Tortoise microvirus 72

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8WA50|A0A4P8WA50_9VIRU Uncharacterized protein OS=Tortoise microvirus 72 OX=2583179 PE=4 SV=1
MM1 pKa = 7.46AVYY4 pKa = 9.91FSGTGNGGARR14 pKa = 11.84TYY16 pKa = 10.85EE17 pKa = 4.18VGQHH21 pKa = 5.12YY22 pKa = 11.18SNGNGTYY29 pKa = 10.1RR30 pKa = 11.84ANEE33 pKa = 4.39DD34 pKa = 3.33GSFTKK39 pKa = 10.61LGNVVGTTDD48 pKa = 3.14GGRR51 pKa = 11.84VVRR54 pKa = 11.84DD55 pKa = 3.39NTGGQTLQGSAGNPDD70 pKa = 3.52VRR72 pKa = 11.84WYY74 pKa = 9.76ATGNEE79 pKa = 4.18SLASGRR85 pKa = 11.84YY86 pKa = 8.78SPGTAVSLTSASGGHH101 pKa = 6.04NPPPVATPQRR111 pKa = 11.84AGGSGGGGAAGVAAGSGGAVPMANPYY137 pKa = 10.09AIPGRR142 pKa = 11.84GAAAAWSPVRR152 pKa = 11.84DD153 pKa = 3.95AAWSGDD159 pKa = 3.76DD160 pKa = 6.33DD161 pKa = 4.22PAQDD165 pKa = 3.31LRR167 pKa = 11.84MAGFHH172 pKa = 5.09WVSRR176 pKa = 11.84RR177 pKa = 11.84DD178 pKa = 3.35SSNAEE183 pKa = 3.83LAEE186 pKa = 3.8IRR188 pKa = 11.84YY189 pKa = 9.9SDD191 pKa = 2.95IGGNIVGLGVLGLDD205 pKa = 3.7IGDD208 pKa = 3.53NAARR212 pKa = 11.84LMGTRR217 pKa = 11.84FEE219 pKa = 4.52GAGIPRR225 pKa = 11.84GPEE228 pKa = 3.49LAAHH232 pKa = 6.96RR233 pKa = 11.84LSVVSEE239 pKa = 4.05YY240 pKa = 11.72ANAGVDD246 pKa = 3.34AGVAALRR253 pKa = 11.84SAIPADD259 pKa = 3.76VTEE262 pKa = 4.33TRR264 pKa = 11.84HH265 pKa = 6.12DD266 pKa = 3.33GWVSFDD272 pKa = 5.22GGQSWQYY279 pKa = 11.34DD280 pKa = 3.52GTGPAFASDD289 pKa = 3.56PKK291 pKa = 10.59RR292 pKa = 11.84NQGHH296 pKa = 6.06WADD299 pKa = 3.47IVFEE303 pKa = 4.3GTNN306 pKa = 3.13

Molecular weight: 31.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W7P5|A0A4P8W7P5_9VIRU Peptidase_M15_4 domain-containing protein OS=Tortoise microvirus 72 OX=2583179 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.42LVLRR6 pKa = 11.84TQGTLYY12 pKa = 11.12GEE14 pKa = 4.62TARR17 pKa = 11.84QRR19 pKa = 11.84AALMGLYY26 pKa = 10.16LSSLARR32 pKa = 11.84TRR34 pKa = 11.84EE35 pKa = 3.91RR36 pKa = 11.84GSWLVWPTPVEE47 pKa = 4.15EE48 pKa = 4.06ATGWISRR55 pKa = 11.84DD56 pKa = 2.81LARR59 pKa = 11.84YY60 pKa = 9.0LNRR63 pKa = 11.84ATRR66 pKa = 11.84SLLL69 pKa = 3.63

Molecular weight: 7.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1729

69

533

288.2

32.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.832 ± 1.068

0.463 ± 0.261

5.147 ± 0.334

6.767 ± 0.91

3.412 ± 0.316

9.08 ± 1.639

2.082 ± 0.419

4.338 ± 0.438

4.685 ± 1.1

6.767 ± 0.714

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.909 ± 0.298

3.875 ± 0.671

6.015 ± 0.53

4.453 ± 0.698

7.403 ± 0.726

4.8 ± 0.615

5.263 ± 0.753

7.403 ± 1.248

2.313 ± 0.448

3.991 ± 0.623
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski