Hypsizygus marmoreus (White beech mushroom) (Agaricus marmoreus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Lyophyllaceae; Hypsizygus

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16572 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A369JPG2|A0A369JPG2_HYPMA Uncharacterized protein OS=Hypsizygus marmoreus OX=39966 GN=Hypma_011511 PE=4 SV=1
MM1 pKa = 7.26SANTISEE8 pKa = 4.5NGQHH12 pKa = 5.97TLPDD16 pKa = 3.85FQEE19 pKa = 4.1RR20 pKa = 11.84LATIRR25 pKa = 11.84TRR27 pKa = 11.84SRR29 pKa = 11.84ISYY32 pKa = 8.66TPVLTTPSLTTNTQQPQHH50 pKa = 7.36PDD52 pKa = 2.84TVMSDD57 pKa = 3.36TTNLFSLARR66 pKa = 11.84SKK68 pKa = 11.19LHH70 pKa = 5.81VSVGGKK76 pKa = 9.73DD77 pKa = 3.17SCSLHH82 pKa = 6.25RR83 pKa = 11.84WVLLKK88 pKa = 10.95NSIIRR93 pKa = 11.84SPSVPSSPSASDD105 pKa = 3.79YY106 pKa = 10.89PDD108 pKa = 3.38VNSEE112 pKa = 4.14YY113 pKa = 11.03AVDD116 pKa = 4.58DD117 pKa = 4.03EE118 pKa = 5.3ADD120 pKa = 3.58EE121 pKa = 4.41EE122 pKa = 4.76EE123 pKa = 4.46EE124 pKa = 4.48VNSEE128 pKa = 4.05EE129 pKa = 4.82LDD131 pKa = 3.41SFMFPDD137 pKa = 3.68AGKK140 pKa = 10.45LVGGSTADD148 pKa = 3.44VTASEE153 pKa = 4.96AEE155 pKa = 4.1WLDD158 pKa = 3.61SLLEE162 pKa = 4.04TLGDD166 pKa = 4.13DD167 pKa = 5.07DD168 pKa = 6.11DD169 pKa = 6.95DD170 pKa = 4.46EE171 pKa = 4.84FTVEE175 pKa = 4.49SDD177 pKa = 3.25PSLSILPVDD186 pKa = 4.4EE187 pKa = 6.87DD188 pKa = 4.87DD189 pKa = 5.48DD190 pKa = 4.23QLLLSPMGSPISSSDD205 pKa = 3.69DD206 pKa = 3.78LPPLSAFYY214 pKa = 9.9PHH216 pKa = 6.63STTVSYY222 pKa = 10.59SYY224 pKa = 9.69PYY226 pKa = 9.16PVPYY230 pKa = 10.25PPFHH234 pKa = 7.18PPLIHH239 pKa = 7.42SYY241 pKa = 10.88EE242 pKa = 4.07FDD244 pKa = 3.62SDD246 pKa = 4.03FDD248 pKa = 4.42PSLSSLPAPYY258 pKa = 10.12DD259 pKa = 3.64DD260 pKa = 4.91PLPYY264 pKa = 9.87HH265 pKa = 7.05DD266 pKa = 5.95LSDD269 pKa = 4.09SEE271 pKa = 4.4NLSVPDD277 pKa = 5.08AIEE280 pKa = 4.35DD281 pKa = 3.92TSDD284 pKa = 4.18DD285 pKa = 4.09EE286 pKa = 5.9SDD288 pKa = 4.0APPTPSMGRR297 pKa = 11.84STTSLTLLDD306 pKa = 4.34AASIPLPSEE315 pKa = 4.04RR316 pKa = 11.84SSLRR320 pKa = 11.84HH321 pKa = 5.44TNPHH325 pKa = 4.4VHH327 pKa = 5.86VEE329 pKa = 4.06SDD331 pKa = 3.13DD332 pKa = 4.07SYY334 pKa = 11.59FYY336 pKa = 10.6PFEE339 pKa = 5.38LDD341 pKa = 3.43PLPFPEE347 pKa = 5.35DD348 pKa = 2.75HH349 pKa = 6.48HH350 pKa = 7.05TYY352 pKa = 8.76NTYY355 pKa = 9.47QEE357 pKa = 4.49CC358 pKa = 3.69

Molecular weight: 39.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A369JI10|A0A369JI10_HYPMA Uncharacterized protein OS=Hypsizygus marmoreus OX=39966 GN=Hypma_012912 PE=4 SV=1
MM1 pKa = 7.61SSPTRR6 pKa = 11.84HH7 pKa = 5.77PTAASPPSLRR17 pKa = 11.84VTPLTPVTSLLRR29 pKa = 11.84AKK31 pKa = 10.23RR32 pKa = 11.84SAMLSAVPIRR42 pKa = 11.84PAPTTPAPAPTPSSTRR58 pKa = 11.84SLLASTAVLRR68 pKa = 11.84SRR70 pKa = 11.84ARR72 pKa = 11.84AAPWPSPDD80 pKa = 3.65KK81 pKa = 10.98TSLTAA86 pKa = 4.62

Molecular weight: 8.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16572

0

16572

7037948

8

5061

424.7

47.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.186 ± 0.017

1.292 ± 0.007

5.565 ± 0.012

5.711 ± 0.017

3.804 ± 0.01

6.273 ± 0.017

2.778 ± 0.01

4.995 ± 0.014

4.424 ± 0.016

9.335 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.062 ± 0.007

3.422 ± 0.01

6.884 ± 0.023

3.633 ± 0.012

6.342 ± 0.015

8.813 ± 0.024

6.219 ± 0.013

6.192 ± 0.013

1.434 ± 0.007

2.607 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski