Amino acid dipepetide frequency for Microbacterium phage Theresita

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.382AlaAla: 9.382 ± 1.155
0.631AlaCys: 0.631 ± 0.286
5.046AlaAsp: 5.046 ± 0.682
5.676AlaGlu: 5.676 ± 0.695
2.286AlaPhe: 2.286 ± 0.454
8.83AlaGly: 8.83 ± 1.116
2.05AlaHis: 2.05 ± 0.402
5.361AlaIle: 5.361 ± 0.945
4.573AlaLys: 4.573 ± 0.727
10.564AlaLeu: 10.564 ± 1.424
2.286AlaMet: 2.286 ± 0.373
3.232AlaAsn: 3.232 ± 0.519
3.311AlaPro: 3.311 ± 0.531
4.336AlaGln: 4.336 ± 0.492
6.386AlaArg: 6.386 ± 0.779
5.282AlaSer: 5.282 ± 0.739
5.992AlaThr: 5.992 ± 0.672
7.253AlaVal: 7.253 ± 0.877
2.208AlaTrp: 2.208 ± 0.438
2.523AlaTyr: 2.523 ± 0.398
0.0AlaXaa: 0.0 ± 0.0
Cys
0.237CysAla: 0.237 ± 0.123
0.079CysCys: 0.079 ± 0.089
0.631CysAsp: 0.631 ± 0.181
0.158CysGlu: 0.158 ± 0.098
0.079CysPhe: 0.079 ± 0.07
1.261CysGly: 1.261 ± 0.427
0.079CysHis: 0.079 ± 0.074
0.0CysIle: 0.0 ± 0.0
0.315CysLys: 0.315 ± 0.144
0.394CysLeu: 0.394 ± 0.203
0.079CysMet: 0.079 ± 0.079
0.158CysAsn: 0.158 ± 0.179
0.71CysPro: 0.71 ± 0.268
0.079CysGln: 0.079 ± 0.075
0.552CysArg: 0.552 ± 0.25
0.315CysSer: 0.315 ± 0.141
0.394CysThr: 0.394 ± 0.214
0.315CysVal: 0.315 ± 0.196
0.237CysTrp: 0.237 ± 0.125
0.237CysTyr: 0.237 ± 0.127
0.0CysXaa: 0.0 ± 0.0
Asp
5.282AspAla: 5.282 ± 0.579
0.631AspCys: 0.631 ± 0.216
4.021AspAsp: 4.021 ± 0.641
4.178AspGlu: 4.178 ± 0.636
1.656AspPhe: 1.656 ± 0.346
5.44AspGly: 5.44 ± 0.61
1.104AspHis: 1.104 ± 0.28
3.469AspIle: 3.469 ± 0.484
2.286AspLys: 2.286 ± 0.447
7.569AspLeu: 7.569 ± 0.646
2.05AspMet: 2.05 ± 0.421
1.025AspAsn: 1.025 ± 0.303
4.178AspPro: 4.178 ± 0.675
2.917AspGln: 2.917 ± 0.556
3.863AspArg: 3.863 ± 0.691
3.705AspSer: 3.705 ± 0.632
3.784AspThr: 3.784 ± 0.51
4.415AspVal: 4.415 ± 0.449
1.577AspTrp: 1.577 ± 0.446
2.759AspTyr: 2.759 ± 0.369
0.0AspXaa: 0.0 ± 0.0
Glu
7.096GluAla: 7.096 ± 0.722
0.237GluCys: 0.237 ± 0.129
4.257GluAsp: 4.257 ± 0.568
3.627GluGlu: 3.627 ± 0.613
1.498GluPhe: 1.498 ± 0.414
5.046GluGly: 5.046 ± 0.717
1.577GluHis: 1.577 ± 0.442
3.311GluIle: 3.311 ± 0.531
2.444GluLys: 2.444 ± 0.576
5.282GluLeu: 5.282 ± 0.826
1.261GluMet: 1.261 ± 0.302
2.129GluAsn: 2.129 ± 0.344
2.365GluPro: 2.365 ± 0.407
2.602GluGln: 2.602 ± 0.528
4.652GluArg: 4.652 ± 0.696
3.075GluSer: 3.075 ± 0.471
3.784GluThr: 3.784 ± 0.517
4.652GluVal: 4.652 ± 0.672
1.813GluTrp: 1.813 ± 0.497
1.419GluTyr: 1.419 ± 0.343
0.0GluXaa: 0.0 ± 0.0
Phe
2.05PheAla: 2.05 ± 0.344
0.158PheCys: 0.158 ± 0.115
2.286PheAsp: 2.286 ± 0.458
1.892PheGlu: 1.892 ± 0.374
0.315PhePhe: 0.315 ± 0.151
2.444PheGly: 2.444 ± 0.472
0.394PheHis: 0.394 ± 0.168
0.315PheIle: 0.315 ± 0.154
1.577PheLys: 1.577 ± 0.318
2.05PheLeu: 2.05 ± 0.37
0.394PheMet: 0.394 ± 0.18
1.577PheAsn: 1.577 ± 0.333
1.261PhePro: 1.261 ± 0.297
0.631PheGln: 0.631 ± 0.214
1.577PheArg: 1.577 ± 0.311
1.813PheSer: 1.813 ± 0.462
2.602PheThr: 2.602 ± 0.5
1.892PheVal: 1.892 ± 0.419
0.473PheTrp: 0.473 ± 0.231
0.473PheTyr: 0.473 ± 0.202
0.0PheXaa: 0.0 ± 0.0
Gly
7.569GlyAla: 7.569 ± 0.818
0.788GlyCys: 0.788 ± 0.241
5.44GlyAsp: 5.44 ± 0.56
6.465GlyGlu: 6.465 ± 0.98
2.365GlyPhe: 2.365 ± 0.372
7.411GlyGly: 7.411 ± 1.22
1.183GlyHis: 1.183 ± 0.434
5.203GlyIle: 5.203 ± 1.186
3.39GlyLys: 3.39 ± 0.51
7.884GlyLeu: 7.884 ± 1.059
1.813GlyMet: 1.813 ± 0.342
2.917GlyAsn: 2.917 ± 0.573
2.917GlyPro: 2.917 ± 0.391
4.573GlyGln: 4.573 ± 0.668
5.834GlyArg: 5.834 ± 0.643
5.676GlySer: 5.676 ± 0.684
7.411GlyThr: 7.411 ± 1.009
6.623GlyVal: 6.623 ± 0.619
2.444GlyTrp: 2.444 ± 0.599
2.838GlyTyr: 2.838 ± 0.529
0.0GlyXaa: 0.0 ± 0.0
His
1.104HisAla: 1.104 ± 0.358
0.079HisCys: 0.079 ± 0.07
1.025HisAsp: 1.025 ± 0.305
1.025HisGlu: 1.025 ± 0.264
0.315HisPhe: 0.315 ± 0.171
1.498HisGly: 1.498 ± 0.36
0.158HisHis: 0.158 ± 0.1
0.71HisIle: 0.71 ± 0.269
1.261HisLys: 1.261 ± 0.296
1.34HisLeu: 1.34 ± 0.371
0.237HisMet: 0.237 ± 0.125
0.631HisAsn: 0.631 ± 0.222
0.788HisPro: 0.788 ± 0.256
0.473HisGln: 0.473 ± 0.185
0.631HisArg: 0.631 ± 0.189
1.498HisSer: 1.498 ± 0.291
0.631HisThr: 0.631 ± 0.245
1.025HisVal: 1.025 ± 0.319
0.394HisTrp: 0.394 ± 0.139
1.025HisTyr: 1.025 ± 0.292
0.0HisXaa: 0.0 ± 0.0
Ile
3.942IleAla: 3.942 ± 0.47
0.158IleCys: 0.158 ± 0.121
4.888IleAsp: 4.888 ± 0.57
2.838IleGlu: 2.838 ± 0.446
0.552IlePhe: 0.552 ± 0.254
4.494IleGly: 4.494 ± 1.287
1.104IleHis: 1.104 ± 0.311
1.971IleIle: 1.971 ± 0.668
1.34IleLys: 1.34 ± 0.331
3.232IleLeu: 3.232 ± 0.455
0.867IleMet: 0.867 ± 0.233
1.892IleAsn: 1.892 ± 0.45
2.444IlePro: 2.444 ± 0.635
2.602IleGln: 2.602 ± 0.572
3.784IleArg: 3.784 ± 0.578
3.311IleSer: 3.311 ± 0.842
2.917IleThr: 2.917 ± 0.735
3.311IleVal: 3.311 ± 0.708
1.025IleTrp: 1.025 ± 0.34
1.577IleTyr: 1.577 ± 0.324
0.0IleXaa: 0.0 ± 0.0
Lys
3.627LysAla: 3.627 ± 0.622
0.158LysCys: 0.158 ± 0.113
1.734LysAsp: 1.734 ± 0.442
2.444LysGlu: 2.444 ± 0.464
1.34LysPhe: 1.34 ± 0.251
4.415LysGly: 4.415 ± 0.654
1.183LysHis: 1.183 ± 0.303
2.208LysIle: 2.208 ± 0.369
1.498LysLys: 1.498 ± 0.344
3.311LysLeu: 3.311 ± 0.441
1.577LysMet: 1.577 ± 0.318
1.025LysAsn: 1.025 ± 0.246
3.075LysPro: 3.075 ± 0.669
0.946LysGln: 0.946 ± 0.234
2.996LysArg: 2.996 ± 0.601
1.813LysSer: 1.813 ± 0.412
2.759LysThr: 2.759 ± 0.429
4.1LysVal: 4.1 ± 0.764
0.631LysTrp: 0.631 ± 0.185
0.71LysTyr: 0.71 ± 0.237
0.0LysXaa: 0.0 ± 0.0
Leu
9.224LeuAla: 9.224 ± 0.957
0.631LeuCys: 0.631 ± 0.283
5.913LeuAsp: 5.913 ± 0.69
4.888LeuGlu: 4.888 ± 0.616
2.286LeuPhe: 2.286 ± 0.392
6.78LeuGly: 6.78 ± 0.768
0.867LeuHis: 0.867 ± 0.279
4.336LeuIle: 4.336 ± 1.34
3.39LeuLys: 3.39 ± 0.641
6.938LeuLeu: 6.938 ± 0.819
1.577LeuMet: 1.577 ± 0.303
2.05LeuAsn: 2.05 ± 0.451
4.967LeuPro: 4.967 ± 0.796
2.602LeuGln: 2.602 ± 0.349
4.021LeuArg: 4.021 ± 0.569
5.125LeuSer: 5.125 ± 0.55
5.282LeuThr: 5.282 ± 0.794
8.12LeuVal: 8.12 ± 1.028
1.498LeuTrp: 1.498 ± 0.51
2.05LeuTyr: 2.05 ± 0.408
0.0LeuXaa: 0.0 ± 0.0
Met
3.705MetAla: 3.705 ± 0.41
0.079MetCys: 0.079 ± 0.074
1.656MetAsp: 1.656 ± 0.301
0.867MetGlu: 0.867 ± 0.209
0.237MetPhe: 0.237 ± 0.133
2.208MetGly: 2.208 ± 0.472
0.394MetHis: 0.394 ± 0.182
0.867MetIle: 0.867 ± 0.252
1.183MetLys: 1.183 ± 0.289
1.656MetLeu: 1.656 ± 0.356
0.394MetMet: 0.394 ± 0.128
0.946MetAsn: 0.946 ± 0.283
1.34MetPro: 1.34 ± 0.31
0.473MetGln: 0.473 ± 0.177
1.104MetArg: 1.104 ± 0.253
1.577MetSer: 1.577 ± 0.578
1.498MetThr: 1.498 ± 0.315
1.656MetVal: 1.656 ± 0.327
0.315MetTrp: 0.315 ± 0.141
0.394MetTyr: 0.394 ± 0.202
0.0MetXaa: 0.0 ± 0.0
Asn
3.469AsnAla: 3.469 ± 0.603
0.237AsnCys: 0.237 ± 0.163
1.734AsnAsp: 1.734 ± 0.502
1.892AsnGlu: 1.892 ± 0.396
0.394AsnPhe: 0.394 ± 0.169
3.39AsnGly: 3.39 ± 0.566
0.473AsnHis: 0.473 ± 0.192
1.892AsnIle: 1.892 ± 0.614
1.34AsnLys: 1.34 ± 0.285
2.05AsnLeu: 2.05 ± 0.496
0.552AsnMet: 0.552 ± 0.275
0.552AsnAsn: 0.552 ± 0.185
2.681AsnPro: 2.681 ± 0.451
1.498AsnGln: 1.498 ± 0.302
1.971AsnArg: 1.971 ± 0.445
2.05AsnSer: 2.05 ± 0.351
1.813AsnThr: 1.813 ± 0.423
1.971AsnVal: 1.971 ± 0.381
0.631AsnTrp: 0.631 ± 0.208
1.183AsnTyr: 1.183 ± 0.318
0.0AsnXaa: 0.0 ± 0.0
Pro
3.942ProAla: 3.942 ± 0.609
0.237ProCys: 0.237 ± 0.185
3.705ProAsp: 3.705 ± 0.633
3.863ProGlu: 3.863 ± 0.776
1.183ProPhe: 1.183 ± 0.389
5.282ProGly: 5.282 ± 0.795
0.867ProHis: 0.867 ± 0.235
1.734ProIle: 1.734 ± 0.387
1.892ProLys: 1.892 ± 0.352
3.154ProLeu: 3.154 ± 0.493
1.261ProMet: 1.261 ± 0.316
1.971ProAsn: 1.971 ± 0.427
1.656ProPro: 1.656 ± 0.341
1.656ProGln: 1.656 ± 0.336
2.286ProArg: 2.286 ± 0.437
3.627ProSer: 3.627 ± 0.647
3.39ProThr: 3.39 ± 0.545
4.1ProVal: 4.1 ± 0.531
0.867ProTrp: 0.867 ± 0.247
1.183ProTyr: 1.183 ± 0.289
0.0ProXaa: 0.0 ± 0.0
Gln
5.44GlnAla: 5.44 ± 0.701
0.079GlnCys: 0.079 ± 0.07
2.286GlnAsp: 2.286 ± 0.36
2.444GlnGlu: 2.444 ± 0.431
0.946GlnPhe: 0.946 ± 0.367
2.996GlnGly: 2.996 ± 0.594
0.473GlnHis: 0.473 ± 0.156
1.577GlnIle: 1.577 ± 0.335
1.577GlnLys: 1.577 ± 0.464
2.759GlnLeu: 2.759 ± 0.486
0.552GlnMet: 0.552 ± 0.213
2.444GlnAsn: 2.444 ± 0.504
1.183GlnPro: 1.183 ± 0.267
1.261GlnGln: 1.261 ± 0.267
2.523GlnArg: 2.523 ± 0.472
2.602GlnSer: 2.602 ± 0.534
3.154GlnThr: 3.154 ± 0.461
3.311GlnVal: 3.311 ± 0.686
1.183GlnTrp: 1.183 ± 0.302
1.261GlnTyr: 1.261 ± 0.319
0.0GlnXaa: 0.0 ± 0.0
Arg
6.071ArgAla: 6.071 ± 0.721
0.473ArgCys: 0.473 ± 0.194
4.652ArgAsp: 4.652 ± 0.528
3.232ArgGlu: 3.232 ± 0.448
2.286ArgPhe: 2.286 ± 0.437
4.809ArgGly: 4.809 ± 0.778
0.788ArgHis: 0.788 ± 0.222
2.838ArgIle: 2.838 ± 0.537
3.075ArgLys: 3.075 ± 0.578
5.125ArgLeu: 5.125 ± 0.66
2.444ArgMet: 2.444 ± 0.356
1.734ArgAsn: 1.734 ± 0.374
2.523ArgPro: 2.523 ± 0.421
2.602ArgGln: 2.602 ± 0.464
3.863ArgArg: 3.863 ± 0.713
3.469ArgSer: 3.469 ± 0.617
3.469ArgThr: 3.469 ± 0.676
5.361ArgVal: 5.361 ± 0.778
1.498ArgTrp: 1.498 ± 0.487
1.892ArgTyr: 1.892 ± 0.411
0.0ArgXaa: 0.0 ± 0.0
Ser
5.834SerAla: 5.834 ± 0.853
0.473SerCys: 0.473 ± 0.186
3.784SerAsp: 3.784 ± 0.652
3.784SerGlu: 3.784 ± 0.567
2.444SerPhe: 2.444 ± 0.415
6.544SerGly: 6.544 ± 0.771
0.473SerHis: 0.473 ± 0.181
3.232SerIle: 3.232 ± 0.592
2.444SerLys: 2.444 ± 0.444
4.573SerLeu: 4.573 ± 0.682
0.788SerMet: 0.788 ± 0.191
1.813SerAsn: 1.813 ± 0.487
2.129SerPro: 2.129 ± 0.417
2.444SerGln: 2.444 ± 0.376
4.73SerArg: 4.73 ± 0.588
3.232SerSer: 3.232 ± 0.414
3.784SerThr: 3.784 ± 0.571
4.257SerVal: 4.257 ± 0.554
1.734SerTrp: 1.734 ± 0.364
1.577SerTyr: 1.577 ± 0.285
0.0SerXaa: 0.0 ± 0.0
Thr
5.676ThrAla: 5.676 ± 0.84
0.394ThrCys: 0.394 ± 0.161
3.469ThrAsp: 3.469 ± 0.569
4.336ThrGlu: 4.336 ± 0.735
2.129ThrPhe: 2.129 ± 0.413
6.228ThrGly: 6.228 ± 0.581
0.71ThrHis: 0.71 ± 0.26
2.602ThrIle: 2.602 ± 0.538
2.917ThrLys: 2.917 ± 0.595
5.676ThrLeu: 5.676 ± 0.657
1.577ThrMet: 1.577 ± 0.386
1.892ThrAsn: 1.892 ± 0.392
4.415ThrPro: 4.415 ± 0.787
3.154ThrGln: 3.154 ± 0.622
3.942ThrArg: 3.942 ± 0.533
4.1ThrSer: 4.1 ± 0.672
5.282ThrThr: 5.282 ± 0.821
5.519ThrVal: 5.519 ± 0.752
1.498ThrTrp: 1.498 ± 0.347
1.734ThrTyr: 1.734 ± 0.45
0.0ThrXaa: 0.0 ± 0.0
Val
7.963ValAla: 7.963 ± 0.665
0.237ValCys: 0.237 ± 0.133
5.519ValAsp: 5.519 ± 0.766
5.361ValGlu: 5.361 ± 0.639
1.577ValPhe: 1.577 ± 0.376
7.096ValGly: 7.096 ± 0.896
1.104ValHis: 1.104 ± 0.288
4.257ValIle: 4.257 ± 0.702
3.232ValLys: 3.232 ± 0.522
5.676ValLeu: 5.676 ± 0.69
1.892ValMet: 1.892 ± 0.406
2.365ValAsn: 2.365 ± 0.571
3.627ValPro: 3.627 ± 0.518
3.548ValGln: 3.548 ± 0.481
4.257ValArg: 4.257 ± 0.654
4.573ValSer: 4.573 ± 0.682
5.519ValThr: 5.519 ± 0.607
7.569ValVal: 7.569 ± 0.926
2.286ValTrp: 2.286 ± 0.535
1.892ValTyr: 1.892 ± 0.425
0.0ValXaa: 0.0 ± 0.0
Trp
2.759TrpAla: 2.759 ± 0.482
0.079TrpCys: 0.079 ± 0.094
1.813TrpAsp: 1.813 ± 0.493
1.183TrpGlu: 1.183 ± 0.311
1.261TrpPhe: 1.261 ± 0.335
1.656TrpGly: 1.656 ± 0.429
0.473TrpHis: 0.473 ± 0.174
0.867TrpIle: 0.867 ± 0.242
0.71TrpLys: 0.71 ± 0.237
1.577TrpLeu: 1.577 ± 0.362
0.394TrpMet: 0.394 ± 0.153
0.946TrpAsn: 0.946 ± 0.212
0.71TrpPro: 0.71 ± 0.226
0.867TrpGln: 0.867 ± 0.295
1.498TrpArg: 1.498 ± 0.377
1.261TrpSer: 1.261 ± 0.345
1.892TrpThr: 1.892 ± 0.396
1.971TrpVal: 1.971 ± 0.407
0.71TrpTrp: 0.71 ± 0.254
1.025TrpTyr: 1.025 ± 0.304
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.838TyrAla: 2.838 ± 0.46
0.473TyrCys: 0.473 ± 0.163
2.05TyrAsp: 2.05 ± 0.418
1.971TyrGlu: 1.971 ± 0.449
1.025TyrPhe: 1.025 ± 0.259
2.917TyrGly: 2.917 ± 0.402
0.394TyrHis: 0.394 ± 0.156
1.498TyrIle: 1.498 ± 0.415
1.104TyrLys: 1.104 ± 0.341
1.813TyrLeu: 1.813 ± 0.341
0.473TyrMet: 0.473 ± 0.244
0.473TyrAsn: 0.473 ± 0.24
1.577TyrPro: 1.577 ± 0.347
0.788TyrGln: 0.788 ± 0.206
1.734TyrArg: 1.734 ± 0.302
1.971TyrSer: 1.971 ± 0.416
1.892TyrThr: 1.892 ± 0.404
2.129TyrVal: 2.129 ± 0.451
0.631TyrTrp: 0.631 ± 0.209
0.631TyrTyr: 0.631 ± 0.256
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (12685 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski