Pyrus ussuriensis x Pyrus communis

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Pyrus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41937 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N5GZS8|A0A5N5GZS8_9ROSA Protein ROOT INITIATION DEFECTIVE 3 OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_015270 PE=4 SV=1
MM1 pKa = 7.33MLRR4 pKa = 11.84MNNEE8 pKa = 3.52GSDD11 pKa = 3.68YY12 pKa = 11.04GSWEE16 pKa = 3.86AGSYY20 pKa = 7.05VHH22 pKa = 7.41EE23 pKa = 6.0DD24 pKa = 3.13DD25 pKa = 6.31DD26 pKa = 5.76DD27 pKa = 4.99FNEE30 pKa = 4.57FDD32 pKa = 6.1DD33 pKa = 6.07DD34 pKa = 5.94ADD36 pKa = 4.76DD37 pKa = 4.67NDD39 pKa = 4.34DD40 pKa = 3.56EE41 pKa = 4.21EE42 pKa = 6.83RR43 pKa = 11.84YY44 pKa = 10.74DD45 pKa = 3.67GTEE48 pKa = 3.48VDD50 pKa = 3.6NDD52 pKa = 2.81EE53 pKa = 5.54DD54 pKa = 4.05AFNLHH59 pKa = 6.2AHH61 pKa = 7.23DD62 pKa = 6.15DD63 pKa = 3.82AGEE66 pKa = 4.37DD67 pKa = 3.56DD68 pKa = 4.44NPIVEE73 pKa = 4.42YY74 pKa = 10.75GPAVFSSDD82 pKa = 2.92EE83 pKa = 4.06AYY85 pKa = 10.58ARR87 pKa = 11.84ALQDD91 pKa = 3.52AEE93 pKa = 4.24DD94 pKa = 4.41RR95 pKa = 11.84EE96 pKa = 4.39MAARR100 pKa = 11.84LMALSGINDD109 pKa = 3.71RR110 pKa = 11.84EE111 pKa = 4.21VEE113 pKa = 4.12DD114 pKa = 4.23TEE116 pKa = 4.24EE117 pKa = 4.51HH118 pKa = 6.46GGNSQDD124 pKa = 2.96TWDD127 pKa = 4.91EE128 pKa = 3.78IDD130 pKa = 4.25PDD132 pKa = 3.74EE133 pKa = 5.11LSYY136 pKa = 11.75EE137 pKa = 4.12EE138 pKa = 5.6LLALSDD144 pKa = 3.95VVGTEE149 pKa = 4.06SRR151 pKa = 11.84GLSADD156 pKa = 4.07NIASLPSVSYY166 pKa = 8.71KK167 pKa = 10.3TGSSQNGSNEE177 pKa = 3.84SCVICRR183 pKa = 11.84LDD185 pKa = 3.72YY186 pKa = 11.4EE187 pKa = 4.79DD188 pKa = 5.88GEE190 pKa = 4.46NLTLLSCKK198 pKa = 9.98HH199 pKa = 6.5SYY201 pKa = 10.37HH202 pKa = 6.98SEE204 pKa = 4.38CINNWLKK211 pKa = 10.63INKK214 pKa = 8.01VCPVCSAEE222 pKa = 4.32VSTSANKK229 pKa = 10.08

Molecular weight: 25.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N5G112|A0A5N5G112_9ROSA Receptor-like serine/threonine-protein kinase OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_007077 PE=3 SV=1
MM1 pKa = 7.53SVGLHH6 pKa = 5.13PTAQNTARR14 pKa = 11.84VLLINANLPLTASLPQQHH32 pKa = 5.69NTHH35 pKa = 6.89FSPAGSHH42 pKa = 6.05RR43 pKa = 11.84RR44 pKa = 11.84TSNKK48 pKa = 8.02EE49 pKa = 3.67RR50 pKa = 11.84KK51 pKa = 9.02GLQSRR56 pKa = 11.84FAAMRR61 pKa = 11.84AKK63 pKa = 9.19WKK65 pKa = 9.41KK66 pKa = 9.88KK67 pKa = 8.64RR68 pKa = 11.84MRR70 pKa = 11.84RR71 pKa = 11.84LKK73 pKa = 10.08RR74 pKa = 11.84KK75 pKa = 8.21RR76 pKa = 11.84RR77 pKa = 11.84KK78 pKa = 8.46MRR80 pKa = 11.84QRR82 pKa = 11.84SKK84 pKa = 11.41

Molecular weight: 9.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41937

0

41937

16655602

44

5454

397.2

44.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.742 ± 0.011

1.88 ± 0.006

5.249 ± 0.008

6.413 ± 0.014

4.2 ± 0.008

6.622 ± 0.01

2.434 ± 0.005

5.144 ± 0.009

6.107 ± 0.012

9.748 ± 0.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.425 ± 0.005

4.449 ± 0.01

4.995 ± 0.012

3.721 ± 0.008

5.3 ± 0.009

8.931 ± 0.015

4.956 ± 0.007

6.618 ± 0.008

1.308 ± 0.005

2.757 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski