Amino acid dipepetide frequency for Mycobacterium phage DarthPhader

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.331AlaAla: 11.331 ± 0.943
0.596AlaCys: 0.596 ± 0.19
5.844AlaAsp: 5.844 ± 0.58
7.455AlaGlu: 7.455 ± 0.825
3.817AlaPhe: 3.817 ± 0.433
9.482AlaGly: 9.482 ± 1.171
1.61AlaHis: 1.61 ± 0.301
4.354AlaIle: 4.354 ± 0.442
5.129AlaLys: 5.129 ± 0.602
8.647AlaLeu: 8.647 ± 1.058
3.042AlaMet: 3.042 ± 0.437
2.743AlaAsn: 2.743 ± 0.373
5.248AlaPro: 5.248 ± 0.597
3.996AlaGln: 3.996 ± 0.428
6.262AlaArg: 6.262 ± 0.622
6.381AlaSer: 6.381 ± 0.531
4.771AlaThr: 4.771 ± 0.658
7.753AlaVal: 7.753 ± 0.812
1.61AlaTrp: 1.61 ± 0.282
2.147AlaTyr: 2.147 ± 0.411
0.0AlaXaa: 0.0 ± 0.0
Cys
0.954CysAla: 0.954 ± 0.182
0.06CysCys: 0.06 ± 0.051
0.477CysAsp: 0.477 ± 0.152
0.358CysGlu: 0.358 ± 0.146
0.417CysPhe: 0.417 ± 0.145
0.835CysGly: 0.835 ± 0.211
0.239CysHis: 0.239 ± 0.149
0.179CysIle: 0.179 ± 0.127
0.358CysLys: 0.358 ± 0.143
0.716CysLeu: 0.716 ± 0.187
0.119CysMet: 0.119 ± 0.085
0.417CysAsn: 0.417 ± 0.155
0.358CysPro: 0.358 ± 0.144
0.119CysGln: 0.119 ± 0.084
0.537CysArg: 0.537 ± 0.218
0.358CysSer: 0.358 ± 0.167
0.596CysThr: 0.596 ± 0.182
0.537CysVal: 0.537 ± 0.167
0.358CysTrp: 0.358 ± 0.134
0.298CysTyr: 0.298 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
5.188AspAla: 5.188 ± 0.49
0.477AspCys: 0.477 ± 0.188
3.638AspAsp: 3.638 ± 0.474
4.175AspGlu: 4.175 ± 0.586
2.385AspPhe: 2.385 ± 0.419
4.831AspGly: 4.831 ± 0.572
1.431AspHis: 1.431 ± 0.335
3.698AspIle: 3.698 ± 0.479
2.863AspLys: 2.863 ± 0.346
5.844AspLeu: 5.844 ± 0.776
1.312AspMet: 1.312 ± 0.281
1.372AspAsn: 1.372 ± 0.281
4.055AspPro: 4.055 ± 0.593
2.147AspGln: 2.147 ± 0.412
2.982AspArg: 2.982 ± 0.462
3.876AspSer: 3.876 ± 0.487
3.34AspThr: 3.34 ± 0.363
4.055AspVal: 4.055 ± 0.544
0.835AspTrp: 0.835 ± 0.195
2.087AspTyr: 2.087 ± 0.359
0.0AspXaa: 0.0 ± 0.0
Glu
7.455GluAla: 7.455 ± 0.749
0.179GluCys: 0.179 ± 0.086
3.28GluAsp: 3.28 ± 0.607
3.876GluGlu: 3.876 ± 0.515
2.684GluPhe: 2.684 ± 0.406
5.248GluGly: 5.248 ± 0.55
1.431GluHis: 1.431 ± 0.34
3.757GluIle: 3.757 ± 0.41
2.982GluLys: 2.982 ± 0.443
5.546GluLeu: 5.546 ± 0.551
2.028GluMet: 2.028 ± 0.34
1.968GluAsn: 1.968 ± 0.316
2.028GluPro: 2.028 ± 0.317
2.207GluGln: 2.207 ± 0.332
4.89GluArg: 4.89 ± 0.649
3.34GluSer: 3.34 ± 0.445
3.876GluThr: 3.876 ± 0.445
4.055GluVal: 4.055 ± 0.478
1.312GluTrp: 1.312 ± 0.249
1.67GluTyr: 1.67 ± 0.338
0.0GluXaa: 0.0 ± 0.0
Phe
3.817PheAla: 3.817 ± 0.499
0.417PheCys: 0.417 ± 0.147
1.968PheAsp: 1.968 ± 0.403
2.266PheGlu: 2.266 ± 0.421
0.895PhePhe: 0.895 ± 0.199
3.161PheGly: 3.161 ± 0.464
0.775PheHis: 0.775 ± 0.227
1.849PheIle: 1.849 ± 0.294
1.133PheLys: 1.133 ± 0.231
2.445PheLeu: 2.445 ± 0.409
0.239PheMet: 0.239 ± 0.132
1.908PheAsn: 1.908 ± 0.302
1.849PhePro: 1.849 ± 0.358
0.954PheGln: 0.954 ± 0.263
1.789PheArg: 1.789 ± 0.27
2.087PheSer: 2.087 ± 0.274
2.505PheThr: 2.505 ± 0.338
2.207PheVal: 2.207 ± 0.419
0.477PheTrp: 0.477 ± 0.191
0.716PheTyr: 0.716 ± 0.194
0.0PheXaa: 0.0 ± 0.0
Gly
7.812GlyAla: 7.812 ± 0.967
0.775GlyCys: 0.775 ± 0.216
6.322GlyAsp: 6.322 ± 0.736
4.771GlyGlu: 4.771 ± 0.561
2.445GlyPhe: 2.445 ± 0.35
11.808GlyGly: 11.808 ± 3.132
1.551GlyHis: 1.551 ± 0.344
4.89GlyIle: 4.89 ± 0.733
4.473GlyLys: 4.473 ± 0.513
5.546GlyLeu: 5.546 ± 0.621
2.505GlyMet: 2.505 ± 0.34
3.459GlyAsn: 3.459 ± 0.515
4.175GlyPro: 4.175 ± 1.06
2.743GlyGln: 2.743 ± 0.37
4.711GlyArg: 4.711 ± 0.463
5.248GlySer: 5.248 ± 1.05
5.725GlyThr: 5.725 ± 0.567
6.083GlyVal: 6.083 ± 0.662
2.385GlyTrp: 2.385 ± 0.343
2.743GlyTyr: 2.743 ± 0.398
0.0GlyXaa: 0.0 ± 0.0
His
1.312HisAla: 1.312 ± 0.315
0.119HisCys: 0.119 ± 0.09
1.073HisAsp: 1.073 ± 0.246
1.073HisGlu: 1.073 ± 0.313
0.835HisPhe: 0.835 ± 0.247
1.491HisGly: 1.491 ± 0.383
0.537HisHis: 0.537 ± 0.192
0.775HisIle: 0.775 ± 0.246
0.537HisLys: 0.537 ± 0.177
1.431HisLeu: 1.431 ± 0.278
0.537HisMet: 0.537 ± 0.2
0.477HisAsn: 0.477 ± 0.136
1.312HisPro: 1.312 ± 0.248
0.716HisGln: 0.716 ± 0.193
1.789HisArg: 1.789 ± 0.312
1.014HisSer: 1.014 ± 0.254
1.073HisThr: 1.073 ± 0.226
1.729HisVal: 1.729 ± 0.32
0.298HisTrp: 0.298 ± 0.15
0.537HisTyr: 0.537 ± 0.25
0.0HisXaa: 0.0 ± 0.0
Ile
4.95IleAla: 4.95 ± 0.441
0.417IleCys: 0.417 ± 0.134
4.592IleAsp: 4.592 ± 0.471
5.069IleGlu: 5.069 ± 0.534
1.193IlePhe: 1.193 ± 0.245
3.28IleGly: 3.28 ± 0.419
0.775IleHis: 0.775 ± 0.206
2.028IleIle: 2.028 ± 0.372
2.863IleLys: 2.863 ± 0.408
3.578IleLeu: 3.578 ± 0.377
0.835IleMet: 0.835 ± 0.239
2.028IleAsn: 2.028 ± 0.322
4.473IlePro: 4.473 ± 0.428
1.729IleGln: 1.729 ± 0.428
3.101IleArg: 3.101 ± 0.34
2.803IleSer: 2.803 ± 0.478
2.803IleThr: 2.803 ± 0.35
3.459IleVal: 3.459 ± 0.457
0.537IleTrp: 0.537 ± 0.154
0.954IleTyr: 0.954 ± 0.23
0.0IleXaa: 0.0 ± 0.0
Lys
5.188LysAla: 5.188 ± 0.548
0.179LysCys: 0.179 ± 0.091
2.207LysAsp: 2.207 ± 0.289
2.564LysGlu: 2.564 ± 0.377
1.193LysPhe: 1.193 ± 0.213
3.817LysGly: 3.817 ± 0.537
0.596LysHis: 0.596 ± 0.19
1.67LysIle: 1.67 ± 0.333
2.922LysLys: 2.922 ± 0.485
4.354LysLeu: 4.354 ± 0.496
1.014LysMet: 1.014 ± 0.341
1.491LysAsn: 1.491 ± 0.297
2.326LysPro: 2.326 ± 0.402
1.133LysGln: 1.133 ± 0.237
3.042LysArg: 3.042 ± 0.534
3.161LysSer: 3.161 ± 0.415
2.803LysThr: 2.803 ± 0.379
3.876LysVal: 3.876 ± 0.48
0.596LysTrp: 0.596 ± 0.186
1.67LysTyr: 1.67 ± 0.396
0.0LysXaa: 0.0 ± 0.0
Leu
8.886LeuAla: 8.886 ± 0.859
0.775LeuCys: 0.775 ± 0.19
4.652LeuAsp: 4.652 ± 0.591
4.115LeuGlu: 4.115 ± 0.5
2.326LeuPhe: 2.326 ± 0.295
6.381LeuGly: 6.381 ± 0.781
1.968LeuHis: 1.968 ± 0.389
4.592LeuIle: 4.592 ± 0.443
3.698LeuLys: 3.698 ± 0.494
5.606LeuLeu: 5.606 ± 0.645
2.087LeuMet: 2.087 ± 0.368
2.266LeuAsn: 2.266 ± 0.356
4.532LeuPro: 4.532 ± 0.534
2.207LeuGln: 2.207 ± 0.466
5.785LeuArg: 5.785 ± 0.706
6.023LeuSer: 6.023 ± 0.628
5.248LeuThr: 5.248 ± 0.52
4.711LeuVal: 4.711 ± 0.589
1.551LeuTrp: 1.551 ± 0.278
2.087LeuTyr: 2.087 ± 0.425
0.0LeuXaa: 0.0 ± 0.0
Met
3.101MetAla: 3.101 ± 0.356
0.06MetCys: 0.06 ± 0.056
1.133MetAsp: 1.133 ± 0.255
1.252MetGlu: 1.252 ± 0.234
0.954MetPhe: 0.954 ± 0.226
1.849MetGly: 1.849 ± 0.38
0.358MetHis: 0.358 ± 0.139
1.193MetIle: 1.193 ± 0.316
1.61MetLys: 1.61 ± 0.27
1.372MetLeu: 1.372 ± 0.321
0.537MetMet: 0.537 ± 0.167
0.895MetAsn: 0.895 ± 0.186
1.491MetPro: 1.491 ± 0.337
1.073MetGln: 1.073 ± 0.22
1.252MetArg: 1.252 ± 0.249
2.385MetSer: 2.385 ± 0.423
1.908MetThr: 1.908 ± 0.267
1.61MetVal: 1.61 ± 0.327
0.417MetTrp: 0.417 ± 0.13
0.656MetTyr: 0.656 ± 0.197
0.0MetXaa: 0.0 ± 0.0
Asn
3.101AsnAla: 3.101 ± 0.406
0.298AsnCys: 0.298 ± 0.131
1.312AsnAsp: 1.312 ± 0.233
1.908AsnGlu: 1.908 ± 0.363
0.716AsnPhe: 0.716 ± 0.243
3.757AsnGly: 3.757 ± 0.481
0.716AsnHis: 0.716 ± 0.175
1.491AsnIle: 1.491 ± 0.268
1.073AsnLys: 1.073 ± 0.25
3.459AsnLeu: 3.459 ± 0.461
0.954AsnMet: 0.954 ± 0.261
1.073AsnAsn: 1.073 ± 0.268
2.743AsnPro: 2.743 ± 0.363
1.193AsnGln: 1.193 ± 0.289
1.61AsnArg: 1.61 ± 0.286
1.61AsnSer: 1.61 ± 0.301
1.372AsnThr: 1.372 ± 0.257
2.445AsnVal: 2.445 ± 0.327
0.477AsnTrp: 0.477 ± 0.168
0.656AsnTyr: 0.656 ± 0.169
0.0AsnXaa: 0.0 ± 0.0
Pro
5.487ProAla: 5.487 ± 0.688
0.239ProCys: 0.239 ± 0.129
3.698ProAsp: 3.698 ± 0.434
4.592ProGlu: 4.592 ± 0.518
1.729ProPhe: 1.729 ± 0.341
4.831ProGly: 4.831 ± 0.626
1.133ProHis: 1.133 ± 0.224
2.803ProIle: 2.803 ± 0.403
1.968ProLys: 1.968 ± 0.391
3.28ProLeu: 3.28 ± 0.436
1.372ProMet: 1.372 ± 0.282
1.908ProAsn: 1.908 ± 0.348
2.028ProPro: 2.028 ± 0.369
2.505ProGln: 2.505 ± 0.521
3.22ProArg: 3.22 ± 0.524
2.624ProSer: 2.624 ± 0.441
3.936ProThr: 3.936 ± 0.459
3.578ProVal: 3.578 ± 0.504
0.895ProTrp: 0.895 ± 0.267
1.551ProTyr: 1.551 ± 0.302
0.0ProXaa: 0.0 ± 0.0
Gln
4.95GlnAla: 4.95 ± 0.53
0.298GlnCys: 0.298 ± 0.135
1.61GlnAsp: 1.61 ± 0.34
1.431GlnGlu: 1.431 ± 0.24
1.252GlnPhe: 1.252 ± 0.238
3.757GlnGly: 3.757 ± 1.398
0.537GlnHis: 0.537 ± 0.191
2.564GlnIle: 2.564 ± 0.428
1.431GlnLys: 1.431 ± 0.262
3.34GlnLeu: 3.34 ± 0.712
0.954GlnMet: 0.954 ± 0.235
0.596GlnAsn: 0.596 ± 0.184
1.491GlnPro: 1.491 ± 0.302
2.385GlnGln: 2.385 ± 0.426
2.266GlnArg: 2.266 ± 0.337
2.028GlnSer: 2.028 ± 0.279
2.028GlnThr: 2.028 ± 0.319
2.385GlnVal: 2.385 ± 0.396
0.895GlnTrp: 0.895 ± 0.216
1.312GlnTyr: 1.312 ± 0.243
0.0GlnXaa: 0.0 ± 0.0
Arg
5.427ArgAla: 5.427 ± 0.551
0.895ArgCys: 0.895 ± 0.272
4.294ArgAsp: 4.294 ± 0.548
4.89ArgGlu: 4.89 ± 0.571
1.908ArgPhe: 1.908 ± 0.408
4.473ArgGly: 4.473 ± 0.558
1.252ArgHis: 1.252 ± 0.292
3.638ArgIle: 3.638 ± 0.464
2.743ArgLys: 2.743 ± 0.448
5.01ArgLeu: 5.01 ± 0.464
2.087ArgMet: 2.087 ± 0.361
2.028ArgAsn: 2.028 ± 0.258
2.743ArgPro: 2.743 ± 0.391
2.803ArgGln: 2.803 ± 0.502
4.473ArgArg: 4.473 ± 0.531
3.519ArgSer: 3.519 ± 0.438
3.22ArgThr: 3.22 ± 0.489
3.519ArgVal: 3.519 ± 0.497
1.431ArgTrp: 1.431 ± 0.265
2.207ArgTyr: 2.207 ± 0.339
0.0ArgXaa: 0.0 ± 0.0
Ser
6.381SerAla: 6.381 ± 0.707
0.417SerCys: 0.417 ± 0.119
3.578SerAsp: 3.578 ± 0.554
3.698SerGlu: 3.698 ± 0.47
2.445SerPhe: 2.445 ± 0.351
6.322SerGly: 6.322 ± 0.963
1.193SerHis: 1.193 ± 0.266
2.505SerIle: 2.505 ± 0.424
2.385SerLys: 2.385 ± 0.374
4.95SerLeu: 4.95 ± 0.593
1.431SerMet: 1.431 ± 0.308
1.61SerAsn: 1.61 ± 0.289
2.863SerPro: 2.863 ± 0.339
2.684SerGln: 2.684 ± 0.42
3.817SerArg: 3.817 ± 0.472
3.578SerSer: 3.578 ± 0.529
3.876SerThr: 3.876 ± 0.562
3.876SerVal: 3.876 ± 0.428
1.312SerTrp: 1.312 ± 0.262
1.789SerTyr: 1.789 ± 0.302
0.0SerXaa: 0.0 ± 0.0
Thr
6.083ThrAla: 6.083 ± 0.63
0.716ThrCys: 0.716 ± 0.202
3.698ThrAsp: 3.698 ± 0.593
2.982ThrGlu: 2.982 ± 0.445
2.028ThrPhe: 2.028 ± 0.325
6.143ThrGly: 6.143 ± 0.869
0.954ThrHis: 0.954 ± 0.244
2.922ThrIle: 2.922 ± 0.456
2.266ThrLys: 2.266 ± 0.442
4.831ThrLeu: 4.831 ± 0.601
1.431ThrMet: 1.431 ± 0.278
1.551ThrAsn: 1.551 ± 0.32
4.234ThrPro: 4.234 ± 0.553
2.445ThrGln: 2.445 ± 0.395
2.982ThrArg: 2.982 ± 0.446
3.28ThrSer: 3.28 ± 0.499
3.34ThrThr: 3.34 ± 0.488
4.831ThrVal: 4.831 ± 0.541
1.014ThrTrp: 1.014 ± 0.291
1.729ThrTyr: 1.729 ± 0.264
0.0ThrXaa: 0.0 ± 0.0
Val
6.62ValAla: 6.62 ± 0.719
0.716ValCys: 0.716 ± 0.196
4.175ValAsp: 4.175 ± 0.515
3.936ValGlu: 3.936 ± 0.421
2.743ValPhe: 2.743 ± 0.533
5.01ValGly: 5.01 ± 0.532
0.716ValHis: 0.716 ± 0.219
3.936ValIle: 3.936 ± 0.479
3.34ValLys: 3.34 ± 0.406
5.666ValLeu: 5.666 ± 0.582
1.372ValMet: 1.372 ± 0.237
2.863ValAsn: 2.863 ± 0.476
3.638ValPro: 3.638 ± 0.396
2.028ValGln: 2.028 ± 0.415
5.01ValArg: 5.01 ± 0.513
4.95ValSer: 4.95 ± 0.697
4.771ValThr: 4.771 ± 0.559
4.115ValVal: 4.115 ± 0.544
1.372ValTrp: 1.372 ± 0.277
1.729ValTyr: 1.729 ± 0.314
0.0ValXaa: 0.0 ± 0.0
Trp
2.028TrpAla: 2.028 ± 0.366
0.417TrpCys: 0.417 ± 0.159
0.954TrpAsp: 0.954 ± 0.211
1.133TrpGlu: 1.133 ± 0.237
0.775TrpPhe: 0.775 ± 0.224
1.252TrpGly: 1.252 ± 0.273
0.596TrpHis: 0.596 ± 0.244
1.312TrpIle: 1.312 ± 0.273
0.954TrpLys: 0.954 ± 0.204
1.252TrpLeu: 1.252 ± 0.194
0.596TrpMet: 0.596 ± 0.164
0.537TrpAsn: 0.537 ± 0.162
0.656TrpPro: 0.656 ± 0.191
1.133TrpGln: 1.133 ± 0.323
1.133TrpArg: 1.133 ± 0.2
1.014TrpSer: 1.014 ± 0.237
0.835TrpThr: 0.835 ± 0.212
1.252TrpVal: 1.252 ± 0.299
0.537TrpTrp: 0.537 ± 0.199
0.417TrpTyr: 0.417 ± 0.153
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.147TyrAla: 2.147 ± 0.324
0.239TyrCys: 0.239 ± 0.109
2.087TyrAsp: 2.087 ± 0.341
2.266TyrGlu: 2.266 ± 0.38
0.716TyrPhe: 0.716 ± 0.217
2.445TyrGly: 2.445 ± 0.333
0.298TyrHis: 0.298 ± 0.142
1.312TyrIle: 1.312 ± 0.288
1.073TyrLys: 1.073 ± 0.263
2.624TyrLeu: 2.624 ± 0.423
0.596TyrMet: 0.596 ± 0.17
0.775TyrAsn: 0.775 ± 0.234
1.133TyrPro: 1.133 ± 0.223
1.312TyrGln: 1.312 ± 0.282
1.968TyrArg: 1.968 ± 0.403
1.431TyrSer: 1.431 ± 0.325
1.431TyrThr: 1.431 ± 0.322
2.624TyrVal: 2.624 ± 0.402
0.477TyrTrp: 0.477 ± 0.202
0.895TyrTyr: 0.895 ± 0.24
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (16769 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski