Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; Tremellales; Cryptococcaceae; Cryptococcus; Cryptococcus neoformans species complex; Cryptococcus neoformans; Cryptococcus neoformans var. neoformans

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6746 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2M5N0|A0A0S2M5N0_CRYNJ Uncharacterized protein OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=CNF02385 PE=4 SV=1
MM1 pKa = 7.04TLKK4 pKa = 10.16IQRR7 pKa = 11.84RR8 pKa = 11.84KK9 pKa = 8.77LTAIQKK15 pKa = 7.96MISVKK20 pKa = 7.62QTIPFGLLSCLALLGSIKK38 pKa = 10.76ADD40 pKa = 3.08DD41 pKa = 4.01STNNTSWPDD50 pKa = 3.23WVTNDD55 pKa = 3.36YY56 pKa = 11.43DD57 pKa = 4.33CVIGCLSGFNDD68 pKa = 5.0TITSVPQDD76 pKa = 3.65ALAQQAFDD84 pKa = 4.5CTSSTCTGDD93 pKa = 2.92ATGNYY98 pKa = 8.4YY99 pKa = 8.24QTFYY103 pKa = 11.13YY104 pKa = 9.84IQLFYY109 pKa = 10.54ATGSIYY115 pKa = 10.36EE116 pKa = 4.51SSDD119 pKa = 3.46SAPDD123 pKa = 4.31GYY125 pKa = 11.13KK126 pKa = 10.09HH127 pKa = 6.9AAFTDD132 pKa = 3.93STDD135 pKa = 3.57TTSEE139 pKa = 4.03AAVSGDD145 pKa = 3.03WGDD148 pKa = 4.85DD149 pKa = 3.44DD150 pKa = 5.34TVDD153 pKa = 3.6PTEE156 pKa = 5.77DD157 pKa = 2.97IAASDD162 pKa = 3.89SASATDD168 pKa = 3.48SAEE171 pKa = 4.25TTGSADD177 pKa = 3.52GVDD180 pKa = 3.92GGSATDD186 pKa = 3.63TAALAIDD193 pKa = 3.84TGTEE197 pKa = 3.5NGTVVTGTAADD208 pKa = 3.85STASGSKK215 pKa = 10.19LSSASHH221 pKa = 6.45TSSQSDD227 pKa = 3.22ASTASSSVAADD238 pKa = 3.18ISEE241 pKa = 4.08SGAFKK246 pKa = 10.55TIGGDD251 pKa = 3.16VAQRR255 pKa = 11.84IFGAVGAMVFGGLWIGLL272 pKa = 3.88

Molecular weight: 27.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2LIC9|A0A0S2LIC9_CRYNJ Uncharacterized protein OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=CNC02885 PE=4 SV=1
MM1 pKa = 7.45PRR3 pKa = 11.84LARR6 pKa = 11.84VLAVPLPPRR15 pKa = 11.84ARR17 pKa = 11.84LAPLSLPRR25 pKa = 11.84ATLSAPAPAPQSSILSSLRR44 pKa = 11.84TPTSTFVASRR54 pKa = 11.84PMALSSPPIPTHH66 pKa = 6.48ILPSLPAFSPLGALRR81 pKa = 11.84HH82 pKa = 4.92VAMGAFYY89 pKa = 10.66QPSQRR94 pKa = 11.84KK95 pKa = 8.72RR96 pKa = 11.84KK97 pKa = 8.74NKK99 pKa = 9.96HH100 pKa = 5.07GFLSRR105 pKa = 11.84LKK107 pKa = 10.47GGKK110 pKa = 9.1NGRR113 pKa = 11.84KK114 pKa = 6.94TLLRR118 pKa = 11.84RR119 pKa = 11.84LLKK122 pKa = 10.03GRR124 pKa = 11.84KK125 pKa = 8.54FLSHH129 pKa = 6.98

Molecular weight: 14.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6604

142

6746

3636027

37

4972

539.0

59.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.362 ± 0.026

1.024 ± 0.01

5.376 ± 0.018

6.614 ± 0.029

3.406 ± 0.018

7.095 ± 0.025

2.343 ± 0.012

4.798 ± 0.019

5.19 ± 0.026

8.884 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.153 ± 0.01

3.405 ± 0.013

6.682 ± 0.036

3.864 ± 0.021

5.98 ± 0.024

9.082 ± 0.037

5.796 ± 0.019

6.036 ± 0.02

1.376 ± 0.011

2.533 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski