Amino acid dipepetide frequency for Desulfobulbus sp. Tol-SR

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.703AlaAla: 11.703 ± 0.167	1.325AlaCys: 1.325 ± 0.038	5.518AlaAsp: 5.518 ± 0.082	6.328AlaGlu: 6.328 ± 0.09	3.484AlaPhe: 3.484 ± 0.059	8.764AlaGly: 8.764 ± 0.113	1.689AlaHis: 1.689 ± 0.04	6.151AlaIle: 6.151 ± 0.088	3.6AlaLys: 3.6 ± 0.073	9.347AlaLeu: 9.347 ± 0.119	2.933AlaMet: 2.933 ± 0.05	2.402AlaAsn: 2.402 ± 0.051	3.269AlaPro: 3.269 ± 0.073	2.498AlaGln: 2.498 ± 0.049	6.173AlaArg: 6.173 ± 0.088	4.49AlaSer: 4.49 ± 0.069	4.942AlaThr: 4.942 ± 0.065	7.624AlaVal: 7.624 ± 0.114	1.014AlaTrp: 1.014 ± 0.033	2.179AlaTyr: 2.179 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	1.132CysAla: 1.132 ± 0.033	0.255CysCys: 0.255 ± 0.018	0.682CysAsp: 0.682 ± 0.028	0.598CysGlu: 0.598 ± 0.026	0.503CysPhe: 0.503 ± 0.022	1.424CysGly: 1.424 ± 0.042	0.458CysHis: 0.458 ± 0.027	0.723CysIle: 0.723 ± 0.03	0.34CysLys: 0.34 ± 0.018	1.306CysLeu: 1.306 ± 0.034	0.316CysMet: 0.316 ± 0.016	0.437CysAsn: 0.437 ± 0.021	0.905CysPro: 0.905 ± 0.034	0.398CysGln: 0.398 ± 0.017	1.262CysArg: 1.262 ± 0.036	0.85CysSer: 0.85 ± 0.028	0.648CysThr: 0.648 ± 0.025	0.762CysVal: 0.762 ± 0.03	0.152CysTrp: 0.152 ± 0.011	0.362CysTyr: 0.362 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	4.328AspAla: 4.328 ± 0.073	0.771AspCys: 0.771 ± 0.03	3.082AspAsp: 3.082 ± 0.068	3.489AspGlu: 3.489 ± 0.065	2.421AspPhe: 2.421 ± 0.052	4.362AspGly: 4.362 ± 0.076	1.31AspHis: 1.31 ± 0.035	3.904AspIle: 3.904 ± 0.065	2.328AspLys: 2.328 ± 0.049	6.327AspLeu: 6.327 ± 0.085	1.348AspMet: 1.348 ± 0.037	1.767AspAsn: 1.767 ± 0.042	2.876AspPro: 2.876 ± 0.056	1.947AspGln: 1.947 ± 0.051	4.182AspArg: 4.182 ± 0.069	2.779AspSer: 2.779 ± 0.053	2.531AspThr: 2.531 ± 0.05	3.328AspVal: 3.328 ± 0.061	0.703AspTrp: 0.703 ± 0.029	1.697AspTyr: 1.697 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	5.898GluAla: 5.898 ± 0.093	0.561GluCys: 0.561 ± 0.023	2.892GluAsp: 2.892 ± 0.056	4.526GluGlu: 4.526 ± 0.095	2.123GluPhe: 2.123 ± 0.045	4.122GluGly: 4.122 ± 0.077	1.323GluHis: 1.323 ± 0.04	4.843GluIle: 4.843 ± 0.076	3.878GluLys: 3.878 ± 0.071	6.115GluLeu: 6.115 ± 0.091	1.97GluMet: 1.97 ± 0.047	2.135GluAsn: 2.135 ± 0.05	2.275GluPro: 2.275 ± 0.058	2.615GluGln: 2.615 ± 0.064	4.393GluArg: 4.393 ± 0.075	2.875GluSer: 2.875 ± 0.049	3.247GluThr: 3.247 ± 0.059	4.449GluVal: 4.449 ± 0.067	0.672GluTrp: 0.672 ± 0.026	1.725GluTyr: 1.725 ± 0.045	0.001GluXaa: 0.001 ± 0.001
Phe	3.791PheAla: 3.791 ± 0.072	0.641PheCys: 0.641 ± 0.024	2.468PheAsp: 2.468 ± 0.054	2.173PheGlu: 2.173 ± 0.048	1.99PhePhe: 1.99 ± 0.049	3.286PheGly: 3.286 ± 0.069	0.895PheHis: 0.895 ± 0.032	2.329PheIle: 2.329 ± 0.058	1.496PheLys: 1.496 ± 0.044	4.227PheLeu: 4.227 ± 0.07	0.858PheMet: 0.858 ± 0.025	1.392PheAsn: 1.392 ± 0.039	1.794PhePro: 1.794 ± 0.04	1.202PheGln: 1.202 ± 0.035	2.39PheArg: 2.39 ± 0.058	2.765PheSer: 2.765 ± 0.067	2.284PheThr: 2.284 ± 0.054	2.739PheVal: 2.739 ± 0.059	0.501PheTrp: 0.501 ± 0.021	1.098PheTyr: 1.098 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	6.44GlyAla: 6.44 ± 0.112	1.289GlyCys: 1.289 ± 0.04	4.096GlyAsp: 4.096 ± 0.066	4.711GlyGlu: 4.711 ± 0.072	3.318GlyPhe: 3.318 ± 0.065	6.413GlyGly: 6.413 ± 0.094	1.713GlyHis: 1.713 ± 0.042	5.53GlyIle: 5.53 ± 0.085	4.144GlyLys: 4.144 ± 0.082	8.015GlyLeu: 8.015 ± 0.101	2.517GlyMet: 2.517 ± 0.052	2.46GlyAsn: 2.46 ± 0.056	2.689GlyPro: 2.689 ± 0.052	2.679GlyGln: 2.679 ± 0.052	6.092GlyArg: 6.092 ± 0.099	4.305GlySer: 4.305 ± 0.067	4.241GlyThr: 4.241 ± 0.066	5.405GlyVal: 5.405 ± 0.091	1.03GlyTrp: 1.03 ± 0.037	2.56GlyTyr: 2.56 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.688HisAla: 1.688 ± 0.044	0.388HisCys: 0.388 ± 0.02	1.216HisAsp: 1.216 ± 0.033	1.103HisGlu: 1.103 ± 0.035	0.994HisPhe: 0.994 ± 0.033	1.935HisGly: 1.935 ± 0.045	0.658HisHis: 0.658 ± 0.032	1.321HisIle: 1.321 ± 0.039	0.704HisLys: 0.704 ± 0.028	2.409HisLeu: 2.409 ± 0.05	0.414HisMet: 0.414 ± 0.02	0.672HisAsn: 0.672 ± 0.024	1.44HisPro: 1.44 ± 0.038	0.779HisGln: 0.779 ± 0.025	1.648HisArg: 1.648 ± 0.04	1.193HisSer: 1.193 ± 0.039	0.977HisThr: 0.977 ± 0.032	1.287HisVal: 1.287 ± 0.034	0.266HisTrp: 0.266 ± 0.015	0.685HisTyr: 0.685 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.255IleAla: 6.255 ± 0.091	0.907IleCys: 0.907 ± 0.032	4.251IleAsp: 4.251 ± 0.067	4.221IleGlu: 4.221 ± 0.072	2.596IlePhe: 2.596 ± 0.057	5.289IleGly: 5.289 ± 0.091	1.516IleHis: 1.516 ± 0.045	3.833IleIle: 3.833 ± 0.075	2.465IleLys: 2.465 ± 0.06	6.242IleLeu: 6.242 ± 0.092	1.416IleMet: 1.416 ± 0.043	2.143IleAsn: 2.143 ± 0.049	3.01IlePro: 3.01 ± 0.052	1.807IleGln: 1.807 ± 0.045	4.09IleArg: 4.09 ± 0.065	3.696IleSer: 3.696 ± 0.064	3.471IleThr: 3.471 ± 0.061	4.807IleVal: 4.807 ± 0.084	0.541IleTrp: 0.541 ± 0.026	1.498IleTyr: 1.498 ± 0.041	0.0IleXaa: 0.0 ± 0.0
Lys	4.114LysAla: 4.114 ± 0.08	0.412LysCys: 0.412 ± 0.02	2.399LysAsp: 2.399 ± 0.053	3.152LysGlu: 3.152 ± 0.073	1.261LysPhe: 1.261 ± 0.033	3.242LysGly: 3.242 ± 0.06	0.755LysHis: 0.755 ± 0.027	3.154LysIle: 3.154 ± 0.067	3.033LysLys: 3.033 ± 0.074	3.616LysLeu: 3.616 ± 0.072	1.325LysMet: 1.325 ± 0.034	1.665LysAsn: 1.665 ± 0.048	1.783LysPro: 1.783 ± 0.047	1.434LysGln: 1.434 ± 0.041	2.609LysArg: 2.609 ± 0.058	2.216LysSer: 2.216 ± 0.042	2.545LysThr: 2.545 ± 0.054	3.166LysVal: 3.166 ± 0.07	0.429LysTrp: 0.429 ± 0.022	1.114LysTyr: 1.114 ± 0.036	0.0LysXaa: 0.0 ± 0.0
Leu	11.507LeuAla: 11.507 ± 0.143	1.303LeuCys: 1.303 ± 0.038	5.796LeuAsp: 5.796 ± 0.088	6.269LeuGlu: 6.269 ± 0.085	4.245LeuPhe: 4.245 ± 0.082	7.473LeuGly: 7.473 ± 0.099	2.26LeuHis: 2.26 ± 0.052	5.703LeuIle: 5.703 ± 0.081	4.525LeuLys: 4.525 ± 0.077	11.333LeuLeu: 11.333 ± 0.138	2.275LeuMet: 2.275 ± 0.042	2.927LeuAsn: 2.927 ± 0.056	5.317LeuPro: 5.317 ± 0.083	4.033LeuGln: 4.033 ± 0.074	6.373LeuArg: 6.373 ± 0.097	5.964LeuSer: 5.964 ± 0.081	5.488LeuThr: 5.488 ± 0.083	7.41LeuVal: 7.41 ± 0.098	0.974LeuTrp: 0.974 ± 0.033	2.514LeuTyr: 2.514 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	2.945MetAla: 2.945 ± 0.065	0.224MetCys: 0.224 ± 0.016	1.37MetAsp: 1.37 ± 0.043	1.686MetGlu: 1.686 ± 0.047	0.849MetPhe: 0.849 ± 0.03	1.871MetGly: 1.871 ± 0.052	0.516MetHis: 0.516 ± 0.024	1.606MetIle: 1.606 ± 0.039	1.473MetLys: 1.473 ± 0.043	2.453MetLeu: 2.453 ± 0.052	0.707MetMet: 0.707 ± 0.027	1.028MetAsn: 1.028 ± 0.032	1.236MetPro: 1.236 ± 0.04	0.949MetGln: 0.949 ± 0.031	1.498MetArg: 1.498 ± 0.041	1.432MetSer: 1.432 ± 0.042	1.723MetThr: 1.723 ± 0.042	1.985MetVal: 1.985 ± 0.044	0.178MetTrp: 0.178 ± 0.013	0.508MetTyr: 0.508 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	2.382AsnAla: 2.382 ± 0.055	0.464AsnCys: 0.464 ± 0.027	1.585AsnAsp: 1.585 ± 0.039	1.556AsnGlu: 1.556 ± 0.039	1.234AsnPhe: 1.234 ± 0.039	2.44AsnGly: 2.44 ± 0.057	0.69AsnHis: 0.69 ± 0.025	2.211AsnIle: 2.211 ± 0.048	1.209AsnLys: 1.209 ± 0.037	3.379AsnLeu: 3.379 ± 0.057	0.775AsnMet: 0.775 ± 0.031	0.964AsnAsn: 0.964 ± 0.033	1.962AsnPro: 1.962 ± 0.046	1.014AsnGln: 1.014 ± 0.033	2.426AsnArg: 2.426 ± 0.049	1.584AsnSer: 1.584 ± 0.041	1.514AsnThr: 1.514 ± 0.037	1.913AsnVal: 1.913 ± 0.047	0.394AsnTrp: 0.394 ± 0.021	0.919AsnTyr: 0.919 ± 0.031	0.001AsnXaa: 0.001 ± 0.001
Pro	4.805ProAla: 4.805 ± 0.082	0.54ProCys: 0.54 ± 0.028	3.015ProAsp: 3.015 ± 0.057	3.569ProGlu: 3.569 ± 0.065	1.974ProPhe: 1.974 ± 0.044	4.057ProGly: 4.057 ± 0.071	0.903ProHis: 0.903 ± 0.03	2.189ProIle: 2.189 ± 0.045	1.672ProLys: 1.672 ± 0.043	4.609ProLeu: 4.609 ± 0.081	1.073ProMet: 1.073 ± 0.026	1.103ProAsn: 1.103 ± 0.035	2.344ProPro: 2.344 ± 0.063	1.561ProGln: 1.561 ± 0.042	2.405ProArg: 2.405 ± 0.055	2.21ProSer: 2.21 ± 0.045	2.146ProThr: 2.146 ± 0.05	3.861ProVal: 3.861 ± 0.069	0.625ProTrp: 0.625 ± 0.026	1.203ProTyr: 1.203 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.695GlnAla: 3.695 ± 0.082	0.373GlnCys: 0.373 ± 0.02	1.681GlnAsp: 1.681 ± 0.04	2.352GlnGlu: 2.352 ± 0.06	1.248GlnPhe: 1.248 ± 0.036	2.661GlnGly: 2.661 ± 0.052	0.743GlnHis: 0.743 ± 0.023	2.086GlnIle: 2.086 ± 0.051	1.592GlnLys: 1.592 ± 0.044	3.457GlnLeu: 3.457 ± 0.072	0.887GlnMet: 0.887 ± 0.034	0.935GlnAsn: 0.935 ± 0.032	1.643GlnPro: 1.643 ± 0.038	1.646GlnGln: 1.646 ± 0.053	2.318GlnArg: 2.318 ± 0.053	1.672GlnSer: 1.672 ± 0.041	1.731GlnThr: 1.731 ± 0.045	2.553GlnVal: 2.553 ± 0.051	0.421GlnTrp: 0.421 ± 0.02	0.981GlnTyr: 0.981 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	4.739ArgAla: 4.739 ± 0.082	0.879ArgCys: 0.879 ± 0.03	3.658ArgAsp: 3.658 ± 0.062	4.552ArgGlu: 4.552 ± 0.073	2.944ArgPhe: 2.944 ± 0.054	4.086ArgGly: 4.086 ± 0.068	1.766ArgHis: 1.766 ± 0.048	4.8ArgIle: 4.8 ± 0.075	3.091ArgLys: 3.091 ± 0.061	7.765ArgLeu: 7.765 ± 0.106	1.938ArgMet: 1.938 ± 0.046	2.188ArgAsn: 2.188 ± 0.046	2.905ArgPro: 2.905 ± 0.059	3.326ArgGln: 3.326 ± 0.067	5.218ArgArg: 5.218 ± 0.107	3.438ArgSer: 3.438 ± 0.062	3.231ArgThr: 3.231 ± 0.056	4.082ArgVal: 4.082 ± 0.072	0.774ArgTrp: 0.774 ± 0.034	2.185ArgTyr: 2.185 ± 0.055	0.0ArgXaa: 0.0 ± 0.0
Ser	4.548SerAla: 4.548 ± 0.074	0.846SerCys: 0.846 ± 0.03	2.573SerAsp: 2.573 ± 0.055	2.842SerGlu: 2.842 ± 0.054	2.375SerPhe: 2.375 ± 0.047	5.165SerGly: 5.165 ± 0.079	1.162SerHis: 1.162 ± 0.035	3.323SerIle: 3.323 ± 0.059	1.874SerLys: 1.874 ± 0.046	5.963SerLeu: 5.963 ± 0.071	1.5SerMet: 1.5 ± 0.04	1.418SerAsn: 1.418 ± 0.038	2.665SerPro: 2.665 ± 0.052	1.71SerGln: 1.71 ± 0.04	3.878SerArg: 3.878 ± 0.059	3.034SerSer: 3.034 ± 0.062	2.832SerThr: 2.832 ± 0.057	3.461SerVal: 3.461 ± 0.059	0.715SerTrp: 0.715 ± 0.03	1.584SerTyr: 1.584 ± 0.043	0.001SerXaa: 0.001 ± 0.001
Thr	5.404ThrAla: 5.404 ± 0.07	0.684ThrCys: 0.684 ± 0.026	2.708ThrAsp: 2.708 ± 0.052	2.874ThrGlu: 2.874 ± 0.056	2.066ThrPhe: 2.066 ± 0.044	5.076ThrGly: 5.076 ± 0.073	0.998ThrHis: 0.998 ± 0.034	3.57ThrIle: 3.57 ± 0.057	1.608ThrLys: 1.608 ± 0.04	5.41ThrLeu: 5.41 ± 0.074	1.359ThrMet: 1.359 ± 0.042	1.384ThrAsn: 1.384 ± 0.041	2.656ThrPro: 2.656 ± 0.056	1.216ThrGln: 1.216 ± 0.037	3.096ThrArg: 3.096 ± 0.066	2.759ThrSer: 2.759 ± 0.048	2.975ThrThr: 2.975 ± 0.069	4.455ThrVal: 4.455 ± 0.077	0.549ThrTrp: 0.549 ± 0.023	1.342ThrTyr: 1.342 ± 0.044	0.001ThrXaa: 0.001 ± 0.001
Val	6.937ValAla: 6.937 ± 0.081	1.056ValCys: 1.056 ± 0.035	4.322ValAsp: 4.322 ± 0.065	4.618ValGlu: 4.618 ± 0.077	2.972ValPhe: 2.972 ± 0.061	5.092ValGly: 5.092 ± 0.082	1.493ValHis: 1.493 ± 0.039	4.624ValIle: 4.624 ± 0.073	3.001ValLys: 3.001 ± 0.062	7.283ValLeu: 7.283 ± 0.091	1.766ValMet: 1.766 ± 0.045	2.301ValAsn: 2.301 ± 0.051	3.212ValPro: 3.212 ± 0.061	2.148ValGln: 2.148 ± 0.05	4.423ValArg: 4.423 ± 0.071	3.979ValSer: 3.979 ± 0.063	3.813ValThr: 3.813 ± 0.066	5.648ValVal: 5.648 ± 0.088	0.608ValTrp: 0.608 ± 0.023	1.842ValTyr: 1.842 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	0.814TrpAla: 0.814 ± 0.028	0.156TrpCys: 0.156 ± 0.013	0.552TrpAsp: 0.552 ± 0.024	0.601TrpGlu: 0.601 ± 0.026	0.495TrpPhe: 0.495 ± 0.022	0.695TrpGly: 0.695 ± 0.029	0.259TrpHis: 0.259 ± 0.016	0.639TrpIle: 0.639 ± 0.025	0.511TrpLys: 0.511 ± 0.023	1.338TrpLeu: 1.338 ± 0.041	0.303TrpMet: 0.303 ± 0.018	0.425TrpAsn: 0.425 ± 0.022	0.509TrpPro: 0.509 ± 0.024	0.639TrpGln: 0.639 ± 0.022	0.877TrpArg: 0.877 ± 0.034	0.599TrpSer: 0.599 ± 0.024	0.492TrpThr: 0.492 ± 0.024	0.648TrpVal: 0.648 ± 0.028	0.154TrpTrp: 0.154 ± 0.012	0.314TrpTyr: 0.314 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.309TyrAla: 2.309 ± 0.049	0.441TyrCys: 0.441 ± 0.021	1.617TyrAsp: 1.617 ± 0.047	1.418TyrGlu: 1.418 ± 0.039	1.229TyrPhe: 1.229 ± 0.036	2.194TyrGly: 2.194 ± 0.053	0.671TyrHis: 0.671 ± 0.026	1.514TyrIle: 1.514 ± 0.036	0.88TyrLys: 0.88 ± 0.031	3.096TyrLeu: 3.096 ± 0.055	0.556TyrMet: 0.556 ± 0.024	0.869TyrAsn: 0.869 ± 0.029	1.375TyrPro: 1.375 ± 0.041	1.073TyrGln: 1.073 ± 0.035	2.213TyrArg: 2.213 ± 0.05	1.627TyrSer: 1.627 ± 0.042	1.353TyrThr: 1.353 ± 0.037	1.554TyrVal: 1.554 ± 0.041	0.32TyrTrp: 0.32 ± 0.02	0.924TyrTyr: 0.924 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.002XaaSer: 0.002 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.017XaaXaa: 0.017 ± 0.012

Statistics based on 3201 proteins (984164 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---