Enterobacteria phage IME_EC2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Murrayvirus; Escherichia virus EC2

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0P3B0|A0A0A0P3B0_9CAUD Uncharacterized protein OS=Enterobacteria phage IME_EC2 OX=1414766 GN=IME_EC2_58 PE=4 SV=1
MM1 pKa = 7.81EE2 pKa = 6.85DD3 pKa = 3.56INDD6 pKa = 3.98DD7 pKa = 4.15GKK9 pKa = 10.95ILLCVWEE16 pKa = 4.94ADD18 pKa = 4.11NDD20 pKa = 4.35CEE22 pKa = 4.78WMFSYY27 pKa = 10.63LVNPNGSYY35 pKa = 8.66TWNGNIYY42 pKa = 10.18LDD44 pKa = 3.76SSVKK48 pKa = 10.49EE49 pKa = 4.09EE50 pKa = 4.22LPATIRR56 pKa = 11.84DD57 pKa = 3.75EE58 pKa = 4.0KK59 pKa = 10.86HH60 pKa = 5.6LRR62 pKa = 11.84EE63 pKa = 4.18VLAFIAANII72 pKa = 3.79

Molecular weight: 8.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0P1Q7|A0A0A0P1Q7_9CAUD Putative DNA primase OS=Enterobacteria phage IME_EC2 OX=1414766 GN=IME_EC2_16 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 9.25QTLLHH7 pKa = 6.15TKK9 pKa = 7.44TNKK12 pKa = 9.88AQIVNPLLLKK22 pKa = 9.87QAHH25 pKa = 6.31ILIEE29 pKa = 3.87TAMILRR35 pKa = 11.84NRR37 pKa = 11.84LKK39 pKa = 10.06MYY41 pKa = 10.56AAGPLAKK48 pKa = 10.27AALTRR53 pKa = 11.84EE54 pKa = 4.28AVQAEE59 pKa = 3.95NDD61 pKa = 3.29ARR63 pKa = 11.84EE64 pKa = 4.71LIRR67 pKa = 11.84GAYY70 pKa = 9.32GYY72 pKa = 7.64PAHH75 pKa = 7.08

Molecular weight: 8.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12787

47

1007

213.1

23.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.168 ± 0.706

0.923 ± 0.154

6.608 ± 0.246

6.773 ± 0.409

3.12 ± 0.226

7.899 ± 0.463

1.65 ± 0.21

5.044 ± 0.286

5.553 ± 0.342

7.594 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.776 ± 0.148

4.145 ± 0.192

4.794 ± 0.18

4.684 ± 0.438

6.116 ± 0.236

5.029 ± 0.271

5.576 ± 0.331

5.998 ± 0.309

1.658 ± 0.134

2.894 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski