Pseudomonas phage KP1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Septimatrevirus; unclassified Septimatrevirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G5QAU1|A0A6G5QAU1_9CAUD Uncharacterized protein OS=Pseudomonas phage KP1 OX=2562463 PE=4 SV=1
MM1 pKa = 7.19MSDD4 pKa = 3.52YY5 pKa = 11.09DD6 pKa = 4.19DD7 pKa = 4.82KK8 pKa = 11.75EE9 pKa = 3.94EE10 pKa = 4.54SIEE13 pKa = 4.47DD14 pKa = 3.55GSPYY18 pKa = 10.23EE19 pKa = 3.76IYY21 pKa = 10.81EE22 pKa = 4.31FIGTYY27 pKa = 9.57RR28 pKa = 11.84SYY30 pKa = 12.01YY31 pKa = 6.52MTSDD35 pKa = 3.47ALSHH39 pKa = 6.79DD40 pKa = 4.5FEE42 pKa = 4.66GHH44 pKa = 6.23SYY46 pKa = 10.24TPVPGLKK53 pKa = 9.89RR54 pKa = 11.84SSLKK58 pKa = 10.92AGTHH62 pKa = 5.7EE63 pKa = 4.71EE64 pKa = 4.21DD65 pKa = 4.66NIDD68 pKa = 3.77LTVVIPITEE77 pKa = 4.29QIVKK81 pKa = 10.3DD82 pKa = 3.74YY83 pKa = 11.11GFQTTPPALDD93 pKa = 3.21LTIYY97 pKa = 10.53RR98 pKa = 11.84FQRR101 pKa = 11.84DD102 pKa = 3.31ALTFVAYY109 pKa = 9.26WKK111 pKa = 10.77GPVASITIDD120 pKa = 3.58DD121 pKa = 4.39EE122 pKa = 4.56YY123 pKa = 11.38ATLRR127 pKa = 11.84TPSKK131 pKa = 10.34FGNILQGNIPNIYY144 pKa = 9.19VQPPCNNVLFDD155 pKa = 3.68EE156 pKa = 4.74LCRR159 pKa = 11.84VSRR162 pKa = 11.84AANSLTTTVSLVEE175 pKa = 4.39GVNITIPSISPFVDD189 pKa = 3.06GWFIGGEE196 pKa = 3.83IAVPARR202 pKa = 11.84NEE204 pKa = 3.31RR205 pKa = 11.84RR206 pKa = 11.84MIIAQAGPVLTVNYY220 pKa = 9.63AFSRR224 pKa = 11.84LGPGTSIQVTAGCDD238 pKa = 3.3HH239 pKa = 7.03SFTGANGCPKK249 pKa = 10.25FNNQINYY256 pKa = 8.85GGCPYY261 pKa = 10.72VPGEE265 pKa = 4.33SNNPFTNGVRR275 pKa = 3.49

Molecular weight: 30.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G5QAI1|A0A6G5QAI1_9CAUD Uncharacterized protein OS=Pseudomonas phage KP1 OX=2562463 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.05GVLMSEE8 pKa = 5.22PINALPIPQQCDD20 pKa = 2.99ACCSLNIKK28 pKa = 10.12FLLNEE33 pKa = 3.96PVSANVYY40 pKa = 10.27FCDD43 pKa = 3.87GCGASAGCHH52 pKa = 5.88RR53 pKa = 11.84GTNKK57 pKa = 10.19PLGQMGDD64 pKa = 3.12RR65 pKa = 11.84ATRR68 pKa = 11.84QLRR71 pKa = 11.84KK72 pKa = 9.37KK73 pKa = 10.7AHH75 pKa = 6.87DD76 pKa = 3.93EE77 pKa = 3.74FDD79 pKa = 3.57KK80 pKa = 10.98LWRR83 pKa = 11.84SGLMRR88 pKa = 11.84RR89 pKa = 11.84SKK91 pKa = 10.76AYY93 pKa = 10.25NWLALQLGIEE103 pKa = 4.6PDD105 pKa = 3.74NCHH108 pKa = 6.14MSQLSKK114 pKa = 11.28DD115 pKa = 3.53QLKK118 pKa = 10.88DD119 pKa = 3.71VITLSADD126 pKa = 3.45YY127 pKa = 8.81LTNNHH132 pKa = 6.39AALARR137 pKa = 11.84RR138 pKa = 11.84KK139 pKa = 9.59AKK141 pKa = 10.12QDD143 pKa = 2.95AKK145 pKa = 10.29QEE147 pKa = 4.06KK148 pKa = 9.3RR149 pKa = 11.84NKK151 pKa = 9.62RR152 pKa = 11.84NAAAEE157 pKa = 4.01RR158 pKa = 11.84RR159 pKa = 11.84TSNDD163 pKa = 2.01ARR165 pKa = 11.84QRR167 pKa = 11.84TRR169 pKa = 11.84KK170 pKa = 9.49RR171 pKa = 3.17

Molecular weight: 19.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36

0

36

11605

109

973

322.4

35.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.521 ± 0.779

1.051 ± 0.2

5.205 ± 0.22

6.04 ± 0.372

3.852 ± 0.223

7.307 ± 0.289

1.448 ± 0.197

5.515 ± 0.211

5.816 ± 0.374

8.126 ± 0.305

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.361 ± 0.178

5.11 ± 0.225

4.791 ± 0.363

4.248 ± 0.72

4.894 ± 0.266

6.325 ± 0.307

6.127 ± 0.313

6.626 ± 0.301

1.361 ± 0.152

3.274 ± 0.274
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski