Amino acid dipepetide frequency for Verrucomicrobia bacterium LW23

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.824AlaAla: 15.824 ± 0.194	1.126AlaCys: 1.126 ± 0.027	5.625AlaAsp: 5.625 ± 0.061	7.126AlaGlu: 7.126 ± 0.095	3.729AlaPhe: 3.729 ± 0.05	9.625AlaGly: 9.625 ± 0.098	2.009AlaHis: 2.009 ± 0.036	5.038AlaIle: 5.038 ± 0.058	3.942AlaLys: 3.942 ± 0.052	11.05AlaLeu: 11.05 ± 0.12	2.873AlaMet: 2.873 ± 0.047	3.053AlaAsn: 3.053 ± 0.038	7.725AlaPro: 7.725 ± 0.101	4.087AlaGln: 4.087 ± 0.065	7.469AlaArg: 7.469 ± 0.084	6.81AlaSer: 6.81 ± 0.068	6.914AlaThr: 6.914 ± 0.086	7.447AlaVal: 7.447 ± 0.069	1.712AlaTrp: 1.712 ± 0.033	2.327AlaTyr: 2.327 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.976CysAla: 0.976 ± 0.027	0.17CysCys: 0.17 ± 0.01	0.518CysAsp: 0.518 ± 0.018	0.538CysGlu: 0.538 ± 0.017	0.431CysPhe: 0.431 ± 0.016	0.933CysGly: 0.933 ± 0.02	0.24CysHis: 0.24 ± 0.012	0.519CysIle: 0.519 ± 0.02	0.296CysLys: 0.296 ± 0.014	0.991CysLeu: 0.991 ± 0.025	0.226CysMet: 0.226 ± 0.011	0.303CysAsn: 0.303 ± 0.012	0.533CysPro: 0.533 ± 0.017	0.283CysGln: 0.283 ± 0.014	0.676CysArg: 0.676 ± 0.021	0.553CysSer: 0.553 ± 0.016	0.532CysThr: 0.532 ± 0.017	0.731CysVal: 0.731 ± 0.022	0.154CysTrp: 0.154 ± 0.009	0.286CysTyr: 0.286 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	5.677AspAla: 5.677 ± 0.065	0.485AspCys: 0.485 ± 0.018	2.626AspAsp: 2.626 ± 0.046	3.081AspGlu: 3.081 ± 0.052	2.182AspPhe: 2.182 ± 0.034	4.323AspGly: 4.323 ± 0.058	0.898AspHis: 0.898 ± 0.026	2.797AspIle: 2.797 ± 0.041	2.244AspLys: 2.244 ± 0.047	4.742AspLeu: 4.742 ± 0.054	1.077AspMet: 1.077 ± 0.02	1.564AspAsn: 1.564 ± 0.031	3.134AspPro: 3.134 ± 0.051	1.391AspGln: 1.391 ± 0.029	2.886AspArg: 2.886 ± 0.038	2.889AspSer: 2.889 ± 0.039	2.736AspThr: 2.736 ± 0.041	3.564AspVal: 3.564 ± 0.049	0.926AspTrp: 0.926 ± 0.024	1.546AspTyr: 1.546 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	6.84GluAla: 6.84 ± 0.106	0.467GluCys: 0.467 ± 0.019	2.596GluAsp: 2.596 ± 0.037	3.563GluGlu: 3.563 ± 0.062	2.106GluPhe: 2.106 ± 0.036	4.07GluGly: 4.07 ± 0.05	1.152GluHis: 1.152 ± 0.03	3.333GluIle: 3.333 ± 0.048	2.948GluLys: 2.948 ± 0.056	5.707GluLeu: 5.707 ± 0.086	1.483GluMet: 1.483 ± 0.034	1.688GluAsn: 1.688 ± 0.037	2.849GluPro: 2.849 ± 0.052	2.004GluGln: 2.004 ± 0.041	3.962GluArg: 3.962 ± 0.059	3.143GluSer: 3.143 ± 0.05	3.091GluThr: 3.091 ± 0.05	3.992GluVal: 3.992 ± 0.056	0.891GluTrp: 0.891 ± 0.024	1.372GluTyr: 1.372 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	3.893PheAla: 3.893 ± 0.053	0.481PheCys: 0.481 ± 0.017	2.065PheAsp: 2.065 ± 0.034	1.892PheGlu: 1.892 ± 0.034	1.506PhePhe: 1.506 ± 0.029	2.881PheGly: 2.881 ± 0.043	0.821PheHis: 0.821 ± 0.022	1.723PheIle: 1.723 ± 0.033	1.056PheLys: 1.056 ± 0.029	3.367PheLeu: 3.367 ± 0.053	0.751PheMet: 0.751 ± 0.02	1.197PheAsn: 1.197 ± 0.028	1.843PhePro: 1.843 ± 0.033	1.113PheGln: 1.113 ± 0.022	2.38PheArg: 2.38 ± 0.037	2.572PheSer: 2.572 ± 0.043	2.445PheThr: 2.445 ± 0.043	2.506PheVal: 2.506 ± 0.038	0.562PheTrp: 0.562 ± 0.02	1.141PheTyr: 1.141 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	8.178GlyAla: 8.178 ± 0.079	0.832GlyCys: 0.832 ± 0.024	4.181GlyAsp: 4.181 ± 0.056	4.422GlyGlu: 4.422 ± 0.049	2.929GlyPhe: 2.929 ± 0.046	7.362GlyGly: 7.362 ± 0.179	1.691GlyHis: 1.691 ± 0.043	4.012GlyIle: 4.012 ± 0.053	4.022GlyLys: 4.022 ± 0.06	6.81GlyLeu: 6.81 ± 0.072	2.01GlyMet: 2.01 ± 0.036	2.909GlyAsn: 2.909 ± 0.07	3.518GlyPro: 3.518 ± 0.051	2.644GlyGln: 2.644 ± 0.049	4.946GlyArg: 4.946 ± 0.053	4.874GlySer: 4.874 ± 0.078	5.305GlyThr: 5.305 ± 0.092	5.417GlyVal: 5.417 ± 0.064	1.322GlyTrp: 1.322 ± 0.03	2.286GlyTyr: 2.286 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	2.188HisAla: 2.188 ± 0.04	0.231HisCys: 0.231 ± 0.012	1.037HisAsp: 1.037 ± 0.032	1.036HisGlu: 1.036 ± 0.027	0.966HisPhe: 0.966 ± 0.026	1.787HisGly: 1.787 ± 0.034	0.601HisHis: 0.601 ± 0.026	1.091HisIle: 1.091 ± 0.027	0.672HisLys: 0.672 ± 0.017	2.112HisLeu: 2.112 ± 0.039	0.459HisMet: 0.459 ± 0.017	0.682HisAsn: 0.682 ± 0.019	1.496HisPro: 1.496 ± 0.035	0.687HisGln: 0.687 ± 0.02	1.384HisArg: 1.384 ± 0.031	1.16HisSer: 1.16 ± 0.026	1.092HisThr: 1.092 ± 0.026	1.483HisVal: 1.483 ± 0.035	0.323HisTrp: 0.323 ± 0.014	0.686HisTyr: 0.686 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	5.799IleAla: 5.799 ± 0.068	0.571IleCys: 0.571 ± 0.018	2.586IleAsp: 2.586 ± 0.048	2.7IleGlu: 2.7 ± 0.042	1.793IlePhe: 1.793 ± 0.036	3.544IleGly: 3.544 ± 0.048	1.115IleHis: 1.115 ± 0.024	2.33IleIle: 2.33 ± 0.045	1.618IleLys: 1.618 ± 0.035	4.444IleLeu: 4.444 ± 0.054	0.904IleMet: 0.904 ± 0.022	1.685IleAsn: 1.685 ± 0.043	2.843IlePro: 2.843 ± 0.044	1.464IleGln: 1.464 ± 0.028	3.299IleArg: 3.299 ± 0.037	3.22IleSer: 3.22 ± 0.048	3.192IleThr: 3.192 ± 0.075	3.528IleVal: 3.528 ± 0.049	0.565IleTrp: 0.565 ± 0.018	1.387IleTyr: 1.387 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	4.502LysAla: 4.502 ± 0.063	0.274LysCys: 0.274 ± 0.013	2.111LysAsp: 2.111 ± 0.037	2.435LysGlu: 2.435 ± 0.051	1.115LysPhe: 1.115 ± 0.026	2.813LysGly: 2.813 ± 0.046	0.718LysHis: 0.718 ± 0.02	1.95LysIle: 1.95 ± 0.036	1.912LysLys: 1.912 ± 0.04	3.749LysLeu: 3.749 ± 0.062	0.934LysMet: 0.934 ± 0.024	1.142LysAsn: 1.142 ± 0.029	2.505LysPro: 2.505 ± 0.046	1.255LysGln: 1.255 ± 0.031	2.189LysArg: 2.189 ± 0.031	2.22LysSer: 2.22 ± 0.04	2.055LysThr: 2.055 ± 0.041	2.634LysVal: 2.634 ± 0.05	0.513LysTrp: 0.513 ± 0.017	0.981LysTyr: 0.981 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	11.264LeuAla: 11.264 ± 0.114	1.122LeuCys: 1.122 ± 0.026	4.933LeuAsp: 4.933 ± 0.061	5.408LeuGlu: 5.408 ± 0.083	3.27LeuPhe: 3.27 ± 0.045	7.03LeuGly: 7.03 ± 0.078	2.199LeuHis: 2.199 ± 0.044	4.293LeuIle: 4.293 ± 0.058	3.665LeuLys: 3.665 ± 0.055	9.371LeuLeu: 9.371 ± 0.118	2.09LeuMet: 2.09 ± 0.039	2.95LeuAsn: 2.95 ± 0.049	6.165LeuPro: 6.165 ± 0.074	3.384LeuGln: 3.384 ± 0.056	7.305LeuArg: 7.305 ± 0.091	6.04LeuSer: 6.04 ± 0.063	5.766LeuThr: 5.766 ± 0.071	6.309LeuVal: 6.309 ± 0.063	1.255LeuTrp: 1.255 ± 0.03	2.371LeuTyr: 2.371 ± 0.039	0.0LeuXaa: 0.0 ± 0.0
Met	2.558MetAla: 2.558 ± 0.038	0.204MetCys: 0.204 ± 0.011	1.151MetAsp: 1.151 ± 0.025	1.414MetGlu: 1.414 ± 0.035	0.643MetPhe: 0.643 ± 0.018	1.625MetGly: 1.625 ± 0.028	0.526MetHis: 0.526 ± 0.016	1.045MetIle: 1.045 ± 0.025	1.009MetLys: 1.009 ± 0.026	2.41MetLeu: 2.41 ± 0.044	0.571MetMet: 0.571 ± 0.019	0.717MetAsn: 0.717 ± 0.021	1.56MetPro: 1.56 ± 0.028	0.903MetGln: 0.903 ± 0.025	1.672MetArg: 1.672 ± 0.034	1.478MetSer: 1.478 ± 0.028	1.34MetThr: 1.34 ± 0.027	1.463MetVal: 1.463 ± 0.032	0.306MetTrp: 0.306 ± 0.014	0.431MetTyr: 0.431 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.501AsnAla: 3.501 ± 0.051	0.276AsnCys: 0.276 ± 0.012	1.328AsnAsp: 1.328 ± 0.03	1.425AsnGlu: 1.425 ± 0.029	1.105AsnPhe: 1.105 ± 0.026	2.702AsnGly: 2.702 ± 0.062	0.573AsnHis: 0.573 ± 0.021	1.691AsnIle: 1.691 ± 0.04	1.087AsnLys: 1.087 ± 0.028	3.031AsnLeu: 3.031 ± 0.042	0.668AsnMet: 0.668 ± 0.02	1.172AsnAsn: 1.172 ± 0.043	2.283AsnPro: 2.283 ± 0.037	0.935AsnGln: 0.935 ± 0.022	1.939AsnArg: 1.939 ± 0.04	1.885AsnSer: 1.885 ± 0.052	1.811AsnThr: 1.811 ± 0.052	2.181AsnVal: 2.181 ± 0.046	0.492AsnTrp: 0.492 ± 0.018	0.923AsnTyr: 0.923 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	8.778ProAla: 8.778 ± 0.129	0.465ProCys: 0.465 ± 0.018	3.465ProAsp: 3.465 ± 0.048	3.906ProGlu: 3.906 ± 0.055	1.991ProPhe: 1.991 ± 0.044	5.841ProGly: 5.841 ± 0.064	1.318ProHis: 1.318 ± 0.03	2.264ProIle: 2.264 ± 0.037	1.764ProLys: 1.764 ± 0.032	5.151ProLeu: 5.151 ± 0.061	1.282ProMet: 1.282 ± 0.026	1.67ProAsn: 1.67 ± 0.037	4.288ProPro: 4.288 ± 0.103	2.124ProGln: 2.124 ± 0.037	3.575ProArg: 3.575 ± 0.04	3.801ProSer: 3.801 ± 0.055	3.285ProThr: 3.285 ± 0.052	4.534ProVal: 4.534 ± 0.056	0.887ProTrp: 0.887 ± 0.02	1.391ProTyr: 1.391 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.722GlnAla: 3.722 ± 0.055	0.287GlnCys: 0.287 ± 0.014	1.47GlnAsp: 1.47 ± 0.031	1.847GlnGlu: 1.847 ± 0.04	1.137GlnPhe: 1.137 ± 0.027	2.538GlnGly: 2.538 ± 0.045	0.787GlnHis: 0.787 ± 0.024	1.794GlnIle: 1.794 ± 0.034	1.381GlnLys: 1.381 ± 0.031	3.335GlnLeu: 3.335 ± 0.058	0.833GlnMet: 0.833 ± 0.021	1.006GlnAsn: 1.006 ± 0.025	2.334GlnPro: 2.334 ± 0.041	1.624GlnGln: 1.624 ± 0.039	2.551GlnArg: 2.551 ± 0.036	2.01GlnSer: 2.01 ± 0.034	1.795GlnThr: 1.795 ± 0.034	2.349GlnVal: 2.349 ± 0.042	0.515GlnTrp: 0.515 ± 0.018	0.869GlnTyr: 0.869 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	6.612ArgAla: 6.612 ± 0.071	0.633ArgCys: 0.633 ± 0.022	3.486ArgAsp: 3.486 ± 0.049	4.307ArgGlu: 4.307 ± 0.067	2.592ArgPhe: 2.592 ± 0.041	4.435ArgGly: 4.435 ± 0.054	1.622ArgHis: 1.622 ± 0.034	3.559ArgIle: 3.559 ± 0.048	2.595ArgLys: 2.595 ± 0.038	6.716ArgLeu: 6.716 ± 0.072	1.751ArgMet: 1.751 ± 0.032	2.237ArgAsn: 2.237 ± 0.04	3.688ArgPro: 3.688 ± 0.048	2.52ArgGln: 2.52 ± 0.043	4.929ArgArg: 4.929 ± 0.075	3.494ArgSer: 3.494 ± 0.047	3.606ArgThr: 3.606 ± 0.051	4.592ArgVal: 4.592 ± 0.049	1.021ArgTrp: 1.021 ± 0.028	1.957ArgTyr: 1.957 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	6.893SerAla: 6.893 ± 0.078	0.525SerCys: 0.525 ± 0.017	2.895SerAsp: 2.895 ± 0.039	2.881SerGlu: 2.881 ± 0.044	2.319SerPhe: 2.319 ± 0.04	5.502SerGly: 5.502 ± 0.084	1.306SerHis: 1.306 ± 0.026	2.917SerIle: 2.917 ± 0.043	2.003SerLys: 2.003 ± 0.045	5.868SerLeu: 5.868 ± 0.062	1.348SerMet: 1.348 ± 0.025	1.778SerAsn: 1.778 ± 0.041	4.191SerPro: 4.191 ± 0.057	1.997SerGln: 1.997 ± 0.04	3.808SerArg: 3.808 ± 0.048	4.081SerSer: 4.081 ± 0.059	3.855SerThr: 3.855 ± 0.067	4.032SerVal: 4.032 ± 0.05	0.828SerTrp: 0.828 ± 0.02	1.556SerTyr: 1.556 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	6.737ThrAla: 6.737 ± 0.081	0.531ThrCys: 0.531 ± 0.016	2.727ThrAsp: 2.727 ± 0.047	2.941ThrGlu: 2.941 ± 0.043	2.284ThrPhe: 2.284 ± 0.047	5.2ThrGly: 5.2 ± 0.081	1.226ThrHis: 1.226 ± 0.024	2.891ThrIle: 2.891 ± 0.065	1.679ThrLys: 1.679 ± 0.034	6.145ThrLeu: 6.145 ± 0.085	1.231ThrMet: 1.231 ± 0.027	1.694ThrAsn: 1.694 ± 0.047	4.494ThrPro: 4.494 ± 0.06	1.865ThrGln: 1.865 ± 0.036	3.548ThrArg: 3.548 ± 0.045	3.867ThrSer: 3.867 ± 0.062	3.729ThrThr: 3.729 ± 0.074	4.12ThrVal: 4.12 ± 0.057	0.955ThrTrp: 0.955 ± 0.025	1.725ThrTyr: 1.725 ± 0.049	0.0ThrXaa: 0.0 ± 0.0
Val	7.853ValAla: 7.853 ± 0.084	0.722ValCys: 0.722 ± 0.022	3.633ValAsp: 3.633 ± 0.058	4.202ValGlu: 4.202 ± 0.052	2.335ValPhe: 2.335 ± 0.04	4.406ValGly: 4.406 ± 0.059	1.409ValHis: 1.409 ± 0.03	3.374ValIle: 3.374 ± 0.049	2.45ValLys: 2.45 ± 0.049	6.978ValLeu: 6.978 ± 0.076	1.547ValMet: 1.547 ± 0.032	2.1ValAsn: 2.1 ± 0.043	4.228ValPro: 4.228 ± 0.05	2.303ValGln: 2.303 ± 0.038	4.787ValArg: 4.787 ± 0.062	4.057ValSer: 4.057 ± 0.053	4.522ValThr: 4.522 ± 0.07	5.375ValVal: 5.375 ± 0.064	0.983ValTrp: 0.983 ± 0.022	1.747ValTyr: 1.747 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.229TrpAla: 1.229 ± 0.029	0.183TrpCys: 0.183 ± 0.01	0.8TrpAsp: 0.8 ± 0.025	0.737TrpGlu: 0.737 ± 0.022	0.573TrpPhe: 0.573 ± 0.019	0.95TrpGly: 0.95 ± 0.025	0.397TrpHis: 0.397 ± 0.017	0.761TrpIle: 0.761 ± 0.024	0.707TrpLys: 0.707 ± 0.02	1.689TrpLeu: 1.689 ± 0.035	0.447TrpMet: 0.447 ± 0.016	0.543TrpAsn: 0.543 ± 0.016	0.738TrpPro: 0.738 ± 0.023	0.672TrpGln: 0.672 ± 0.021	1.167TrpArg: 1.167 ± 0.03	0.848TrpSer: 0.848 ± 0.021	0.878TrpThr: 0.878 ± 0.025	0.921TrpVal: 0.921 ± 0.024	0.286TrpTrp: 0.286 ± 0.012	0.402TrpTyr: 0.402 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.49TyrAla: 2.49 ± 0.046	0.305TyrCys: 0.305 ± 0.014	1.535TyrAsp: 1.535 ± 0.045	1.396TyrGlu: 1.396 ± 0.03	1.189TyrPhe: 1.189 ± 0.029	2.104TyrGly: 2.104 ± 0.043	0.565TyrHis: 0.565 ± 0.017	1.208TyrIle: 1.208 ± 0.029	0.957TyrLys: 0.957 ± 0.027	2.523TyrLeu: 2.523 ± 0.038	0.538TyrMet: 0.538 ± 0.021	0.917TyrAsn: 0.917 ± 0.027	1.365TyrPro: 1.365 ± 0.031	0.927TyrGln: 0.927 ± 0.024	1.88TyrArg: 1.88 ± 0.034	1.582TyrSer: 1.582 ± 0.032	1.64TyrThr: 1.64 ± 0.04	1.832TyrVal: 1.832 ± 0.037	0.432TyrTrp: 0.432 ± 0.016	0.906TyrTyr: 0.906 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4752 proteins (1799784 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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