Avon-Heathcote Estuary associated circular virus 11

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IB17|A0A0C5IB17_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 11 OX=1618234 PE=3 SV=1
MM1 pKa = 7.46IAHH4 pKa = 6.14NRR6 pKa = 11.84AFFTISITGLKK17 pKa = 9.73GMCDD21 pKa = 2.88IIFFLFLSLAQMASPKK37 pKa = 10.0KK38 pKa = 7.67YY39 pKa = 10.04NRR41 pKa = 11.84MIFTINNPGPNRR53 pKa = 11.84PQFLEE58 pKa = 3.4QHH60 pKa = 5.2MAYY63 pKa = 9.29MIYY66 pKa = 10.37VLEE69 pKa = 5.07EE70 pKa = 4.13GDD72 pKa = 3.71QTHH75 pKa = 6.29TPHH78 pKa = 5.94LQGYY82 pKa = 8.62IRR84 pKa = 11.84WKK86 pKa = 9.74CRR88 pKa = 11.84KK89 pKa = 6.57TFKK92 pKa = 10.38SAEE95 pKa = 3.97RR96 pKa = 11.84LLGGRR101 pKa = 11.84AYY103 pKa = 11.05LEE105 pKa = 3.92IARR108 pKa = 11.84GTEE111 pKa = 4.05EE112 pKa = 4.71EE113 pKa = 3.99NQAYY117 pKa = 9.22CKK119 pKa = 10.41KK120 pKa = 10.32EE121 pKa = 3.96EE122 pKa = 4.35GVLEE126 pKa = 4.25PWQEE130 pKa = 3.59FGTYY134 pKa = 10.15DD135 pKa = 3.44GGSGIKK141 pKa = 10.05GRR143 pKa = 11.84RR144 pKa = 11.84TDD146 pKa = 3.66LEE148 pKa = 4.03AVAKK152 pKa = 9.78RR153 pKa = 11.84VRR155 pKa = 11.84EE156 pKa = 4.11GASARR161 pKa = 11.84TIAVEE166 pKa = 3.94HH167 pKa = 5.86TEE169 pKa = 4.23EE170 pKa = 4.53FIKK173 pKa = 10.79YY174 pKa = 8.83HH175 pKa = 6.22AGIEE179 pKa = 4.2RR180 pKa = 11.84AVYY183 pKa = 8.31MARR186 pKa = 11.84PEE188 pKa = 4.25PPAQRR193 pKa = 11.84QIEE196 pKa = 4.13MMIIWGPSGIGKK208 pKa = 7.22THH210 pKa = 6.11AVMMNDD216 pKa = 3.57GLSEE220 pKa = 4.18SGGVYY225 pKa = 10.08CVPTGNHH232 pKa = 6.41PWDD235 pKa = 3.66QYY237 pKa = 11.05EE238 pKa = 4.43GEE240 pKa = 4.26ATIFMDD246 pKa = 4.08EE247 pKa = 4.29FRR249 pKa = 11.84WEE251 pKa = 3.86HH252 pKa = 5.74WEE254 pKa = 4.0CTLMNRR260 pKa = 11.84ILDD263 pKa = 3.58KK264 pKa = 10.73WRR266 pKa = 11.84LQLPCRR272 pKa = 11.84YY273 pKa = 7.86MNKK276 pKa = 8.0MAAWTRR282 pKa = 11.84IIICTNQDD290 pKa = 2.76PMTWYY295 pKa = 9.82PNEE298 pKa = 4.03EE299 pKa = 4.11LPIRR303 pKa = 11.84DD304 pKa = 3.53ALRR307 pKa = 11.84RR308 pKa = 11.84RR309 pKa = 11.84LGTNCRR315 pKa = 11.84HH316 pKa = 4.31MTEE319 pKa = 4.49RR320 pKa = 11.84GQDD323 pKa = 3.53LAASPPSPDD332 pKa = 3.13FSSYY336 pKa = 10.3IPPAMRR342 pKa = 11.84VQDD345 pKa = 4.12SLDD348 pKa = 3.88DD349 pKa = 3.82ATVPASDD356 pKa = 3.45SS357 pKa = 3.42

Molecular weight: 41.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IB17|A0A0C5IB17_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 11 OX=1618234 PE=3 SV=1
MM1 pKa = 7.01VYY3 pKa = 10.36KK4 pKa = 10.47RR5 pKa = 11.84RR6 pKa = 11.84SPFKK10 pKa = 10.19KK11 pKa = 10.0LSSRR15 pKa = 11.84PRR17 pKa = 11.84KK18 pKa = 8.36RR19 pKa = 11.84ARR21 pKa = 11.84YY22 pKa = 4.08TTKK25 pKa = 8.36TARR28 pKa = 11.84RR29 pKa = 11.84ARR31 pKa = 11.84RR32 pKa = 11.84TIRR35 pKa = 11.84RR36 pKa = 11.84YY37 pKa = 9.32RR38 pKa = 11.84RR39 pKa = 11.84GYY41 pKa = 8.81KK42 pKa = 9.83ARR44 pKa = 11.84AARR47 pKa = 11.84GRR49 pKa = 11.84TNNTVANMFGLRR61 pKa = 11.84ATTKK65 pKa = 10.01SVRR68 pKa = 11.84LVHH71 pKa = 6.97SITEE75 pKa = 4.03AFAPSLAAASSVAVTAFTRR94 pKa = 11.84TINNPLDD101 pKa = 3.67PMYY104 pKa = 10.3TAGSQYY110 pKa = 10.28ATGFKK115 pKa = 10.06EE116 pKa = 3.87LAANYY121 pKa = 8.55MYY123 pKa = 11.41YY124 pKa = 10.16EE125 pKa = 4.16VQKK128 pKa = 10.84ACLDD132 pKa = 2.97IWVRR136 pKa = 11.84QSANYY141 pKa = 7.59VTAASYY147 pKa = 10.66PDD149 pKa = 4.63LIIMAKK155 pKa = 10.36LNNDD159 pKa = 3.56WTWGDD164 pKa = 3.51IQDD167 pKa = 4.84LRR169 pKa = 11.84VDD171 pKa = 3.29QWAQLANIKK180 pKa = 8.88VARR183 pKa = 11.84LRR185 pKa = 11.84FHH187 pKa = 7.6PEE189 pKa = 3.08MTGAKK194 pKa = 9.53CHH196 pKa = 6.08IRR198 pKa = 11.84LWWHH202 pKa = 5.26SKK204 pKa = 10.77KK205 pKa = 9.06NTKK208 pKa = 9.39TDD210 pKa = 3.71DD211 pKa = 3.42NVAATTASPSTIQPVAVGAFSADD234 pKa = 3.21NSTVASLPAMTISSRR249 pKa = 11.84IRR251 pKa = 11.84FWTKK255 pKa = 9.93FSMLKK260 pKa = 10.47PLDD263 pKa = 3.78TDD265 pKa = 3.79TLQDD269 pKa = 2.93IMTT272 pKa = 4.63

Molecular weight: 30.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

629

272

357

314.5

35.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.175 ± 1.86

1.59 ± 0.519

4.61 ± 0.103

5.246 ± 2.295

3.498 ± 0.115

5.405 ± 1.498

2.226 ± 0.459

6.041 ± 0.767

4.928 ± 0.803

6.2 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.293 ± 0.598

4.134 ± 0.393

5.246 ± 0.731

3.498 ± 0.338

8.744 ± 0.719

5.723 ± 0.99

7.79 ± 1.522

4.134 ± 0.839

2.544 ± 0.018

3.975 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski