Hesseltinella vesiculosa

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina; Mucoromycetes; Mucorales; Cunninghamellaceae; Hesseltinella

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11058 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X2GUL2|A0A1X2GUL2_9FUNG Phospholipid-transporting ATPase OS=Hesseltinella vesiculosa OX=101127 GN=DM01DRAFT_1280350 PE=3 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84GEE4 pKa = 5.03CMFFCYY10 pKa = 9.69QCQKK14 pKa = 10.89KK15 pKa = 10.19VGTIATSGADD25 pKa = 3.7TRR27 pKa = 11.84CSEE30 pKa = 4.28CLSVNIDD37 pKa = 3.71RR38 pKa = 11.84IGEE41 pKa = 4.12HH42 pKa = 6.61SDD44 pKa = 2.59WFYY47 pKa = 11.65DD48 pKa = 4.24EE49 pKa = 6.05SSQEE53 pKa = 4.0DD54 pKa = 4.03NDD56 pKa = 4.01RR57 pKa = 11.84DD58 pKa = 3.82DD59 pKa = 5.59YY60 pKa = 11.97NDD62 pKa = 4.0EE63 pKa = 4.13EE64 pKa = 4.97CEE66 pKa = 4.26SANEE70 pKa = 3.69QDD72 pKa = 3.7AAYY75 pKa = 10.72NDD77 pKa = 4.42LSHH80 pKa = 6.94LMSTVSVSSDD90 pKa = 2.85PGTDD94 pKa = 3.92LDD96 pKa = 3.98HH97 pKa = 7.15QEE99 pKa = 4.28NDD101 pKa = 4.48SPDD104 pKa = 3.62EE105 pKa = 4.98DD106 pKa = 4.77DD107 pKa = 5.95DD108 pKa = 5.43EE109 pKa = 4.9PVSDD113 pKa = 5.7DD114 pKa = 6.39NDD116 pKa = 4.21DD117 pKa = 3.09QWQDD121 pKa = 2.86MDD123 pKa = 4.81EE124 pKa = 4.73DD125 pKa = 4.93DD126 pKa = 5.57NLDD129 pKa = 3.48SDD131 pKa = 4.79EE132 pKa = 4.44EE133 pKa = 4.75DD134 pKa = 3.4EE135 pKa = 4.52AASPFHH141 pKa = 6.81LCPTHH146 pKa = 8.05ASMFAAAFSQAAQTATFHH164 pKa = 6.27TSDD167 pKa = 3.46PRR169 pKa = 11.84LRR171 pKa = 11.84LADD174 pKa = 4.06FMHH177 pKa = 7.17RR178 pKa = 11.84LYY180 pKa = 11.34SLDD183 pKa = 4.93DD184 pKa = 4.81DD185 pKa = 5.52DD186 pKa = 7.57DD187 pKa = 4.48YY188 pKa = 12.09FEE190 pKa = 6.51DD191 pKa = 5.19SDD193 pKa = 5.19YY194 pKa = 11.82DD195 pKa = 4.62DD196 pKa = 6.71DD197 pKa = 7.0DD198 pKa = 7.28DD199 pKa = 6.32EE200 pKa = 7.91DD201 pKa = 6.59DD202 pKa = 5.16DD203 pKa = 6.33DD204 pKa = 5.96EE205 pKa = 5.46EE206 pKa = 6.0EE207 pKa = 5.01EE208 pKa = 5.88DD209 pKa = 5.14DD210 pKa = 5.79DD211 pKa = 6.99DD212 pKa = 7.69DD213 pKa = 5.28IFADD217 pKa = 3.76EE218 pKa = 5.58DD219 pKa = 4.25GVSDD223 pKa = 5.47YY224 pKa = 11.97SNAWDD229 pKa = 3.55QNSNGDD235 pKa = 3.37SHH237 pKa = 8.04EE238 pKa = 5.28DD239 pKa = 3.35IEE241 pKa = 4.94EE242 pKa = 4.38LDD244 pKa = 4.62SLDD247 pKa = 4.39DD248 pKa = 5.29DD249 pKa = 4.33YY250 pKa = 12.11GEE252 pKa = 4.33MGNLGGFDD260 pKa = 3.82VDD262 pKa = 4.14TDD264 pKa = 5.35LLDD267 pKa = 4.21LLNNEE272 pKa = 5.02DD273 pKa = 4.33TDD275 pKa = 4.36DD276 pKa = 3.96MAWDD280 pKa = 3.84EE281 pKa = 4.52LAPSYY286 pKa = 10.72EE287 pKa = 4.23VLGTTLMADD296 pKa = 4.81RR297 pKa = 11.84SDD299 pKa = 3.77QDD301 pKa = 3.45DD302 pKa = 4.76GYY304 pKa = 10.62LGSVLEE310 pKa = 4.49VVHH313 pKa = 6.89RR314 pKa = 11.84EE315 pKa = 4.03YY316 pKa = 11.12EE317 pKa = 3.97PGPFEE322 pKa = 4.06EE323 pKa = 6.01HH324 pKa = 7.16PDD326 pKa = 3.66FLNGSIYY333 pKa = 10.64YY334 pKa = 9.69EE335 pKa = 4.1EE336 pKa = 4.13EE337 pKa = 4.12VVTRR341 pKa = 11.84RR342 pKa = 11.84HH343 pKa = 4.23QSRR346 pKa = 11.84FLRR349 pKa = 11.84LRR351 pKa = 11.84LRR353 pKa = 11.84HH354 pKa = 6.28IYY356 pKa = 9.06STMEE360 pKa = 4.06TNSEE364 pKa = 4.1AEE366 pKa = 4.37QPRR369 pKa = 11.84HH370 pKa = 5.84IPFEE374 pKa = 4.25NLSSRR379 pKa = 11.84TLHH382 pKa = 6.72DD383 pKa = 3.25QDD385 pKa = 4.44HH386 pKa = 6.61LVTASVDD393 pKa = 3.93CIICQEE399 pKa = 4.39TYY401 pKa = 8.08QTGKK405 pKa = 10.75LITKK409 pKa = 7.27MPCGHH414 pKa = 7.1EE415 pKa = 4.0YY416 pKa = 10.2HH417 pKa = 7.38DD418 pKa = 3.41EE419 pKa = 4.73CIRR422 pKa = 11.84HH423 pKa = 5.56WLNHH427 pKa = 6.54NITCPVCRR435 pKa = 11.84RR436 pKa = 11.84SLQEE440 pKa = 3.49VDD442 pKa = 3.86SVVSS446 pKa = 3.57

Molecular weight: 51.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X2GIR6|A0A1X2GIR6_9FUNG Elongation of fatty acids protein OS=Hesseltinella vesiculosa OX=101127 GN=DM01DRAFT_1407422 PE=3 SV=1
NN1 pKa = 6.58QKK3 pKa = 10.46SAVSQRR9 pKa = 11.84KK10 pKa = 8.66SAVSQRR16 pKa = 11.84KK17 pKa = 8.66SAVSQRR23 pKa = 11.84KK24 pKa = 8.5SAGANQKK31 pKa = 10.35SAVSQRR37 pKa = 11.84KK38 pKa = 8.66SAVSQRR44 pKa = 11.84KK45 pKa = 8.5SAGANQKK52 pKa = 7.84TSKK55 pKa = 10.34KK56 pKa = 10.26SAGANQKK63 pKa = 10.35SAVSQRR69 pKa = 11.84KK70 pKa = 8.66SAVSQRR76 pKa = 11.84KK77 pKa = 8.68SAVSQQKK84 pKa = 9.82NAVSQRR90 pKa = 11.84KK91 pKa = 8.48SAGANQKK98 pKa = 10.35SAVSQRR104 pKa = 11.84KK105 pKa = 8.68SAVSQQKK112 pKa = 9.75KK113 pKa = 9.88CRR115 pKa = 11.84SQSKK119 pKa = 9.41KK120 pKa = 10.11CRR122 pKa = 11.84SQRR125 pKa = 11.84KK126 pKa = 8.48SAVSQRR132 pKa = 11.84KK133 pKa = 8.68SAVSQQKK140 pKa = 9.95IPGKK144 pKa = 9.72FQQ146 pKa = 3.29

Molecular weight: 15.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11058

0

11058

4440516

49

6093

401.6

45.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.583 ± 0.022

1.475 ± 0.011

6.154 ± 0.022

5.115 ± 0.026

3.896 ± 0.017

5.158 ± 0.023

3.059 ± 0.016

5.002 ± 0.019

5.167 ± 0.025

9.848 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.01

3.915 ± 0.015

5.897 ± 0.03

5.096 ± 0.024

5.247 ± 0.019

8.069 ± 0.034

6.204 ± 0.021

6.12 ± 0.022

1.28 ± 0.009

3.082 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski