Coffea arabica (Arabian coffee)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Gentianales; Rubiaceae; Ixoroideae; Gardenieae complex; Bertiereae - Coffeeae clade; Coffeeae;

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53197 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P6WKX8|A0A6P6WKX8_COFAR uncharacterized protein LOC113734027 isoform X1 OS=Coffea arabica OX=13443 GN=LOC113734027 PE=4 SV=1
MM1 pKa = 6.99EE2 pKa = 5.35TNPHH6 pKa = 5.93TNSSPEE12 pKa = 3.85PATFPSQGEE21 pKa = 3.95EE22 pKa = 4.26SPDD25 pKa = 3.38QEE27 pKa = 4.69LPGLGHH33 pKa = 6.5VSFPPSEE40 pKa = 4.29AFSLPDD46 pKa = 3.38SDD48 pKa = 5.1SFPPSEE54 pKa = 4.1SDD56 pKa = 3.5EE57 pKa = 4.49PSSEE61 pKa = 4.15PFSLPDD67 pKa = 3.75SDD69 pKa = 5.02SLPPSEE75 pKa = 4.7SDD77 pKa = 3.54EE78 pKa = 4.45PSSEE82 pKa = 4.15PFSLPDD88 pKa = 3.75SDD90 pKa = 5.02SLPPSEE96 pKa = 5.25SDD98 pKa = 3.16EE99 pKa = 4.24TTEE102 pKa = 5.0AYY104 pKa = 10.16VPSYY108 pKa = 10.8FGGDD112 pKa = 3.34SPLPSSAYY120 pKa = 9.76VDD122 pKa = 3.68SDD124 pKa = 3.19IKK126 pKa = 10.78KK127 pKa = 9.46ICDD130 pKa = 3.4STDD133 pKa = 3.35YY134 pKa = 11.02PSLCLSTIVPSLNGQTDD151 pKa = 3.66VFSVLEE157 pKa = 3.91IAIKK161 pKa = 10.38ASHH164 pKa = 6.86GYY166 pKa = 10.87ASTAFSMVKK175 pKa = 9.83KK176 pKa = 10.39LAMTPGMPNQLVAIINDD193 pKa = 3.62CRR195 pKa = 11.84DD196 pKa = 3.26SYY198 pKa = 12.23DD199 pKa = 3.42DD200 pKa = 4.06VLYY203 pKa = 11.02NFQKK207 pKa = 11.23AMTALPARR215 pKa = 11.84DD216 pKa = 3.8VGTMNTMLSAVLTDD230 pKa = 4.92VGDD233 pKa = 4.09CQDD236 pKa = 4.93AINAAKK242 pKa = 9.99IPCPLSVFGDD252 pKa = 3.48KK253 pKa = 9.2LTNMTSNCLAIASMIQQ269 pKa = 2.85

Molecular weight: 28.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P6T073|A0A6P6T073_COFAR selT-like protein OS=Coffea arabica OX=13443 GN=LOC113696143 PE=4 SV=1
MM1 pKa = 7.87PSHH4 pKa = 6.13KK5 pKa = 9.68TFFIKK10 pKa = 10.43KK11 pKa = 9.39KK12 pKa = 8.88LAKK15 pKa = 9.78KK16 pKa = 9.85KK17 pKa = 9.71RR18 pKa = 11.84QNRR21 pKa = 11.84PIPHH25 pKa = 7.85WIRR28 pKa = 11.84MRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.47RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.81LGFF51 pKa = 3.81

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42253

10944

53197

25389828

37

5382

477.3

53.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.744 ± 0.008

1.92 ± 0.005

5.306 ± 0.007

6.509 ± 0.012

4.239 ± 0.007

6.443 ± 0.011

2.412 ± 0.004

5.393 ± 0.007

6.088 ± 0.011

10.021 ± 0.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.367 ± 0.004

4.427 ± 0.006

4.848 ± 0.01

3.832 ± 0.007

5.376 ± 0.009

8.813 ± 0.012

4.719 ± 0.005

6.405 ± 0.007

1.318 ± 0.004

2.813 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski