Amino acid dipepetide frequency for Escherichia phage EcS1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.302AlaAla: 5.302 ± 0.368
0.457AlaCys: 0.457 ± 0.079
4.187AlaAsp: 4.187 ± 0.261
4.589AlaGlu: 4.589 ± 0.372
2.45AlaPhe: 2.45 ± 0.218
4.955AlaGly: 4.955 ± 0.423
1.335AlaHis: 1.335 ± 0.151
4.79AlaIle: 4.79 ± 0.263
5.064AlaLys: 5.064 ± 0.329
6.015AlaLeu: 6.015 ± 0.359
2.103AlaMet: 2.103 ± 0.19
3.62AlaAsn: 3.62 ± 0.293
2.523AlaPro: 2.523 ± 0.231
2.468AlaGln: 2.468 ± 0.204
3.145AlaArg: 3.145 ± 0.262
4.424AlaSer: 4.424 ± 0.295
4.077AlaThr: 4.077 ± 0.439
4.278AlaVal: 4.278 ± 0.323
1.17AlaTrp: 1.17 ± 0.178
2.45AlaTyr: 2.45 ± 0.219
0.0AlaXaa: 0.0 ± 0.0
Cys
0.841CysAla: 0.841 ± 0.148
0.201CysCys: 0.201 ± 0.054
0.731CysAsp: 0.731 ± 0.096
0.75CysGlu: 0.75 ± 0.123
0.457CysPhe: 0.457 ± 0.1
0.695CysGly: 0.695 ± 0.117
0.293CysHis: 0.293 ± 0.064
0.75CysIle: 0.75 ± 0.122
0.731CysLys: 0.731 ± 0.13
0.603CysLeu: 0.603 ± 0.099
0.421CysMet: 0.421 ± 0.081
0.53CysAsn: 0.53 ± 0.117
0.457CysPro: 0.457 ± 0.093
0.347CysGln: 0.347 ± 0.09
0.64CysArg: 0.64 ± 0.122
0.969CysSer: 0.969 ± 0.137
0.676CysThr: 0.676 ± 0.107
0.713CysVal: 0.713 ± 0.122
0.128CysTrp: 0.128 ± 0.052
0.384CysTyr: 0.384 ± 0.077
0.0CysXaa: 0.0 ± 0.0
Asp
4.041AspAla: 4.041 ± 0.26
0.713AspCys: 0.713 ± 0.092
4.041AspAsp: 4.041 ± 0.282
4.644AspGlu: 4.644 ± 0.32
3.675AspPhe: 3.675 ± 0.27
4.845AspGly: 4.845 ± 0.33
0.896AspHis: 0.896 ± 0.134
4.699AspIle: 4.699 ± 0.294
4.589AspLys: 4.589 ± 0.32
4.882AspLeu: 4.882 ± 0.317
1.956AspMet: 1.956 ± 0.189
3.072AspAsn: 3.072 ± 0.228
2.413AspPro: 2.413 ± 0.193
1.444AspGln: 1.444 ± 0.183
2.432AspArg: 2.432 ± 0.209
4.205AspSer: 4.205 ± 0.221
3.748AspThr: 3.748 ± 0.276
3.949AspVal: 3.949 ± 0.28
1.17AspTrp: 1.17 ± 0.146
3.053AspTyr: 3.053 ± 0.257
0.0AspXaa: 0.0 ± 0.0
Glu
5.631GluAla: 5.631 ± 0.445
0.878GluCys: 0.878 ± 0.11
4.077GluAsp: 4.077 ± 0.302
4.424GluGlu: 4.424 ± 0.344
3.273GluPhe: 3.273 ± 0.187
3.565GluGly: 3.565 ± 0.232
1.554GluHis: 1.554 ± 0.157
5.467GluIle: 5.467 ± 0.358
4.37GluLys: 4.37 ± 0.301
6.838GluLeu: 6.838 ± 0.34
2.267GluMet: 2.267 ± 0.215
3.053GluAsn: 3.053 ± 0.221
1.92GluPro: 1.92 ± 0.202
2.541GluGln: 2.541 ± 0.206
2.889GluArg: 2.889 ± 0.224
3.785GluSer: 3.785 ± 0.294
4.168GluThr: 4.168 ± 0.311
5.302GluVal: 5.302 ± 0.336
1.079GluTrp: 1.079 ± 0.154
3.09GluTyr: 3.09 ± 0.249
0.0GluXaa: 0.0 ± 0.0
Phe
2.907PheAla: 2.907 ± 0.213
0.384PheCys: 0.384 ± 0.082
3.072PheAsp: 3.072 ± 0.247
3.602PheGlu: 3.602 ± 0.285
1.627PhePhe: 1.627 ± 0.206
3.017PheGly: 3.017 ± 0.241
0.859PheHis: 0.859 ± 0.124
3.273PheIle: 3.273 ± 0.246
3.894PheLys: 3.894 ± 0.249
2.688PheLeu: 2.688 ± 0.222
1.134PheMet: 1.134 ± 0.151
2.944PheAsn: 2.944 ± 0.243
1.097PhePro: 1.097 ± 0.134
1.262PheGln: 1.262 ± 0.142
1.591PheArg: 1.591 ± 0.153
2.669PheSer: 2.669 ± 0.204
2.907PheThr: 2.907 ± 0.208
2.742PheVal: 2.742 ± 0.196
0.64PheTrp: 0.64 ± 0.11
1.408PheTyr: 1.408 ± 0.162
0.0PheXaa: 0.0 ± 0.0
Gly
3.529GlyAla: 3.529 ± 0.333
0.731GlyCys: 0.731 ± 0.134
3.949GlyAsp: 3.949 ± 0.303
3.821GlyGlu: 3.821 ± 0.28
2.998GlyPhe: 2.998 ± 0.265
3.492GlyGly: 3.492 ± 0.277
1.207GlyHis: 1.207 ± 0.151
4.242GlyIle: 4.242 ± 0.278
4.461GlyLys: 4.461 ± 0.259
4.845GlyLeu: 4.845 ± 0.269
1.92GlyMet: 1.92 ± 0.201
3.309GlyAsn: 3.309 ± 0.371
1.883GlyPro: 1.883 ± 0.189
2.578GlyGln: 2.578 ± 0.261
2.486GlyArg: 2.486 ± 0.249
3.785GlySer: 3.785 ± 0.285
4.552GlyThr: 4.552 ± 0.402
3.949GlyVal: 3.949 ± 0.304
1.024GlyTrp: 1.024 ± 0.14
2.87GlyTyr: 2.87 ± 0.202
0.0GlyXaa: 0.0 ± 0.0
His
1.188HisAla: 1.188 ± 0.15
0.347HisCys: 0.347 ± 0.07
1.06HisAsp: 1.06 ± 0.139
1.353HisGlu: 1.353 ± 0.145
0.841HisPhe: 0.841 ± 0.126
1.152HisGly: 1.152 ± 0.132
0.585HisHis: 0.585 ± 0.121
1.426HisIle: 1.426 ± 0.158
1.499HisLys: 1.499 ± 0.163
1.737HisLeu: 1.737 ± 0.171
0.347HisMet: 0.347 ± 0.072
0.695HisAsn: 0.695 ± 0.106
0.951HisPro: 0.951 ± 0.135
0.622HisGln: 0.622 ± 0.091
0.804HisArg: 0.804 ± 0.138
1.188HisSer: 1.188 ± 0.143
0.841HisThr: 0.841 ± 0.129
1.298HisVal: 1.298 ± 0.149
0.311HisTrp: 0.311 ± 0.076
0.914HisTyr: 0.914 ± 0.122
0.0HisXaa: 0.0 ± 0.0
Ile
4.552IleAla: 4.552 ± 0.323
0.951IleCys: 0.951 ± 0.144
5.32IleAsp: 5.32 ± 0.324
5.521IleGlu: 5.521 ± 0.355
2.34IlePhe: 2.34 ± 0.187
3.913IleGly: 3.913 ± 0.326
1.28IleHis: 1.28 ± 0.163
4.022IleIle: 4.022 ± 0.285
5.96IleLys: 5.96 ± 0.312
4.552IleLeu: 4.552 ± 0.293
2.176IleMet: 2.176 ± 0.199
4.041IleAsn: 4.041 ± 0.276
2.578IlePro: 2.578 ± 0.236
2.45IleGln: 2.45 ± 0.185
3.291IleArg: 3.291 ± 0.24
4.406IleSer: 4.406 ± 0.261
5.01IleThr: 5.01 ± 0.337
4.534IleVal: 4.534 ± 0.297
0.64IleTrp: 0.64 ± 0.122
2.633IleTyr: 2.633 ± 0.251
0.0IleXaa: 0.0 ± 0.0
Lys
6.106LysAla: 6.106 ± 0.37
0.786LysCys: 0.786 ± 0.129
4.662LysAsp: 4.662 ± 0.329
5.723LysGlu: 5.723 ± 0.369
3.309LysPhe: 3.309 ± 0.257
4.041LysGly: 4.041 ± 0.242
1.463LysHis: 1.463 ± 0.162
4.662LysIle: 4.662 ± 0.297
4.351LysLys: 4.351 ± 0.372
6.344LysLeu: 6.344 ± 0.4
2.432LysMet: 2.432 ± 0.2
3.894LysAsn: 3.894 ± 0.243
2.56LysPro: 2.56 ± 0.213
2.688LysGln: 2.688 ± 0.25
3.346LysArg: 3.346 ± 0.256
4.296LysSer: 4.296 ± 0.288
4.406LysThr: 4.406 ± 0.267
4.882LysVal: 4.882 ± 0.277
1.17LysTrp: 1.17 ± 0.143
3.072LysTyr: 3.072 ± 0.21
0.0LysXaa: 0.0 ± 0.0
Leu
5.43LeuAla: 5.43 ± 0.357
0.786LeuCys: 0.786 ± 0.142
5.119LeuAsp: 5.119 ± 0.349
5.339LeuGlu: 5.339 ± 0.305
2.907LeuPhe: 2.907 ± 0.217
4.424LeuGly: 4.424 ± 0.289
1.371LeuHis: 1.371 ± 0.142
5.211LeuIle: 5.211 ± 0.274
6.326LeuLys: 6.326 ± 0.294
4.808LeuLeu: 4.808 ± 0.346
2.413LeuMet: 2.413 ± 0.254
4.242LeuAsn: 4.242 ± 0.286
3.017LeuPro: 3.017 ± 0.2
2.761LeuGln: 2.761 ± 0.226
3.748LeuArg: 3.748 ± 0.233
4.79LeuSer: 4.79 ± 0.341
4.498LeuThr: 4.498 ± 0.29
4.918LeuVal: 4.918 ± 0.265
0.987LeuTrp: 0.987 ± 0.142
3.163LeuTyr: 3.163 ± 0.24
0.0LeuXaa: 0.0 ± 0.0
Met
2.267MetAla: 2.267 ± 0.202
0.366MetCys: 0.366 ± 0.087
1.828MetAsp: 1.828 ± 0.161
1.499MetGlu: 1.499 ± 0.185
1.207MetPhe: 1.207 ± 0.17
1.28MetGly: 1.28 ± 0.153
0.603MetHis: 0.603 ± 0.102
2.249MetIle: 2.249 ± 0.197
2.779MetLys: 2.779 ± 0.235
2.267MetLeu: 2.267 ± 0.197
0.896MetMet: 0.896 ± 0.133
1.755MetAsn: 1.755 ± 0.179
0.878MetPro: 0.878 ± 0.121
1.316MetGln: 1.316 ± 0.172
1.042MetArg: 1.042 ± 0.142
2.267MetSer: 2.267 ± 0.205
2.029MetThr: 2.029 ± 0.218
1.517MetVal: 1.517 ± 0.153
0.238MetTrp: 0.238 ± 0.064
1.097MetTyr: 1.097 ± 0.123
0.0MetXaa: 0.0 ± 0.0
Asn
3.327AsnAla: 3.327 ± 0.247
0.512AsnCys: 0.512 ± 0.092
3.291AsnAsp: 3.291 ± 0.248
4.004AsnGlu: 4.004 ± 0.23
2.395AsnPhe: 2.395 ± 0.205
3.839AsnGly: 3.839 ± 0.383
1.079AsnHis: 1.079 ± 0.12
3.455AsnIle: 3.455 ± 0.23
3.748AsnLys: 3.748 ± 0.247
4.077AsnLeu: 4.077 ± 0.246
1.627AsnMet: 1.627 ± 0.173
2.578AsnAsn: 2.578 ± 0.231
2.029AsnPro: 2.029 ± 0.203
1.81AsnGln: 1.81 ± 0.191
2.358AsnArg: 2.358 ± 0.177
2.688AsnSer: 2.688 ± 0.192
2.56AsnThr: 2.56 ± 0.22
3.492AsnVal: 3.492 ± 0.308
0.658AsnTrp: 0.658 ± 0.108
1.92AsnTyr: 1.92 ± 0.21
0.0AsnXaa: 0.0 ± 0.0
Pro
2.34ProAla: 2.34 ± 0.22
0.421ProCys: 0.421 ± 0.085
2.486ProAsp: 2.486 ± 0.214
2.724ProGlu: 2.724 ± 0.235
1.719ProPhe: 1.719 ± 0.174
2.103ProGly: 2.103 ± 0.2
0.64ProHis: 0.64 ± 0.096
2.596ProIle: 2.596 ± 0.216
2.231ProLys: 2.231 ± 0.19
2.432ProLeu: 2.432 ± 0.186
0.823ProMet: 0.823 ± 0.103
2.212ProAsn: 2.212 ± 0.184
0.914ProPro: 0.914 ± 0.174
0.951ProGln: 0.951 ± 0.115
1.426ProArg: 1.426 ± 0.165
2.34ProSer: 2.34 ± 0.228
2.212ProThr: 2.212 ± 0.202
2.377ProVal: 2.377 ± 0.236
0.658ProTrp: 0.658 ± 0.124
1.28ProTyr: 1.28 ± 0.149
0.0ProXaa: 0.0 ± 0.0
Gln
2.486GlnAla: 2.486 ± 0.198
0.238GlnCys: 0.238 ± 0.067
1.847GlnAsp: 1.847 ± 0.206
2.413GlnGlu: 2.413 ± 0.202
1.645GlnPhe: 1.645 ± 0.161
1.92GlnGly: 1.92 ± 0.184
0.841GlnHis: 0.841 ± 0.136
2.56GlnIle: 2.56 ± 0.222
2.377GlnLys: 2.377 ± 0.196
2.816GlnLeu: 2.816 ± 0.184
0.932GlnMet: 0.932 ± 0.134
1.298GlnAsn: 1.298 ± 0.147
1.188GlnPro: 1.188 ± 0.173
1.006GlnGln: 1.006 ± 0.144
1.664GlnArg: 1.664 ± 0.187
1.883GlnSer: 1.883 ± 0.169
2.066GlnThr: 2.066 ± 0.182
2.523GlnVal: 2.523 ± 0.208
0.823GlnTrp: 0.823 ± 0.115
1.847GlnTyr: 1.847 ± 0.176
0.0GlnXaa: 0.0 ± 0.0
Arg
2.724ArgAla: 2.724 ± 0.228
0.567ArgCys: 0.567 ± 0.099
2.907ArgAsp: 2.907 ± 0.243
3.145ArgGlu: 3.145 ± 0.253
1.956ArgPhe: 1.956 ± 0.193
2.87ArgGly: 2.87 ± 0.272
0.786ArgHis: 0.786 ± 0.122
3.675ArgIle: 3.675 ± 0.295
3.236ArgLys: 3.236 ± 0.257
3.126ArgLeu: 3.126 ± 0.249
1.389ArgMet: 1.389 ± 0.169
2.029ArgAsn: 2.029 ± 0.17
1.134ArgPro: 1.134 ± 0.146
1.682ArgGln: 1.682 ± 0.17
2.066ArgArg: 2.066 ± 0.195
2.633ArgSer: 2.633 ± 0.249
2.56ArgThr: 2.56 ± 0.246
2.761ArgVal: 2.761 ± 0.219
0.823ArgTrp: 0.823 ± 0.136
1.847ArgTyr: 1.847 ± 0.183
0.0ArgXaa: 0.0 ± 0.0
Ser
3.949SerAla: 3.949 ± 0.294
0.786SerCys: 0.786 ± 0.133
3.949SerAsp: 3.949 ± 0.282
4.223SerGlu: 4.223 ± 0.281
2.907SerPhe: 2.907 ± 0.254
4.26SerGly: 4.26 ± 0.276
1.042SerHis: 1.042 ± 0.124
4.278SerIle: 4.278 ± 0.247
4.772SerLys: 4.772 ± 0.32
4.918SerLeu: 4.918 ± 0.261
1.938SerMet: 1.938 ± 0.2
2.852SerAsn: 2.852 ± 0.201
2.34SerPro: 2.34 ± 0.222
2.029SerGln: 2.029 ± 0.182
2.998SerArg: 2.998 ± 0.212
4.114SerSer: 4.114 ± 0.25
3.638SerThr: 3.638 ± 0.272
4.041SerVal: 4.041 ± 0.275
0.823SerTrp: 0.823 ± 0.13
2.633SerTyr: 2.633 ± 0.227
0.0SerXaa: 0.0 ± 0.0
Thr
4.223ThrAla: 4.223 ± 0.358
0.585ThrCys: 0.585 ± 0.091
3.492ThrAsp: 3.492 ± 0.318
3.839ThrGlu: 3.839 ± 0.278
2.816ThrPhe: 2.816 ± 0.236
4.424ThrGly: 4.424 ± 0.316
1.024ThrHis: 1.024 ± 0.13
4.351ThrIle: 4.351 ± 0.336
4.498ThrLys: 4.498 ± 0.307
4.315ThrLeu: 4.315 ± 0.311
1.389ThrMet: 1.389 ± 0.174
2.834ThrAsn: 2.834 ± 0.233
2.889ThrPro: 2.889 ± 0.287
2.194ThrGln: 2.194 ± 0.222
2.432ThrArg: 2.432 ± 0.193
3.967ThrSer: 3.967 ± 0.361
3.967ThrThr: 3.967 ± 0.316
4.534ThrVal: 4.534 ± 0.328
0.841ThrTrp: 0.841 ± 0.134
2.194ThrTyr: 2.194 ± 0.241
0.0ThrXaa: 0.0 ± 0.0
Val
4.717ValAla: 4.717 ± 0.31
0.841ValCys: 0.841 ± 0.131
4.205ValAsp: 4.205 ± 0.315
5.028ValGlu: 5.028 ± 0.315
2.724ValPhe: 2.724 ± 0.244
3.675ValGly: 3.675 ± 0.29
1.079ValHis: 1.079 ± 0.141
5.028ValIle: 5.028 ± 0.266
5.375ValLys: 5.375 ± 0.318
4.772ValLeu: 4.772 ± 0.284
1.865ValMet: 1.865 ± 0.173
3.291ValAsn: 3.291 ± 0.244
2.066ValPro: 2.066 ± 0.192
2.249ValGln: 2.249 ± 0.2
3.035ValArg: 3.035 ± 0.243
4.424ValSer: 4.424 ± 0.297
3.602ValThr: 3.602 ± 0.306
4.644ValVal: 4.644 ± 0.298
1.024ValTrp: 1.024 ± 0.117
2.688ValTyr: 2.688 ± 0.213
0.0ValXaa: 0.0 ± 0.0
Trp
1.006TrpAla: 1.006 ± 0.153
0.201TrpCys: 0.201 ± 0.061
1.152TrpAsp: 1.152 ± 0.137
0.932TrpGlu: 0.932 ± 0.12
0.804TrpPhe: 0.804 ± 0.106
0.64TrpGly: 0.64 ± 0.138
0.293TrpHis: 0.293 ± 0.077
0.75TrpIle: 0.75 ± 0.124
1.152TrpLys: 1.152 ± 0.131
1.188TrpLeu: 1.188 ± 0.14
0.585TrpMet: 0.585 ± 0.116
0.603TrpAsn: 0.603 ± 0.099
0.512TrpPro: 0.512 ± 0.094
0.475TrpGln: 0.475 ± 0.095
0.695TrpArg: 0.695 ± 0.11
0.951TrpSer: 0.951 ± 0.132
1.024TrpThr: 1.024 ± 0.137
1.079TrpVal: 1.079 ± 0.164
0.274TrpTrp: 0.274 ± 0.081
0.75TrpTyr: 0.75 ± 0.124
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.834TyrAla: 2.834 ± 0.226
0.567TyrCys: 0.567 ± 0.11
3.09TyrAsp: 3.09 ± 0.261
2.596TyrGlu: 2.596 ± 0.232
1.664TyrPhe: 1.664 ± 0.166
2.523TyrGly: 2.523 ± 0.231
0.932TyrHis: 0.932 ± 0.132
2.688TyrIle: 2.688 ± 0.236
2.779TyrLys: 2.779 ± 0.233
3.053TyrLeu: 3.053 ± 0.294
0.75TyrMet: 0.75 ± 0.14
2.596TyrAsn: 2.596 ± 0.238
1.536TyrPro: 1.536 ± 0.185
1.499TyrGln: 1.499 ± 0.18
1.828TyrArg: 1.828 ± 0.162
2.706TyrSer: 2.706 ± 0.267
2.267TyrThr: 2.267 ± 0.265
2.797TyrVal: 2.797 ± 0.217
0.585TyrTrp: 0.585 ± 0.085
1.536TyrTyr: 1.536 ± 0.184
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 295 proteins (54697 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski