Amino acid dipepetide frequency for Escherichia phage vB_Eco_SLUR29

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.224AlaAla: 7.224 ± 0.959
0.613AlaCys: 0.613 ± 0.207
3.884AlaAsp: 3.884 ± 0.566
5.384AlaGlu: 5.384 ± 0.665
3.067AlaPhe: 3.067 ± 0.541
5.861AlaGly: 5.861 ± 0.635
0.886AlaHis: 0.886 ± 0.239
6.27AlaIle: 6.27 ± 0.729
6.201AlaLys: 6.201 ± 0.69
5.861AlaLeu: 5.861 ± 0.64
2.862AlaMet: 2.862 ± 0.428
4.225AlaAsn: 4.225 ± 0.664
1.772AlaPro: 1.772 ± 0.345
3.135AlaGln: 3.135 ± 0.408
5.111AlaArg: 5.111 ± 0.619
5.384AlaSer: 5.384 ± 0.485
3.544AlaThr: 3.544 ± 0.453
4.907AlaVal: 4.907 ± 0.588
0.954AlaTrp: 0.954 ± 0.236
2.862AlaTyr: 2.862 ± 0.564
0.0AlaXaa: 0.0 ± 0.0
Cys
1.022CysAla: 1.022 ± 0.261
0.273CysCys: 0.273 ± 0.131
1.499CysAsp: 1.499 ± 0.35
0.886CysGlu: 0.886 ± 0.248
0.341CysPhe: 0.341 ± 0.142
1.499CysGly: 1.499 ± 0.331
0.204CysHis: 0.204 ± 0.132
0.477CysIle: 0.477 ± 0.171
0.75CysLys: 0.75 ± 0.314
1.022CysLeu: 1.022 ± 0.277
0.545CysMet: 0.545 ± 0.165
0.477CysAsn: 0.477 ± 0.158
0.409CysPro: 0.409 ± 0.151
0.204CysGln: 0.204 ± 0.134
1.022CysArg: 1.022 ± 0.304
0.681CysSer: 0.681 ± 0.176
0.545CysThr: 0.545 ± 0.228
0.818CysVal: 0.818 ± 0.232
0.409CysTrp: 0.409 ± 0.157
0.681CysTyr: 0.681 ± 0.201
0.0CysXaa: 0.0 ± 0.0
Asp
4.975AspAla: 4.975 ± 0.547
0.954AspCys: 0.954 ± 0.313
4.225AspAsp: 4.225 ± 0.641
4.498AspGlu: 4.498 ± 0.49
2.658AspPhe: 2.658 ± 0.393
7.156AspGly: 7.156 ± 0.846
0.75AspHis: 0.75 ± 0.192
3.203AspIle: 3.203 ± 0.381
4.566AspLys: 4.566 ± 0.495
4.43AspLeu: 4.43 ± 0.517
1.976AspMet: 1.976 ± 0.315
2.317AspAsn: 2.317 ± 0.34
2.453AspPro: 2.453 ± 0.372
1.908AspGln: 1.908 ± 0.36
2.794AspArg: 2.794 ± 0.397
3.816AspSer: 3.816 ± 0.473
2.999AspThr: 2.999 ± 0.39
5.52AspVal: 5.52 ± 0.707
1.295AspTrp: 1.295 ± 0.322
2.317AspTyr: 2.317 ± 0.364
0.0AspXaa: 0.0 ± 0.0
Glu
5.52GluAla: 5.52 ± 0.737
0.954GluCys: 0.954 ± 0.216
3.544GluAsp: 3.544 ± 0.403
4.634GluGlu: 4.634 ± 0.681
3.067GluPhe: 3.067 ± 0.537
3.271GluGly: 3.271 ± 0.422
1.227GluHis: 1.227 ± 0.303
5.452GluIle: 5.452 ± 0.547
4.43GluLys: 4.43 ± 0.561
4.566GluLeu: 4.566 ± 0.768
2.658GluMet: 2.658 ± 0.38
3.816GluAsn: 3.816 ± 0.41
1.84GluPro: 1.84 ± 0.322
2.93GluGln: 2.93 ± 0.6
3.476GluArg: 3.476 ± 0.518
4.361GluSer: 4.361 ± 0.45
3.68GluThr: 3.68 ± 0.374
4.293GluVal: 4.293 ± 0.538
0.886GluTrp: 0.886 ± 0.225
3.407GluTyr: 3.407 ± 0.447
0.0GluXaa: 0.0 ± 0.0
Phe
2.726PheAla: 2.726 ± 0.44
0.613PheCys: 0.613 ± 0.212
3.271PheAsp: 3.271 ± 0.525
2.044PheGlu: 2.044 ± 0.324
1.09PhePhe: 1.09 ± 0.247
3.748PheGly: 3.748 ± 0.486
0.477PheHis: 0.477 ± 0.158
2.317PheIle: 2.317 ± 0.367
3.203PheLys: 3.203 ± 0.43
2.658PheLeu: 2.658 ± 0.536
0.818PheMet: 0.818 ± 0.259
2.113PheAsn: 2.113 ± 0.357
1.227PhePro: 1.227 ± 0.294
1.772PheGln: 1.772 ± 0.346
1.976PheArg: 1.976 ± 0.296
1.976PheSer: 1.976 ± 0.365
2.862PheThr: 2.862 ± 0.425
2.59PheVal: 2.59 ± 0.419
0.818PheTrp: 0.818 ± 0.248
1.363PheTyr: 1.363 ± 0.33
0.0PheXaa: 0.0 ± 0.0
Gly
4.907GlyAla: 4.907 ± 0.713
1.431GlyCys: 1.431 ± 0.365
5.452GlyAsp: 5.452 ± 0.596
4.566GlyGlu: 4.566 ± 0.56
2.999GlyPhe: 2.999 ± 0.404
6.27GlyGly: 6.27 ± 1.089
1.295GlyHis: 1.295 ± 0.322
4.498GlyIle: 4.498 ± 0.507
6.338GlyLys: 6.338 ± 0.568
5.043GlyLeu: 5.043 ± 0.485
2.453GlyMet: 2.453 ± 0.379
3.612GlyAsn: 3.612 ± 0.397
0.409GlyPro: 0.409 ± 0.153
2.453GlyGln: 2.453 ± 0.469
3.203GlyArg: 3.203 ± 0.428
5.247GlySer: 5.247 ± 0.54
2.726GlyThr: 2.726 ± 0.517
5.384GlyVal: 5.384 ± 0.577
1.431GlyTrp: 1.431 ± 0.267
3.544GlyTyr: 3.544 ± 0.511
0.0GlyXaa: 0.0 ± 0.0
His
0.75HisAla: 0.75 ± 0.196
0.204HisCys: 0.204 ± 0.094
1.09HisAsp: 1.09 ± 0.309
1.159HisGlu: 1.159 ± 0.244
0.613HisPhe: 0.613 ± 0.185
1.295HisGly: 1.295 ± 0.282
0.613HisHis: 0.613 ± 0.248
0.75HisIle: 0.75 ± 0.24
1.159HisLys: 1.159 ± 0.337
1.09HisLeu: 1.09 ± 0.284
0.341HisMet: 0.341 ± 0.178
0.613HisAsn: 0.613 ± 0.196
0.886HisPro: 0.886 ± 0.281
0.613HisGln: 0.613 ± 0.218
1.227HisArg: 1.227 ± 0.287
0.681HisSer: 0.681 ± 0.194
0.477HisThr: 0.477 ± 0.205
1.704HisVal: 1.704 ± 0.325
0.204HisTrp: 0.204 ± 0.11
1.022HisTyr: 1.022 ± 0.264
0.0HisXaa: 0.0 ± 0.0
Ile
6.201IleAla: 6.201 ± 0.549
1.09IleCys: 1.09 ± 0.315
5.384IleAsp: 5.384 ± 0.688
4.361IleGlu: 4.361 ± 0.496
1.908IlePhe: 1.908 ± 0.296
3.816IleGly: 3.816 ± 0.454
1.159IleHis: 1.159 ± 0.39
3.953IleIle: 3.953 ± 0.442
5.043IleLys: 5.043 ± 0.756
3.271IleLeu: 3.271 ± 0.407
1.976IleMet: 1.976 ± 0.403
3.884IleAsn: 3.884 ± 0.536
2.317IlePro: 2.317 ± 0.406
2.249IleGln: 2.249 ± 0.401
3.407IleArg: 3.407 ± 0.516
4.498IleSer: 4.498 ± 0.693
4.566IleThr: 4.566 ± 0.67
4.225IleVal: 4.225 ± 0.469
1.09IleTrp: 1.09 ± 0.258
2.113IleTyr: 2.113 ± 0.371
0.0IleXaa: 0.0 ± 0.0
Lys
6.201LysAla: 6.201 ± 0.965
1.227LysCys: 1.227 ± 0.288
4.838LysAsp: 4.838 ± 0.523
6.474LysGlu: 6.474 ± 0.638
3.067LysPhe: 3.067 ± 0.39
4.361LysGly: 4.361 ± 0.609
1.022LysHis: 1.022 ± 0.289
4.566LysIle: 4.566 ± 0.644
5.588LysLys: 5.588 ± 0.872
5.384LysLeu: 5.384 ± 0.598
2.999LysMet: 2.999 ± 0.496
2.93LysAsn: 2.93 ± 0.509
3.407LysPro: 3.407 ± 0.489
3.612LysGln: 3.612 ± 0.548
4.43LysArg: 4.43 ± 0.592
3.339LysSer: 3.339 ± 0.468
4.702LysThr: 4.702 ± 0.458
4.361LysVal: 4.361 ± 0.549
1.022LysTrp: 1.022 ± 0.264
2.181LysTyr: 2.181 ± 0.348
0.0LysXaa: 0.0 ± 0.0
Leu
5.452LeuAla: 5.452 ± 0.608
0.681LeuCys: 0.681 ± 0.269
4.77LeuAsp: 4.77 ± 0.625
3.953LeuGlu: 3.953 ± 0.641
2.044LeuPhe: 2.044 ± 0.433
3.953LeuGly: 3.953 ± 0.746
1.022LeuHis: 1.022 ± 0.248
4.293LeuIle: 4.293 ± 0.54
5.247LeuLys: 5.247 ± 0.799
4.089LeuLeu: 4.089 ± 0.543
2.249LeuMet: 2.249 ± 0.443
3.067LeuAsn: 3.067 ± 0.518
3.067LeuPro: 3.067 ± 0.519
2.044LeuGln: 2.044 ± 0.278
3.544LeuArg: 3.544 ± 0.505
3.407LeuSer: 3.407 ± 0.37
4.43LeuThr: 4.43 ± 0.561
4.634LeuVal: 4.634 ± 0.445
0.75LeuTrp: 0.75 ± 0.233
2.999LeuTyr: 2.999 ± 0.4
0.0LeuXaa: 0.0 ± 0.0
Met
4.021MetAla: 4.021 ± 0.485
0.341MetCys: 0.341 ± 0.16
1.431MetAsp: 1.431 ± 0.274
1.431MetGlu: 1.431 ± 0.389
1.227MetPhe: 1.227 ± 0.292
1.499MetGly: 1.499 ± 0.313
0.477MetHis: 0.477 ± 0.171
3.203MetIle: 3.203 ± 0.413
2.726MetLys: 2.726 ± 0.385
1.976MetLeu: 1.976 ± 0.388
0.886MetMet: 0.886 ± 0.241
1.363MetAsn: 1.363 ± 0.308
0.75MetPro: 0.75 ± 0.193
1.09MetGln: 1.09 ± 0.268
1.908MetArg: 1.908 ± 0.366
1.636MetSer: 1.636 ± 0.368
1.908MetThr: 1.908 ± 0.307
1.567MetVal: 1.567 ± 0.265
0.204MetTrp: 0.204 ± 0.123
0.886MetTyr: 0.886 ± 0.202
0.0MetXaa: 0.0 ± 0.0
Asn
4.157AsnAla: 4.157 ± 0.542
0.341AsnCys: 0.341 ± 0.161
2.999AsnAsp: 2.999 ± 0.487
3.544AsnGlu: 3.544 ± 0.42
1.704AsnPhe: 1.704 ± 0.269
5.111AsnGly: 5.111 ± 0.546
1.159AsnHis: 1.159 ± 0.268
3.407AsnIle: 3.407 ± 0.503
3.884AsnLys: 3.884 ± 0.47
2.453AsnLeu: 2.453 ± 0.403
1.227AsnMet: 1.227 ± 0.296
2.181AsnAsn: 2.181 ± 0.348
1.431AsnPro: 1.431 ± 0.304
1.363AsnGln: 1.363 ± 0.309
1.567AsnArg: 1.567 ± 0.329
2.93AsnSer: 2.93 ± 0.507
1.431AsnThr: 1.431 ± 0.307
3.407AsnVal: 3.407 ± 0.388
0.886AsnTrp: 0.886 ± 0.203
1.499AsnTyr: 1.499 ± 0.359
0.0AsnXaa: 0.0 ± 0.0
Pro
2.044ProAla: 2.044 ± 0.291
0.477ProCys: 0.477 ± 0.229
2.726ProAsp: 2.726 ± 0.474
2.93ProGlu: 2.93 ± 0.52
1.431ProPhe: 1.431 ± 0.245
2.658ProGly: 2.658 ± 0.377
0.409ProHis: 0.409 ± 0.157
2.181ProIle: 2.181 ± 0.383
1.295ProLys: 1.295 ± 0.224
2.044ProLeu: 2.044 ± 0.437
0.75ProMet: 0.75 ± 0.197
1.431ProAsn: 1.431 ± 0.308
1.227ProPro: 1.227 ± 0.28
1.159ProGln: 1.159 ± 0.268
1.567ProArg: 1.567 ± 0.347
2.044ProSer: 2.044 ± 0.367
1.704ProThr: 1.704 ± 0.293
2.999ProVal: 2.999 ± 0.366
0.273ProTrp: 0.273 ± 0.111
1.704ProTyr: 1.704 ± 0.282
0.0ProXaa: 0.0 ± 0.0
Gln
2.726GlnAla: 2.726 ± 0.489
0.477GlnCys: 0.477 ± 0.172
2.521GlnAsp: 2.521 ± 0.346
2.999GlnGlu: 2.999 ± 0.564
1.499GlnPhe: 1.499 ± 0.299
2.249GlnGly: 2.249 ± 0.381
0.75GlnHis: 0.75 ± 0.259
2.249GlnIle: 2.249 ± 0.561
2.385GlnLys: 2.385 ± 0.426
2.726GlnLeu: 2.726 ± 0.433
0.954GlnMet: 0.954 ± 0.222
1.09GlnAsn: 1.09 ± 0.269
1.159GlnPro: 1.159 ± 0.362
3.135GlnGln: 3.135 ± 0.882
1.84GlnArg: 1.84 ± 0.28
2.113GlnSer: 2.113 ± 0.376
1.567GlnThr: 1.567 ± 0.304
3.203GlnVal: 3.203 ± 0.463
0.75GlnTrp: 0.75 ± 0.261
1.772GlnTyr: 1.772 ± 0.328
0.0GlnXaa: 0.0 ± 0.0
Arg
4.43ArgAla: 4.43 ± 0.623
0.75ArgCys: 0.75 ± 0.295
3.407ArgAsp: 3.407 ± 0.508
3.68ArgGlu: 3.68 ± 0.444
2.658ArgPhe: 2.658 ± 0.42
2.59ArgGly: 2.59 ± 0.425
0.886ArgHis: 0.886 ± 0.247
3.68ArgIle: 3.68 ± 0.39
4.907ArgLys: 4.907 ± 0.593
3.203ArgLeu: 3.203 ± 0.599
1.499ArgMet: 1.499 ± 0.304
1.84ArgAsn: 1.84 ± 0.49
1.704ArgPro: 1.704 ± 0.377
1.567ArgGln: 1.567 ± 0.363
3.407ArgArg: 3.407 ± 0.425
3.203ArgSer: 3.203 ± 0.444
1.636ArgThr: 1.636 ± 0.338
4.566ArgVal: 4.566 ± 0.615
0.75ArgTrp: 0.75 ± 0.241
2.249ArgTyr: 2.249 ± 0.365
0.0ArgXaa: 0.0 ± 0.0
Ser
4.702SerAla: 4.702 ± 0.61
0.613SerCys: 0.613 ± 0.2
3.68SerAsp: 3.68 ± 0.425
4.157SerGlu: 4.157 ± 0.503
2.658SerPhe: 2.658 ± 0.392
4.838SerGly: 4.838 ± 0.486
0.75SerHis: 0.75 ± 0.217
4.566SerIle: 4.566 ± 0.62
4.089SerLys: 4.089 ± 0.506
4.634SerLeu: 4.634 ± 0.445
1.022SerMet: 1.022 ± 0.296
2.999SerAsn: 2.999 ± 0.371
1.976SerPro: 1.976 ± 0.34
2.453SerGln: 2.453 ± 0.36
2.93SerArg: 2.93 ± 0.382
3.407SerSer: 3.407 ± 0.511
3.544SerThr: 3.544 ± 0.494
3.953SerVal: 3.953 ± 0.454
0.954SerTrp: 0.954 ± 0.261
2.181SerTyr: 2.181 ± 0.458
0.0SerXaa: 0.0 ± 0.0
Thr
4.566ThrAla: 4.566 ± 0.689
0.545ThrCys: 0.545 ± 0.212
2.658ThrAsp: 2.658 ± 0.401
2.794ThrGlu: 2.794 ± 0.411
2.317ThrPhe: 2.317 ± 0.338
5.997ThrGly: 5.997 ± 0.901
0.409ThrHis: 0.409 ± 0.153
3.544ThrIle: 3.544 ± 0.562
3.135ThrLys: 3.135 ± 0.531
3.544ThrLeu: 3.544 ± 0.441
1.431ThrMet: 1.431 ± 0.286
3.135ThrAsn: 3.135 ± 0.513
2.181ThrPro: 2.181 ± 0.394
1.976ThrGln: 1.976 ± 0.504
1.772ThrArg: 1.772 ± 0.295
2.59ThrSer: 2.59 ± 0.465
2.385ThrThr: 2.385 ± 0.399
3.953ThrVal: 3.953 ± 0.549
0.613ThrTrp: 0.613 ± 0.192
2.113ThrTyr: 2.113 ± 0.415
0.0ThrXaa: 0.0 ± 0.0
Val
4.702ValAla: 4.702 ± 0.753
1.159ValCys: 1.159 ± 0.239
4.089ValAsp: 4.089 ± 0.429
5.111ValGlu: 5.111 ± 0.648
3.407ValPhe: 3.407 ± 0.519
3.407ValGly: 3.407 ± 0.528
1.295ValHis: 1.295 ± 0.338
4.838ValIle: 4.838 ± 0.661
6.61ValLys: 6.61 ± 0.578
4.089ValLeu: 4.089 ± 0.424
2.249ValMet: 2.249 ± 0.257
3.203ValAsn: 3.203 ± 0.474
2.658ValPro: 2.658 ± 0.573
2.385ValGln: 2.385 ± 0.41
3.407ValArg: 3.407 ± 0.433
5.384ValSer: 5.384 ± 0.577
3.884ValThr: 3.884 ± 0.537
4.77ValVal: 4.77 ± 0.617
1.159ValTrp: 1.159 ± 0.27
1.84ValTyr: 1.84 ± 0.371
0.0ValXaa: 0.0 ± 0.0
Trp
0.818TrpAla: 0.818 ± 0.267
0.341TrpCys: 0.341 ± 0.127
0.75TrpAsp: 0.75 ± 0.194
0.75TrpGlu: 0.75 ± 0.228
0.681TrpPhe: 0.681 ± 0.254
1.09TrpGly: 1.09 ± 0.253
0.818TrpHis: 0.818 ± 0.215
0.886TrpIle: 0.886 ± 0.237
0.954TrpLys: 0.954 ± 0.252
1.567TrpLeu: 1.567 ± 0.315
0.409TrpMet: 0.409 ± 0.148
0.681TrpAsn: 0.681 ± 0.203
0.477TrpPro: 0.477 ± 0.193
0.204TrpGln: 0.204 ± 0.114
1.227TrpArg: 1.227 ± 0.264
0.954TrpSer: 0.954 ± 0.228
0.886TrpThr: 0.886 ± 0.212
0.681TrpVal: 0.681 ± 0.201
0.204TrpTrp: 0.204 ± 0.115
0.681TrpTyr: 0.681 ± 0.2
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.862TyrAla: 2.862 ± 0.415
0.681TyrCys: 0.681 ± 0.212
2.59TyrAsp: 2.59 ± 0.359
2.658TyrGlu: 2.658 ± 0.469
1.227TyrPhe: 1.227 ± 0.285
2.59TyrGly: 2.59 ± 0.445
0.886TyrHis: 0.886 ± 0.229
2.181TyrIle: 2.181 ± 0.341
3.203TyrLys: 3.203 ± 0.443
2.044TyrLeu: 2.044 ± 0.363
1.159TyrMet: 1.159 ± 0.217
1.704TyrAsn: 1.704 ± 0.352
1.704TyrPro: 1.704 ± 0.346
1.908TyrGln: 1.908 ± 0.287
2.794TyrArg: 2.794 ± 0.448
2.521TyrSer: 2.521 ± 0.369
2.181TyrThr: 2.181 ± 0.29
2.113TyrVal: 2.113 ± 0.372
0.409TyrTrp: 0.409 ± 0.139
1.159TyrTyr: 1.159 ± 0.256
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (14675 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski