Thermoactinomyces sp. DSM 45891

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Thermoactinomycetaceae; Thermoactinomyces; unclassified Thermoactinomyces

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2915 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1K1U3D8|A0A1K1U3D8_9BACL Diadenosine tetraphosphate (Ap4A) hydrolase OS=Thermoactinomyces sp. DSM 45891 OX=1761907 GN=SAMN04487866_101425 PE=4 SV=1
MM1 pKa = 7.42YY2 pKa = 9.89DD3 pKa = 3.16RR4 pKa = 11.84LRR6 pKa = 11.84RR7 pKa = 11.84EE8 pKa = 3.58VSYY11 pKa = 11.18VQGLMEE17 pKa = 4.58GANKK21 pKa = 10.03EE22 pKa = 4.27SSHH25 pKa = 6.9PEE27 pKa = 3.26GKK29 pKa = 10.37AIQRR33 pKa = 11.84LLDD36 pKa = 3.6IVDD39 pKa = 4.17EE40 pKa = 4.11LLEE43 pKa = 4.54ANEE46 pKa = 4.1HH47 pKa = 5.95LEE49 pKa = 4.08HH50 pKa = 7.23RR51 pKa = 11.84FLEE54 pKa = 4.33LEE56 pKa = 4.42EE57 pKa = 4.12YY58 pKa = 10.82VEE60 pKa = 6.24SIDD63 pKa = 5.94DD64 pKa = 4.36DD65 pKa = 4.93LNDD68 pKa = 4.03MEE70 pKa = 5.41LLVYY74 pKa = 10.69DD75 pKa = 5.75DD76 pKa = 5.62EE77 pKa = 7.94DD78 pKa = 6.69DD79 pKa = 4.69DD80 pKa = 7.08DD81 pKa = 5.48EE82 pKa = 7.38DD83 pKa = 4.39EE84 pKa = 4.61LCIVCPEE91 pKa = 4.36CGEE94 pKa = 4.17DD95 pKa = 4.0VYY97 pKa = 11.96VDD99 pKa = 3.84YY100 pKa = 11.44EE101 pKa = 4.26DD102 pKa = 4.14VADD105 pKa = 5.27AEE107 pKa = 4.61IEE109 pKa = 4.18LLCPEE114 pKa = 4.61CHH116 pKa = 5.62TVLIVEE122 pKa = 4.64DD123 pKa = 4.38ASDD126 pKa = 3.94IEE128 pKa = 4.4PDD130 pKa = 3.21NSGVDD135 pKa = 3.3EE136 pKa = 4.25EE137 pKa = 4.79TRR139 pKa = 3.38

Molecular weight: 16.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1K2A8N4|A0A1K2A8N4_9BACL Uncharacterized protein OS=Thermoactinomyces sp. DSM 45891 OX=1761907 GN=SAMN04487866_1329 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.23KK3 pKa = 10.37KK4 pKa = 10.32IVTTQSNCPPANCSIIQRR22 pKa = 11.84IFRR25 pKa = 11.84RR26 pKa = 11.84LGALGRR32 pKa = 11.84RR33 pKa = 11.84IANLNRR39 pKa = 11.84RR40 pKa = 11.84IVALEE45 pKa = 3.66NRR47 pKa = 11.84SGLSAYY53 pKa = 9.09GQVYY57 pKa = 10.3NLSTGITLTIPAGGNVPFSNNGVLNNITHH86 pKa = 5.6TTGSADD92 pKa = 3.83VILPQAGQYY101 pKa = 9.28EE102 pKa = 4.81INFQVSAVGALLGLLAAFAVTVNGTVVPQSTFAINLTGIIGLAAQISGSVIIDD155 pKa = 3.17VPANATITLRR165 pKa = 11.84NVSGVPVPLLDD176 pKa = 3.75TLIGATTPLSNASMTLKK193 pKa = 10.81RR194 pKa = 11.84LGPVV198 pKa = 2.9

Molecular weight: 20.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2915

0

2915

868414

39

3360

297.9

33.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.645 ± 0.044

0.864 ± 0.014

5.103 ± 0.039

7.046 ± 0.05

4.178 ± 0.037

7.092 ± 0.062

2.314 ± 0.023

7.222 ± 0.049

6.175 ± 0.044

9.675 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.748 ± 0.022

3.763 ± 0.034

4.032 ± 0.031

4.468 ± 0.036

4.865 ± 0.04

6.343 ± 0.037

5.575 ± 0.046

7.245 ± 0.035

1.202 ± 0.018

3.442 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski