Warrego virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A097I4F1|A0A097I4F1_9REOV Non-structural protein NS3 OS=Warrego virus OX=40062 PE=3 SV=1
MM1 pKa = 7.59EE2 pKa = 5.03GKK4 pKa = 8.33KK5 pKa = 9.18TNVPRR10 pKa = 11.84LFTKK14 pKa = 10.3RR15 pKa = 11.84VYY17 pKa = 11.07VLDD20 pKa = 3.76TRR22 pKa = 11.84KK23 pKa = 8.6STICGRR29 pKa = 11.84ISQLAGLPYY38 pKa = 10.54CKK40 pKa = 9.3IEE42 pKa = 4.03SGRR45 pKa = 11.84VVSVTACAQPSDD57 pKa = 3.34RR58 pKa = 11.84GYY60 pKa = 10.83VLEE63 pKa = 4.71IEE65 pKa = 4.69GKK67 pKa = 8.21GAYY70 pKa = 9.72RR71 pKa = 11.84IVDD74 pKa = 3.85GQDD77 pKa = 3.3TISLMIDD84 pKa = 3.35DD85 pKa = 5.33YY86 pKa = 11.13GVSMTTGRR94 pKa = 11.84WEE96 pKa = 3.95EE97 pKa = 4.1YY98 pKa = 9.1AFEE101 pKa = 4.21MVSVRR106 pKa = 11.84PHH108 pKa = 5.22VMKK111 pKa = 10.89VKK113 pKa = 10.14EE114 pKa = 3.96DD115 pKa = 3.26RR116 pKa = 11.84VFKK119 pKa = 10.75NIEE122 pKa = 3.79IKK124 pKa = 10.44HH125 pKa = 5.52GRR127 pKa = 11.84GCAAVPPYY135 pKa = 10.23MRR137 pKa = 11.84VDD139 pKa = 3.78RR140 pKa = 11.84EE141 pKa = 4.22QNSEE145 pKa = 3.76LRR147 pKa = 11.84LPGTEE152 pKa = 3.86RR153 pKa = 11.84SDD155 pKa = 3.4MDD157 pKa = 3.4VSTYY161 pKa = 7.96RR162 pKa = 11.84QKK164 pKa = 10.75RR165 pKa = 11.84RR166 pKa = 11.84EE167 pKa = 3.98EE168 pKa = 4.04RR169 pKa = 11.84EE170 pKa = 3.82GMDD173 pKa = 4.08RR174 pKa = 11.84NTQRR178 pKa = 11.84LQPAHH183 pKa = 6.01MRR185 pKa = 11.84SEE187 pKa = 4.18EE188 pKa = 3.97MDD190 pKa = 3.16RR191 pKa = 11.84SSWSEE196 pKa = 3.51QVNASKK202 pKa = 10.38AQKK205 pKa = 10.29YY206 pKa = 10.17DD207 pKa = 3.25EE208 pKa = 4.82EE209 pKa = 4.46FNKK212 pKa = 10.09FQEE215 pKa = 4.4SVRR218 pKa = 11.84QIEE221 pKa = 5.08LEE223 pKa = 3.96DD224 pKa = 4.39EE225 pKa = 4.55YY226 pKa = 10.9PAGQQDD232 pKa = 5.22DD233 pKa = 5.39DD234 pKa = 4.7NDD236 pKa = 4.48DD237 pKa = 3.73NFGLGLVKK245 pKa = 10.94DD246 pKa = 4.6EE247 pKa = 6.05DD248 pKa = 3.67NTSQKK253 pKa = 10.74KK254 pKa = 10.35GGEE257 pKa = 4.02EE258 pKa = 3.93RR259 pKa = 11.84EE260 pKa = 4.43KK261 pKa = 10.9KK262 pKa = 10.45ASQQDD267 pKa = 3.7DD268 pKa = 3.9FGLDD272 pKa = 3.71FEE274 pKa = 4.88EE275 pKa = 4.9EE276 pKa = 4.32CAKK279 pKa = 10.53FNYY282 pKa = 8.79LTDD285 pKa = 3.77TYY287 pKa = 11.21SEE289 pKa = 4.18LVGKK293 pKa = 10.22IVAKK297 pKa = 10.21NLKK300 pKa = 9.26QLGGKK305 pKa = 9.65SMIFPQLGDD314 pKa = 3.51RR315 pKa = 11.84YY316 pKa = 7.78TEE318 pKa = 3.97KK319 pKa = 10.95LVVLTKK325 pKa = 10.34EE326 pKa = 4.41CNNVPLYY333 pKa = 11.06NIDD336 pKa = 4.26EE337 pKa = 4.22IAKK340 pKa = 8.86EE341 pKa = 3.93YY342 pKa = 10.7RR343 pKa = 11.84FAAIGTCKK351 pKa = 10.19RR352 pKa = 11.84IVMVAKK358 pKa = 10.43GNSFTALPAGAMM370 pKa = 3.88

Molecular weight: 42.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A097I4H0|A0A097I4H0_9REOV Core protein VP4 OS=Warrego virus OX=40062 PE=3 SV=1
MM1 pKa = 7.73LSTLVSRR8 pKa = 11.84CAEE11 pKa = 3.96EE12 pKa = 4.71KK13 pKa = 10.43EE14 pKa = 4.02EE15 pKa = 4.23SRR17 pKa = 11.84RR18 pKa = 11.84LSKK21 pKa = 10.8QFIEE25 pKa = 4.36VDD27 pKa = 3.3LTDD30 pKa = 3.47TMEE33 pKa = 5.6ADD35 pKa = 5.0DD36 pKa = 4.08MNKK39 pKa = 8.71TPSAPPVYY47 pKa = 9.3TPPNAPPNPATVSLNILTNAMSSTTGATNAQKK79 pKa = 10.66EE80 pKa = 4.28EE81 pKa = 3.88KK82 pKa = 10.06AAYY85 pKa = 9.78ASYY88 pKa = 11.15AEE90 pKa = 4.6AMKK93 pKa = 10.83DD94 pKa = 3.55DD95 pKa = 4.1INIKK99 pKa = 9.82IIKK102 pKa = 9.94SRR104 pKa = 11.84VNEE107 pKa = 3.92QIIPRR112 pKa = 11.84LEE114 pKa = 4.83HH115 pKa = 6.52EE116 pKa = 4.41LQSMQRR122 pKa = 11.84RR123 pKa = 11.84KK124 pKa = 10.25KK125 pKa = 9.65ILYY128 pKa = 9.94AVMLISAILALFTSGVTIIKK148 pKa = 9.64DD149 pKa = 3.66LKK151 pKa = 9.44ITLPNSGNATHH162 pKa = 7.77IEE164 pKa = 3.88MPAWVRR170 pKa = 11.84DD171 pKa = 3.45LSVFIGMLNFASMGVALGCAKK192 pKa = 10.21MMDD195 pKa = 3.44GMVRR199 pKa = 11.84SIDD202 pKa = 3.55QVRR205 pKa = 11.84KK206 pKa = 9.84EE207 pKa = 3.85ISKK210 pKa = 10.48KK211 pKa = 9.81KK212 pKa = 10.5AYY214 pKa = 9.62IDD216 pKa = 3.17ATRR219 pKa = 11.84IAFRR223 pKa = 11.84GSVDD227 pKa = 3.83FSKK230 pKa = 11.21LDD232 pKa = 3.36TRR234 pKa = 11.84DD235 pKa = 3.26TEE237 pKa = 4.62RR238 pKa = 11.84YY239 pKa = 9.48AVTPEE244 pKa = 3.69ARR246 pKa = 11.84GLLWW250 pKa = 4.21

Molecular weight: 27.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6212

250

1308

621.2

71.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.165 ± 0.62

1.062 ± 0.211

5.924 ± 0.207

7.679 ± 0.558

4.089 ± 0.353

5.441 ± 0.345

2.093 ± 0.236

7.308 ± 0.368

6.616 ± 0.844

8.081 ± 0.372

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.912 ± 0.327

4.572 ± 0.234

3.654 ± 0.297

3.719 ± 0.334

6.648 ± 0.331

5.602 ± 0.316

5.425 ± 0.31

6.825 ± 0.199

1.159 ± 0.17

4.024 ± 0.353
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski