Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Citrobacter; Citrobacter koseri

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5019 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8AKA3|A8AKA3_CITK8 ABC transmembrane type-1 domain-containing protein OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) OX=290338 GN=CKO_02810 PE=3 SV=1
MM1 pKa = 7.37HH2 pKa = 6.88SWKK5 pKa = 10.29KK6 pKa = 10.49KK7 pKa = 9.88LVVSQLALACTLAITSQANASTDD30 pKa = 3.14ISGTTYY36 pKa = 8.15TTFNHH41 pKa = 6.08YY42 pKa = 10.94NDD44 pKa = 3.27ATYY47 pKa = 11.45ADD49 pKa = 3.54GVYY52 pKa = 10.55YY53 pKa = 10.6DD54 pKa = 5.91GYY56 pKa = 11.26VGWNNYY62 pKa = 7.95ATDD65 pKa = 3.4SVYY68 pKa = 11.18NGDD71 pKa = 3.99IYY73 pKa = 10.88PVINNATVNGVISTYY88 pKa = 11.01YY89 pKa = 10.75LDD91 pKa = 5.97DD92 pKa = 4.34GLSTNTNSNSLTIKK106 pKa = 10.46NSTIHH111 pKa = 6.74GMITSEE117 pKa = 5.7CMTTDD122 pKa = 3.19CVGRR126 pKa = 11.84DD127 pKa = 3.38ATGYY131 pKa = 10.62VYY133 pKa = 10.81DD134 pKa = 4.51RR135 pKa = 11.84LALTVDD141 pKa = 3.46NSTIDD146 pKa = 3.71DD147 pKa = 3.99NYY149 pKa = 11.03EE150 pKa = 3.75HH151 pKa = 6.27YY152 pKa = 9.43TYY154 pKa = 11.16NGTYY158 pKa = 9.91TDD160 pKa = 3.91GTADD164 pKa = 3.28THH166 pKa = 7.24VVDD169 pKa = 5.12VYY171 pKa = 11.89NLGTAITLDD180 pKa = 3.51QEE182 pKa = 3.92VDD184 pKa = 3.74LVIKK188 pKa = 10.62NNSHH192 pKa = 5.43VAGITLTQGYY202 pKa = 8.14EE203 pKa = 3.79WEE205 pKa = 5.73DD206 pKa = 3.2IDD208 pKa = 6.2DD209 pKa = 4.15NTVSTGVNSAEE220 pKa = 4.11VFNNTVTVTDD230 pKa = 3.63STVTSGSWSDD240 pKa = 3.49EE241 pKa = 3.9GTSGWFGNTNNASDD255 pKa = 4.14YY256 pKa = 10.24SDD258 pKa = 5.06SYY260 pKa = 10.42TADD263 pKa = 3.4DD264 pKa = 3.7VAIAAIAHH272 pKa = 6.19PNADD276 pKa = 3.04NAMQTTVNLSNSTLMGDD293 pKa = 3.78VVFSSNFDD301 pKa = 3.48EE302 pKa = 5.33NFFPNGADD310 pKa = 3.41TYY312 pKa = 11.03RR313 pKa = 11.84DD314 pKa = 3.14TDD316 pKa = 3.75ADD318 pKa = 4.56LDD320 pKa = 4.29TNGWDD325 pKa = 3.47GTDD328 pKa = 3.71RR329 pKa = 11.84MDD331 pKa = 3.14VTLNNGSKK339 pKa = 9.47WVGAAMSVHH348 pKa = 5.8QVDD351 pKa = 3.33SDD353 pKa = 3.81GDD355 pKa = 3.83GVYY358 pKa = 10.9DD359 pKa = 4.0SFAAGTDD366 pKa = 3.41ATATLIDD373 pKa = 3.52IAANSLWPSSTYY385 pKa = 11.0GIDD388 pKa = 3.95NNDD391 pKa = 3.02TAYY394 pKa = 10.63GEE396 pKa = 4.72DD397 pKa = 3.26GHH399 pKa = 7.61VVGNEE404 pKa = 4.1VYY406 pKa = 10.48QSGLFNVTLNGGSQWDD422 pKa = 3.84TTKK425 pKa = 10.85TSLIDD430 pKa = 3.4TLSINSGSVVNVADD444 pKa = 3.84SDD446 pKa = 4.34LVSDD450 pKa = 5.06SISLTGGAALNINEE464 pKa = 5.08DD465 pKa = 3.42GHH467 pKa = 6.03VATDD471 pKa = 3.47EE472 pKa = 4.06LTINNSTVTIADD484 pKa = 3.86DD485 pKa = 4.15VSAGWGVYY493 pKa = 9.5DD494 pKa = 4.01AALYY498 pKa = 11.08ANTINVTNNGVLDD511 pKa = 3.97VGNSTAYY518 pKa = 10.41ALQADD523 pKa = 4.43TLNLTSYY530 pKa = 10.6TDD532 pKa = 3.38ANGNVNAGVFNVHH545 pKa = 6.3SNSFVLDD552 pKa = 3.52ADD554 pKa = 3.95LTNDD558 pKa = 3.33RR559 pKa = 11.84TNDD562 pKa = 3.17ITKK565 pKa = 10.28SNYY568 pKa = 9.27GYY570 pKa = 11.19GVIAMNSDD578 pKa = 2.85GHH580 pKa = 5.83LTINGNGDD588 pKa = 3.08AWTGDD593 pKa = 3.25QSEE596 pKa = 4.42VDD598 pKa = 3.37NAGDD602 pKa = 3.43NVAAATGNYY611 pKa = 8.97KK612 pKa = 10.02VRR614 pKa = 11.84IDD616 pKa = 3.45NATGEE621 pKa = 4.47GSVADD626 pKa = 3.94YY627 pKa = 10.58KK628 pKa = 11.15GKK630 pKa = 10.29EE631 pKa = 3.97LIYY634 pKa = 11.34VNDD637 pKa = 4.19KK638 pKa = 10.67NSKK641 pKa = 8.67ATFSAANKK649 pKa = 9.86ADD651 pKa = 3.72LGAYY655 pKa = 7.63TYY657 pKa = 10.27QAQQEE662 pKa = 4.68GNTVVMQQMEE672 pKa = 4.41LTDD675 pKa = 4.11YY676 pKa = 11.84ANMALSIPSANTNIWNLEE694 pKa = 3.6QDD696 pKa = 3.86TVGTRR701 pKa = 11.84LTNSRR706 pKa = 11.84HH707 pKa = 4.84GLADD711 pKa = 3.5NGGAWVSYY719 pKa = 10.31FGGSFDD725 pKa = 5.18GDD727 pKa = 3.32NGTINYY733 pKa = 8.82DD734 pKa = 3.13QDD736 pKa = 3.61VNGIMVGVDD745 pKa = 2.97TKK747 pKa = 11.02IDD749 pKa = 3.49GNNAKK754 pKa = 9.7WIVGAAAGFAKK765 pKa = 10.6GDD767 pKa = 3.43MSDD770 pKa = 2.99RR771 pKa = 11.84TGQVDD776 pKa = 3.54QDD778 pKa = 3.84SQSAYY783 pKa = 8.65IYY785 pKa = 10.6SSARR789 pKa = 11.84FANNVFVDD797 pKa = 4.71GSLSYY802 pKa = 11.41SHH804 pKa = 7.31FNNDD808 pKa = 3.29LSANMSNGQYY818 pKa = 10.78VDD820 pKa = 3.92GNTSADD826 pKa = 2.84AWGFGLKK833 pKa = 10.32LGYY836 pKa = 9.47DD837 pKa = 3.67WKK839 pKa = 11.09LGDD842 pKa = 3.96AGYY845 pKa = 8.07VTPYY849 pKa = 10.65GSVSGLFQSGDD860 pKa = 3.79DD861 pKa = 3.89YY862 pKa = 11.83QLSNDD867 pKa = 3.96MKK869 pKa = 11.23VDD871 pKa = 3.62GQSYY875 pKa = 10.64DD876 pKa = 3.02SMRR879 pKa = 11.84YY880 pKa = 8.94EE881 pKa = 4.34LGVDD885 pKa = 3.21AGYY888 pKa = 8.53TFTYY892 pKa = 10.6SEE894 pKa = 4.47DD895 pKa = 3.66QALTPYY901 pKa = 10.37FKK903 pKa = 10.64LAYY906 pKa = 10.48VYY908 pKa = 11.28DD909 pKa = 4.46DD910 pKa = 4.6ANNDD914 pKa = 3.18ADD916 pKa = 4.23VNGDD920 pKa = 3.84SIDD923 pKa = 3.7NGVEE927 pKa = 3.97GSAVRR932 pKa = 11.84VGLGTQFSFTKK943 pKa = 10.49NFSAYY948 pKa = 9.3TDD950 pKa = 3.45ANYY953 pKa = 10.81LGGGDD958 pKa = 4.6VDD960 pKa = 4.32QDD962 pKa = 2.67WSANVGVKK970 pKa = 7.43YY971 pKa = 8.84TWW973 pKa = 3.09

Molecular weight: 103.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8AMV3|A8AMV3_CITK8 Uncharacterized protein OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) OX=290338 GN=CKO_03740 PE=4 SV=1
MM1 pKa = 6.45VARR4 pKa = 11.84WRR6 pKa = 11.84KK7 pKa = 9.95LIGPTISPHH16 pKa = 6.17RR17 pKa = 11.84STVGRR22 pKa = 11.84IRR24 pKa = 11.84RR25 pKa = 11.84SRR27 pKa = 11.84HH28 pKa = 4.65PALPAPHH35 pKa = 7.43RR36 pKa = 11.84STVGRR41 pKa = 11.84IRR43 pKa = 11.84RR44 pKa = 11.84SHH46 pKa = 6.56HH47 pKa = 6.09PALPAPHH54 pKa = 7.3RR55 pKa = 11.84NTVGRR60 pKa = 11.84IRR62 pKa = 11.84RR63 pKa = 11.84SRR65 pKa = 11.84HH66 pKa = 4.9PALLPGIPARR76 pKa = 11.84FF77 pKa = 3.37

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5019

0

5019

1442289

27

3208

287.4

31.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.746 ± 0.048

1.176 ± 0.015

5.149 ± 0.026

5.506 ± 0.033

3.963 ± 0.027

7.386 ± 0.035

2.334 ± 0.017

5.862 ± 0.028

4.132 ± 0.031

10.754 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.811 ± 0.018

3.791 ± 0.027

4.447 ± 0.031

4.477 ± 0.031

5.788 ± 0.033

5.876 ± 0.026

5.446 ± 0.025

7.08 ± 0.03

1.503 ± 0.015

2.775 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski