Borrelia duttonii CR2A

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Borreliaceae; Borrelia; Borrelia duttonii

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1995 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6TJQ3|W6TJQ3_9SPIR Ribonuclease Z OS=Borrelia duttonii CR2A OX=1432657 GN=BDCR2A_00926 PE=4 SV=1
MM1 pKa = 6.97KK2 pKa = 9.72TINFTLILLLLMSNCEE18 pKa = 3.64QDD20 pKa = 4.5NNINDD25 pKa = 4.77DD26 pKa = 3.33IEE28 pKa = 4.64YY29 pKa = 10.28IQLQLL34 pKa = 3.81

Molecular weight: 4.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6TJH6|W6TJH6_9SPIR Uncharacterized protein OS=Borrelia duttonii CR2A OX=1432657 GN=BDCR2A_00816 PE=4 SV=1
MM1 pKa = 7.67AVPKK5 pKa = 9.82FKK7 pKa = 10.16PSKK10 pKa = 9.35SRR12 pKa = 11.84SRR14 pKa = 11.84TRR16 pKa = 11.84RR17 pKa = 11.84SINMRR22 pKa = 11.84KK23 pKa = 9.83KK24 pKa = 9.1IPQFQEE30 pKa = 3.95CSNCGNLAIRR40 pKa = 11.84HH41 pKa = 6.27RR42 pKa = 11.84ICAKK46 pKa = 9.54CGYY49 pKa = 8.75YY50 pKa = 10.33RR51 pKa = 11.84NSQYY55 pKa = 11.49LEE57 pKa = 4.29LGLL60 pKa = 4.64

Molecular weight: 7.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1995

0

1995

448606

20

2438

224.9

25.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.026 ± 0.081

0.803 ± 0.019

5.695 ± 0.061

6.757 ± 0.066

5.431 ± 0.064

5.36 ± 0.088

1.348 ± 0.022

10.073 ± 0.087

10.081 ± 0.083

9.911 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.234 ± 0.03

7.263 ± 0.078

2.189 ± 0.033

2.687 ± 0.044

3.452 ± 0.043

6.913 ± 0.051

4.334 ± 0.05

5.833 ± 0.078

0.475 ± 0.015

4.134 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski