Amino acid dipepetide frequency for Streptomyces phage SendItCS

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.085AlaAla: 10.085 ± 1.317
0.397AlaCys: 0.397 ± 0.213
4.816AlaAsp: 4.816 ± 0.665
6.402AlaGlu: 6.402 ± 0.512
2.55AlaPhe: 2.55 ± 0.37
7.819AlaGly: 7.819 ± 1.427
1.076AlaHis: 1.076 ± 0.264
4.929AlaIle: 4.929 ± 0.652
7.139AlaLys: 7.139 ± 0.713
7.649AlaLeu: 7.649 ± 1.388
3.229AlaMet: 3.229 ± 0.435
2.946AlaAsn: 2.946 ± 0.526
2.946AlaPro: 2.946 ± 0.478
3.173AlaGln: 3.173 ± 0.524
4.703AlaArg: 4.703 ± 0.628
4.986AlaSer: 4.986 ± 0.595
5.099AlaThr: 5.099 ± 0.443
7.025AlaVal: 7.025 ± 0.611
1.246AlaTrp: 1.246 ± 0.266
3.003AlaTyr: 3.003 ± 0.374
0.0AlaXaa: 0.0 ± 0.0
Cys
0.397CysAla: 0.397 ± 0.194
0.227CysCys: 0.227 ± 0.099
0.283CysAsp: 0.283 ± 0.133
0.227CysGlu: 0.227 ± 0.104
0.113CysPhe: 0.113 ± 0.082
0.397CysGly: 0.397 ± 0.168
0.113CysHis: 0.113 ± 0.082
0.567CysIle: 0.567 ± 0.178
0.227CysLys: 0.227 ± 0.141
0.51CysLeu: 0.51 ± 0.193
0.113CysMet: 0.113 ± 0.087
0.453CysAsn: 0.453 ± 0.177
0.283CysPro: 0.283 ± 0.136
0.057CysGln: 0.057 ± 0.071
0.057CysArg: 0.057 ± 0.057
0.85CysSer: 0.85 ± 0.246
0.34CysThr: 0.34 ± 0.168
0.397CysVal: 0.397 ± 0.176
0.17CysTrp: 0.17 ± 0.09
0.17CysTyr: 0.17 ± 0.102
0.0CysXaa: 0.0 ± 0.0
Asp
5.439AspAla: 5.439 ± 0.785
0.453AspCys: 0.453 ± 0.156
3.343AspAsp: 3.343 ± 0.626
5.892AspGlu: 5.892 ± 0.74
2.096AspPhe: 2.096 ± 0.351
5.722AspGly: 5.722 ± 0.724
0.963AspHis: 0.963 ± 0.251
2.833AspIle: 2.833 ± 0.459
2.606AspLys: 2.606 ± 0.345
4.589AspLeu: 4.589 ± 0.549
1.813AspMet: 1.813 ± 0.279
1.983AspAsn: 1.983 ± 0.4
3.796AspPro: 3.796 ± 0.609
2.436AspGln: 2.436 ± 0.362
3.286AspArg: 3.286 ± 0.568
3.909AspSer: 3.909 ± 0.531
3.796AspThr: 3.796 ± 0.439
3.909AspVal: 3.909 ± 0.46
0.907AspTrp: 0.907 ± 0.224
1.586AspTyr: 1.586 ± 0.305
0.0AspXaa: 0.0 ± 0.0
Glu
4.986GluAla: 4.986 ± 0.495
0.283GluCys: 0.283 ± 0.153
4.646GluAsp: 4.646 ± 0.619
6.289GluGlu: 6.289 ± 0.977
4.079GluPhe: 4.079 ± 0.526
4.249GluGly: 4.249 ± 0.506
1.473GluHis: 1.473 ± 0.321
4.363GluIle: 4.363 ± 0.641
4.873GluLys: 4.873 ± 0.562
6.006GluLeu: 6.006 ± 0.562
2.55GluMet: 2.55 ± 0.33
2.606GluAsn: 2.606 ± 0.404
2.776GluPro: 2.776 ± 0.486
2.04GluGln: 2.04 ± 0.347
4.419GluArg: 4.419 ± 0.541
3.456GluSer: 3.456 ± 0.48
4.703GluThr: 4.703 ± 0.614
4.193GluVal: 4.193 ± 0.569
1.076GluTrp: 1.076 ± 0.272
1.983GluTyr: 1.983 ± 0.299
0.0GluXaa: 0.0 ± 0.0
Phe
3.059PheAla: 3.059 ± 0.361
0.227PheCys: 0.227 ± 0.13
3.229PheAsp: 3.229 ± 0.461
2.436PheGlu: 2.436 ± 0.291
1.53PhePhe: 1.53 ± 0.281
3.003PheGly: 3.003 ± 0.563
0.737PheHis: 0.737 ± 0.211
2.096PheIle: 2.096 ± 0.345
2.21PheLys: 2.21 ± 0.32
2.833PheLeu: 2.833 ± 0.44
1.19PheMet: 1.19 ± 0.221
1.586PheAsn: 1.586 ± 0.37
1.133PhePro: 1.133 ± 0.212
1.643PheGln: 1.643 ± 0.288
1.926PheArg: 1.926 ± 0.353
2.153PheSer: 2.153 ± 0.408
2.72PheThr: 2.72 ± 0.489
2.663PheVal: 2.663 ± 0.42
0.567PheTrp: 0.567 ± 0.199
1.246PheTyr: 1.246 ± 0.322
0.0PheXaa: 0.0 ± 0.0
Gly
8.102GlyAla: 8.102 ± 0.829
0.453GlyCys: 0.453 ± 0.217
5.099GlyAsp: 5.099 ± 0.485
4.363GlyGlu: 4.363 ± 0.458
3.569GlyPhe: 3.569 ± 0.574
4.703GlyGly: 4.703 ± 0.783
1.36GlyHis: 1.36 ± 0.264
4.816GlyIle: 4.816 ± 0.86
3.966GlyLys: 3.966 ± 0.54
6.686GlyLeu: 6.686 ± 1.615
1.983GlyMet: 1.983 ± 0.292
2.38GlyAsn: 2.38 ± 0.351
2.89GlyPro: 2.89 ± 0.478
1.473GlyGln: 1.473 ± 0.324
3.456GlyArg: 3.456 ± 0.398
4.193GlySer: 4.193 ± 0.493
5.892GlyThr: 5.892 ± 0.547
6.402GlyVal: 6.402 ± 0.605
1.643GlyTrp: 1.643 ± 0.373
3.626GlyTyr: 3.626 ± 0.578
0.0GlyXaa: 0.0 ± 0.0
His
1.36HisAla: 1.36 ± 0.321
0.34HisCys: 0.34 ± 0.139
0.963HisAsp: 0.963 ± 0.244
0.737HisGlu: 0.737 ± 0.229
0.68HisPhe: 0.68 ± 0.202
1.643HisGly: 1.643 ± 0.331
0.453HisHis: 0.453 ± 0.146
0.963HisIle: 0.963 ± 0.247
0.68HisLys: 0.68 ± 0.188
1.246HisLeu: 1.246 ± 0.289
0.623HisMet: 0.623 ± 0.19
0.34HisAsn: 0.34 ± 0.155
0.907HisPro: 0.907 ± 0.246
0.51HisGln: 0.51 ± 0.197
0.907HisArg: 0.907 ± 0.245
1.53HisSer: 1.53 ± 0.297
0.68HisThr: 0.68 ± 0.186
1.303HisVal: 1.303 ± 0.284
0.227HisTrp: 0.227 ± 0.116
0.907HisTyr: 0.907 ± 0.224
0.0HisXaa: 0.0 ± 0.0
Ile
5.269IleAla: 5.269 ± 0.578
0.17IleCys: 0.17 ± 0.125
3.569IleAsp: 3.569 ± 0.355
4.363IleGlu: 4.363 ± 0.557
2.72IlePhe: 2.72 ± 0.416
4.873IleGly: 4.873 ± 0.871
0.963IleHis: 0.963 ± 0.274
1.983IleIle: 1.983 ± 0.425
3.286IleLys: 3.286 ± 0.432
4.533IleLeu: 4.533 ± 0.512
1.133IleMet: 1.133 ± 0.222
1.7IleAsn: 1.7 ± 0.276
2.493IlePro: 2.493 ± 0.366
1.7IleGln: 1.7 ± 0.366
2.72IleArg: 2.72 ± 0.384
3.173IleSer: 3.173 ± 0.451
4.079IleThr: 4.079 ± 0.555
3.683IleVal: 3.683 ± 0.463
0.68IleTrp: 0.68 ± 0.227
1.416IleTyr: 1.416 ± 0.29
0.0IleXaa: 0.0 ± 0.0
Lys
6.402LysAla: 6.402 ± 0.793
0.17LysCys: 0.17 ± 0.094
2.946LysAsp: 2.946 ± 0.477
4.136LysGlu: 4.136 ± 0.537
1.586LysPhe: 1.586 ± 0.306
4.136LysGly: 4.136 ± 0.604
0.963LysHis: 0.963 ± 0.292
3.796LysIle: 3.796 ± 0.384
4.929LysLys: 4.929 ± 0.763
5.666LysLeu: 5.666 ± 0.679
2.04LysMet: 2.04 ± 0.314
3.513LysAsn: 3.513 ± 0.431
3.229LysPro: 3.229 ± 0.454
1.7LysGln: 1.7 ± 0.277
3.286LysArg: 3.286 ± 0.547
4.079LysSer: 4.079 ± 0.594
4.136LysThr: 4.136 ± 0.668
4.703LysVal: 4.703 ± 0.5
0.907LysTrp: 0.907 ± 0.257
2.096LysTyr: 2.096 ± 0.361
0.0LysXaa: 0.0 ± 0.0
Leu
8.159LeuAla: 8.159 ± 1.257
0.227LeuCys: 0.227 ± 0.107
4.759LeuAsp: 4.759 ± 0.546
6.062LeuGlu: 6.062 ± 0.711
2.89LeuPhe: 2.89 ± 0.427
6.969LeuGly: 6.969 ± 1.219
1.02LeuHis: 1.02 ± 0.274
4.589LeuIle: 4.589 ± 0.471
5.666LeuLys: 5.666 ± 0.967
5.212LeuLeu: 5.212 ± 0.802
2.266LeuMet: 2.266 ± 0.374
3.853LeuAsn: 3.853 ± 0.445
2.89LeuPro: 2.89 ± 0.34
3.003LeuGln: 3.003 ± 0.428
4.079LeuArg: 4.079 ± 0.456
5.156LeuSer: 5.156 ± 0.755
5.666LeuThr: 5.666 ± 0.774
4.476LeuVal: 4.476 ± 0.551
0.85LeuTrp: 0.85 ± 0.211
2.606LeuTyr: 2.606 ± 0.41
0.0LeuXaa: 0.0 ± 0.0
Met
2.606MetAla: 2.606 ± 0.415
0.283MetCys: 0.283 ± 0.127
1.53MetAsp: 1.53 ± 0.322
2.096MetGlu: 2.096 ± 0.454
0.68MetPhe: 0.68 ± 0.239
1.586MetGly: 1.586 ± 0.304
0.453MetHis: 0.453 ± 0.167
1.416MetIle: 1.416 ± 0.294
1.813MetLys: 1.813 ± 0.333
2.153MetLeu: 2.153 ± 0.41
0.567MetMet: 0.567 ± 0.178
1.36MetAsn: 1.36 ± 0.301
1.416MetPro: 1.416 ± 0.289
0.68MetGln: 0.68 ± 0.179
1.246MetArg: 1.246 ± 0.293
2.153MetSer: 2.153 ± 0.341
1.926MetThr: 1.926 ± 0.311
1.473MetVal: 1.473 ± 0.301
0.227MetTrp: 0.227 ± 0.111
0.963MetTyr: 0.963 ± 0.195
0.0MetXaa: 0.0 ± 0.0
Asn
4.249AsnAla: 4.249 ± 0.465
0.283AsnCys: 0.283 ± 0.159
1.983AsnAsp: 1.983 ± 0.321
2.55AsnGlu: 2.55 ± 0.372
1.643AsnPhe: 1.643 ± 0.319
3.456AsnGly: 3.456 ± 0.499
0.737AsnHis: 0.737 ± 0.209
1.586AsnIle: 1.586 ± 0.262
2.72AsnLys: 2.72 ± 0.51
3.229AsnLeu: 3.229 ± 0.445
0.567AsnMet: 0.567 ± 0.213
1.586AsnAsn: 1.586 ± 0.284
2.38AsnPro: 2.38 ± 0.408
1.586AsnGln: 1.586 ± 0.302
2.21AsnArg: 2.21 ± 0.414
1.87AsnSer: 1.87 ± 0.438
1.983AsnThr: 1.983 ± 0.354
2.89AsnVal: 2.89 ± 0.36
0.567AsnTrp: 0.567 ± 0.187
1.473AsnTyr: 1.473 ± 0.304
0.0AsnXaa: 0.0 ± 0.0
Pro
3.173ProAla: 3.173 ± 0.513
0.453ProCys: 0.453 ± 0.195
3.399ProAsp: 3.399 ± 0.604
3.909ProGlu: 3.909 ± 0.539
1.36ProPhe: 1.36 ± 0.266
2.776ProGly: 2.776 ± 0.39
0.793ProHis: 0.793 ± 0.273
1.643ProIle: 1.643 ± 0.269
2.89ProLys: 2.89 ± 0.41
2.946ProLeu: 2.946 ± 0.466
1.076ProMet: 1.076 ± 0.243
2.153ProAsn: 2.153 ± 0.34
1.643ProPro: 1.643 ± 0.337
1.19ProGln: 1.19 ± 0.238
2.436ProArg: 2.436 ± 0.488
2.833ProSer: 2.833 ± 0.503
2.946ProThr: 2.946 ± 0.469
3.626ProVal: 3.626 ± 0.535
0.623ProTrp: 0.623 ± 0.277
1.246ProTyr: 1.246 ± 0.322
0.0ProXaa: 0.0 ± 0.0
Gln
3.003GlnAla: 3.003 ± 0.428
0.17GlnCys: 0.17 ± 0.095
1.87GlnAsp: 1.87 ± 0.313
1.473GlnGlu: 1.473 ± 0.305
1.36GlnPhe: 1.36 ± 0.28
2.38GlnGly: 2.38 ± 0.408
0.907GlnHis: 0.907 ± 0.227
2.153GlnIle: 2.153 ± 0.325
1.813GlnLys: 1.813 ± 0.34
2.55GlnLeu: 2.55 ± 0.517
0.907GlnMet: 0.907 ± 0.221
1.076GlnAsn: 1.076 ± 0.277
1.02GlnPro: 1.02 ± 0.237
1.756GlnGln: 1.756 ± 0.342
1.87GlnArg: 1.87 ± 0.353
1.756GlnSer: 1.756 ± 0.228
2.096GlnThr: 2.096 ± 0.405
2.04GlnVal: 2.04 ± 0.303
0.283GlnTrp: 0.283 ± 0.123
1.076GlnTyr: 1.076 ± 0.24
0.0GlnXaa: 0.0 ± 0.0
Arg
3.399ArgAla: 3.399 ± 0.526
0.397ArgCys: 0.397 ± 0.16
3.626ArgAsp: 3.626 ± 0.479
3.909ArgGlu: 3.909 ± 0.436
1.813ArgPhe: 1.813 ± 0.301
2.55ArgGly: 2.55 ± 0.455
0.793ArgHis: 0.793 ± 0.233
3.003ArgIle: 3.003 ± 0.573
4.249ArgLys: 4.249 ± 0.515
5.042ArgLeu: 5.042 ± 0.614
1.926ArgMet: 1.926 ± 0.305
2.153ArgAsn: 2.153 ± 0.342
2.436ArgPro: 2.436 ± 0.533
1.813ArgGln: 1.813 ± 0.346
4.079ArgArg: 4.079 ± 0.656
3.059ArgSer: 3.059 ± 0.478
3.343ArgThr: 3.343 ± 0.513
3.796ArgVal: 3.796 ± 0.536
0.567ArgTrp: 0.567 ± 0.217
1.643ArgTyr: 1.643 ± 0.383
0.0ArgXaa: 0.0 ± 0.0
Ser
5.156SerAla: 5.156 ± 0.785
0.17SerCys: 0.17 ± 0.104
3.683SerAsp: 3.683 ± 0.57
4.023SerGlu: 4.023 ± 0.513
2.153SerPhe: 2.153 ± 0.377
6.346SerGly: 6.346 ± 0.632
0.85SerHis: 0.85 ± 0.276
3.173SerIle: 3.173 ± 0.396
3.513SerLys: 3.513 ± 0.485
5.269SerLeu: 5.269 ± 0.607
1.19SerMet: 1.19 ± 0.253
1.813SerAsn: 1.813 ± 0.312
2.89SerPro: 2.89 ± 0.429
1.586SerGln: 1.586 ± 0.306
3.229SerArg: 3.229 ± 0.521
4.533SerSer: 4.533 ± 0.719
3.966SerThr: 3.966 ± 0.608
3.399SerVal: 3.399 ± 0.356
1.303SerTrp: 1.303 ± 0.217
1.643SerTyr: 1.643 ± 0.291
0.0SerXaa: 0.0 ± 0.0
Thr
5.892ThrAla: 5.892 ± 0.699
0.34ThrCys: 0.34 ± 0.207
3.739ThrAsp: 3.739 ± 0.463
4.136ThrGlu: 4.136 ± 0.511
2.833ThrPhe: 2.833 ± 0.431
5.836ThrGly: 5.836 ± 0.879
1.303ThrHis: 1.303 ± 0.295
4.193ThrIle: 4.193 ± 0.515
4.079ThrLys: 4.079 ± 0.533
5.439ThrLeu: 5.439 ± 0.523
0.907ThrMet: 0.907 ± 0.168
2.04ThrAsn: 2.04 ± 0.347
3.683ThrPro: 3.683 ± 0.5
2.21ThrGln: 2.21 ± 0.446
2.946ThrArg: 2.946 ± 0.388
2.946ThrSer: 2.946 ± 0.545
4.703ThrThr: 4.703 ± 0.916
5.779ThrVal: 5.779 ± 0.723
0.907ThrTrp: 0.907 ± 0.267
1.983ThrTyr: 1.983 ± 0.38
0.0ThrXaa: 0.0 ± 0.0
Val
5.949ValAla: 5.949 ± 0.538
0.51ValCys: 0.51 ± 0.189
4.249ValAsp: 4.249 ± 0.418
5.099ValGlu: 5.099 ± 0.629
2.493ValPhe: 2.493 ± 0.408
5.326ValGly: 5.326 ± 0.606
1.133ValHis: 1.133 ± 0.274
4.363ValIle: 4.363 ± 0.347
4.589ValLys: 4.589 ± 0.438
5.609ValLeu: 5.609 ± 0.589
1.643ValMet: 1.643 ± 0.317
2.833ValAsn: 2.833 ± 0.352
2.323ValPro: 2.323 ± 0.34
1.7ValGln: 1.7 ± 0.385
4.136ValArg: 4.136 ± 0.582
4.023ValSer: 4.023 ± 0.462
5.439ValThr: 5.439 ± 0.785
4.929ValVal: 4.929 ± 0.547
1.076ValTrp: 1.076 ± 0.266
2.89ValTyr: 2.89 ± 0.533
0.0ValXaa: 0.0 ± 0.0
Trp
1.303TrpAla: 1.303 ± 0.325
0.17TrpCys: 0.17 ± 0.121
1.076TrpAsp: 1.076 ± 0.288
1.076TrpGlu: 1.076 ± 0.206
0.623TrpPhe: 0.623 ± 0.209
0.963TrpGly: 0.963 ± 0.216
0.283TrpHis: 0.283 ± 0.114
0.68TrpIle: 0.68 ± 0.244
0.793TrpLys: 0.793 ± 0.236
0.963TrpLeu: 0.963 ± 0.236
0.283TrpMet: 0.283 ± 0.126
1.473TrpAsn: 1.473 ± 0.348
0.34TrpPro: 0.34 ± 0.129
0.17TrpGln: 0.17 ± 0.079
0.793TrpArg: 0.793 ± 0.248
1.246TrpSer: 1.246 ± 0.357
0.85TrpThr: 0.85 ± 0.307
1.133TrpVal: 1.133 ± 0.321
0.34TrpTrp: 0.34 ± 0.151
0.34TrpTyr: 0.34 ± 0.159
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.229TyrAla: 3.229 ± 0.418
0.227TyrCys: 0.227 ± 0.112
2.55TyrAsp: 2.55 ± 0.445
1.87TyrGlu: 1.87 ± 0.426
1.416TyrPhe: 1.416 ± 0.288
2.323TyrGly: 2.323 ± 0.375
0.623TyrHis: 0.623 ± 0.191
1.36TyrIle: 1.36 ± 0.319
2.38TyrLys: 2.38 ± 0.455
2.266TyrLeu: 2.266 ± 0.455
0.567TyrMet: 0.567 ± 0.204
1.7TyrAsn: 1.7 ± 0.312
1.643TyrPro: 1.643 ± 0.319
1.133TyrGln: 1.133 ± 0.244
1.813TyrArg: 1.813 ± 0.312
1.983TyrSer: 1.983 ± 0.28
1.53TyrThr: 1.53 ± 0.33
2.55TyrVal: 2.55 ± 0.338
0.737TyrTrp: 0.737 ± 0.236
1.53TyrTyr: 1.53 ± 0.333
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (17651 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski