr: 4.175 ± 0.78
3.593AlaVal: 3.593 ± 0.673
1.262AlaTrp: 1.262 ± 0.341
2.525AlaTyr: 2.525 ± 0.418
0.0AlaXaa: 0.0 ± 0.0
Cys
0.097CysAla: 0.097 ± 0.094
0.0CysCys: 0.0 ± 0.0
0.291CysAsp: 0.291 ± 0.182
0.874CysGlu: 0.874 ± 0.398
0.388CysPhe: 0.388 ± 0.178
0.291CysGly: 0.291 ± 0.178
0.291CysHis: 0.291 ± 0.173
0.194CysIle: 0.194 ± 0.147
0.291CysLys: 0.291 ± 0.166
0.971CysLeu: 0.971 ± 0.381
0.0CysMet: 0.0 ± 0.0
0.097CysAsn: 0.097 ± 0.111
0.097CysPro: 0.097 ± 0.117
0.0CysGln: 0.0 ± 0.0
0.388CysArg: 0.388 ± 0.207
0.485CysSer: 0.485 ± 0.289
0.194CysThr: 0.194 ± 0.13
0.291CysVal: 0.291 ± 0.165
0.0CysTrp: 0.0 ± 0.0
0.485CysTyr: 0.485 ± 0.203
0.0CysXaa: 0.0 ± 0.0
Asp
3.301AspAla: 3.301 ± 0.432
0.194AspCys: 0.194 ± 0.15
4.175AspAsp: 4.175 ± 0.772
3.69AspGlu: 3.69 ± 0.864
3.398AspPhe: 3.398 ± 0.745
4.952AspGly: 4.952 ± 0.706
1.359AspHis: 1.359 ± 0.427
4.952AspIle: 4.952 ± 0.66
4.952AspLys: 4.952 ± 0.887
4.661AspLeu: 4.661 ± 0.751
1.262AspMet: 1.262 ± 0.368
3.398AspAsn: 3.398 ± 0.492
1.554AspPro: 1.554 ± 0.445
1.554AspGln: 1.554 ± 0.336
1.942AspArg: 1.942 ± 0.361
3.107AspSer: 3.107 ± 0.505
3.301AspThr: 3.301 ± 0.577
4.078AspVal: 4.078 ± 0.719
0.874AspTrp: 0.874 ± 0.256
3.884AspTyr: 3.884 ± 0.683
0.0AspXaa: 0.0 ± 0.0
Glu
5.632GluAla: 5.632 ± 1.454
0.583GluCys: 0.583 ± 0.237
4.175GluAsp: 4.175 ± 0.64
7.574GluGlu: 7.574 ± 1.37
3.398GluPhe: 3.398 ± 0.529
2.525GluGly: 2.525 ± 0.451
0.485GluHis: 0.485 ± 0.191
6.505GluIle: 6.505 ± 0.822
8.933GluLys: 8.933 ± 1.426
9.127GluLeu: 9.127 ± 0.737
1.845GluMet: 1.845 ± 0.702
3.981GluAsn: 3.981 ± 0.731
2.039GluPro: 2.039 ± 0.643
3.301GluGln: 3.301 ± 0.785
3.495GluArg: 3.495 ± 0.741
4.078GluSer: 4.078 ± 0.432
3.981GluThr: 3.981 ± 0.533
4.369GluVal: 4.369 ± 0.591
1.456GluTrp: 1.456 ± 0.432
2.427GluTyr: 2.427 ± 0.471
0.0GluXaa: 0.0 ± 0.0
Phe
2.525PheAla: 2.525 ± 0.526
0.194PheCys: 0.194 ± 0.138
4.758PheAsp: 4.758 ± 0.731
3.107PheGlu: 3.107 ± 0.485
1.359PhePhe: 1.359 ± 0.488
1.942PheGly: 1.942 ± 0.449
0.388PheHis: 0.388 ± 0.172
3.107PheIle: 3.107 ± 0.556
3.593PheLys: 3.593 ± 0.661
2.427PheLeu: 2.427 ± 0.683
1.554PheMet: 1.554 ± 0.525
3.301PheAsn: 3.301 ± 0.545
0.68PhePro: 0.68 ± 0.242
1.262PheGln: 1.262 ± 0.345
1.068PheArg: 1.068 ± 0.333
2.816PheSer: 2.816 ± 0.563
3.204PheThr: 3.204 ± 0.438
1.359PheVal: 1.359 ± 0.304
0.583PheTrp: 0.583 ± 0.24
1.942PheTyr: 1.942 ± 0.432
0.0PheXaa: 0.0 ± 0.0
Gly
3.981GlyAla: 3.981 ± 1.02
0.194GlyCys: 0.194 ± 0.153
1.942GlyAsp: 1.942 ± 0.558
3.593GlyGlu: 3.593 ± 0.522
2.525GlyPhe: 2.525 ± 0.543
3.787GlyGly: 3.787 ± 0.668
0.68GlyHis: 0.68 ± 0.218
4.661GlyIle: 4.661 ± 0.534
4.661GlyLys: 4.661 ± 0.565
5.437GlyLeu: 5.437 ± 0.823
1.554GlyMet: 1.554 ± 0.522
3.01GlyAsn: 3.01 ± 0.44
0.68GlyPro: 0.68 ± 0.254
3.398GlyGln: 3.398 ± 0.691
2.816GlyArg: 2.816 ± 0.596
4.078GlySer: 4.078 ± 0.732
3.301GlyThr: 3.301 ± 0.548
3.787GlyVal: 3.787 ± 0.514
1.456GlyTrp: 1.456 ± 0.585
2.039GlyTyr: 2.039 ± 0.395
0.0GlyXaa: 0.0 ± 0.0
His
0.68HisAla: 0.68 ± 0.204
0.097HisCys: 0.097 ± 0.109
0.485HisAsp: 0.485 ± 0.206
1.359HisGlu: 1.359 ± 0.323
1.068HisPhe: 1.068 ± 0.264
0.874HisGly: 0.874 ± 0.336
0.291HisHis: 0.291 ± 0.186
1.651HisIle: 1.651 ± 0.345
0.777HisLys: 0.777 ± 0.275
1.359HisLeu: 1.359 ± 0.397
0.291HisMet: 0.291 ± 0.147
0.874HisAsn: 0.874 ± 0.242
0.388HisPro: 0.388 ± 0.18
0.485HisGln: 0.485 ± 0.291
0.485HisArg: 0.485 ± 0.298
1.165HisSer: 1.165 ± 0.475
0.874HisThr: 0.874 ± 0.361
0.971HisVal: 0.971 ± 0.268
0.097HisTrp: 0.097 ± 0.097
0.874HisTyr: 0.874 ± 0.269
0.0HisXaa: 0.0 ± 0.0
Ile
5.437IleAla: 5.437 ± 0.974
0.68IleCys: 0.68 ± 0.33
5.049IleAsp: 5.049 ± 0.74
6.7IleGlu: 6.7 ± 0.997
2.427IlePhe: 2.427 ± 0.655
3.107IleGly: 3.107 ± 0.719
1.165IleHis: 1.165 ± 0.348
3.398IleIle: 3.398 ± 0.712
5.146IleLys: 5.146 ± 0.779
4.952IleLeu: 4.952 ± 0.792
1.456IleMet: 1.456 ± 0.412
4.661IleAsn: 4.661 ± 0.583
1.845IlePro: 1.845 ± 0.352
2.719IleGln: 2.719 ± 0.401
3.495IleArg: 3.495 ± 0.79
5.826IleSer: 5.826 ± 1.056
4.175IleThr: 4.175 ± 0.487
3.398IleVal: 3.398 ± 0.514
1.068IleTrp: 1.068 ± 0.256
1.456IleTyr: 1.456 ± 0.442
0.0IleXaa: 0.0 ± 0.0
Lys
5.729LysAla: 5.729 ± 1.095
0.097LysCys: 0.097 ± 0.092
5.049LysAsp: 5.049 ± 0.653
7.282LysGlu: 7.282 ± 1.048
2.427LysPhe: 2.427 ± 0.42
4.661LysGly: 4.661 ± 0.687
1.748LysHis: 1.748 ± 0.321
4.466LysIle: 4.466 ± 0.685
5.729LysLys: 5.729 ± 1.004
6.797LysLeu: 6.797 ± 1.024
2.622LysMet: 2.622 ± 0.448
4.661LysAsn: 4.661 ± 0.551
1.748LysPro: 1.748 ± 0.408
4.175LysGln: 4.175 ± 0.6
4.272LysArg: 4.272 ± 0.615
5.923LysSer: 5.923 ± 0.572
5.826LysThr: 5.826 ± 0.533
4.466LysVal: 4.466 ± 0.573
0.971LysTrp: 0.971 ± 0.405
3.981LysTyr: 3.981 ± 0.692
0.0LysXaa: 0.0 ± 0.0
Leu
6.505LeuAla: 6.505 ± 0.997
0.583LeuCys: 0.583 ± 0.345
6.7LeuAsp: 6.7 ± 0.615
6.311LeuGlu: 6.311 ± 0.763
3.107LeuPhe: 3.107 ± 0.497
5.729LeuGly: 5.729 ± 0.734
1.456LeuHis: 1.456 ± 0.384
5.535LeuIle: 5.535 ± 0.54
7.962LeuLys: 7.962 ± 0.842
6.505LeuLeu: 6.505 ± 1.288
2.525LeuMet: 2.525 ± 0.784
5.826LeuAsn: 5.826 ± 0.708
2.719LeuPro: 2.719 ± 0.713
2.33LeuGln: 2.33 ± 0.592
4.369LeuArg: 4.369 ± 0.638
7.476LeuSer: 7.476 ± 0.872
4.758LeuThr: 4.758 ± 0.686
4.175LeuVal: 4.175 ± 0.584
0.583LeuTrp: 0.583 ± 0.365
2.525LeuTyr: 2.525 ± 0.436
0.0LeuXaa: 0.0 ± 0.0
Met
1.942MetAla: 1.942 ± 0.505
0.485MetCys: 0.485 ± 0.227
0.971MetAsp: 0.971 ± 0.396
2.427MetGlu: 2.427 ± 0.774
0.388MetPhe: 0.388 ± 0.222
1.359MetGly: 1.359 ± 0.297
0.097MetHis: 0.097 ± 0.134
1.456MetIle: 1.456 ± 0.54
2.525MetLys: 2.525 ± 0.661
2.136MetLeu: 2.136 ± 0.493
0.583MetMet: 0.583 ± 0.255
1.651MetAsn: 1.651 ± 0.574
0.68MetPro: 0.68 ± 0.279
0.583MetGln: 0.583 ± 0.237
0.874MetArg: 0.874 ± 0.37
1.651MetSer: 1.651 ± 0.547
1.845MetThr: 1.845 ± 0.48
1.942MetVal: 1.942 ± 0.395
0.097MetTrp: 0.097 ± 0.092
0.485MetTyr: 0.485 ± 0.225
0.0MetXaa: 0.0 ± 0.0
Asn
3.593AsnAla: 3.593 ± 0.611
0.097AsnCys: 0.097 ± 0.079
2.913AsnAsp: 2.913 ± 0.569
4.952AsnGlu: 4.952 ± 0.609
2.719AsnPhe: 2.719 ± 0.56
3.301AsnGly: 3.301 ± 0.817
1.165AsnHis: 1.165 ± 0.308
4.272AsnIle: 4.272 ± 0.638
3.981AsnLys: 3.981 ± 0.571
5.535AsnLeu: 5.535 ± 0.691
0.68AsnMet: 0.68 ± 0.39
2.913AsnAsn: 2.913 ± 0.627
2.816AsnPro: 2.816 ± 0.413
2.816AsnGln: 2.816 ± 0.75
3.107AsnArg: 3.107 ± 0.461
3.787AsnSer: 3.787 ± 0.703
3.204AsnThr: 3.204 ± 0.395
4.078AsnVal: 4.078 ± 0.476
1.068AsnTrp: 1.068 ± 0.24
1.651AsnTyr: 1.651 ± 0.345
0.0AsnXaa: 0.0 ± 0.0
Pro
2.233ProAla: 2.233 ± 0.395
0.291ProCys: 0.291 ± 0.187
2.233ProAsp: 2.233 ± 0.462
1.942ProGlu: 1.942 ± 0.578
0.68ProPhe: 0.68 ± 0.304
0.874ProGly: 0.874 ± 0.283
0.388ProHis: 0.388 ± 0.233
1.068ProIle: 1.068 ± 0.379
2.816ProLys: 2.816 ± 0.436
2.427ProLeu: 2.427 ± 0.685
0.388ProMet: 0.388 ± 0.199
0.874ProAsn: 0.874 ± 0.28
0.388ProPro: 0.388 ± 0.262
1.554ProGln: 1.554 ± 0.398
0.68ProArg: 0.68 ± 0.247
1.554ProSer: 1.554 ± 0.408
1.554ProThr: 1.554 ± 0.455
1.845ProVal: 1.845 ± 0.394
0.485ProTrp: 0.485 ± 0.179
0.68ProTyr: 0.68 ± 0.31
0.0ProXaa: 0.0 ± 0.0
Gln
3.593GlnAla: 3.593 ± 0.734
0.0GlnCys: 0.0 ± 0.0
1.845GlnAsp: 1.845 ± 0.383
4.175GlnGlu: 4.175 ± 0.875
1.456GlnPhe: 1.456 ± 0.327
2.233GlnGly: 2.233 ± 0.404
0.485GlnHis: 0.485 ± 0.216
2.719GlnIle: 2.719 ± 0.486
2.816GlnLys: 2.816 ± 0.516
3.69GlnLeu: 3.69 ± 0.469
0.874GlnMet: 0.874 ± 0.259
2.525GlnAsn: 2.525 ± 0.55
1.165GlnPro: 1.165 ± 0.342
1.456GlnGln: 1.456 ± 0.385
2.427GlnArg: 2.427 ± 0.527
2.913GlnSer: 2.913 ± 0.585
2.33GlnThr: 2.33 ± 0.703
2.913GlnVal: 2.913 ± 0.768
0.291GlnTrp: 0.291 ± 0.174
0.971GlnTyr: 0.971 ± 0.356
0.0GlnXaa: 0.0 ± 0.0
Arg
3.398ArgAla: 3.398 ± 0.511
0.583ArgCys: 0.583 ± 0.277
1.845ArgAsp: 1.845 ± 0.376
3.107ArgGlu: 3.107 ± 0.653
1.845ArgPhe: 1.845 ± 0.424
1.748ArgGly: 1.748 ± 0.301
0.777ArgHis: 0.777 ± 0.302
3.301ArgIle: 3.301 ± 0.832
4.272ArgLys: 4.272 ± 0.799
4.466ArgLeu: 4.466 ± 0.772
1.262ArgMet: 1.262 ± 0.323
3.301ArgAsn: 3.301 ± 0.776
1.554ArgPro: 1.554 ± 0.388
2.622ArgGln: 2.622 ± 0.597
2.136ArgArg: 2.136 ± 0.555
2.33ArgSer: 2.33 ± 0.348
2.913ArgThr: 2.913 ± 1.004
2.33ArgVal: 2.33 ± 0.492
0.68ArgTrp: 0.68 ± 0.182
2.136ArgTyr: 2.136 ± 0.559
0.0ArgXaa: 0.0 ± 0.0
Ser
4.855SerAla: 4.855 ± 0.959
0.194SerCys: 0.194 ± 0.137
3.593SerAsp: 3.593 ± 0.64
4.272SerGlu: 4.272 ± 0.679
3.204SerPhe: 3.204 ± 0.551
4.661SerGly: 4.661 ± 0.892
0.68SerHis: 0.68 ± 0.334
3.787SerIle: 3.787 ± 0.701
4.952SerLys: 4.952 ± 0.641
5.535SerLeu: 5.535 ± 0.846
0.971SerMet: 0.971 ± 0.406
4.272SerAsn: 4.272 ± 0.683
1.651SerPro: 1.651 ± 0.36
2.622SerGln: 2.622 ± 0.701
3.301SerArg: 3.301 ± 0.925
4.952SerSer: 4.952 ± 0.89
4.661SerThr: 4.661 ± 0.651
4.369SerVal: 4.369 ± 1.291
1.165SerTrp: 1.165 ± 0.353
2.33SerTyr: 2.33 ± 0.603
0.0SerXaa: 0.0 ± 0.0
Thr
4.466ThrAla: 4.466 ± 0.912
0.291ThrCys: 0.291 ± 0.183
4.078ThrAsp: 4.078 ± 0.627
3.398ThrGlu: 3.398 ± 0.515
3.593ThrPhe: 3.593 ± 0.699
5.049ThrGly: 5.049 ± 0.905
0.777ThrHis: 0.777 ± 0.24
5.632ThrIle: 5.632 ± 0.577
4.758ThrLys: 4.758 ± 0.418
5.049ThrLeu: 5.049 ± 0.68
1.262ThrMet: 1.262 ± 0.398
3.204ThrAsn: 3.204 ± 0.49
1.068ThrPro: 1.068 ± 0.377
1.845ThrGln: 1.845 ± 0.435
2.622ThrArg: 2.622 ± 0.629
3.981ThrSer: 3.981 ± 0.761
5.146ThrThr: 5.146 ± 1.075
3.495ThrVal: 3.495 ± 0.577
0.971ThrTrp: 0.971 ± 0.329
2.136ThrTyr: 2.136 ± 0.419
0.0ThrXaa: 0.0 ± 0.0
Val
3.301ValAla: 3.301 ± 0.743
0.485ValCys: 0.485 ± 0.21
3.593ValAsp: 3.593 ± 0.529
6.505ValGlu: 6.505 ± 0.631
2.427ValPhe: 2.427 ± 0.672
4.369ValGly: 4.369 ± 0.687
0.777ValHis: 0.777 ± 0.291
2.427ValIle: 2.427 ± 0.515
5.146ValLys: 5.146 ± 0.833
3.495ValLeu: 3.495 ± 0.514
1.456ValMet: 1.456 ± 0.363
3.301ValAsn: 3.301 ± 0.447
1.359ValPro: 1.359 ± 0.291
2.039ValGln: 2.039 ± 0.575
3.01ValArg: 3.01 ± 0.704
2.719ValSer: 2.719 ± 0.482
4.855ValThr: 4.855 ± 0.789
4.175ValVal: 4.175 ± 0.615
0.874ValTrp: 0.874 ± 0.315
1.651ValTyr: 1.651 ± 0.431
0.0ValXaa: 0.0 ± 0.0
Trp
0.777TrpAla: 0.777 ± 0.265
0.097TrpCys: 0.097 ± 0.092
0.291TrpAsp: 0.291 ± 0.146
0.874TrpGlu: 0.874 ± 0.26
0.68TrpPhe: 0.68 ± 0.315
0.971TrpGly: 0.971 ± 0.279
0.291TrpHis: 0.291 ± 0.192
1.359TrpIle: 1.359 ± 0.365
0.777TrpLys: 0.777 ± 0.301
1.748TrpLeu: 1.748 ± 0.455
0.583TrpMet: 0.583 ± 0.221
1.456TrpAsn: 1.456 ± 0.352
0.097TrpPro: 0.097 ± 0.094
0.388TrpGln: 0.388 ± 0.166
0.68TrpArg: 0.68 ± 0.253
1.165TrpSer: 1.165 ± 0.588
0.971TrpThr: 0.971 ± 0.235
0.68TrpVal: 0.68 ± 0.206
0.194TrpTrp: 0.194 ± 0.142
0.874TrpTyr: 0.874 ± 0.547
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.233TyrAla: 2.233 ± 0.382
0.194TyrCys: 0.194 ± 0.112
1.845TyrAsp: 1.845 ± 0.399
2.33TyrGlu: 2.33 ± 0.5
1.942TyrPhe: 1.942 ± 0.487
1.748TyrGly: 1.748 ± 0.407
0.971TyrHis: 0.971 ± 0.275
2.33TyrIle: 2.33 ± 0.475
2.816TyrLys: 2.816 ± 0.614
4.466TyrLeu: 4.466 ± 0.792
0.971TyrMet: 0.971 ± 0.395
1.748TyrAsn: 1.748 ± 0.586
1.068TyrPro: 1.068 ± 0.359
2.233TyrGln: 2.233 ± 0.626
2.233TyrArg: 2.233 ± 0.641
1.942TyrSer: 1.942 ± 0.349
1.554TyrThr: 1.554 ± 0.326
1.651TyrVal: 1.651 ± 0.337
0.777TyrTrp: 0.777 ± 0.262
2.136TyrTyr: 2.136 ± 0.799
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (10300 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski