Parabacteroides sp. An277

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Tannerellaceae; Parabacteroides; unclassified Parabacteroides

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2949 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4DB68|A0A1Y4DB68_9BACT Uncharacterized protein OS=Parabacteroides sp. An277 OX=1965619 GN=B5F77_02110 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.58KK3 pKa = 10.28NLFYY7 pKa = 10.36TAIFALGMACTFTACSEE24 pKa = 4.03EE25 pKa = 5.13DD26 pKa = 3.75EE27 pKa = 4.97PNPNPSPDD35 pKa = 3.54EE36 pKa = 4.04PTTTSAYY43 pKa = 10.65DD44 pKa = 3.72LDD46 pKa = 4.23YY47 pKa = 10.87TADD50 pKa = 3.8NAAAWGNYY58 pKa = 7.08MYY60 pKa = 9.5QTALLLDD67 pKa = 3.59QDD69 pKa = 3.85ATSLYY74 pKa = 10.23NAWTSDD80 pKa = 3.59YY81 pKa = 10.81EE82 pKa = 4.62GNGPYY87 pKa = 9.29ATIFKK92 pKa = 9.75DD93 pKa = 3.6QTSGAYY99 pKa = 9.58SSPLACIQEE108 pKa = 4.38MVEE111 pKa = 3.83SGMWNIANEE120 pKa = 4.11VGTAKK125 pKa = 10.32IQDD128 pKa = 3.8PYY130 pKa = 8.94NTYY133 pKa = 10.66VSGDD137 pKa = 3.78HH138 pKa = 6.81EE139 pKa = 5.25GGLYY143 pKa = 10.4AVEE146 pKa = 5.26SWFSWHH152 pKa = 7.13SRR154 pKa = 11.84DD155 pKa = 5.48DD156 pKa = 3.47YY157 pKa = 10.93TNNIFSIRR165 pKa = 11.84NTYY168 pKa = 9.12YY169 pKa = 11.15GRR171 pKa = 11.84IDD173 pKa = 4.31DD174 pKa = 4.49NDD176 pKa = 3.24VSKK179 pKa = 10.93IDD181 pKa = 4.23GDD183 pKa = 3.85LSAYY187 pKa = 10.38NSYY190 pKa = 11.57SDD192 pKa = 5.02FDD194 pKa = 4.1DD195 pKa = 5.3AGDD198 pKa = 3.36ISEE201 pKa = 4.74NSLSTFIASINSEE214 pKa = 4.08LDD216 pKa = 3.16EE217 pKa = 4.83TIKK220 pKa = 10.59QQIFAAAKK228 pKa = 9.29AIQAIPQPFRR238 pKa = 11.84NNIDD242 pKa = 3.51SEE244 pKa = 4.5EE245 pKa = 4.15SVAAMDD251 pKa = 4.24ACNTLASTLLNEE263 pKa = 5.01LLPFVNTLSGAEE275 pKa = 3.96YY276 pKa = 10.23TDD278 pKa = 3.59RR279 pKa = 11.84LDD281 pKa = 5.39AIAEE285 pKa = 4.06QFVDD289 pKa = 4.36VVVLPTYY296 pKa = 10.74QDD298 pKa = 3.41LQEE301 pKa = 4.46KK302 pKa = 10.18NRR304 pKa = 11.84TLLDD308 pKa = 3.26VVNQFRR314 pKa = 11.84EE315 pKa = 4.27NPSDD319 pKa = 3.73ANFEE323 pKa = 4.41NVCNAWLEE331 pKa = 4.02ARR333 pKa = 11.84EE334 pKa = 3.96PWEE337 pKa = 3.86KK338 pKa = 11.12SEE340 pKa = 4.51AFLIGPVANWGLDD353 pKa = 3.47PNMDD357 pKa = 3.49SWPLDD362 pKa = 3.3VNAIVQLLEE371 pKa = 4.04SQNWNEE377 pKa = 4.26MQWSGDD383 pKa = 3.61YY384 pKa = 11.36NEE386 pKa = 5.1DD387 pKa = 3.76SEE389 pKa = 5.44TIGAAQNVRR398 pKa = 11.84GYY400 pKa = 8.55HH401 pKa = 4.36TLEE404 pKa = 3.82FLSFKK409 pKa = 10.58NGEE412 pKa = 3.92PRR414 pKa = 11.84KK415 pKa = 10.31VNNN418 pKa = 3.81

Molecular weight: 46.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4CY53|A0A1Y4CY53_9BACT Phenylacetic acid degradation protein OS=Parabacteroides sp. An277 OX=1965619 GN=B5F77_09460 PE=4 SV=1
MM1 pKa = 7.62FEE3 pKa = 4.73LLLIIYY9 pKa = 7.15LVPVIIILSLLWRR22 pKa = 11.84LVLWLVRR29 pKa = 11.84NVLRR33 pKa = 11.84LAGWLLKK40 pKa = 10.2KK41 pKa = 10.62VCVLVWKK48 pKa = 10.52GLLLLVGIFLGRR60 pKa = 11.84CCVNRR65 pKa = 11.84PPDD68 pKa = 3.45SRR70 pKa = 4.23

Molecular weight: 8.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2949

0

2949

1065573

28

2472

361.3

40.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.71 ± 0.044

1.21 ± 0.019

5.484 ± 0.029

6.963 ± 0.043

4.436 ± 0.027

6.925 ± 0.043

2.012 ± 0.024

6.412 ± 0.037

5.845 ± 0.042

9.213 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.649 ± 0.018

4.645 ± 0.044

3.943 ± 0.027

3.749 ± 0.026

5.036 ± 0.04

5.759 ± 0.032

5.675 ± 0.039

6.581 ± 0.039

1.345 ± 0.02

4.41 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski