Amino acid dipepetide frequency for Mycobacterium phage Ovechkin

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.112AlaAla: 15.112 ± 1.867
0.857AlaCys: 0.857 ± 0.222
7.717AlaAsp: 7.717 ± 0.683
7.931AlaGlu: 7.931 ± 0.794
2.465AlaPhe: 2.465 ± 0.372
10.074AlaGly: 10.074 ± 1.131
2.197AlaHis: 2.197 ± 0.361
3.912AlaIle: 3.912 ± 0.461
3.965AlaLys: 3.965 ± 0.457
8.788AlaLeu: 8.788 ± 0.767
2.626AlaMet: 2.626 ± 0.394
2.197AlaAsn: 2.197 ± 0.36
4.876AlaPro: 4.876 ± 0.547
3.537AlaGln: 3.537 ± 0.427
7.342AlaArg: 7.342 ± 0.75
5.466AlaSer: 5.466 ± 0.602
6.377AlaThr: 6.377 ± 0.6
6.698AlaVal: 6.698 ± 0.622
2.787AlaTrp: 2.787 ± 0.509
2.251AlaTyr: 2.251 ± 0.334
0.0AlaXaa: 0.0 ± 0.0
Cys
1.072CysAla: 1.072 ± 0.361
0.161CysCys: 0.161 ± 0.092
1.233CysAsp: 1.233 ± 0.31
0.589CysGlu: 0.589 ± 0.172
0.375CysPhe: 0.375 ± 0.172
1.447CysGly: 1.447 ± 0.371
0.268CysHis: 0.268 ± 0.128
0.054CysIle: 0.054 ± 0.056
0.375CysLys: 0.375 ± 0.129
0.965CysLeu: 0.965 ± 0.286
0.214CysMet: 0.214 ± 0.123
0.536CysAsn: 0.536 ± 0.179
1.179CysPro: 1.179 ± 0.283
0.322CysGln: 0.322 ± 0.112
0.965CysArg: 0.965 ± 0.298
0.965CysSer: 0.965 ± 0.255
0.75CysThr: 0.75 ± 0.222
0.697CysVal: 0.697 ± 0.199
0.268CysTrp: 0.268 ± 0.112
0.107CysTyr: 0.107 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
6.806AspAla: 6.806 ± 0.574
0.75AspCys: 0.75 ± 0.168
4.716AspAsp: 4.716 ± 0.515
3.162AspGlu: 3.162 ± 0.427
1.929AspPhe: 1.929 ± 0.294
7.181AspGly: 7.181 ± 0.733
1.447AspHis: 1.447 ± 0.238
2.679AspIle: 2.679 ± 0.382
1.876AspLys: 1.876 ± 0.362
5.895AspLeu: 5.895 ± 0.696
0.911AspMet: 0.911 ± 0.23
1.715AspAsn: 1.715 ± 0.336
4.769AspPro: 4.769 ± 0.566
2.304AspGln: 2.304 ± 0.322
5.305AspArg: 5.305 ± 0.608
3.001AspSer: 3.001 ± 0.432
3.912AspThr: 3.912 ± 0.498
4.448AspVal: 4.448 ± 0.626
1.286AspTrp: 1.286 ± 0.277
1.822AspTyr: 1.822 ± 0.325
0.0AspXaa: 0.0 ± 0.0
Glu
5.573GluAla: 5.573 ± 0.601
0.857GluCys: 0.857 ± 0.23
3.001GluAsp: 3.001 ± 0.374
2.84GluGlu: 2.84 ± 0.558
2.197GluPhe: 2.197 ± 0.313
3.59GluGly: 3.59 ± 0.511
1.233GluHis: 1.233 ± 0.321
2.197GluIle: 2.197 ± 0.353
1.822GluLys: 1.822 ± 0.314
4.876GluLeu: 4.876 ± 0.595
1.554GluMet: 1.554 ± 0.315
2.144GluAsn: 2.144 ± 0.281
2.733GluPro: 2.733 ± 0.447
2.947GluGln: 2.947 ± 0.429
4.93GluArg: 4.93 ± 0.608
2.84GluSer: 2.84 ± 0.427
3.858GluThr: 3.858 ± 0.647
3.912GluVal: 3.912 ± 0.445
1.983GluTrp: 1.983 ± 0.304
1.876GluTyr: 1.876 ± 0.305
0.0GluXaa: 0.0 ± 0.0
Phe
2.787PheAla: 2.787 ± 0.368
0.375PheCys: 0.375 ± 0.139
2.197PheAsp: 2.197 ± 0.432
1.5PheGlu: 1.5 ± 0.306
0.911PhePhe: 0.911 ± 0.282
3.108PheGly: 3.108 ± 0.606
0.429PheHis: 0.429 ± 0.126
1.393PheIle: 1.393 ± 0.288
1.072PheLys: 1.072 ± 0.222
1.715PheLeu: 1.715 ± 0.258
0.857PheMet: 0.857 ± 0.277
1.34PheAsn: 1.34 ± 0.339
1.661PhePro: 1.661 ± 0.345
1.072PheGln: 1.072 ± 0.308
1.608PheArg: 1.608 ± 0.283
1.5PheSer: 1.5 ± 0.257
2.144PheThr: 2.144 ± 0.406
2.144PheVal: 2.144 ± 0.256
0.589PheTrp: 0.589 ± 0.141
1.018PheTyr: 1.018 ± 0.272
0.0PheXaa: 0.0 ± 0.0
Gly
9.003GlyAla: 9.003 ± 1.152
1.072GlyCys: 1.072 ± 0.319
6.645GlyAsp: 6.645 ± 0.718
3.858GlyGlu: 3.858 ± 0.547
2.787GlyPhe: 2.787 ± 0.463
11.039GlyGly: 11.039 ± 2.286
1.715GlyHis: 1.715 ± 0.321
4.341GlyIle: 4.341 ± 0.61
2.626GlyLys: 2.626 ± 0.466
6.055GlyLeu: 6.055 ± 0.49
2.304GlyMet: 2.304 ± 0.425
3.322GlyAsn: 3.322 ± 0.47
4.555GlyPro: 4.555 ± 0.503
2.358GlyGln: 2.358 ± 0.52
5.412GlyArg: 5.412 ± 0.706
5.198GlySer: 5.198 ± 0.852
6.591GlyThr: 6.591 ± 0.825
5.787GlyVal: 5.787 ± 0.62
2.947GlyTrp: 2.947 ± 0.383
2.197GlyTyr: 2.197 ± 0.363
0.0GlyXaa: 0.0 ± 0.0
His
1.661HisAla: 1.661 ± 0.293
0.482HisCys: 0.482 ± 0.185
1.018HisAsp: 1.018 ± 0.231
1.34HisGlu: 1.34 ± 0.251
0.589HisPhe: 0.589 ± 0.162
2.144HisGly: 2.144 ± 0.34
0.911HisHis: 0.911 ± 0.242
1.233HisIle: 1.233 ± 0.302
0.911HisLys: 0.911 ± 0.23
1.34HisLeu: 1.34 ± 0.239
0.697HisMet: 0.697 ± 0.221
0.75HisAsn: 0.75 ± 0.176
1.715HisPro: 1.715 ± 0.272
1.179HisGln: 1.179 ± 0.267
2.358HisArg: 2.358 ± 0.387
0.697HisSer: 0.697 ± 0.17
1.34HisThr: 1.34 ± 0.295
1.233HisVal: 1.233 ± 0.263
0.482HisTrp: 0.482 ± 0.151
0.804HisTyr: 0.804 ± 0.21
0.0HisXaa: 0.0 ± 0.0
Ile
5.841IleAla: 5.841 ± 0.62
0.589IleCys: 0.589 ± 0.191
3.537IleAsp: 3.537 ± 0.421
3.162IleGlu: 3.162 ± 0.342
0.911IlePhe: 0.911 ± 0.269
3.59IleGly: 3.59 ± 0.514
1.393IleHis: 1.393 ± 0.286
1.286IleIle: 1.286 ± 0.248
1.072IleLys: 1.072 ± 0.245
2.144IleLeu: 2.144 ± 0.419
0.375IleMet: 0.375 ± 0.125
2.144IleAsn: 2.144 ± 0.316
2.787IlePro: 2.787 ± 0.334
1.34IleGln: 1.34 ± 0.302
2.572IleArg: 2.572 ± 0.422
2.197IleSer: 2.197 ± 0.377
3.322IleThr: 3.322 ± 0.442
2.572IleVal: 2.572 ± 0.391
0.857IleTrp: 0.857 ± 0.222
0.75IleTyr: 0.75 ± 0.201
0.0IleXaa: 0.0 ± 0.0
Lys
3.43LysAla: 3.43 ± 0.502
0.375LysCys: 0.375 ± 0.142
1.5LysAsp: 1.5 ± 0.287
1.447LysGlu: 1.447 ± 0.266
1.233LysPhe: 1.233 ± 0.223
2.679LysGly: 2.679 ± 0.354
1.125LysHis: 1.125 ± 0.263
1.179LysIle: 1.179 ± 0.253
1.34LysLys: 1.34 ± 0.358
2.358LysLeu: 2.358 ± 0.406
0.697LysMet: 0.697 ± 0.177
0.965LysAsn: 0.965 ± 0.222
3.001LysPro: 3.001 ± 0.456
1.5LysGln: 1.5 ± 0.205
2.519LysArg: 2.519 ± 0.462
1.876LysSer: 1.876 ± 0.26
1.768LysThr: 1.768 ± 0.31
2.679LysVal: 2.679 ± 0.399
0.589LysTrp: 0.589 ± 0.201
1.018LysTyr: 1.018 ± 0.283
0.0LysXaa: 0.0 ± 0.0
Leu
7.824LeuAla: 7.824 ± 0.677
0.804LeuCys: 0.804 ± 0.234
4.823LeuAsp: 4.823 ± 0.548
3.43LeuGlu: 3.43 ± 0.411
2.626LeuPhe: 2.626 ± 0.344
5.305LeuGly: 5.305 ± 0.6
1.125LeuHis: 1.125 ± 0.287
3.108LeuIle: 3.108 ± 0.432
2.036LeuLys: 2.036 ± 0.298
4.876LeuLeu: 4.876 ± 0.691
1.233LeuMet: 1.233 ± 0.285
2.572LeuAsn: 2.572 ± 0.388
5.948LeuPro: 5.948 ± 0.729
2.519LeuGln: 2.519 ± 0.379
5.412LeuArg: 5.412 ± 0.697
5.305LeuSer: 5.305 ± 0.581
5.841LeuThr: 5.841 ± 0.522
5.091LeuVal: 5.091 ± 0.519
1.447LeuTrp: 1.447 ± 0.323
1.768LeuTyr: 1.768 ± 0.348
0.0LeuXaa: 0.0 ± 0.0
Met
2.251MetAla: 2.251 ± 0.357
0.161MetCys: 0.161 ± 0.104
1.393MetAsp: 1.393 ± 0.294
0.911MetGlu: 0.911 ± 0.205
0.75MetPhe: 0.75 ± 0.213
1.447MetGly: 1.447 ± 0.298
0.375MetHis: 0.375 ± 0.135
0.643MetIle: 0.643 ± 0.187
0.75MetLys: 0.75 ± 0.207
1.876MetLeu: 1.876 ± 0.269
0.643MetMet: 0.643 ± 0.237
0.911MetAsn: 0.911 ± 0.19
1.125MetPro: 1.125 ± 0.223
0.322MetGln: 0.322 ± 0.12
1.447MetArg: 1.447 ± 0.265
2.84MetSer: 2.84 ± 0.399
2.144MetThr: 2.144 ± 0.376
1.34MetVal: 1.34 ± 0.37
0.322MetTrp: 0.322 ± 0.126
0.589MetTyr: 0.589 ± 0.208
0.0MetXaa: 0.0 ± 0.0
Asn
3.965AsnAla: 3.965 ± 0.393
0.161AsnCys: 0.161 ± 0.083
2.036AsnAsp: 2.036 ± 0.341
1.715AsnGlu: 1.715 ± 0.317
0.965AsnPhe: 0.965 ± 0.282
4.073AsnGly: 4.073 ± 0.535
0.965AsnHis: 0.965 ± 0.204
1.393AsnIle: 1.393 ± 0.394
1.125AsnLys: 1.125 ± 0.258
2.465AsnLeu: 2.465 ± 0.399
0.482AsnMet: 0.482 ± 0.17
1.554AsnAsn: 1.554 ± 0.324
3.001AsnPro: 3.001 ± 0.483
1.018AsnGln: 1.018 ± 0.304
2.304AsnArg: 2.304 ± 0.345
1.715AsnSer: 1.715 ± 0.328
2.251AsnThr: 2.251 ± 0.297
1.715AsnVal: 1.715 ± 0.336
0.643AsnTrp: 0.643 ± 0.15
0.589AsnTyr: 0.589 ± 0.17
0.0AsnXaa: 0.0 ± 0.0
Pro
5.895ProAla: 5.895 ± 0.602
0.804ProCys: 0.804 ± 0.266
4.126ProAsp: 4.126 ± 0.508
4.019ProGlu: 4.019 ± 0.533
1.822ProPhe: 1.822 ± 0.366
6.591ProGly: 6.591 ± 0.73
1.5ProHis: 1.5 ± 0.314
2.304ProIle: 2.304 ± 0.272
2.197ProLys: 2.197 ± 0.393
4.394ProLeu: 4.394 ± 0.579
1.34ProMet: 1.34 ± 0.315
2.626ProAsn: 2.626 ± 0.394
4.233ProPro: 4.233 ± 0.638
2.304ProGln: 2.304 ± 0.36
3.43ProArg: 3.43 ± 0.621
3.322ProSer: 3.322 ± 0.46
3.59ProThr: 3.59 ± 0.567
4.876ProVal: 4.876 ± 0.604
1.018ProTrp: 1.018 ± 0.218
1.5ProTyr: 1.5 ± 0.304
0.0ProXaa: 0.0 ± 0.0
Gln
4.501GlnAla: 4.501 ± 0.579
0.536GlnCys: 0.536 ± 0.184
1.5GlnAsp: 1.5 ± 0.228
1.554GlnGlu: 1.554 ± 0.261
1.125GlnPhe: 1.125 ± 0.228
2.197GlnGly: 2.197 ± 0.451
0.804GlnHis: 0.804 ± 0.22
1.608GlnIle: 1.608 ± 0.31
1.018GlnLys: 1.018 ± 0.194
3.376GlnLeu: 3.376 ± 0.425
0.536GlnMet: 0.536 ± 0.159
1.018GlnAsn: 1.018 ± 0.262
2.304GlnPro: 2.304 ± 0.389
1.072GlnGln: 1.072 ± 0.272
2.572GlnArg: 2.572 ± 0.379
2.251GlnSer: 2.251 ± 0.347
1.929GlnThr: 1.929 ± 0.352
2.411GlnVal: 2.411 ± 0.365
0.75GlnTrp: 0.75 ± 0.206
0.697GlnTyr: 0.697 ± 0.261
0.0GlnXaa: 0.0 ± 0.0
Arg
7.127ArgAla: 7.127 ± 0.768
1.286ArgCys: 1.286 ± 0.323
3.912ArgAsp: 3.912 ± 0.524
5.305ArgGlu: 5.305 ± 0.715
1.983ArgPhe: 1.983 ± 0.324
4.448ArgGly: 4.448 ± 0.477
1.876ArgHis: 1.876 ± 0.39
3.858ArgIle: 3.858 ± 0.473
1.983ArgLys: 1.983 ± 0.377
5.037ArgLeu: 5.037 ± 0.67
2.626ArgMet: 2.626 ± 0.405
2.411ArgAsn: 2.411 ± 0.391
3.43ArgPro: 3.43 ± 0.406
2.09ArgGln: 2.09 ± 0.397
5.948ArgArg: 5.948 ± 0.788
3.751ArgSer: 3.751 ± 0.358
3.965ArgThr: 3.965 ± 0.556
5.52ArgVal: 5.52 ± 0.587
1.983ArgTrp: 1.983 ± 0.372
2.144ArgTyr: 2.144 ± 0.359
0.0ArgXaa: 0.0 ± 0.0
Ser
6.323SerAla: 6.323 ± 1.056
0.643SerCys: 0.643 ± 0.23
4.341SerAsp: 4.341 ± 0.429
2.894SerGlu: 2.894 ± 0.419
1.768SerPhe: 1.768 ± 0.436
6.377SerGly: 6.377 ± 0.735
1.286SerHis: 1.286 ± 0.254
3.001SerIle: 3.001 ± 0.45
2.733SerLys: 2.733 ± 0.446
3.751SerLeu: 3.751 ± 0.499
1.125SerMet: 1.125 ± 0.233
1.929SerAsn: 1.929 ± 0.366
3.215SerPro: 3.215 ± 0.343
1.661SerGln: 1.661 ± 0.295
3.751SerArg: 3.751 ± 0.51
3.537SerSer: 3.537 ± 0.578
2.947SerThr: 2.947 ± 0.385
3.751SerVal: 3.751 ± 0.407
1.715SerTrp: 1.715 ± 0.276
1.393SerTyr: 1.393 ± 0.209
0.0SerXaa: 0.0 ± 0.0
Thr
6.377ThrAla: 6.377 ± 0.578
0.857ThrCys: 0.857 ± 0.233
3.751ThrAsp: 3.751 ± 0.568
3.483ThrGlu: 3.483 ± 0.376
1.768ThrPhe: 1.768 ± 0.292
5.466ThrGly: 5.466 ± 0.581
1.554ThrHis: 1.554 ± 0.268
3.483ThrIle: 3.483 ± 0.412
2.304ThrLys: 2.304 ± 0.385
4.501ThrLeu: 4.501 ± 0.526
1.393ThrMet: 1.393 ± 0.245
2.572ThrAsn: 2.572 ± 0.352
5.091ThrPro: 5.091 ± 0.666
2.09ThrGln: 2.09 ± 0.342
4.18ThrArg: 4.18 ± 0.454
3.751ThrSer: 3.751 ± 0.449
4.609ThrThr: 4.609 ± 0.646
5.948ThrVal: 5.948 ± 0.692
0.965ThrTrp: 0.965 ± 0.265
1.983ThrTyr: 1.983 ± 0.268
0.0ThrXaa: 0.0 ± 0.0
Val
6.859ValAla: 6.859 ± 0.62
1.125ValCys: 1.125 ± 0.267
5.144ValAsp: 5.144 ± 0.499
4.769ValGlu: 4.769 ± 0.591
2.036ValPhe: 2.036 ± 0.388
6.055ValGly: 6.055 ± 0.75
1.447ValHis: 1.447 ± 0.264
2.626ValIle: 2.626 ± 0.415
2.251ValLys: 2.251 ± 0.37
5.144ValLeu: 5.144 ± 0.551
1.5ValMet: 1.5 ± 0.219
2.197ValAsn: 2.197 ± 0.346
3.805ValPro: 3.805 ± 0.47
2.626ValGln: 2.626 ± 0.301
4.287ValArg: 4.287 ± 0.565
5.359ValSer: 5.359 ± 0.557
4.984ValThr: 4.984 ± 0.489
5.895ValVal: 5.895 ± 0.707
1.5ValTrp: 1.5 ± 0.247
1.393ValTyr: 1.393 ± 0.253
0.0ValXaa: 0.0 ± 0.0
Trp
2.09TrpAla: 2.09 ± 0.323
0.268TrpCys: 0.268 ± 0.116
1.661TrpAsp: 1.661 ± 0.335
1.233TrpGlu: 1.233 ± 0.3
0.536TrpPhe: 0.536 ± 0.162
1.072TrpGly: 1.072 ± 0.237
0.75TrpHis: 0.75 ± 0.204
1.179TrpIle: 1.179 ± 0.224
1.125TrpLys: 1.125 ± 0.231
1.715TrpLeu: 1.715 ± 0.374
0.857TrpMet: 0.857 ± 0.275
0.589TrpAsn: 0.589 ± 0.183
0.911TrpPro: 0.911 ± 0.263
0.75TrpGln: 0.75 ± 0.226
2.197TrpArg: 2.197 ± 0.401
1.661TrpSer: 1.661 ± 0.423
1.768TrpThr: 1.768 ± 0.263
1.983TrpVal: 1.983 ± 0.448
0.857TrpTrp: 0.857 ± 0.172
0.375TrpTyr: 0.375 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.679TyrAla: 2.679 ± 0.434
0.375TyrCys: 0.375 ± 0.159
1.661TyrAsp: 1.661 ± 0.36
1.822TyrGlu: 1.822 ± 0.331
0.643TyrPhe: 0.643 ± 0.196
1.929TyrGly: 1.929 ± 0.377
0.482TyrHis: 0.482 ± 0.147
0.965TyrIle: 0.965 ± 0.235
0.697TyrLys: 0.697 ± 0.214
1.822TyrLeu: 1.822 ± 0.265
0.214TyrMet: 0.214 ± 0.105
0.643TyrAsn: 0.643 ± 0.176
1.608TyrPro: 1.608 ± 0.266
0.75TyrGln: 0.75 ± 0.206
2.144TyrArg: 2.144 ± 0.347
0.911TyrSer: 0.911 ± 0.182
2.036TyrThr: 2.036 ± 0.38
2.304TyrVal: 2.304 ± 0.338
0.589TyrTrp: 0.589 ± 0.167
0.643TyrTyr: 0.643 ± 0.158
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 106 proteins (18662 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski