Firmicutes bacterium M10-2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; unclassified Firmicutes sensu stricto

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2256 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9LNV1|R9LNV1_9FIRM Orotate phosphoribosyltransferase OS=Firmicutes bacterium M10-2 OX=1235796 GN=pyrE PE=3 SV=1
MM1 pKa = 7.31KK2 pKa = 10.57AKK4 pKa = 10.07IALILALTLGLSACSAAPAKK24 pKa = 10.5NQTDD28 pKa = 3.7QPISQQANEE37 pKa = 4.28QFTKK41 pKa = 10.82YY42 pKa = 10.69LNDD45 pKa = 3.56YY46 pKa = 8.12VVEE49 pKa = 4.06MAEE52 pKa = 4.2SDD54 pKa = 4.17FTTMHH59 pKa = 6.19QFFEE63 pKa = 4.42HH64 pKa = 6.96PEE66 pKa = 3.91NYY68 pKa = 10.54GIDD71 pKa = 3.75PEE73 pKa = 4.37KK74 pKa = 11.36VEE76 pKa = 4.15VTLGSIEE83 pKa = 4.06PDD85 pKa = 3.36EE86 pKa = 4.2EE87 pKa = 4.49SKK89 pKa = 11.27ALEE92 pKa = 4.57KK93 pKa = 10.88KK94 pKa = 10.82LSDD97 pKa = 3.52QLDD100 pKa = 3.69SFDD103 pKa = 5.07RR104 pKa = 11.84SSLDD108 pKa = 3.15EE109 pKa = 4.34RR110 pKa = 11.84QQTIYY115 pKa = 11.09DD116 pKa = 3.51QLQYY120 pKa = 11.21QFDD123 pKa = 3.55QSQQEE128 pKa = 4.16EE129 pKa = 4.6EE130 pKa = 4.18EE131 pKa = 4.19KK132 pKa = 10.74FRR134 pKa = 11.84YY135 pKa = 8.59LGNMWSSMNGLPQTLVSYY153 pKa = 10.25FSEE156 pKa = 4.53YY157 pKa = 10.4EE158 pKa = 3.81LRR160 pKa = 11.84QEE162 pKa = 4.42SDD164 pKa = 3.01IAPLIQLINDD174 pKa = 3.6IPRR177 pKa = 11.84YY178 pKa = 7.41TKK180 pKa = 10.38EE181 pKa = 3.51ALTYY185 pKa = 9.92SEE187 pKa = 4.7KK188 pKa = 10.35QAEE191 pKa = 4.66NDD193 pKa = 3.56TLMLDD198 pKa = 3.89YY199 pKa = 10.91EE200 pKa = 4.58DD201 pKa = 5.35TIQSCQEE208 pKa = 3.6ILNSKK213 pKa = 9.34EE214 pKa = 3.72NSAVTAEE221 pKa = 4.02LMKK224 pKa = 10.82DD225 pKa = 3.72VEE227 pKa = 4.57QLDD230 pKa = 4.31LDD232 pKa = 4.03PEE234 pKa = 4.17KK235 pKa = 10.33TIQYY239 pKa = 7.71EE240 pKa = 4.19KK241 pKa = 10.47QIKK244 pKa = 9.65DD245 pKa = 3.43ALDD248 pKa = 3.28TSFFPSYY255 pKa = 11.25QLMIDD260 pKa = 4.17GLTKK264 pKa = 10.76LKK266 pKa = 10.97DD267 pKa = 3.73SIQPVAGMASLEE279 pKa = 4.15NGKK282 pKa = 9.99EE283 pKa = 4.03YY284 pKa = 11.47YY285 pKa = 10.05EE286 pKa = 5.07LLVQQYY292 pKa = 9.05TGTTDD297 pKa = 3.28SIEE300 pKa = 4.34TIKK303 pKa = 11.1KK304 pKa = 9.52NVEE307 pKa = 3.78NEE309 pKa = 3.25IDD311 pKa = 3.97EE312 pKa = 4.93ISTQVSSLLTDD323 pKa = 4.43DD324 pKa = 4.68NYY326 pKa = 11.99DD327 pKa = 3.58MILNYY332 pKa = 9.87MNLKK336 pKa = 9.15TDD338 pKa = 4.13FEE340 pKa = 4.5NVEE343 pKa = 4.71DD344 pKa = 4.21ILVFLNEE351 pKa = 3.77NYY353 pKa = 10.33DD354 pKa = 3.53RR355 pKa = 11.84YY356 pKa = 10.15FPQVDD361 pKa = 3.06AMKK364 pKa = 10.92YY365 pKa = 10.1DD366 pKa = 4.3LQPLADD372 pKa = 4.41DD373 pKa = 3.94QSQEE377 pKa = 4.14GIVAYY382 pKa = 9.38FMIPAVDD389 pKa = 3.3STTDD393 pKa = 2.92YY394 pKa = 10.82RR395 pKa = 11.84IRR397 pKa = 11.84YY398 pKa = 7.39NRR400 pKa = 11.84RR401 pKa = 11.84DD402 pKa = 3.65YY403 pKa = 11.81GDD405 pKa = 4.66DD406 pKa = 3.52PSALSLYY413 pKa = 8.57QTLAHH418 pKa = 6.44EE419 pKa = 5.71GIPGHH424 pKa = 6.54MYY426 pKa = 8.83QAQYY430 pKa = 11.03NKK432 pKa = 9.86EE433 pKa = 3.9HH434 pKa = 5.77FQYY437 pKa = 10.29TIEE440 pKa = 4.18YY441 pKa = 8.72FLSNSGFSEE450 pKa = 5.3GYY452 pKa = 7.93ATYY455 pKa = 11.03VEE457 pKa = 4.12NQSLKK462 pKa = 10.74FLDD465 pKa = 4.9LDD467 pKa = 4.17PDD469 pKa = 4.86LINMYY474 pKa = 10.29VYY476 pKa = 11.06EE477 pKa = 5.1DD478 pKa = 4.21LLNNNYY484 pKa = 9.45ILLMDD489 pKa = 4.0IAVNYY494 pKa = 9.23EE495 pKa = 3.75GLNLEE500 pKa = 4.53EE501 pKa = 4.32FQSTFDD507 pKa = 3.75MFDD510 pKa = 3.21PTALEE515 pKa = 4.27SIYY518 pKa = 10.87DD519 pKa = 3.76QLAGNPGVFMSYY531 pKa = 10.22YY532 pKa = 9.71YY533 pKa = 10.87GYY535 pKa = 11.28NKK537 pKa = 9.84ISNLRR542 pKa = 11.84EE543 pKa = 3.79EE544 pKa = 4.65AKK546 pKa = 10.49EE547 pKa = 3.76ALKK550 pKa = 10.98DD551 pKa = 3.67SFDD554 pKa = 3.89DD555 pKa = 4.23VEE557 pKa = 4.78FNNALLQSGSVNFDD571 pKa = 4.75LIQQNIDD578 pKa = 3.66KK579 pKa = 10.47YY580 pKa = 11.27IKK582 pKa = 10.43DD583 pKa = 3.71NQQ585 pKa = 3.45

Molecular weight: 67.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9LWV9|R9LWV9_9FIRM Uncharacterized protein OS=Firmicutes bacterium M10-2 OX=1235796 GN=C815_01577 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 9.71RR10 pKa = 11.84KK11 pKa = 9.45HH12 pKa = 4.84KK13 pKa = 8.72TVHH16 pKa = 5.79GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.01VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2256

0

2256

690888

28

3426

306.2

34.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.643 ± 0.051

1.403 ± 0.023

5.805 ± 0.044

7.362 ± 0.052

4.486 ± 0.042

6.231 ± 0.044

2.125 ± 0.025

8.011 ± 0.044

7.376 ± 0.043

9.298 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.079 ± 0.027

4.74 ± 0.037

3.37 ± 0.029

3.896 ± 0.032

3.897 ± 0.044

5.769 ± 0.039

5.071 ± 0.042

6.38 ± 0.045

0.982 ± 0.019

4.076 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski