Pseudomonas virus H66

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Hollowayvirus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7TP23|L7TP23_9CAUD Uncharacterized protein OS=Pseudomonas virus H66 OX=1273707 GN=H66_0031 PE=4 SV=1
MM1 pKa = 6.67MWTYY5 pKa = 11.07RR6 pKa = 11.84EE7 pKa = 3.91RR8 pKa = 11.84HH9 pKa = 5.13NRR11 pKa = 11.84AAFNCAQQAWDD22 pKa = 4.29RR23 pKa = 11.84EE24 pKa = 4.2VDD26 pKa = 4.05PLWDD30 pKa = 3.86QPDD33 pKa = 4.44PDD35 pKa = 4.45PQDD38 pKa = 3.65EE39 pKa = 4.29EE40 pKa = 4.47QEE42 pKa = 4.14NDD44 pKa = 4.79DD45 pKa = 5.3GLQQQ49 pKa = 3.72

Molecular weight: 5.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7TKP8|L7TKP8_9CAUD Uncharacterized protein OS=Pseudomonas virus H66 OX=1273707 GN=H66_004 PE=4 SV=1
MM1 pKa = 7.83TYY3 pKa = 9.83TDD5 pKa = 3.28EE6 pKa = 5.08RR7 pKa = 11.84GTFILRR13 pKa = 11.84WTRR16 pKa = 11.84RR17 pKa = 11.84LKK19 pKa = 10.69NGQIQRR25 pKa = 11.84AVGKK29 pKa = 8.8PFKK32 pKa = 10.47IYY34 pKa = 10.34ISS36 pKa = 3.57

Molecular weight: 4.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

20131

36

3787

283.5

31.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.478 ± 0.63

0.84 ± 0.193

6.16 ± 0.193

6.348 ± 0.268

3.05 ± 0.165

7.893 ± 0.307

1.972 ± 0.163

4.257 ± 0.259

4.128 ± 0.258

8.246 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.444 ± 0.109

2.966 ± 0.133

5.33 ± 0.353

5.112 ± 0.244

7.337 ± 0.305

5.559 ± 0.172

5.256 ± 0.247

6.607 ± 0.222

1.45 ± 0.145

2.568 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski