Amino acid dipepetide frequency for Gordonia phage Soups

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.705AlaAla: 9.705 ± 1.028
0.742AlaCys: 0.742 ± 0.196
5.378AlaAsp: 5.378 ± 0.637
7.356AlaGlu: 7.356 ± 0.823
3.833AlaPhe: 3.833 ± 0.629
7.665AlaGly: 7.665 ± 0.867
1.175AlaHis: 1.175 ± 0.221
4.513AlaIle: 4.513 ± 0.446
4.636AlaLys: 4.636 ± 0.62
6.676AlaLeu: 6.676 ± 0.676
2.411AlaMet: 2.411 ± 0.316
1.978AlaAsn: 1.978 ± 0.413
4.698AlaPro: 4.698 ± 0.67
3.647AlaGln: 3.647 ± 0.407
5.254AlaArg: 5.254 ± 0.594
5.131AlaSer: 5.131 ± 0.533
5.811AlaThr: 5.811 ± 0.653
6.923AlaVal: 6.923 ± 0.619
2.04AlaTrp: 2.04 ± 0.358
2.164AlaTyr: 2.164 ± 0.295
0.0AlaXaa: 0.0 ± 0.0
Cys
0.742CysAla: 0.742 ± 0.276
0.185CysCys: 0.185 ± 0.162
0.804CysAsp: 0.804 ± 0.239
0.247CysGlu: 0.247 ± 0.109
0.247CysPhe: 0.247 ± 0.148
0.68CysGly: 0.68 ± 0.248
0.247CysHis: 0.247 ± 0.134
0.309CysIle: 0.309 ± 0.126
0.433CysLys: 0.433 ± 0.155
0.804CysLeu: 0.804 ± 0.233
0.309CysMet: 0.309 ± 0.145
0.495CysAsn: 0.495 ± 0.159
0.556CysPro: 0.556 ± 0.216
0.185CysGln: 0.185 ± 0.164
0.433CysArg: 0.433 ± 0.181
0.742CysSer: 0.742 ± 0.204
0.247CysThr: 0.247 ± 0.105
0.371CysVal: 0.371 ± 0.157
0.247CysTrp: 0.247 ± 0.105
0.309CysTyr: 0.309 ± 0.139
0.0CysXaa: 0.0 ± 0.0
Asp
5.316AspAla: 5.316 ± 0.682
0.618AspCys: 0.618 ± 0.241
5.502AspAsp: 5.502 ± 0.831
4.513AspGlu: 4.513 ± 0.587
2.411AspPhe: 2.411 ± 0.329
5.687AspGly: 5.687 ± 0.609
1.545AspHis: 1.545 ± 0.395
2.967AspIle: 2.967 ± 0.5
2.287AspLys: 2.287 ± 0.44
6.367AspLeu: 6.367 ± 0.645
1.051AspMet: 1.051 ± 0.237
2.164AspAsn: 2.164 ± 0.406
5.131AspPro: 5.131 ± 0.509
2.225AspGln: 2.225 ± 0.375
3.338AspArg: 3.338 ± 0.464
3.4AspSer: 3.4 ± 0.455
4.08AspThr: 4.08 ± 0.511
4.018AspVal: 4.018 ± 0.481
1.36AspTrp: 1.36 ± 0.307
2.72AspTyr: 2.72 ± 0.449
0.0AspXaa: 0.0 ± 0.0
Glu
8.531GluAla: 8.531 ± 0.746
0.124GluCys: 0.124 ± 0.08
5.44GluAsp: 5.44 ± 0.596
4.018GluGlu: 4.018 ± 0.648
2.411GluPhe: 2.411 ± 0.353
5.316GluGly: 5.316 ± 0.596
1.916GluHis: 1.916 ± 0.394
4.142GluIle: 4.142 ± 0.561
2.905GluLys: 2.905 ± 0.534
6.614GluLeu: 6.614 ± 0.526
1.731GluMet: 1.731 ± 0.33
1.793GluAsn: 1.793 ± 0.332
2.473GluPro: 2.473 ± 0.412
2.164GluGln: 2.164 ± 0.352
3.833GluArg: 3.833 ± 0.508
3.214GluSer: 3.214 ± 0.398
4.513GluThr: 4.513 ± 0.623
5.502GluVal: 5.502 ± 0.656
1.36GluTrp: 1.36 ± 0.259
1.854GluTyr: 1.854 ± 0.361
0.0GluXaa: 0.0 ± 0.0
Phe
3.338PheAla: 3.338 ± 0.495
0.247PheCys: 0.247 ± 0.146
2.967PheAsp: 2.967 ± 0.47
3.153PheGlu: 3.153 ± 0.499
1.113PhePhe: 1.113 ± 0.259
3.524PheGly: 3.524 ± 0.476
0.989PheHis: 0.989 ± 0.274
1.236PheIle: 1.236 ± 0.257
1.484PheLys: 1.484 ± 0.279
2.102PheLeu: 2.102 ± 0.347
0.927PheMet: 0.927 ± 0.212
1.669PheAsn: 1.669 ± 0.321
1.731PhePro: 1.731 ± 0.313
0.865PheGln: 0.865 ± 0.206
1.422PheArg: 1.422 ± 0.244
2.411PheSer: 2.411 ± 0.461
2.102PheThr: 2.102 ± 0.392
2.411PheVal: 2.411 ± 0.366
0.309PheTrp: 0.309 ± 0.122
0.804PheTyr: 0.804 ± 0.248
0.0PheXaa: 0.0 ± 0.0
Gly
6.12GlyAla: 6.12 ± 0.632
0.989GlyCys: 0.989 ± 0.267
5.625GlyAsp: 5.625 ± 0.624
5.378GlyGlu: 5.378 ± 0.597
3.153GlyPhe: 3.153 ± 0.514
7.912GlyGly: 7.912 ± 1.508
2.164GlyHis: 2.164 ± 0.369
3.894GlyIle: 3.894 ± 0.651
3.709GlyLys: 3.709 ± 0.543
6.367GlyLeu: 6.367 ± 0.774
1.731GlyMet: 1.731 ± 0.298
2.782GlyAsn: 2.782 ± 0.35
3.338GlyPro: 3.338 ± 0.521
2.411GlyGln: 2.411 ± 0.455
4.636GlyArg: 4.636 ± 0.471
4.636GlySer: 4.636 ± 0.648
5.563GlyThr: 5.563 ± 0.539
5.131GlyVal: 5.131 ± 0.596
2.164GlyTrp: 2.164 ± 0.369
3.214GlyTyr: 3.214 ± 0.466
0.0GlyXaa: 0.0 ± 0.0
His
1.793HisAla: 1.793 ± 0.325
0.309HisCys: 0.309 ± 0.125
1.051HisAsp: 1.051 ± 0.205
1.422HisGlu: 1.422 ± 0.352
0.742HisPhe: 0.742 ± 0.217
1.669HisGly: 1.669 ± 0.337
0.433HisHis: 0.433 ± 0.18
1.298HisIle: 1.298 ± 0.261
0.556HisLys: 0.556 ± 0.199
2.164HisLeu: 2.164 ± 0.397
0.247HisMet: 0.247 ± 0.122
0.927HisAsn: 0.927 ± 0.269
0.865HisPro: 0.865 ± 0.206
0.68HisGln: 0.68 ± 0.211
1.793HisArg: 1.793 ± 0.293
0.989HisSer: 0.989 ± 0.287
1.113HisThr: 1.113 ± 0.279
1.113HisVal: 1.113 ± 0.26
0.309HisTrp: 0.309 ± 0.166
0.865HisTyr: 0.865 ± 0.275
0.0HisXaa: 0.0 ± 0.0
Ile
4.389IleAla: 4.389 ± 0.539
0.556IleCys: 0.556 ± 0.168
3.709IleAsp: 3.709 ± 0.481
4.574IleGlu: 4.574 ± 0.595
1.607IlePhe: 1.607 ± 0.218
3.709IleGly: 3.709 ± 0.601
1.175IleHis: 1.175 ± 0.27
2.349IleIle: 2.349 ± 0.407
1.731IleLys: 1.731 ± 0.291
3.894IleLeu: 3.894 ± 0.428
0.927IleMet: 0.927 ± 0.272
2.102IleAsn: 2.102 ± 0.384
3.462IlePro: 3.462 ± 0.364
1.422IleGln: 1.422 ± 0.253
3.276IleArg: 3.276 ± 0.384
3.214IleSer: 3.214 ± 0.377
3.833IleThr: 3.833 ± 0.435
3.462IleVal: 3.462 ± 0.457
0.742IleTrp: 0.742 ± 0.193
1.113IleTyr: 1.113 ± 0.233
0.0IleXaa: 0.0 ± 0.0
Lys
5.378LysAla: 5.378 ± 0.712
0.185LysCys: 0.185 ± 0.139
2.287LysAsp: 2.287 ± 0.293
3.091LysGlu: 3.091 ± 0.407
1.236LysPhe: 1.236 ± 0.271
3.338LysGly: 3.338 ± 0.406
0.68LysHis: 0.68 ± 0.169
2.287LysIle: 2.287 ± 0.449
3.276LysLys: 3.276 ± 0.601
4.636LysLeu: 4.636 ± 0.544
1.051LysMet: 1.051 ± 0.244
1.051LysAsn: 1.051 ± 0.251
2.905LysPro: 2.905 ± 0.473
1.978LysGln: 1.978 ± 0.319
2.473LysArg: 2.473 ± 0.4
2.102LysSer: 2.102 ± 0.406
2.102LysThr: 2.102 ± 0.399
3.956LysVal: 3.956 ± 0.556
0.865LysTrp: 0.865 ± 0.205
1.175LysTyr: 1.175 ± 0.284
0.0LysXaa: 0.0 ± 0.0
Leu
7.356LeuAla: 7.356 ± 0.596
0.556LeuCys: 0.556 ± 0.169
5.625LeuAsp: 5.625 ± 0.688
6.923LeuGlu: 6.923 ± 0.578
2.72LeuPhe: 2.72 ± 0.455
6.429LeuGly: 6.429 ± 0.688
1.422LeuHis: 1.422 ± 0.317
4.513LeuIle: 4.513 ± 0.648
3.091LeuLys: 3.091 ± 0.597
5.254LeuLeu: 5.254 ± 0.64
2.349LeuMet: 2.349 ± 0.474
2.596LeuAsn: 2.596 ± 0.37
4.203LeuPro: 4.203 ± 0.503
2.102LeuGln: 2.102 ± 0.291
5.316LeuArg: 5.316 ± 0.486
4.76LeuSer: 4.76 ± 0.644
4.76LeuThr: 4.76 ± 0.544
6.553LeuVal: 6.553 ± 0.672
1.731LeuTrp: 1.731 ± 0.313
2.534LeuTyr: 2.534 ± 0.438
0.0LeuXaa: 0.0 ± 0.0
Met
2.596MetAla: 2.596 ± 0.376
0.185MetCys: 0.185 ± 0.121
0.989MetAsp: 0.989 ± 0.268
1.298MetGlu: 1.298 ± 0.248
1.298MetPhe: 1.298 ± 0.302
1.36MetGly: 1.36 ± 0.264
0.309MetHis: 0.309 ± 0.156
1.36MetIle: 1.36 ± 0.313
1.051MetLys: 1.051 ± 0.276
1.669MetLeu: 1.669 ± 0.303
0.742MetMet: 0.742 ± 0.196
0.618MetAsn: 0.618 ± 0.234
1.607MetPro: 1.607 ± 0.337
0.742MetGln: 0.742 ± 0.246
1.854MetArg: 1.854 ± 0.302
2.349MetSer: 2.349 ± 0.37
2.905MetThr: 2.905 ± 0.387
1.051MetVal: 1.051 ± 0.263
0.185MetTrp: 0.185 ± 0.098
0.68MetTyr: 0.68 ± 0.239
0.0MetXaa: 0.0 ± 0.0
Asn
3.091AsnAla: 3.091 ± 0.585
0.495AsnCys: 0.495 ± 0.218
1.793AsnAsp: 1.793 ± 0.305
1.793AsnGlu: 1.793 ± 0.329
0.865AsnPhe: 0.865 ± 0.213
3.709AsnGly: 3.709 ± 0.49
0.804AsnHis: 0.804 ± 0.216
1.422AsnIle: 1.422 ± 0.366
1.545AsnLys: 1.545 ± 0.375
3.029AsnLeu: 3.029 ± 0.43
0.68AsnMet: 0.68 ± 0.183
0.865AsnAsn: 0.865 ± 0.211
2.473AsnPro: 2.473 ± 0.327
1.422AsnGln: 1.422 ± 0.292
1.854AsnArg: 1.854 ± 0.335
1.978AsnSer: 1.978 ± 0.281
2.658AsnThr: 2.658 ± 0.415
1.607AsnVal: 1.607 ± 0.289
0.618AsnTrp: 0.618 ± 0.175
1.36AsnTyr: 1.36 ± 0.253
0.0AsnXaa: 0.0 ± 0.0
Pro
4.327ProAla: 4.327 ± 0.628
0.309ProCys: 0.309 ± 0.17
3.894ProAsp: 3.894 ± 0.465
4.574ProGlu: 4.574 ± 0.468
1.854ProPhe: 1.854 ± 0.333
4.574ProGly: 4.574 ± 0.602
0.742ProHis: 0.742 ± 0.203
2.967ProIle: 2.967 ± 0.447
2.967ProLys: 2.967 ± 0.432
2.72ProLeu: 2.72 ± 0.4
1.545ProMet: 1.545 ± 0.33
1.978ProAsn: 1.978 ± 0.386
2.102ProPro: 2.102 ± 0.397
1.545ProGln: 1.545 ± 0.343
3.029ProArg: 3.029 ± 0.457
2.905ProSer: 2.905 ± 0.406
4.265ProThr: 4.265 ± 0.515
3.585ProVal: 3.585 ± 0.453
0.68ProTrp: 0.68 ± 0.181
1.607ProTyr: 1.607 ± 0.34
0.0ProXaa: 0.0 ± 0.0
Gln
3.709GlnAla: 3.709 ± 0.508
0.124GlnCys: 0.124 ± 0.087
1.545GlnAsp: 1.545 ± 0.338
2.287GlnGlu: 2.287 ± 0.377
1.175GlnPhe: 1.175 ± 0.28
2.04GlnGly: 2.04 ± 0.321
0.618GlnHis: 0.618 ± 0.199
1.793GlnIle: 1.793 ± 0.271
1.36GlnLys: 1.36 ± 0.305
3.709GlnLeu: 3.709 ± 0.486
1.113GlnMet: 1.113 ± 0.278
1.175GlnAsn: 1.175 ± 0.285
1.236GlnPro: 1.236 ± 0.287
0.927GlnGln: 0.927 ± 0.24
2.225GlnArg: 2.225 ± 0.356
1.669GlnSer: 1.669 ± 0.271
2.04GlnThr: 2.04 ± 0.376
2.349GlnVal: 2.349 ± 0.421
0.865GlnTrp: 0.865 ± 0.188
1.298GlnTyr: 1.298 ± 0.247
0.0GlnXaa: 0.0 ± 0.0
Arg
4.822ArgAla: 4.822 ± 0.458
0.618ArgCys: 0.618 ± 0.274
3.585ArgAsp: 3.585 ± 0.454
3.585ArgGlu: 3.585 ± 0.599
2.349ArgPhe: 2.349 ± 0.442
3.956ArgGly: 3.956 ± 0.472
1.607ArgHis: 1.607 ± 0.314
3.956ArgIle: 3.956 ± 0.458
2.905ArgLys: 2.905 ± 0.385
4.822ArgLeu: 4.822 ± 0.62
2.04ArgMet: 2.04 ± 0.299
2.225ArgAsn: 2.225 ± 0.385
2.473ArgPro: 2.473 ± 0.358
1.854ArgGln: 1.854 ± 0.354
4.945ArgArg: 4.945 ± 0.63
3.524ArgSer: 3.524 ± 0.5
3.029ArgThr: 3.029 ± 0.51
4.08ArgVal: 4.08 ± 0.44
0.989ArgTrp: 0.989 ± 0.241
2.102ArgTyr: 2.102 ± 0.419
0.0ArgXaa: 0.0 ± 0.0
Ser
5.193SerAla: 5.193 ± 0.56
0.371SerCys: 0.371 ± 0.187
2.967SerAsp: 2.967 ± 0.439
3.585SerGlu: 3.585 ± 0.442
2.04SerPhe: 2.04 ± 0.346
5.44SerGly: 5.44 ± 0.622
1.051SerHis: 1.051 ± 0.252
2.658SerIle: 2.658 ± 0.418
2.534SerLys: 2.534 ± 0.431
5.378SerLeu: 5.378 ± 0.821
1.978SerMet: 1.978 ± 0.314
2.72SerAsn: 2.72 ± 0.515
2.164SerPro: 2.164 ± 0.325
2.411SerGln: 2.411 ± 0.316
3.462SerArg: 3.462 ± 0.378
3.771SerSer: 3.771 ± 0.635
3.833SerThr: 3.833 ± 0.584
4.265SerVal: 4.265 ± 0.606
1.236SerTrp: 1.236 ± 0.261
1.484SerTyr: 1.484 ± 0.25
0.0SerXaa: 0.0 ± 0.0
Thr
5.749ThrAla: 5.749 ± 0.544
0.804ThrCys: 0.804 ± 0.235
4.574ThrAsp: 4.574 ± 0.456
3.709ThrGlu: 3.709 ± 0.476
1.793ThrPhe: 1.793 ± 0.339
5.378ThrGly: 5.378 ± 0.587
0.927ThrHis: 0.927 ± 0.258
3.524ThrIle: 3.524 ± 0.465
3.956ThrLys: 3.956 ± 0.612
5.069ThrLeu: 5.069 ± 0.559
1.298ThrMet: 1.298 ± 0.299
1.793ThrAsn: 1.793 ± 0.317
4.389ThrPro: 4.389 ± 0.479
2.349ThrGln: 2.349 ± 0.415
2.72ThrArg: 2.72 ± 0.462
4.08ThrSer: 4.08 ± 0.531
4.018ThrThr: 4.018 ± 0.476
5.44ThrVal: 5.44 ± 0.553
1.607ThrTrp: 1.607 ± 0.335
2.658ThrTyr: 2.658 ± 0.353
0.0ThrXaa: 0.0 ± 0.0
Val
5.625ValAla: 5.625 ± 0.667
0.804ValCys: 0.804 ± 0.225
5.193ValAsp: 5.193 ± 0.656
4.945ValGlu: 4.945 ± 0.535
2.225ValPhe: 2.225 ± 0.488
4.76ValGly: 4.76 ± 0.446
1.298ValHis: 1.298 ± 0.262
3.709ValIle: 3.709 ± 0.637
3.771ValLys: 3.771 ± 0.526
5.44ValLeu: 5.44 ± 0.635
1.051ValMet: 1.051 ± 0.27
2.72ValAsn: 2.72 ± 0.384
4.265ValPro: 4.265 ± 0.587
2.287ValGln: 2.287 ± 0.394
4.389ValArg: 4.389 ± 0.485
4.203ValSer: 4.203 ± 0.614
5.007ValThr: 5.007 ± 0.614
4.327ValVal: 4.327 ± 0.554
1.236ValTrp: 1.236 ± 0.261
2.349ValTyr: 2.349 ± 0.359
0.0ValXaa: 0.0 ± 0.0
Trp
1.669TrpAla: 1.669 ± 0.376
0.371TrpCys: 0.371 ± 0.157
1.545TrpAsp: 1.545 ± 0.304
1.607TrpGlu: 1.607 ± 0.309
0.804TrpPhe: 0.804 ± 0.214
1.175TrpGly: 1.175 ± 0.293
0.495TrpHis: 0.495 ± 0.154
1.113TrpIle: 1.113 ± 0.262
0.556TrpLys: 0.556 ± 0.176
1.113TrpLeu: 1.113 ± 0.206
0.309TrpMet: 0.309 ± 0.122
0.989TrpAsn: 0.989 ± 0.253
0.742TrpPro: 0.742 ± 0.243
0.989TrpGln: 0.989 ± 0.222
1.113TrpArg: 1.113 ± 0.261
1.36TrpSer: 1.36 ± 0.308
1.36TrpThr: 1.36 ± 0.259
1.298TrpVal: 1.298 ± 0.271
0.68TrpTrp: 0.68 ± 0.257
0.371TrpTyr: 0.371 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.287TyrAla: 2.287 ± 0.368
0.0TyrCys: 0.0 ± 0.0
2.225TyrAsp: 2.225 ± 0.41
1.793TyrGlu: 1.793 ± 0.357
0.927TyrPhe: 0.927 ± 0.304
2.658TyrGly: 2.658 ± 0.346
0.742TyrHis: 0.742 ± 0.204
1.236TyrIle: 1.236 ± 0.314
1.298TyrLys: 1.298 ± 0.304
2.967TyrLeu: 2.967 ± 0.375
1.298TyrMet: 1.298 ± 0.323
1.422TyrAsn: 1.422 ± 0.355
1.36TyrPro: 1.36 ± 0.267
1.051TyrGln: 1.051 ± 0.239
2.102TyrArg: 2.102 ± 0.383
2.102TyrSer: 2.102 ± 0.436
2.596TyrThr: 2.596 ± 0.427
2.164TyrVal: 2.164 ± 0.307
0.433TyrTrp: 0.433 ± 0.176
0.865TyrTyr: 0.865 ± 0.231
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 99 proteins (16178 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski