Mycobacterium phage Steamy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345L0P7|A0A345L0P7_9CAUD Membrane protein OS=Mycobacterium phage Steamy OX=2250309 GN=75 PE=4 SV=1
MM1 pKa = 7.03KK2 pKa = 9.94TITFYY7 pKa = 9.73VHH9 pKa = 6.96RR10 pKa = 11.84SSSYY14 pKa = 8.24TYY16 pKa = 7.89EE17 pKa = 4.08TEE19 pKa = 3.99VPEE22 pKa = 5.79DD23 pKa = 4.0FDD25 pKa = 4.41TSDD28 pKa = 3.2EE29 pKa = 4.25TALLDD34 pKa = 5.01LADD37 pKa = 4.68GLDD40 pKa = 3.87PVDD43 pKa = 5.85DD44 pKa = 4.23EE45 pKa = 4.98TNHH48 pKa = 6.23LSAFII53 pKa = 4.45

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345L0J6|A0A345L0J6_9CAUD Tail terminator OS=Mycobacterium phage Steamy OX=2250309 GN=23 PE=4 SV=1
MM1 pKa = 7.23ARR3 pKa = 11.84VYY5 pKa = 11.48ANANSAAAHH14 pKa = 5.86HH15 pKa = 6.75VDD17 pKa = 2.88TRR19 pKa = 11.84RR20 pKa = 11.84AIRR23 pKa = 11.84KK24 pKa = 7.07EE25 pKa = 3.69AQEE28 pKa = 3.92GGRR31 pKa = 11.84RR32 pKa = 11.84SEE34 pKa = 4.24ARR36 pKa = 11.84LASARR41 pKa = 11.84ASSDD45 pKa = 2.74HH46 pKa = 5.67TKK48 pKa = 10.16IYY50 pKa = 10.54GPSHH54 pKa = 4.22QTKK57 pKa = 7.91VTVTHH62 pKa = 6.56GVLDD66 pKa = 3.62SFINLEE72 pKa = 4.16GTDD75 pKa = 3.46PMAIEE80 pKa = 5.05FGHH83 pKa = 6.26FPSGYY88 pKa = 9.1FAPEE92 pKa = 3.41RR93 pKa = 11.84FGRR96 pKa = 11.84MTKK99 pKa = 10.15SPSGLYY105 pKa = 10.1IITLGSGAGGSMAVSSGRR123 pKa = 11.84KK124 pKa = 7.97RR125 pKa = 11.84GKK127 pKa = 9.93RR128 pKa = 3.3

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

16734

37

1004

185.9

20.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.332 ± 0.37

0.825 ± 0.12

5.964 ± 0.184

6.424 ± 0.344

3.341 ± 0.167

8.695 ± 0.53

1.87 ± 0.179

4.912 ± 0.163

4.625 ± 0.199

7.787 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.504 ± 0.121

3.37 ± 0.175

5.462 ± 0.27

3.58 ± 0.217

6.233 ± 0.331

5.898 ± 0.234

6.275 ± 0.214

7.052 ± 0.209

1.984 ± 0.134

2.868 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski