Amino acid dipepetide frequency for Pseudogymnoascus sp. 24MN13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.979AlaAla: 9.979 ± 0.071	1.061AlaCys: 1.061 ± 0.017	4.469AlaAsp: 4.469 ± 0.031	5.398AlaGlu: 5.398 ± 0.055	3.159AlaPhe: 3.159 ± 0.025	6.166AlaGly: 6.166 ± 0.036	1.741AlaHis: 1.741 ± 0.022	4.628AlaIle: 4.628 ± 0.032	4.384AlaLys: 4.384 ± 0.035	7.872AlaLeu: 7.872 ± 0.042	2.001AlaMet: 2.001 ± 0.023	3.171AlaAsn: 3.171 ± 0.028	5.134AlaPro: 5.134 ± 0.043	3.382AlaGln: 3.382 ± 0.035	4.939AlaArg: 4.939 ± 0.032	7.193AlaSer: 7.193 ± 0.044	5.664AlaThr: 5.664 ± 0.038	5.577AlaVal: 5.577 ± 0.033	1.129AlaTrp: 1.129 ± 0.017	2.208AlaTyr: 2.208 ± 0.024	0.0AlaXaa: 0.0 ± 0.0
Cys	0.987CysAla: 0.987 ± 0.017	0.239CysCys: 0.239 ± 0.008	0.66CysAsp: 0.66 ± 0.012	0.579CysGlu: 0.579 ± 0.011	0.532CysPhe: 0.532 ± 0.012	0.969CysGly: 0.969 ± 0.016	0.308CysHis: 0.308 ± 0.009	0.697CysIle: 0.697 ± 0.014	0.499CysLys: 0.499 ± 0.011	1.175CysLeu: 1.175 ± 0.019	0.271CysMet: 0.271 ± 0.008	0.427CysAsn: 0.427 ± 0.01	0.624CysPro: 0.624 ± 0.013	0.412CysGln: 0.412 ± 0.009	0.657CysArg: 0.657 ± 0.013	0.889CysSer: 0.889 ± 0.015	0.687CysThr: 0.687 ± 0.013	0.759CysVal: 0.759 ± 0.012	0.197CysTrp: 0.197 ± 0.007	0.364CysTyr: 0.364 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	4.918AspAla: 4.918 ± 0.035	0.6AspCys: 0.6 ± 0.013	4.197AspAsp: 4.197 ± 0.044	4.52AspGlu: 4.52 ± 0.044	2.246AspPhe: 2.246 ± 0.024	4.269AspGly: 4.269 ± 0.032	1.132AspHis: 1.132 ± 0.015	3.241AspIle: 3.241 ± 0.026	2.482AspLys: 2.482 ± 0.027	4.96AspLeu: 4.96 ± 0.032	1.272AspMet: 1.272 ± 0.016	1.991AspAsn: 1.991 ± 0.019	3.076AspPro: 3.076 ± 0.027	1.719AspGln: 1.719 ± 0.02	2.867AspArg: 2.867 ± 0.027	4.074AspSer: 4.074 ± 0.037	3.016AspThr: 3.016 ± 0.029	3.75AspVal: 3.75 ± 0.03	0.859AspTrp: 0.859 ± 0.014	1.614AspTyr: 1.614 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	5.679GluAla: 5.679 ± 0.049	0.608GluCys: 0.608 ± 0.013	4.216GluAsp: 4.216 ± 0.043	5.657GluGlu: 5.657 ± 0.062	1.988GluPhe: 1.988 ± 0.021	4.064GluGly: 4.064 ± 0.034	1.282GluHis: 1.282 ± 0.017	3.16GluIle: 3.16 ± 0.028	3.77GluLys: 3.77 ± 0.032	5.147GluLeu: 5.147 ± 0.039	1.521GluMet: 1.521 ± 0.019	2.276GluAsn: 2.276 ± 0.022	2.667GluPro: 2.667 ± 0.034	2.317GluGln: 2.317 ± 0.026	3.858GluArg: 3.858 ± 0.036	4.241GluSer: 4.241 ± 0.033	3.47GluThr: 3.47 ± 0.028	3.814GluVal: 3.814 ± 0.033	0.879GluTrp: 0.879 ± 0.016	1.709GluTyr: 1.709 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	3.12PheAla: 3.12 ± 0.028	0.537PheCys: 0.537 ± 0.011	2.278PheAsp: 2.278 ± 0.024	2.196PheGlu: 2.196 ± 0.024	1.604PhePhe: 1.604 ± 0.025	3.013PheGly: 3.013 ± 0.032	0.833PheHis: 0.833 ± 0.013	1.909PheIle: 1.909 ± 0.021	1.594PheLys: 1.594 ± 0.019	3.353PheLeu: 3.353 ± 0.029	0.833PheMet: 0.833 ± 0.013	1.457PheAsn: 1.457 ± 0.016	1.881PhePro: 1.881 ± 0.02	1.371PheGln: 1.371 ± 0.02	1.792PheArg: 1.792 ± 0.022	2.907PheSer: 2.907 ± 0.026	2.159PheThr: 2.159 ± 0.025	2.372PheVal: 2.372 ± 0.023	0.638PheTrp: 0.638 ± 0.013	1.126PheTyr: 1.126 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	5.835GlyAla: 5.835 ± 0.042	0.903GlyCys: 0.903 ± 0.015	3.817GlyAsp: 3.817 ± 0.028	3.958GlyGlu: 3.958 ± 0.032	2.905GlyPhe: 2.905 ± 0.026	6.496GlyGly: 6.496 ± 0.058	1.65GlyHis: 1.65 ± 0.021	3.788GlyIle: 3.788 ± 0.033	3.764GlyLys: 3.764 ± 0.036	6.127GlyLeu: 6.127 ± 0.038	1.718GlyMet: 1.718 ± 0.021	2.734GlyAsn: 2.734 ± 0.025	3.365GlyPro: 3.365 ± 0.033	2.564GlyGln: 2.564 ± 0.032	4.125GlyArg: 4.125 ± 0.035	5.785GlySer: 5.785 ± 0.04	4.141GlyThr: 4.141 ± 0.033	4.605GlyVal: 4.605 ± 0.034	1.177GlyTrp: 1.177 ± 0.018	2.201GlyTyr: 2.201 ± 0.023	0.0GlyXaa: 0.0 ± 0.0
His	1.736HisAla: 1.736 ± 0.018	0.294HisCys: 0.294 ± 0.008	1.216HisAsp: 1.216 ± 0.018	1.225HisGlu: 1.225 ± 0.017	0.869HisPhe: 0.869 ± 0.014	1.644HisGly: 1.644 ± 0.02	0.704HisHis: 0.704 ± 0.013	1.215HisIle: 1.215 ± 0.015	0.928HisLys: 0.928 ± 0.014	2.092HisLeu: 2.092 ± 0.021	0.459HisMet: 0.459 ± 0.009	0.852HisAsn: 0.852 ± 0.014	1.524HisPro: 1.524 ± 0.019	0.888HisGln: 0.888 ± 0.015	1.382HisArg: 1.382 ± 0.019	1.638HisSer: 1.638 ± 0.02	1.261HisThr: 1.261 ± 0.017	1.343HisVal: 1.343 ± 0.016	0.322HisTrp: 0.322 ± 0.008	0.675HisTyr: 0.675 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	4.532IleAla: 4.532 ± 0.038	0.782IleCys: 0.782 ± 0.013	2.985IleAsp: 2.985 ± 0.026	3.009IleGlu: 3.009 ± 0.027	2.09IlePhe: 2.09 ± 0.027	3.444IleGly: 3.444 ± 0.03	1.148IleHis: 1.148 ± 0.014	2.808IleIle: 2.808 ± 0.032	2.375IleLys: 2.375 ± 0.023	4.674IleLeu: 4.674 ± 0.037	1.078IleMet: 1.078 ± 0.016	1.974IleAsn: 1.974 ± 0.02	3.126IlePro: 3.126 ± 0.024	1.915IleGln: 1.915 ± 0.021	2.711IleArg: 2.711 ± 0.026	4.078IleSer: 4.078 ± 0.032	3.152IleThr: 3.152 ± 0.028	3.308IleVal: 3.308 ± 0.03	0.719IleTrp: 0.719 ± 0.012	1.534IleTyr: 1.534 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	4.577LysAla: 4.577 ± 0.041	0.499LysCys: 0.499 ± 0.011	2.868LysAsp: 2.868 ± 0.028	3.545LysGlu: 3.545 ± 0.037	1.568LysPhe: 1.568 ± 0.019	3.248LysGly: 3.248 ± 0.03	1.074LysHis: 1.074 ± 0.017	2.455LysIle: 2.455 ± 0.024	3.431LysLys: 3.431 ± 0.052	4.248LysLeu: 4.248 ± 0.031	1.063LysMet: 1.063 ± 0.013	1.865LysAsn: 1.865 ± 0.021	2.715LysPro: 2.715 ± 0.028	1.757LysGln: 1.757 ± 0.021	3.259LysArg: 3.259 ± 0.031	3.69LysSer: 3.69 ± 0.032	2.952LysThr: 2.952 ± 0.026	2.955LysVal: 2.955 ± 0.024	0.704LysTrp: 0.704 ± 0.012	1.452LysTyr: 1.452 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	7.893LeuAla: 7.893 ± 0.048	1.137LeuCys: 1.137 ± 0.016	5.111LeuAsp: 5.111 ± 0.034	5.45LeuGlu: 5.45 ± 0.034	3.258LeuPhe: 3.258 ± 0.032	6.009LeuGly: 6.009 ± 0.041	2.116LeuHis: 2.116 ± 0.023	4.085LeuIle: 4.085 ± 0.035	4.374LeuLys: 4.374 ± 0.033	8.329LeuLeu: 8.329 ± 0.056	1.793LeuMet: 1.793 ± 0.018	3.147LeuAsn: 3.147 ± 0.025	5.344LeuPro: 5.344 ± 0.039	3.661LeuGln: 3.661 ± 0.033	5.345LeuArg: 5.345 ± 0.04	7.135LeuSer: 7.135 ± 0.042	4.831LeuThr: 4.831 ± 0.034	5.275LeuVal: 5.275 ± 0.037	1.177LeuTrp: 1.177 ± 0.018	2.319LeuTyr: 2.319 ± 0.025	0.0LeuXaa: 0.0 ± 0.0
Met	2.272MetAla: 2.272 ± 0.022	0.229MetCys: 0.229 ± 0.007	1.271MetAsp: 1.271 ± 0.02	1.354MetGlu: 1.354 ± 0.019	0.781MetPhe: 0.781 ± 0.014	1.603MetGly: 1.603 ± 0.021	0.485MetHis: 0.485 ± 0.01	1.012MetIle: 1.012 ± 0.015	1.093MetLys: 1.093 ± 0.014	1.835MetLeu: 1.835 ± 0.021	0.59MetMet: 0.59 ± 0.012	0.825MetAsn: 0.825 ± 0.015	1.221MetPro: 1.221 ± 0.015	0.817MetGln: 0.817 ± 0.014	1.228MetArg: 1.228 ± 0.017	1.882MetSer: 1.882 ± 0.021	1.246MetThr: 1.246 ± 0.016	1.354MetVal: 1.354 ± 0.017	0.274MetTrp: 0.274 ± 0.008	0.526MetTyr: 0.526 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	3.243AsnAla: 3.243 ± 0.033	0.46AsnCys: 0.46 ± 0.011	2.003AsnAsp: 2.003 ± 0.019	2.051AsnGlu: 2.051 ± 0.023	1.464AsnPhe: 1.464 ± 0.018	3.209AsnGly: 3.209 ± 0.035	0.816AsnHis: 0.816 ± 0.013	2.269AsnIle: 2.269 ± 0.021	1.631AsnLys: 1.631 ± 0.02	3.304AsnLeu: 3.304 ± 0.029	0.858AsnMet: 0.858 ± 0.015	1.63AsnAsn: 1.63 ± 0.021	2.46AsnPro: 2.46 ± 0.024	1.315AsnGln: 1.315 ± 0.018	1.881AsnArg: 1.881 ± 0.022	2.901AsnSer: 2.901 ± 0.027	2.286AsnThr: 2.286 ± 0.023	2.327AsnVal: 2.327 ± 0.025	0.565AsnTrp: 0.565 ± 0.012	1.151AsnTyr: 1.151 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	5.299ProAla: 5.299 ± 0.053	0.467ProCys: 0.467 ± 0.011	2.951ProAsp: 2.951 ± 0.027	3.652ProGlu: 3.652 ± 0.034	1.96ProPhe: 1.96 ± 0.022	3.892ProGly: 3.892 ± 0.035	1.244ProHis: 1.244 ± 0.016	2.639ProIle: 2.639 ± 0.026	2.763ProLys: 2.763 ± 0.027	4.681ProLeu: 4.681 ± 0.032	1.053ProMet: 1.053 ± 0.017	2.24ProAsn: 2.24 ± 0.027	4.859ProPro: 4.859 ± 0.054	2.366ProGln: 2.366 ± 0.029	3.296ProArg: 3.296 ± 0.028	5.634ProSer: 5.634 ± 0.043	4.172ProThr: 4.172 ± 0.036	3.477ProVal: 3.477 ± 0.033	0.713ProTrp: 0.713 ± 0.014	1.452ProTyr: 1.452 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	3.315GlnAla: 3.315 ± 0.028	0.42GlnCys: 0.42 ± 0.01	1.944GlnAsp: 1.944 ± 0.023	2.276GlnGlu: 2.276 ± 0.023	1.257GlnPhe: 1.257 ± 0.015	2.464GlnGly: 2.464 ± 0.027	0.953GlnHis: 0.953 ± 0.015	1.888GlnIle: 1.888 ± 0.02	1.941GlnLys: 1.941 ± 0.023	3.359GlnLeu: 3.359 ± 0.026	0.863GlnMet: 0.863 ± 0.014	1.561GlnAsn: 1.561 ± 0.019	2.325GlnPro: 2.325 ± 0.032	2.356GlnGln: 2.356 ± 0.052	2.399GlnArg: 2.399 ± 0.027	2.912GlnSer: 2.912 ± 0.029	2.21GlnThr: 2.21 ± 0.024	2.054GlnVal: 2.054 ± 0.022	0.557GlnTrp: 0.557 ± 0.012	1.171GlnTyr: 1.171 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	4.702ArgAla: 4.702 ± 0.039	0.661ArgCys: 0.661 ± 0.013	3.29ArgAsp: 3.29 ± 0.03	3.76ArgGlu: 3.76 ± 0.04	1.955ArgPhe: 1.955 ± 0.022	3.814ArgGly: 3.814 ± 0.034	1.443ArgHis: 1.443 ± 0.017	2.825ArgIle: 2.825 ± 0.023	3.264ArgLys: 3.264 ± 0.031	5.06ArgLeu: 5.06 ± 0.034	1.26ArgMet: 1.26 ± 0.018	2.181ArgAsn: 2.181 ± 0.024	3.268ArgPro: 3.268 ± 0.033	2.373ArgGln: 2.373 ± 0.023	4.815ArgArg: 4.815 ± 0.039	4.373ArgSer: 4.373 ± 0.036	3.149ArgThr: 3.149 ± 0.029	3.219ArgVal: 3.219 ± 0.026	0.831ArgTrp: 0.831 ± 0.013	1.552ArgTyr: 1.552 ± 0.019	0.0ArgXaa: 0.0 ± 0.0
Ser	6.639SerAla: 6.639 ± 0.041	0.864SerCys: 0.864 ± 0.014	4.175SerAsp: 4.175 ± 0.032	4.026SerGlu: 4.026 ± 0.035	2.941SerPhe: 2.941 ± 0.028	5.664SerGly: 5.664 ± 0.041	1.797SerHis: 1.797 ± 0.02	4.192SerIle: 4.192 ± 0.035	3.837SerLys: 3.837 ± 0.036	7.053SerLeu: 7.053 ± 0.038	1.702SerMet: 1.702 ± 0.021	3.08SerAsn: 3.08 ± 0.028	5.332SerPro: 5.332 ± 0.045	3.111SerGln: 3.111 ± 0.032	4.62SerArg: 4.62 ± 0.035	8.154SerSer: 8.154 ± 0.062	5.592SerThr: 5.592 ± 0.038	4.516SerVal: 4.516 ± 0.033	1.101SerTrp: 1.101 ± 0.018	2.113SerTyr: 2.113 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	5.458ThrAla: 5.458 ± 0.04	0.779ThrCys: 0.779 ± 0.013	2.973ThrAsp: 2.973 ± 0.025	3.199ThrGlu: 3.199 ± 0.03	2.276ThrPhe: 2.276 ± 0.023	4.331ThrGly: 4.331 ± 0.036	1.243ThrHis: 1.243 ± 0.017	3.319ThrIle: 3.319 ± 0.032	2.781ThrLys: 2.781 ± 0.024	5.195ThrLeu: 5.195 ± 0.037	1.216ThrMet: 1.216 ± 0.017	2.273ThrAsn: 2.273 ± 0.022	4.403ThrPro: 4.403 ± 0.039	2.043ThrGln: 2.043 ± 0.02	2.986ThrArg: 2.986 ± 0.027	5.379ThrSer: 5.379 ± 0.042	4.616ThrThr: 4.616 ± 0.043	3.756ThrVal: 3.756 ± 0.03	0.877ThrTrp: 0.877 ± 0.015	1.689ThrTyr: 1.689 ± 0.018	0.0ThrXaa: 0.0 ± 0.0
Val	5.681ValAla: 5.681 ± 0.042	0.782ValCys: 0.782 ± 0.014	3.806ValAsp: 3.806 ± 0.029	4.066ValGlu: 4.066 ± 0.037	2.379ValPhe: 2.379 ± 0.024	4.215ValGly: 4.215 ± 0.036	1.286ValHis: 1.286 ± 0.016	3.078ValIle: 3.078 ± 0.028	3.045ValLys: 3.045 ± 0.025	5.48ValLeu: 5.48 ± 0.041	1.333ValMet: 1.333 ± 0.018	2.279ValAsn: 2.279 ± 0.023	3.447ValPro: 3.447 ± 0.029	2.24ValGln: 2.24 ± 0.021	3.229ValArg: 3.229 ± 0.026	4.491ValSer: 4.491 ± 0.035	3.584ValThr: 3.584 ± 0.028	4.555ValVal: 4.555 ± 0.038	0.852ValTrp: 0.852 ± 0.016	1.716ValTyr: 1.716 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	1.181TrpAla: 1.181 ± 0.017	0.187TrpCys: 0.187 ± 0.006	0.881TrpAsp: 0.881 ± 0.015	0.848TrpGlu: 0.848 ± 0.012	0.523TrpPhe: 0.523 ± 0.01	0.98TrpGly: 0.98 ± 0.017	0.325TrpHis: 0.325 ± 0.008	0.734TrpIle: 0.734 ± 0.013	0.798TrpLys: 0.798 ± 0.014	1.248TrpLeu: 1.248 ± 0.017	0.373TrpMet: 0.373 ± 0.009	0.635TrpAsn: 0.635 ± 0.011	0.591TrpPro: 0.591 ± 0.013	0.523TrpGln: 0.523 ± 0.012	0.895TrpArg: 0.895 ± 0.015	1.037TrpSer: 1.037 ± 0.017	0.915TrpThr: 0.915 ± 0.017	0.895TrpVal: 0.895 ± 0.015	0.272TrpTrp: 0.272 ± 0.008	0.434TrpTyr: 0.434 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.207TyrAla: 2.207 ± 0.023	0.423TyrCys: 0.423 ± 0.009	1.694TyrAsp: 1.694 ± 0.021	1.553TyrGlu: 1.553 ± 0.017	1.217TyrPhe: 1.217 ± 0.016	2.163TyrGly: 2.163 ± 0.025	0.687TyrHis: 0.687 ± 0.012	1.501TyrIle: 1.501 ± 0.02	1.172TyrLys: 1.172 ± 0.016	2.593TyrLeu: 2.593 ± 0.025	0.64TyrMet: 0.64 ± 0.012	1.228TyrAsn: 1.228 ± 0.018	1.448TyrPro: 1.448 ± 0.018	1.095TyrGln: 1.095 ± 0.016	1.51TyrArg: 1.51 ± 0.017	2.12TyrSer: 2.12 ± 0.024	1.687TyrThr: 1.687 ± 0.021	1.636TyrVal: 1.636 ± 0.02	0.432TyrTrp: 0.432 ± 0.009	0.95TyrTyr: 0.95 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 10229 proteins (4827133 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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