Diabrotica virgifera virgifera (western corn rootworm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga; Cucujiformia; Chrysomeloidea; Chrysomelidae; Galerucinae;

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25502 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P7FUQ4|A0A6P7FUQ4_DIAVI uncharacterized protein LOC114331184 OS=Diabrotica virgifera virgifera OX=50390 GN=LOC114331184 PE=4 SV=1
MM1 pKa = 7.55EE2 pKa = 4.93GFIIFLIVATVIGVSALFIKK22 pKa = 9.66TCIKK26 pKa = 10.2CFSVKK31 pKa = 10.15KK32 pKa = 10.19PRR34 pKa = 11.84QSQNNNFVSDD44 pKa = 3.54NYY46 pKa = 10.9DD47 pKa = 3.34YY48 pKa = 11.45EE49 pKa = 6.14DD50 pKa = 3.78GVIFTSEE57 pKa = 4.67LYY59 pKa = 9.3GTDD62 pKa = 3.66YY63 pKa = 11.47NVDD66 pKa = 3.42SVNEE70 pKa = 4.06VTHH73 pKa = 5.82IHH75 pKa = 6.34HH76 pKa = 7.14FEE78 pKa = 3.93TSDD81 pKa = 4.97NIDD84 pKa = 3.64TTPGDD89 pKa = 3.61TDD91 pKa = 3.42NTGSGVASGLAGGFAEE107 pKa = 4.63TFTSSFADD115 pKa = 3.59PGEE118 pKa = 4.13SSTYY122 pKa = 10.58DD123 pKa = 3.14SGGGGFDD130 pKa = 3.31SGGGGDD136 pKa = 3.5TGGGGDD142 pKa = 3.6TGGGGDD148 pKa = 3.37TGGSYY153 pKa = 10.87DD154 pKa = 3.4

Molecular weight: 15.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P7GIY1|A0A6P7GIY1_DIAVI zinc finger protein 501-like OS=Diabrotica virgifera virgifera OX=50390 GN=LOC114343270 PE=4 SV=1
VV1 pKa = 7.14KK2 pKa = 10.52NDD4 pKa = 3.64LNDD7 pKa = 3.92VILSNSEE14 pKa = 3.74DD15 pKa = 3.47GKK17 pKa = 11.02ILFQSLIDD25 pKa = 4.13PKK27 pKa = 10.21LTEE30 pKa = 4.99PPPPPPPTDD39 pKa = 3.33PLGPPEE45 pKa = 4.82PPSDD49 pKa = 3.89VPEE52 pKa = 4.57DD53 pKa = 3.83LSNKK57 pKa = 9.5RR58 pKa = 11.84PRR60 pKa = 11.84PPPKK64 pKa = 9.87ALSSKK69 pKa = 10.24RR70 pKa = 11.84PRR72 pKa = 11.84PPPSKK77 pKa = 10.32RR78 pKa = 11.84PRR80 pKa = 11.84TTSPPPSRR88 pKa = 11.84RR89 pKa = 11.84PKK91 pKa = 10.5PPGPPPTRR99 pKa = 11.84RR100 pKa = 11.84PRR102 pKa = 11.84PPGPPPSRR110 pKa = 11.84RR111 pKa = 11.84PRR113 pKa = 11.84PPGPPPSRR121 pKa = 11.84RR122 pKa = 11.84PRR124 pKa = 11.84PPGPPPSRR132 pKa = 11.84RR133 pKa = 11.84PRR135 pKa = 11.84PPGPPPSRR143 pKa = 11.84RR144 pKa = 11.84PRR146 pKa = 11.84PPGPPPSRR154 pKa = 11.84RR155 pKa = 11.84PRR157 pKa = 11.84PPGPPPSRR165 pKa = 11.84RR166 pKa = 11.84PRR168 pKa = 11.84PPGPPPSRR176 pKa = 11.84RR177 pKa = 11.84PRR179 pKa = 11.84PPGPPPSRR187 pKa = 11.84RR188 pKa = 11.84PRR190 pKa = 11.84PPGPPPSRR198 pKa = 11.84RR199 pKa = 11.84PRR201 pKa = 11.84PPGPPPSRR209 pKa = 11.84RR210 pKa = 11.84PRR212 pKa = 11.84PPGPPPSRR220 pKa = 11.84RR221 pKa = 11.84PRR223 pKa = 11.84PPGPPPSRR231 pKa = 11.84RR232 pKa = 11.84PRR234 pKa = 11.84PPGPPPSRR242 pKa = 11.84RR243 pKa = 11.84PRR245 pKa = 11.84PPGPPPSRR253 pKa = 11.84RR254 pKa = 11.84PRR256 pKa = 11.84PPGPPPSRR264 pKa = 11.84RR265 pKa = 11.84PRR267 pKa = 11.84PPGPPPSRR275 pKa = 11.84RR276 pKa = 11.84PRR278 pKa = 11.84PPGPPPSRR286 pKa = 11.84RR287 pKa = 11.84PRR289 pKa = 11.84PPGPPPSRR297 pKa = 11.84RR298 pKa = 11.84PRR300 pKa = 11.84PPGPPPSRR308 pKa = 11.84RR309 pKa = 11.84PP310 pKa = 3.28

Molecular weight: 33.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20165

5337

25502

12442332

30

19839

487.9

55.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.294 ± 0.017

2.167 ± 0.025

5.498 ± 0.016

7.113 ± 0.023

3.978 ± 0.014

5.116 ± 0.023

2.513 ± 0.011

6.17 ± 0.013

7.647 ± 0.025

8.747 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.14 ± 0.01

5.555 ± 0.019

4.875 ± 0.019

4.251 ± 0.019

4.72 ± 0.017

7.905 ± 0.021

6.073 ± 0.027

5.998 ± 0.015

0.958 ± 0.006

3.276 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski