Nocardioides sp. PD653

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5032 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V1VZS1|A0A1V1VZS1_9ACTN Uncharacterized protein OS=Nocardioides sp. PD653 OX=393303 GN=PD653_1060 PE=4 SV=1
MM1 pKa = 7.32LTRR4 pKa = 11.84SRR6 pKa = 11.84AAFAGLAVVAAFTLAGCGGDD26 pKa = 3.55SDD28 pKa = 5.74SDD30 pKa = 4.04VATDD34 pKa = 3.93TPSASPTGPACDD46 pKa = 4.18YY47 pKa = 10.88PADD50 pKa = 3.81AQGAAKK56 pKa = 9.96KK57 pKa = 10.26VNPPPARR64 pKa = 11.84AAVSGEE70 pKa = 3.79VGATMKK76 pKa = 10.46TSAGDD81 pKa = 3.0IGLTLDD87 pKa = 5.25ADD89 pKa = 4.15TTPCTVNSFVSLAEE103 pKa = 3.56QGYY106 pKa = 10.27FDD108 pKa = 3.83GTTCHH113 pKa = 7.23RR114 pKa = 11.84LTTTDD119 pKa = 2.73AGIFVLQCGDD129 pKa = 3.46PTGTGMGGPGYY140 pKa = 10.79SFDD143 pKa = 5.15DD144 pKa = 3.89EE145 pKa = 5.04LSGSEE150 pKa = 4.29TYY152 pKa = 10.07PAGTLAMANAGPDD165 pKa = 3.6TNGSQFFLVYY175 pKa = 10.82ADD177 pKa = 3.92TPLPASYY184 pKa = 9.55TVFGTVDD191 pKa = 3.4DD192 pKa = 5.17AGLEE196 pKa = 4.18VLKK199 pKa = 11.16GIADD203 pKa = 3.83EE204 pKa = 4.62GTADD208 pKa = 4.48GGPDD212 pKa = 3.84GPPRR216 pKa = 11.84TPVDD220 pKa = 3.41IEE222 pKa = 4.6SVTVDD227 pKa = 2.93

Molecular weight: 22.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V1WB83|A0A1V1WB83_9ACTN 3-oxoacyl-ACP reductase OS=Nocardioides sp. PD653 OX=393303 GN=PD653_4866 PE=4 SV=1
RR1 pKa = 7.53EE2 pKa = 4.15GHH4 pKa = 6.16PVAPVRR10 pKa = 11.84GGRR13 pKa = 11.84VRR15 pKa = 11.84GHH17 pKa = 5.84PRR19 pKa = 11.84RR20 pKa = 11.84PRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84GTLLRR29 pKa = 11.84PAGAGGRR36 pKa = 11.84GSPGAVRR43 pKa = 11.84PHH45 pKa = 7.28RR46 pKa = 11.84SRR48 pKa = 11.84SPPAARR54 pKa = 11.84RR55 pKa = 11.84GRR57 pKa = 11.84PVLRR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84HH64 pKa = 5.78HH65 pKa = 6.5PGLVQRR71 pKa = 11.84HH72 pKa = 4.88RR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84VRR77 pKa = 11.84HH78 pKa = 5.55PRR80 pKa = 11.84PGRR83 pKa = 11.84RR84 pKa = 11.84PRR86 pKa = 11.84PRR88 pKa = 11.84RR89 pKa = 11.84RR90 pKa = 11.84HH91 pKa = 4.53HH92 pKa = 6.58RR93 pKa = 11.84RR94 pKa = 11.84RR95 pKa = 11.84PTAGRR100 pKa = 11.84PGVRR104 pKa = 11.84RR105 pKa = 11.84LVGRR109 pKa = 11.84PPVHH113 pKa = 5.9GRR115 pKa = 11.84RR116 pKa = 11.84RR117 pKa = 11.84PRR119 pKa = 11.84QEE121 pKa = 3.26PRR123 pKa = 11.84VFGVPRR129 pKa = 11.84RR130 pKa = 11.84PAPPGRR136 pKa = 11.84AQRR139 pKa = 11.84PFGRR143 pKa = 11.84RR144 pKa = 11.84TLRR147 pKa = 11.84HH148 pKa = 5.73AGEE151 pKa = 4.6HH152 pKa = 5.36EE153 pKa = 4.76VVPLPRR159 pKa = 11.84PGPRR163 pKa = 11.84LVPRR167 pKa = 11.84LVRR170 pKa = 11.84ARR172 pKa = 11.84ASGHH176 pKa = 5.68RR177 pKa = 11.84PQRR180 pKa = 11.84TPLEE184 pKa = 4.54PGPRR188 pKa = 11.84PHH190 pKa = 6.65PTAARR195 pKa = 11.84TGEE198 pKa = 4.03PHH200 pKa = 6.85PPHH203 pKa = 6.59LRR205 pKa = 11.84VPALHH210 pKa = 6.49PTIQVLRR217 pKa = 11.84RR218 pKa = 11.84RR219 pKa = 11.84PHH221 pKa = 5.32HH222 pKa = 6.36RR223 pKa = 11.84LRR225 pKa = 11.84PEE227 pKa = 3.81RR228 pKa = 11.84THH230 pKa = 7.09LLLQPRR236 pKa = 11.84PPVPPPPPAQNPRR249 pKa = 11.84QLVVRR254 pKa = 11.84TGRR257 pKa = 11.84TPGIPLAKK265 pKa = 9.55PAGALLPPRR274 pKa = 11.84RR275 pKa = 11.84RR276 pKa = 11.84PPRR279 pKa = 11.84YY280 pKa = 8.98VV281 pKa = 2.85

Molecular weight: 32.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5032

0

5032

1565263

27

2022

311.1

33.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.818 ± 0.043

0.754 ± 0.01

6.664 ± 0.029

5.634 ± 0.031

2.797 ± 0.019

9.12 ± 0.033

2.289 ± 0.018

3.627 ± 0.025

2.019 ± 0.024

10.099 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.897 ± 0.013

1.814 ± 0.018

5.675 ± 0.029

2.823 ± 0.017

7.492 ± 0.04

5.393 ± 0.024

6.228 ± 0.029

9.313 ± 0.032

1.531 ± 0.015

2.014 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski