Paenibacillus lentus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4362 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8SAX9|A0A3Q8SAX9_9BACL Crossover junction endodeoxyribonuclease RuvC OS=Paenibacillus lentus OX=1338368 GN=ruvC PE=3 SV=1
MM1 pKa = 7.66LSICFPDD8 pKa = 3.87LPGAITCGEE17 pKa = 4.04NDD19 pKa = 3.34EE20 pKa = 4.5EE21 pKa = 4.17ALYY24 pKa = 9.4MAEE27 pKa = 4.58DD28 pKa = 4.25CLALHH33 pKa = 7.0LYY35 pKa = 10.4GMEE38 pKa = 4.16EE39 pKa = 4.76DD40 pKa = 3.97GDD42 pKa = 4.46EE43 pKa = 4.27IPEE46 pKa = 4.08SSRR49 pKa = 11.84DD50 pKa = 3.5KK51 pKa = 11.41LKK53 pKa = 10.26TNKK56 pKa = 9.4PLFPIGSGLLVSS68 pKa = 4.64

Molecular weight: 7.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8S9P0|A0A3Q8S9P0_9BACL ABC transporter permease OS=Paenibacillus lentus OX=1338368 GN=EIM92_04815 PE=3 SV=1
MM1 pKa = 7.59KK2 pKa = 8.7PTFKK6 pKa = 10.81PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.2NGRR28 pKa = 11.84KK29 pKa = 9.23VIAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.16GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4362

0

4362

1430031

26

6330

327.8

36.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.711 ± 0.04

0.751 ± 0.009

5.155 ± 0.028

7.17 ± 0.043

4.105 ± 0.028

7.267 ± 0.037

2.123 ± 0.017

7.037 ± 0.039

5.472 ± 0.036

10.054 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.834 ± 0.017

3.998 ± 0.028

3.87 ± 0.026

4.034 ± 0.027

5.004 ± 0.04

6.389 ± 0.03

5.239 ± 0.026

6.92 ± 0.032

1.23 ± 0.016

3.639 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski