Microbacterium telephonicum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2897 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A498BUL3|A0A498BUL3_9MICO Pyruvate formate lyase activating enzyme OS=Microbacterium telephonicum OX=1714841 GN=C7474_2153 PE=3 SV=1
MM1 pKa = 7.65AAQQRR6 pKa = 11.84RR7 pKa = 11.84HH8 pKa = 4.0VTRR11 pKa = 11.84TLGAGIALIGLIALTACSGGGGEE34 pKa = 4.37VNSDD38 pKa = 3.48YY39 pKa = 11.64GFATAEE45 pKa = 3.94QTADD49 pKa = 3.08SAITVWVDD57 pKa = 3.03AARR60 pKa = 11.84EE61 pKa = 4.07PIAKK65 pKa = 10.02AFEE68 pKa = 4.53ADD70 pKa = 3.69NPDD73 pKa = 2.99VKK75 pKa = 10.97INIEE79 pKa = 4.32TYY81 pKa = 10.34DD82 pKa = 3.71GNAGGSGSFQTKK94 pKa = 9.16VALFDD99 pKa = 3.52QSGEE103 pKa = 3.85GWPDD107 pKa = 3.26VVFSTQTNDD116 pKa = 3.07ASWAAKK122 pKa = 7.14EE123 pKa = 4.38TNGVQAFAAPLDD135 pKa = 3.99KK136 pKa = 11.3GFLDD140 pKa = 4.34QDD142 pKa = 3.78FLDD145 pKa = 4.86GFTSGANDD153 pKa = 3.57PMTVDD158 pKa = 3.56GSVYY162 pKa = 10.81GLRR165 pKa = 11.84NDD167 pKa = 4.41LAPVVLWYY175 pKa = 9.9NQKK178 pKa = 10.66LLDD181 pKa = 3.92EE182 pKa = 5.17FGYY185 pKa = 10.29DD186 pKa = 4.98IPQTWEE192 pKa = 4.09DD193 pKa = 3.79YY194 pKa = 10.6QALGDD199 pKa = 4.06KK200 pKa = 10.29LAAEE204 pKa = 4.41HH205 pKa = 6.84PGYY208 pKa = 10.44ILGSVGDD215 pKa = 4.43SFVGTYY221 pKa = 9.65VYY223 pKa = 11.01YY224 pKa = 10.08WGAQAPIFQLDD235 pKa = 3.57GNTFSSDD242 pKa = 3.12FDD244 pKa = 4.6DD245 pKa = 3.8EE246 pKa = 5.17HH247 pKa = 8.24SVAMTDD253 pKa = 3.56MLDD256 pKa = 3.39HH257 pKa = 6.1MVGNGTLVQDD267 pKa = 4.24SVFSAGFVEE276 pKa = 5.3NYY278 pKa = 9.71ADD280 pKa = 5.27KK281 pKa = 10.47IVAMPGPAWYY291 pKa = 10.33SGALFQNPDD300 pKa = 3.67SLNIPAGEE308 pKa = 4.21IGAADD313 pKa = 4.0PLYY316 pKa = 10.24WDD318 pKa = 4.37GQDD321 pKa = 3.12EE322 pKa = 4.66VTGNVGGGVWYY333 pKa = 10.34ASSHH337 pKa = 5.13SANLEE342 pKa = 3.85AVKK345 pKa = 9.93TFLEE349 pKa = 4.32YY350 pKa = 10.85VISSEE355 pKa = 4.33KK356 pKa = 10.57AVEE359 pKa = 4.03LASGLPAYY367 pKa = 10.37ADD369 pKa = 4.39AASQWLDD376 pKa = 3.41TQASSGYY383 pKa = 10.11YY384 pKa = 10.57DD385 pKa = 4.31GDD387 pKa = 3.4FKK389 pKa = 11.6AAVEE393 pKa = 4.13KK394 pKa = 10.71AAGSVWSGWGFPSFSPEE411 pKa = 3.26TAYY414 pKa = 10.73AAVVVPGLAAGKK426 pKa = 10.07SIADD430 pKa = 3.93VVPDD434 pKa = 3.13WHH436 pKa = 5.75TQILNEE442 pKa = 4.12AQVQGYY448 pKa = 6.84EE449 pKa = 3.98TAEE452 pKa = 3.88

Molecular weight: 47.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A498CMH4|A0A498CMH4_9MICO Carbohydrate ABC transporter substrate-binding protein (CUT1 family) OS=Microbacterium telephonicum OX=1714841 GN=C7474_0870 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2897

0

2897

950115

32

2021

328.0

34.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.359 ± 0.069

0.472 ± 0.009

6.532 ± 0.043

5.305 ± 0.039

3.06 ± 0.028

9.006 ± 0.039

1.972 ± 0.021

4.314 ± 0.034

1.818 ± 0.034

9.978 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.703 ± 0.017

1.775 ± 0.027

5.494 ± 0.031

2.716 ± 0.024

7.413 ± 0.055

5.151 ± 0.03

6.176 ± 0.035

9.252 ± 0.047

1.545 ± 0.022

1.96 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski