Escherichia phage slur05

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dhillonvirus; unclassified Dhillonvirus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M7QA91|A0A0M7QA91_9CAUD Uncharacterized protein OS=Escherichia phage slur05 OX=1720498 PE=4 SV=1
MM1 pKa = 7.56TPMQITLMAGYY12 pKa = 8.89SQEE15 pKa = 4.85FIDD18 pKa = 6.27LVSCYY23 pKa = 10.61DD24 pKa = 3.86DD25 pKa = 3.44YY26 pKa = 11.52THH28 pKa = 6.85YY29 pKa = 11.17GIATVCDD36 pKa = 4.13GDD38 pKa = 4.42NLTADD43 pKa = 4.27EE44 pKa = 4.86LDD46 pKa = 3.78EE47 pKa = 4.9VDD49 pKa = 5.94DD50 pKa = 3.93YY51 pKa = 10.28TTEE54 pKa = 4.01EE55 pKa = 4.64LCALMCGVPVWAKK68 pKa = 9.9EE69 pKa = 4.17SPLAADD75 pKa = 4.16GDD77 pKa = 3.64NMYY80 pKa = 10.46RR81 pKa = 11.84YY82 pKa = 10.01RR83 pKa = 11.84KK84 pKa = 9.6

Molecular weight: 9.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M7QA82|A0A0M7QA82_9CAUD Uncharacterized protein OS=Escherichia phage slur05 OX=1720498 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.55LLFRR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 9.31HH9 pKa = 6.35DD10 pKa = 3.56GRR12 pKa = 11.84ILKK15 pKa = 9.24PIQTLDD21 pKa = 3.27SHH23 pKa = 7.25VRR25 pKa = 11.84LQNAAGGRR33 pKa = 11.84VWWAKK38 pKa = 10.63KK39 pKa = 8.59YY40 pKa = 7.62TLPEE44 pKa = 4.06NFDD47 pKa = 3.75VLTHH51 pKa = 6.71DD52 pKa = 4.0IEE54 pKa = 6.28RR55 pKa = 11.84GDD57 pKa = 3.63VLRR60 pKa = 11.84DD61 pKa = 3.27IRR63 pKa = 11.84DD64 pKa = 3.28GGLWVVEE71 pKa = 3.7YY72 pKa = 10.63VGRR75 pKa = 11.84HH76 pKa = 4.7GLRR79 pKa = 11.84MQSRR83 pKa = 11.84KK84 pKa = 10.54DD85 pKa = 3.45GVVGNIQRR93 pKa = 11.84GMTYY97 pKa = 10.34FGLINYY103 pKa = 7.26EE104 pKa = 3.93RR105 pKa = 11.84VGRR108 pKa = 11.84KK109 pKa = 9.02YY110 pKa = 10.78SLRR113 pKa = 11.84DD114 pKa = 3.5RR115 pKa = 11.84QPHH118 pKa = 5.2EE119 pKa = 3.69RR120 pKa = 11.84VEE122 pKa = 4.29FRR124 pKa = 11.84KK125 pKa = 9.99SQPAYY130 pKa = 9.27ATYY133 pKa = 10.44DD134 pKa = 3.12ASKK137 pKa = 10.46VMEE140 pKa = 5.6PIRR143 pKa = 11.84QATRR147 pKa = 11.84EE148 pKa = 3.86IVNRR152 pKa = 11.84FDD154 pKa = 4.66AVTNAQRR161 pKa = 11.84QARR164 pKa = 11.84EE165 pKa = 3.84YY166 pKa = 8.58GVGFIRR172 pKa = 11.84VEE174 pKa = 3.78PDD176 pKa = 2.75GSMKK180 pKa = 10.73AIDD183 pKa = 3.98PRR185 pKa = 11.84SVIMKK190 pKa = 10.0

Molecular weight: 22.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

13452

37

1116

231.9

25.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.452 ± 0.548

1.123 ± 0.145

6.594 ± 0.302

6.178 ± 0.272

3.516 ± 0.174

7.806 ± 0.268

1.717 ± 0.17

5.427 ± 0.239

6.096 ± 0.4

7.382 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.706 ± 0.198

4.274 ± 0.254

3.97 ± 0.205

3.836 ± 0.251

6.178 ± 0.296

4.869 ± 0.269

5.962 ± 0.273

7.315 ± 0.298

1.561 ± 0.156

3.04 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski