Rhizobium phage RHEph06

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Kleczkowskavirus; unclassified Kleczkowskavirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7TLH7|L7TLH7_9CAUD Uncharacterized protein OS=Rhizobium phage RHEph06 OX=1220714 GN=RHEph06_gp043 PE=4 SV=1
MM1 pKa = 7.97PYY3 pKa = 10.0VQRR6 pKa = 11.84DD7 pKa = 3.64SEE9 pKa = 5.0GNISWMYY16 pKa = 10.79LNFGEE21 pKa = 5.62GYY23 pKa = 10.71AEE25 pKa = 4.45EE26 pKa = 4.71FLPDD30 pKa = 4.16DD31 pKa = 4.18SPEE34 pKa = 3.8VLAFVLSRR42 pKa = 11.84RR43 pKa = 11.84PSPYY47 pKa = 9.2TVSLALMWSRR57 pKa = 11.84MTDD60 pKa = 3.17TEE62 pKa = 4.56AEE64 pKa = 4.25LVQQAIDD71 pKa = 4.05LLPVRR76 pKa = 11.84AQNIIAATDD85 pKa = 3.58APFSDD90 pKa = 3.71SDD92 pKa = 3.71AFGWLRR98 pKa = 11.84TAIEE102 pKa = 4.62DD103 pKa = 3.47ATSASRR109 pKa = 11.84ADD111 pKa = 4.81EE112 pKa = 3.84IMAWFMVILL121 pKa = 4.24

Molecular weight: 13.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7TMV1|L7TMV1_9CAUD Putative terminase protein large subunit OS=Rhizobium phage RHEph06 OX=1220714 GN=RHEph06_gp010 PE=4 SV=1
MM1 pKa = 7.36SEE3 pKa = 4.03LMRR6 pKa = 11.84RR7 pKa = 11.84LARR10 pKa = 11.84SAKK13 pKa = 9.9RR14 pKa = 11.84GEE16 pKa = 4.55GCHH19 pKa = 6.06LTPEE23 pKa = 4.41MVRR26 pKa = 11.84EE27 pKa = 4.1LVSMRR32 pKa = 11.84TIRR35 pKa = 11.84RR36 pKa = 11.84HH37 pKa = 4.31IAEE40 pKa = 4.35EE41 pKa = 3.8RR42 pKa = 11.84RR43 pKa = 11.84PPKK46 pKa = 9.79PRR48 pKa = 11.84PEE50 pKa = 4.05PAVVTNKK57 pKa = 9.54MIRR60 pKa = 11.84EE61 pKa = 3.65IRR63 pKa = 11.84EE64 pKa = 3.97LKK66 pKa = 9.89RR67 pKa = 11.84ANPRR71 pKa = 11.84MLRR74 pKa = 11.84RR75 pKa = 11.84EE76 pKa = 3.4IAEE79 pKa = 3.86IVGCSVATVQKK90 pKa = 10.69YY91 pKa = 9.97SSEE94 pKa = 4.16KK95 pKa = 9.55NLEE98 pKa = 3.69IAKK101 pKa = 9.64QRR103 pKa = 11.84EE104 pKa = 4.11AEE106 pKa = 4.07RR107 pKa = 11.84YY108 pKa = 9.14ALNKK112 pKa = 10.35DD113 pKa = 3.46RR114 pKa = 11.84MVSEE118 pKa = 3.85ALARR122 pKa = 11.84YY123 pKa = 8.34HH124 pKa = 6.52RR125 pKa = 11.84KK126 pKa = 9.07KK127 pKa = 10.48AARR130 pKa = 11.84EE131 pKa = 4.2TQCSII136 pKa = 3.92

Molecular weight: 15.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

16604

38

813

202.5

22.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.672 ± 0.601

1.12 ± 0.134

6.005 ± 0.205

5.968 ± 0.314

3.716 ± 0.196

7.655 ± 0.301

1.849 ± 0.146

5.764 ± 0.204

5.408 ± 0.284

7.998 ± 0.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.584 ± 0.137

3.975 ± 0.174

4.571 ± 0.261

3.746 ± 0.218

5.776 ± 0.255

5.722 ± 0.236

6.191 ± 0.257

6.715 ± 0.21

1.62 ± 0.116

2.945 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski