Amino acid dipepetide frequency for Geobacter sp. DSM 2909

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.783AlaAla: 9.783 ± 0.123
1.255AlaCys: 1.255 ± 0.031
4.917AlaAsp: 4.917 ± 0.07
6.218AlaGlu: 6.218 ± 0.084
3.618AlaPhe: 3.618 ± 0.056
8.299AlaGly: 8.299 ± 0.109
1.595AlaHis: 1.595 ± 0.039
4.859AlaIle: 4.859 ± 0.062
3.685AlaLys: 3.685 ± 0.059
9.456AlaLeu: 9.456 ± 0.104
2.358AlaMet: 2.358 ± 0.046
2.341AlaAsn: 2.341 ± 0.046
3.461AlaPro: 3.461 ± 0.068
2.239AlaGln: 2.239 ± 0.046
6.197AlaArg: 6.197 ± 0.075
5.06AlaSer: 5.06 ± 0.071
4.18AlaThr: 4.18 ± 0.064
7.575AlaVal: 7.575 ± 0.086
0.888AlaTrp: 0.888 ± 0.027
2.131AlaTyr: 2.131 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
1.15CysAla: 1.15 ± 0.032
0.263CysCys: 0.263 ± 0.015
0.668CysAsp: 0.668 ± 0.026
0.696CysGlu: 0.696 ± 0.026
0.569CysPhe: 0.569 ± 0.021
1.478CysGly: 1.478 ± 0.04
0.332CysHis: 0.332 ± 0.016
0.745CysIle: 0.745 ± 0.023
0.396CysLys: 0.396 ± 0.02
1.241CysLeu: 1.241 ± 0.036
0.289CysMet: 0.289 ± 0.014
0.411CysAsn: 0.411 ± 0.017
0.797CysPro: 0.797 ± 0.029
0.275CysGln: 0.275 ± 0.016
1.091CysArg: 1.091 ± 0.035
0.861CysSer: 0.861 ± 0.027
0.603CysThr: 0.603 ± 0.026
0.809CysVal: 0.809 ± 0.027
0.133CysTrp: 0.133 ± 0.009
0.397CysTyr: 0.397 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
4.891AspAla: 4.891 ± 0.065
0.725AspCys: 0.725 ± 0.024
2.914AspAsp: 2.914 ± 0.067
3.761AspGlu: 3.761 ± 0.062
2.602AspPhe: 2.602 ± 0.043
4.498AspGly: 4.498 ± 0.087
0.952AspHis: 0.952 ± 0.026
3.674AspIle: 3.674 ± 0.057
2.034AspLys: 2.034 ± 0.047
5.572AspLeu: 5.572 ± 0.072
1.263AspMet: 1.263 ± 0.032
1.456AspAsn: 1.456 ± 0.034
2.877AspPro: 2.877 ± 0.049
1.295AspGln: 1.295 ± 0.033
3.907AspArg: 3.907 ± 0.065
2.977AspSer: 2.977 ± 0.058
3.052AspThr: 3.052 ± 0.071
3.703AspVal: 3.703 ± 0.059
0.665AspTrp: 0.665 ± 0.026
1.79AspTyr: 1.79 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
5.994GluAla: 5.994 ± 0.077
0.719GluCys: 0.719 ± 0.025
2.99GluAsp: 2.99 ± 0.06
5.391GluGlu: 5.391 ± 0.078
2.306GluPhe: 2.306 ± 0.043
4.723GluGly: 4.723 ± 0.063
1.203GluHis: 1.203 ± 0.031
4.681GluIle: 4.681 ± 0.068
4.737GluLys: 4.737 ± 0.073
6.329GluLeu: 6.329 ± 0.073
2.088GluMet: 2.088 ± 0.041
2.392GluAsn: 2.392 ± 0.038
2.389GluPro: 2.389 ± 0.049
2.282GluGln: 2.282 ± 0.04
5.276GluArg: 5.276 ± 0.079
3.679GluSer: 3.679 ± 0.055
3.791GluThr: 3.791 ± 0.056
4.434GluVal: 4.434 ± 0.056
0.685GluTrp: 0.685 ± 0.021
1.904GluTyr: 1.904 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
3.433PheAla: 3.433 ± 0.05
0.637PheCys: 0.637 ± 0.024
2.457PheAsp: 2.457 ± 0.047
2.216PheGlu: 2.216 ± 0.038
2.054PhePhe: 2.054 ± 0.046
3.402PheGly: 3.402 ± 0.052
0.951PheHis: 0.951 ± 0.028
2.238PheIle: 2.238 ± 0.04
1.383PheLys: 1.383 ± 0.032
4.736PheLeu: 4.736 ± 0.069
0.85PheMet: 0.85 ± 0.031
1.301PheAsn: 1.301 ± 0.039
2.032PhePro: 2.032 ± 0.046
1.15PheGln: 1.15 ± 0.027
3.016PheArg: 3.016 ± 0.051
3.231PheSer: 3.231 ± 0.052
2.395PheThr: 2.395 ± 0.046
2.693PheVal: 2.693 ± 0.047
0.49PheTrp: 0.49 ± 0.025
1.13PheTyr: 1.13 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
6.584GlyAla: 6.584 ± 0.086
1.28GlyCys: 1.28 ± 0.038
4.314GlyAsp: 4.314 ± 0.098
5.452GlyGlu: 5.452 ± 0.069
3.531GlyPhe: 3.531 ± 0.049
6.697GlyGly: 6.697 ± 0.197
1.537GlyHis: 1.537 ± 0.04
5.874GlyIle: 5.874 ± 0.076
4.81GlyLys: 4.81 ± 0.058
7.209GlyLeu: 7.209 ± 0.078
2.45GlyMet: 2.45 ± 0.041
2.826GlyAsn: 2.826 ± 0.087
2.33GlyPro: 2.33 ± 0.045
1.976GlyGln: 1.976 ± 0.04
5.432GlyArg: 5.432 ± 0.078
4.768GlySer: 4.768 ± 0.078
4.678GlyThr: 4.678 ± 0.08
5.849GlyVal: 5.849 ± 0.071
0.991GlyTrp: 0.991 ± 0.029
2.711GlyTyr: 2.711 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
1.684HisAla: 1.684 ± 0.039
0.282HisCys: 0.282 ± 0.014
1.114HisAsp: 1.114 ± 0.031
1.174HisGlu: 1.174 ± 0.033
0.916HisPhe: 0.916 ± 0.03
1.762HisGly: 1.762 ± 0.043
0.509HisHis: 0.509 ± 0.024
1.045HisIle: 1.045 ± 0.028
0.631HisLys: 0.631 ± 0.02
2.071HisLeu: 2.071 ± 0.04
0.413HisMet: 0.413 ± 0.02
0.533HisAsn: 0.533 ± 0.017
1.305HisPro: 1.305 ± 0.034
0.538HisGln: 0.538 ± 0.018
1.38HisArg: 1.38 ± 0.036
0.998HisSer: 0.998 ± 0.026
0.92HisThr: 0.92 ± 0.029
1.322HisVal: 1.322 ± 0.039
0.225HisTrp: 0.225 ± 0.013
0.603HisTyr: 0.603 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
5.538IleAla: 5.538 ± 0.063
0.781IleCys: 0.781 ± 0.027
3.521IleAsp: 3.521 ± 0.049
3.842IleGlu: 3.842 ± 0.056
2.364IlePhe: 2.364 ± 0.044
4.605IleGly: 4.605 ± 0.061
1.289IleHis: 1.289 ± 0.036
3.467IleIle: 3.467 ± 0.062
2.31IleLys: 2.31 ± 0.05
6.175IleLeu: 6.175 ± 0.078
1.178IleMet: 1.178 ± 0.028
1.882IleAsn: 1.882 ± 0.043
3.285IlePro: 3.285 ± 0.057
1.531IleGln: 1.531 ± 0.035
4.346IleArg: 4.346 ± 0.061
3.904IleSer: 3.904 ± 0.059
3.444IleThr: 3.444 ± 0.053
4.241IleVal: 4.241 ± 0.058
0.494IleTrp: 0.494 ± 0.023
1.391IleTyr: 1.391 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
4.218LysAla: 4.218 ± 0.069
0.481LysCys: 0.481 ± 0.023
2.571LysAsp: 2.571 ± 0.047
3.565LysGlu: 3.565 ± 0.066
1.246LysPhe: 1.246 ± 0.03
3.944LysGly: 3.944 ± 0.056
0.78LysHis: 0.78 ± 0.025
2.94LysIle: 2.94 ± 0.049
3.387LysLys: 3.387 ± 0.073
4.012LysLeu: 4.012 ± 0.065
1.324LysMet: 1.324 ± 0.032
1.842LysAsn: 1.842 ± 0.045
2.182LysPro: 2.182 ± 0.043
1.417LysGln: 1.417 ± 0.037
3.108LysArg: 3.108 ± 0.053
2.687LysSer: 2.687 ± 0.051
2.696LysThr: 2.696 ± 0.042
3.328LysVal: 3.328 ± 0.058
0.421LysTrp: 0.421 ± 0.019
1.326LysTyr: 1.326 ± 0.039
0.0LysXaa: 0.0 ± 0.0
Leu
10.114LeuAla: 10.114 ± 0.098
1.309LeuCys: 1.309 ± 0.034
5.602LeuAsp: 5.602 ± 0.076
6.688LeuGlu: 6.688 ± 0.082
4.448LeuPhe: 4.448 ± 0.061
6.963LeuGly: 6.963 ± 0.075
2.001LeuHis: 2.001 ± 0.04
5.035LeuIle: 5.035 ± 0.064
5.015LeuLys: 5.015 ± 0.062
10.787LeuLeu: 10.787 ± 0.117
2.228LeuMet: 2.228 ± 0.047
2.983LeuAsn: 2.983 ± 0.05
5.048LeuPro: 5.048 ± 0.063
2.752LeuGln: 2.752 ± 0.056
6.985LeuArg: 6.985 ± 0.083
6.952LeuSer: 6.952 ± 0.074
5.232LeuThr: 5.232 ± 0.069
7.179LeuVal: 7.179 ± 0.076
0.924LeuTrp: 0.924 ± 0.032
2.617LeuTyr: 2.617 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
2.374MetAla: 2.374 ± 0.046
0.206MetCys: 0.206 ± 0.012
1.392MetAsp: 1.392 ± 0.034
1.893MetGlu: 1.893 ± 0.041
0.677MetPhe: 0.677 ± 0.025
1.933MetGly: 1.933 ± 0.036
0.421MetHis: 0.421 ± 0.017
1.334MetIle: 1.334 ± 0.035
1.777MetLys: 1.777 ± 0.037
2.159MetLeu: 2.159 ± 0.039
0.591MetMet: 0.591 ± 0.025
1.009MetAsn: 1.009 ± 0.029
1.158MetPro: 1.158 ± 0.031
0.677MetGln: 0.677 ± 0.022
1.521MetArg: 1.521 ± 0.03
1.479MetSer: 1.479 ± 0.031
1.518MetThr: 1.518 ± 0.032
1.76MetVal: 1.76 ± 0.045
0.167MetTrp: 0.167 ± 0.012
0.446MetTyr: 0.446 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
2.618AsnAla: 2.618 ± 0.053
0.413AsnCys: 0.413 ± 0.019
1.619AsnAsp: 1.619 ± 0.07
1.711AsnGlu: 1.711 ± 0.035
1.148AsnPhe: 1.148 ± 0.028
2.727AsnGly: 2.727 ± 0.059
0.637AsnHis: 0.637 ± 0.02
2.09AsnIle: 2.09 ± 0.045
1.174AsnLys: 1.174 ± 0.036
3.47AsnLeu: 3.47 ± 0.06
0.672AsnMet: 0.672 ± 0.024
1.042AsnAsn: 1.042 ± 0.033
2.154AsnPro: 2.154 ± 0.038
0.859AsnGln: 0.859 ± 0.03
2.375AsnArg: 2.375 ± 0.046
1.743AsnSer: 1.743 ± 0.045
1.485AsnThr: 1.485 ± 0.037
2.04AsnVal: 2.04 ± 0.039
0.359AsnTrp: 0.359 ± 0.014
0.938AsnTyr: 0.938 ± 0.03
0.0AsnXaa: 0.0 ± 0.0
Pro
4.289ProAla: 4.289 ± 0.058
0.594ProCys: 0.594 ± 0.023
3.251ProAsp: 3.251 ± 0.056
3.94ProGlu: 3.94 ± 0.066
2.172ProPhe: 2.172 ± 0.04
3.98ProGly: 3.98 ± 0.068
0.914ProHis: 0.914 ± 0.029
2.029ProIle: 2.029 ± 0.037
1.723ProLys: 1.723 ± 0.043
4.556ProLeu: 4.556 ± 0.067
0.981ProMet: 0.981 ± 0.026
1.147ProAsn: 1.147 ± 0.029
2.317ProPro: 2.317 ± 0.062
1.166ProGln: 1.166 ± 0.031
2.398ProArg: 2.398 ± 0.048
2.836ProSer: 2.836 ± 0.05
2.042ProThr: 2.042 ± 0.039
4.102ProVal: 4.102 ± 0.063
0.549ProTrp: 0.549 ± 0.021
1.299ProTyr: 1.299 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
2.659GlnAla: 2.659 ± 0.048
0.301GlnCys: 0.301 ± 0.019
1.239GlnAsp: 1.239 ± 0.028
1.896GlnGlu: 1.896 ± 0.04
0.956GlnPhe: 0.956 ± 0.024
2.284GlnGly: 2.284 ± 0.039
0.461GlnHis: 0.461 ± 0.019
1.55GlnIle: 1.55 ± 0.043
1.493GlnLys: 1.493 ± 0.041
2.426GlnLeu: 2.426 ± 0.047
0.757GlnMet: 0.757 ± 0.023
0.862GlnAsn: 0.862 ± 0.031
1.186GlnPro: 1.186 ± 0.031
1.019GlnGln: 1.019 ± 0.034
1.848GlnArg: 1.848 ± 0.04
1.637GlnSer: 1.637 ± 0.038
1.411GlnThr: 1.411 ± 0.035
2.16GlnVal: 2.16 ± 0.04
0.328GlnTrp: 0.328 ± 0.017
0.763GlnTyr: 0.763 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
5.054ArgAla: 5.054 ± 0.073
0.866ArgCys: 0.866 ± 0.026
4.08ArgAsp: 4.08 ± 0.056
5.968ArgGlu: 5.968 ± 0.073
3.248ArgPhe: 3.248 ± 0.056
4.516ArgGly: 4.516 ± 0.06
1.46ArgHis: 1.46 ± 0.036
4.913ArgIle: 4.913 ± 0.066
3.871ArgLys: 3.871 ± 0.053
7.104ArgLeu: 7.104 ± 0.089
2.0ArgMet: 2.0 ± 0.038
2.484ArgAsn: 2.484 ± 0.049
2.528ArgPro: 2.528 ± 0.05
2.157ArgGln: 2.157 ± 0.042
4.964ArgArg: 4.964 ± 0.073
3.665ArgSer: 3.665 ± 0.058
3.283ArgThr: 3.283 ± 0.052
4.675ArgVal: 4.675 ± 0.061
0.747ArgTrp: 0.747 ± 0.025
2.23ArgTyr: 2.23 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
5.246SerAla: 5.246 ± 0.071
0.887SerCys: 0.887 ± 0.03
3.059SerAsp: 3.059 ± 0.049
3.39SerGlu: 3.39 ± 0.06
2.925SerPhe: 2.925 ± 0.049
6.013SerGly: 6.013 ± 0.096
1.201SerHis: 1.201 ± 0.027
3.543SerIle: 3.543 ± 0.058
2.07SerLys: 2.07 ± 0.044
6.873SerLeu: 6.873 ± 0.075
1.394SerMet: 1.394 ± 0.036
1.608SerAsn: 1.608 ± 0.046
3.078SerPro: 3.078 ± 0.051
1.543SerGln: 1.543 ± 0.035
4.251SerArg: 4.251 ± 0.067
3.83SerSer: 3.83 ± 0.072
2.838SerThr: 2.838 ± 0.051
4.369SerVal: 4.369 ± 0.07
0.733SerTrp: 0.733 ± 0.025
1.68SerTyr: 1.68 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
5.059ThrAla: 5.059 ± 0.077
0.614ThrCys: 0.614 ± 0.021
2.798ThrAsp: 2.798 ± 0.047
3.057ThrGlu: 3.057 ± 0.053
2.172ThrPhe: 2.172 ± 0.044
5.213ThrGly: 5.213 ± 0.072
0.972ThrHis: 0.972 ± 0.027
3.341ThrIle: 3.341 ± 0.057
1.898ThrLys: 1.898 ± 0.039
5.493ThrLeu: 5.493 ± 0.075
1.153ThrMet: 1.153 ± 0.026
1.506ThrAsn: 1.506 ± 0.04
2.833ThrPro: 2.833 ± 0.049
1.15ThrGln: 1.15 ± 0.033
3.141ThrArg: 3.141 ± 0.048
2.916ThrSer: 2.916 ± 0.058
2.813ThrThr: 2.813 ± 0.065
4.699ThrVal: 4.699 ± 0.064
0.502ThrTrp: 0.502 ± 0.019
1.343ThrTyr: 1.343 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
6.402ValAla: 6.402 ± 0.081
1.012ValCys: 1.012 ± 0.036
3.865ValAsp: 3.865 ± 0.063
4.909ValGlu: 4.909 ± 0.059
3.095ValPhe: 3.095 ± 0.044
5.086ValGly: 5.086 ± 0.076
1.335ValHis: 1.335 ± 0.033
4.414ValIle: 4.414 ± 0.061
3.531ValLys: 3.531 ± 0.055
6.97ValLeu: 6.97 ± 0.09
1.764ValMet: 1.764 ± 0.036
2.318ValAsn: 2.318 ± 0.046
3.444ValPro: 3.444 ± 0.055
1.861ValGln: 1.861 ± 0.037
5.222ValArg: 5.222 ± 0.069
4.997ValSer: 4.997 ± 0.069
4.398ValThr: 4.398 ± 0.06
5.765ValVal: 5.765 ± 0.078
0.691ValTrp: 0.691 ± 0.023
1.907ValTyr: 1.907 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
0.74TrpAla: 0.74 ± 0.024
0.146TrpCys: 0.146 ± 0.01
0.595TrpAsp: 0.595 ± 0.022
0.701TrpGlu: 0.701 ± 0.026
0.483TrpPhe: 0.483 ± 0.018
0.767TrpGly: 0.767 ± 0.023
0.234TrpHis: 0.234 ± 0.013
0.582TrpIle: 0.582 ± 0.021
0.597TrpLys: 0.597 ± 0.022
1.062TrpLeu: 1.062 ± 0.036
0.27TrpMet: 0.27 ± 0.015
0.429TrpAsn: 0.429 ± 0.02
0.374TrpPro: 0.374 ± 0.017
0.414TrpGln: 0.414 ± 0.019
0.821TrpArg: 0.821 ± 0.028
0.622TrpSer: 0.622 ± 0.025
0.483TrpThr: 0.483 ± 0.02
0.644TrpVal: 0.644 ± 0.023
0.149TrpTrp: 0.149 ± 0.01
0.347TrpTyr: 0.347 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.284TyrAla: 2.284 ± 0.044
0.432TyrCys: 0.432 ± 0.016
1.641TyrAsp: 1.641 ± 0.04
1.526TyrGlu: 1.526 ± 0.032
1.263TyrPhe: 1.263 ± 0.036
2.411TyrGly: 2.411 ± 0.046
0.642TyrHis: 0.642 ± 0.023
1.238TyrIle: 1.238 ± 0.032
0.954TyrLys: 0.954 ± 0.029
3.16TyrLeu: 3.16 ± 0.054
0.468TyrMet: 0.468 ± 0.02
0.898TyrAsn: 0.898 ± 0.032
1.506TyrPro: 1.506 ± 0.04
0.934TyrGln: 0.934 ± 0.026
2.535TyrArg: 2.535 ± 0.052
1.69TyrSer: 1.69 ± 0.041
1.413TyrThr: 1.413 ± 0.035
1.639TyrVal: 1.639 ± 0.035
0.319TyrTrp: 0.319 ± 0.016
0.94TyrTyr: 0.94 ± 0.031
0.001TyrXaa: 0.001 ± 0.001
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.001XaaSer: 0.001 ± 0.001
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.002XaaXaa: 0.002 ± 0.002
Statistics based on 4159 proteins (1352630 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski