Amino acid dipepetide frequency for Phenylobacterium sp. Root1277

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.783AlaAla: 21.783 ± 0.208	1.17AlaCys: 1.17 ± 0.033	7.651AlaAsp: 7.651 ± 0.085	8.094AlaGlu: 8.094 ± 0.083	4.997AlaPhe: 4.997 ± 0.069	12.228AlaGly: 12.228 ± 0.11	2.361AlaHis: 2.361 ± 0.044	5.799AlaIle: 5.799 ± 0.076	4.831AlaLys: 4.831 ± 0.079	14.342AlaLeu: 14.342 ± 0.143	3.741AlaMet: 3.741 ± 0.058	2.98AlaAsn: 2.98 ± 0.061	7.65AlaPro: 7.65 ± 0.111	5.154AlaGln: 5.154 ± 0.069	9.879AlaArg: 9.879 ± 0.106	6.897AlaSer: 6.897 ± 0.088	6.008AlaThr: 6.008 ± 0.071	9.571AlaVal: 9.571 ± 0.098	1.92AlaTrp: 1.92 ± 0.042	2.92AlaTyr: 2.92 ± 0.05	0.001AlaXaa: 0.001 ± 0.001
Cys	1.074CysAla: 1.074 ± 0.032	0.08CysCys: 0.08 ± 0.009	0.495CysAsp: 0.495 ± 0.018	0.404CysGlu: 0.404 ± 0.017	0.271CysPhe: 0.271 ± 0.014	0.806CysGly: 0.806 ± 0.03	0.17CysHis: 0.17 ± 0.01	0.298CysIle: 0.298 ± 0.017	0.167CysLys: 0.167 ± 0.011	0.647CysLeu: 0.647 ± 0.023	0.129CysMet: 0.129 ± 0.011	0.163CysAsn: 0.163 ± 0.01	0.381CysPro: 0.381 ± 0.017	0.204CysGln: 0.204 ± 0.014	0.471CysArg: 0.471 ± 0.023	0.38CysSer: 0.38 ± 0.017	0.339CysThr: 0.339 ± 0.018	0.58CysVal: 0.58 ± 0.021	0.091CysTrp: 0.091 ± 0.008	0.156CysTyr: 0.156 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.348AspAla: 7.348 ± 0.083	0.394AspCys: 0.394 ± 0.018	3.138AspAsp: 3.138 ± 0.062	3.514AspGlu: 3.514 ± 0.071	2.164AspPhe: 2.164 ± 0.044	5.584AspGly: 5.584 ± 0.07	1.199AspHis: 1.199 ± 0.036	2.584AspIle: 2.584 ± 0.051	1.702AspLys: 1.702 ± 0.043	6.444AspLeu: 6.444 ± 0.087	1.226AspMet: 1.226 ± 0.033	1.188AspAsn: 1.188 ± 0.037	3.769AspPro: 3.769 ± 0.052	1.911AspGln: 1.911 ± 0.038	4.234AspArg: 4.234 ± 0.06	1.949AspSer: 1.949 ± 0.038	2.264AspThr: 2.264 ± 0.041	4.353AspVal: 4.353 ± 0.063	1.02AspTrp: 1.02 ± 0.029	1.554AspTyr: 1.554 ± 0.038	0.001AspXaa: 0.001 ± 0.001
Glu	8.711GluAla: 8.711 ± 0.103	0.265GluCys: 0.265 ± 0.015	2.88GluAsp: 2.88 ± 0.058	2.895GluGlu: 2.895 ± 0.061	1.738GluPhe: 1.738 ± 0.045	4.762GluGly: 4.762 ± 0.071	1.14GluHis: 1.14 ± 0.03	3.089GluIle: 3.089 ± 0.051	1.909GluLys: 1.909 ± 0.046	5.601GluLeu: 5.601 ± 0.073	1.308GluMet: 1.308 ± 0.035	1.318GluAsn: 1.318 ± 0.035	2.925GluPro: 2.925 ± 0.056	1.998GluGln: 1.998 ± 0.043	5.12GluArg: 5.12 ± 0.065	2.177GluSer: 2.177 ± 0.042	3.376GluThr: 3.376 ± 0.056	4.193GluVal: 4.193 ± 0.056	0.664GluTrp: 0.664 ± 0.024	0.933GluTyr: 0.933 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	4.759PheAla: 4.759 ± 0.062	0.351PheCys: 0.351 ± 0.019	2.788PheAsp: 2.788 ± 0.048	2.325PheGlu: 2.325 ± 0.048	1.298PhePhe: 1.298 ± 0.04	3.673PheGly: 3.673 ± 0.051	0.674PheHis: 0.674 ± 0.025	1.512PheIle: 1.512 ± 0.033	1.073PheLys: 1.073 ± 0.028	3.036PheLeu: 3.036 ± 0.054	0.807PheMet: 0.807 ± 0.026	1.085PheAsn: 1.085 ± 0.034	1.482PhePro: 1.482 ± 0.035	1.131PheGln: 1.131 ± 0.03	2.051PheArg: 2.051 ± 0.036	2.06PheSer: 2.06 ± 0.046	1.996PheThr: 1.996 ± 0.041	2.707PheVal: 2.707 ± 0.046	0.547PheTrp: 0.547 ± 0.019	0.9PheTyr: 0.9 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	12.023GlyAla: 12.023 ± 0.114	0.776GlyCys: 0.776 ± 0.024	4.891GlyAsp: 4.891 ± 0.072	5.503GlyGlu: 5.503 ± 0.077	3.713GlyPhe: 3.713 ± 0.053	8.827GlyGly: 8.827 ± 0.117	1.667GlyHis: 1.667 ± 0.034	2.868GlyIle: 2.868 ± 0.049	3.121GlyLys: 3.121 ± 0.056	9.613GlyLeu: 9.613 ± 0.098	2.18GlyMet: 2.18 ± 0.042	1.608GlyAsn: 1.608 ± 0.052	4.239GlyPro: 4.239 ± 0.065	3.253GlyGln: 3.253 ± 0.055	6.716GlyArg: 6.716 ± 0.082	4.229GlySer: 4.229 ± 0.064	3.382GlyThr: 3.382 ± 0.061	7.872GlyVal: 7.872 ± 0.086	1.565GlyTrp: 1.565 ± 0.04	2.295GlyTyr: 2.295 ± 0.047	0.001GlyXaa: 0.001 ± 0.001
His	2.313HisAla: 2.313 ± 0.044	0.182HisCys: 0.182 ± 0.012	1.096HisAsp: 1.096 ± 0.03	1.04HisGlu: 1.04 ± 0.033	0.678HisPhe: 0.678 ± 0.023	1.755HisGly: 1.755 ± 0.041	0.463HisHis: 0.463 ± 0.023	0.739HisIle: 0.739 ± 0.026	0.432HisLys: 0.432 ± 0.019	1.801HisLeu: 1.801 ± 0.039	0.453HisMet: 0.453 ± 0.017	0.372HisAsn: 0.372 ± 0.017	1.264HisPro: 1.264 ± 0.03	0.59HisGln: 0.59 ± 0.022	1.235HisArg: 1.235 ± 0.033	0.759HisSer: 0.759 ± 0.025	0.715HisThr: 0.715 ± 0.025	1.396HisVal: 1.396 ± 0.032	0.303HisTrp: 0.303 ± 0.015	0.451HisTyr: 0.451 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	6.576IleAla: 6.576 ± 0.074	0.438IleCys: 0.438 ± 0.02	3.137IleAsp: 3.137 ± 0.05	3.183IleGlu: 3.183 ± 0.055	1.416IlePhe: 1.416 ± 0.037	4.401IleGly: 4.401 ± 0.062	0.729IleHis: 0.729 ± 0.021	1.62IleIle: 1.62 ± 0.039	1.259IleLys: 1.259 ± 0.03	3.808IleLeu: 3.808 ± 0.066	0.797IleMet: 0.797 ± 0.025	1.142IleAsn: 1.142 ± 0.029	1.954IlePro: 1.954 ± 0.037	1.13IleGln: 1.13 ± 0.03	2.669IleArg: 2.669 ± 0.051	2.479IleSer: 2.479 ± 0.048	2.331IleThr: 2.331 ± 0.047	3.543IleVal: 3.543 ± 0.057	0.548IleTrp: 0.548 ± 0.02	0.948IleTyr: 0.948 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	4.713LysAla: 4.713 ± 0.068	0.115LysCys: 0.115 ± 0.01	1.767LysAsp: 1.767 ± 0.043	1.358LysGlu: 1.358 ± 0.035	0.934LysPhe: 0.934 ± 0.029	2.811LysGly: 2.811 ± 0.056	0.516LysHis: 0.516 ± 0.023	1.601LysIle: 1.601 ± 0.044	0.981LysLys: 0.981 ± 0.038	3.287LysLeu: 3.287 ± 0.057	0.703LysMet: 0.703 ± 0.023	0.717LysAsn: 0.717 ± 0.027	2.105LysPro: 2.105 ± 0.053	0.849LysGln: 0.849 ± 0.031	2.153LysArg: 2.153 ± 0.044	1.669LysSer: 1.669 ± 0.04	1.9LysThr: 1.9 ± 0.04	2.629LysVal: 2.629 ± 0.053	0.349LysTrp: 0.349 ± 0.016	0.552LysTyr: 0.552 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	15.251LeuAla: 15.251 ± 0.14	0.738LeuCys: 0.738 ± 0.027	6.009LeuAsp: 6.009 ± 0.074	5.427LeuGlu: 5.427 ± 0.072	3.273LeuPhe: 3.273 ± 0.051	8.44LeuGly: 8.44 ± 0.084	1.72LeuHis: 1.72 ± 0.039	4.73LeuIle: 4.73 ± 0.064	3.642LeuLys: 3.642 ± 0.058	8.773LeuLeu: 8.773 ± 0.118	2.193LeuMet: 2.193 ± 0.041	2.559LeuAsn: 2.559 ± 0.047	5.176LeuPro: 5.176 ± 0.06	2.724LeuGln: 2.724 ± 0.048	6.689LeuArg: 6.689 ± 0.079	6.154LeuSer: 6.154 ± 0.076	5.903LeuThr: 5.903 ± 0.075	7.215LeuVal: 7.215 ± 0.09	1.135LeuTrp: 1.135 ± 0.029	2.024LeuTyr: 2.024 ± 0.038	0.0LeuXaa: 0.0 ± 0.0
Met	3.347MetAla: 3.347 ± 0.044	0.157MetCys: 0.157 ± 0.012	1.188MetAsp: 1.188 ± 0.03	1.072MetGlu: 1.072 ± 0.032	0.701MetPhe: 0.701 ± 0.022	1.927MetGly: 1.927 ± 0.042	0.391MetHis: 0.391 ± 0.016	1.218MetIle: 1.218 ± 0.029	0.833MetLys: 0.833 ± 0.027	2.253MetLeu: 2.253 ± 0.043	0.589MetMet: 0.589 ± 0.024	0.681MetAsn: 0.681 ± 0.022	1.224MetPro: 1.224 ± 0.032	0.677MetGln: 0.677 ± 0.022	1.641MetArg: 1.641 ± 0.034	1.662MetSer: 1.662 ± 0.034	1.738MetThr: 1.738 ± 0.032	1.592MetVal: 1.592 ± 0.043	0.226MetTrp: 0.226 ± 0.012	0.299MetTyr: 0.299 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.01AsnAla: 3.01 ± 0.057	0.193AsnCys: 0.193 ± 0.013	1.259AsnAsp: 1.259 ± 0.034	1.037AsnGlu: 1.037 ± 0.026	0.91AsnPhe: 0.91 ± 0.03	2.149AsnGly: 2.149 ± 0.05	0.417AsnHis: 0.417 ± 0.018	1.134AsnIle: 1.134 ± 0.03	0.565AsnLys: 0.565 ± 0.022	2.583AsnLeu: 2.583 ± 0.047	0.523AsnMet: 0.523 ± 0.022	0.618AsnAsn: 0.618 ± 0.023	1.731AsnPro: 1.731 ± 0.042	0.691AsnGln: 0.691 ± 0.025	1.549AsnArg: 1.549 ± 0.035	1.094AsnSer: 1.094 ± 0.039	1.144AsnThr: 1.144 ± 0.035	1.896AsnVal: 1.896 ± 0.045	0.364AsnTrp: 0.364 ± 0.018	0.667AsnTyr: 0.667 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	7.498ProAla: 7.498 ± 0.099	0.314ProCys: 0.314 ± 0.019	3.688ProAsp: 3.688 ± 0.051	3.574ProGlu: 3.574 ± 0.05	2.027ProPhe: 2.027 ± 0.046	5.027ProGly: 5.027 ± 0.068	0.968ProHis: 0.968 ± 0.025	2.284ProIle: 2.284 ± 0.037	1.882ProLys: 1.882 ± 0.05	4.776ProLeu: 4.776 ± 0.065	1.24ProMet: 1.24 ± 0.032	1.374ProAsn: 1.374 ± 0.032	3.193ProPro: 3.193 ± 0.076	1.907ProGln: 1.907 ± 0.038	3.149ProArg: 3.149 ± 0.055	2.771ProSer: 2.771 ± 0.047	2.858ProThr: 2.858 ± 0.051	4.006ProVal: 4.006 ± 0.062	0.754ProTrp: 0.754 ± 0.027	1.155ProTyr: 1.155 ± 0.03	0.001ProXaa: 0.001 ± 0.001
Gln	5.314GlnAla: 5.314 ± 0.076	0.161GlnCys: 0.161 ± 0.011	1.565GlnAsp: 1.565 ± 0.037	1.411GlnGlu: 1.411 ± 0.039	1.024GlnPhe: 1.024 ± 0.032	2.749GlnGly: 2.749 ± 0.048	0.54GlnHis: 0.54 ± 0.02	1.764GlnIle: 1.764 ± 0.039	0.925GlnLys: 0.925 ± 0.029	2.941GlnLeu: 2.941 ± 0.051	0.836GlnMet: 0.836 ± 0.025	0.729GlnAsn: 0.729 ± 0.026	1.833GlnPro: 1.833 ± 0.045	1.106GlnGln: 1.106 ± 0.035	2.336GlnArg: 2.336 ± 0.049	1.637GlnSer: 1.637 ± 0.033	1.835GlnThr: 1.835 ± 0.041	2.66GlnVal: 2.66 ± 0.042	0.369GlnTrp: 0.369 ± 0.015	0.566GlnTyr: 0.566 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	8.881ArgAla: 8.881 ± 0.095	0.4ArgCys: 0.4 ± 0.018	3.911ArgAsp: 3.911 ± 0.061	4.207ArgGlu: 4.207 ± 0.058	2.91ArgPhe: 2.91 ± 0.048	5.303ArgGly: 5.303 ± 0.072	1.341ArgHis: 1.341 ± 0.03	3.307ArgIle: 3.307 ± 0.051	2.166ArgLys: 2.166 ± 0.052	8.254ArgLeu: 8.254 ± 0.097	1.824ArgMet: 1.824 ± 0.037	1.631ArgAsn: 1.631 ± 0.038	3.974ArgPro: 3.974 ± 0.061	2.486ArgGln: 2.486 ± 0.04	5.954ArgArg: 5.954 ± 0.098	3.405ArgSer: 3.405 ± 0.057	3.559ArgThr: 3.559 ± 0.06	4.72ArgVal: 4.72 ± 0.063	1.144ArgTrp: 1.144 ± 0.034	1.692ArgTyr: 1.692 ± 0.038	0.001ArgXaa: 0.001 ± 0.001
Ser	6.599SerAla: 6.599 ± 0.089	0.333SerCys: 0.333 ± 0.018	2.865SerAsp: 2.865 ± 0.05	2.628SerGlu: 2.628 ± 0.044	2.031SerPhe: 2.031 ± 0.04	5.263SerGly: 5.263 ± 0.072	0.96SerHis: 0.96 ± 0.028	2.183SerIle: 2.183 ± 0.039	1.483SerLys: 1.483 ± 0.038	5.076SerLeu: 5.076 ± 0.078	1.228SerMet: 1.228 ± 0.032	1.224SerAsn: 1.224 ± 0.033	3.01SerPro: 3.01 ± 0.05	1.723SerGln: 1.723 ± 0.036	3.411SerArg: 3.411 ± 0.055	2.597SerSer: 2.597 ± 0.058	2.433SerThr: 2.433 ± 0.045	3.773SerVal: 3.773 ± 0.061	0.747SerTrp: 0.747 ± 0.025	1.297SerTyr: 1.297 ± 0.037	0.001SerXaa: 0.001 ± 0.001
Thr	6.547ThrAla: 6.547 ± 0.079	0.328ThrCys: 0.328 ± 0.017	2.658ThrAsp: 2.658 ± 0.056	2.429ThrGlu: 2.429 ± 0.047	2.001ThrPhe: 2.001 ± 0.036	4.99ThrGly: 4.99 ± 0.063	0.907ThrHis: 0.907 ± 0.027	2.276ThrIle: 2.276 ± 0.043	1.364ThrLys: 1.364 ± 0.04	5.514ThrLeu: 5.514 ± 0.07	0.992ThrMet: 0.992 ± 0.028	1.236ThrAsn: 1.236 ± 0.036	3.754ThrPro: 3.754 ± 0.055	1.498ThrGln: 1.498 ± 0.037	3.14ThrArg: 3.14 ± 0.049	2.621ThrSer: 2.621 ± 0.055	2.704ThrThr: 2.704 ± 0.056	3.731ThrVal: 3.731 ± 0.062	0.751ThrTrp: 0.751 ± 0.027	1.319ThrTyr: 1.319 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	9.926ValAla: 9.926 ± 0.105	0.613ValCys: 0.613 ± 0.021	4.248ValAsp: 4.248 ± 0.061	4.926ValGlu: 4.926 ± 0.064	2.758ValPhe: 2.758 ± 0.047	6.315ValGly: 6.315 ± 0.082	1.235ValHis: 1.235 ± 0.031	3.725ValIle: 3.725 ± 0.056	2.3ValLys: 2.3 ± 0.053	7.345ValLeu: 7.345 ± 0.08	1.854ValMet: 1.854 ± 0.039	1.92ValAsn: 1.92 ± 0.043	3.062ValPro: 3.062 ± 0.044	2.178ValGln: 2.178 ± 0.044	5.511ValArg: 5.511 ± 0.064	4.259ValSer: 4.259 ± 0.067	4.363ValThr: 4.363 ± 0.074	6.406ValVal: 6.406 ± 0.085	1.005ValTrp: 1.005 ± 0.028	1.471ValTyr: 1.471 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.575TrpAla: 1.575 ± 0.038	0.114TrpCys: 0.114 ± 0.009	0.721TrpAsp: 0.721 ± 0.023	0.641TrpGlu: 0.641 ± 0.026	0.522TrpPhe: 0.522 ± 0.02	1.037TrpGly: 1.037 ± 0.029	0.209TrpHis: 0.209 ± 0.013	0.655TrpIle: 0.655 ± 0.022	0.477TrpLys: 0.477 ± 0.022	1.59TrpLeu: 1.59 ± 0.036	0.354TrpMet: 0.354 ± 0.019	0.425TrpAsn: 0.425 ± 0.02	0.722TrpPro: 0.722 ± 0.026	0.38TrpGln: 0.38 ± 0.018	1.443TrpArg: 1.443 ± 0.036	0.948TrpSer: 0.948 ± 0.025	0.927TrpThr: 0.927 ± 0.027	0.872TrpVal: 0.872 ± 0.026	0.243TrpTrp: 0.243 ± 0.013	0.248TrpTyr: 0.248 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.73TyrAla: 2.73 ± 0.046	0.185TyrCys: 0.185 ± 0.011	1.582TyrAsp: 1.582 ± 0.036	1.334TyrGlu: 1.334 ± 0.034	0.889TyrPhe: 0.889 ± 0.027	2.297TyrGly: 2.297 ± 0.04	0.432TyrHis: 0.432 ± 0.018	0.722TyrIle: 0.722 ± 0.025	0.598TyrLys: 0.598 ± 0.025	2.092TyrLeu: 2.092 ± 0.044	0.438TyrMet: 0.438 ± 0.016	0.564TyrAsn: 0.564 ± 0.025	0.996TyrPro: 0.996 ± 0.031	0.71TyrGln: 0.71 ± 0.026	1.718TyrArg: 1.718 ± 0.041	1.117TyrSer: 1.117 ± 0.029	0.984TyrThr: 0.984 ± 0.028	1.704TyrVal: 1.704 ± 0.038	0.354TyrTrp: 0.354 ± 0.015	0.581TyrTyr: 0.581 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4022 proteins (1269598 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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