Marinitoga piezophila (strain DSM 14283 / JCM 11233 / KA3)

Taxonomy: cellular organisms; Bacteria; Thermotogae; Thermotogae; Petrotogales; Petrotogaceae; Marinitoga; Marinitoga piezophila

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2044 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H2J656|H2J656_MARPK Putative phosphohydrolase OS=Marinitoga piezophila (strain DSM 14283 / JCM 11233 / KA3) OX=443254 GN=Marpi_0679 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 9.01KK3 pKa = 8.18TLVFLGILILALSLTSCLGPAKK25 pKa = 10.68VNATVKK31 pKa = 10.56INWDD35 pKa = 3.28DD36 pKa = 3.31RR37 pKa = 11.84SYY39 pKa = 10.56YY40 pKa = 10.2TDD42 pKa = 3.68DD43 pKa = 3.11STYY46 pKa = 9.43YY47 pKa = 9.88LAYY50 pKa = 10.5VVGTFSTTLDD60 pKa = 4.17NIPKK64 pKa = 10.38EE65 pKa = 4.12GVTNLKK71 pKa = 10.28EE72 pKa = 3.91ITPDD76 pKa = 2.97EE77 pKa = 4.32SEE79 pKa = 4.16YY80 pKa = 10.84SIEE83 pKa = 4.11VSVPAGGNATVFVYY97 pKa = 10.26QDD99 pKa = 2.92KK100 pKa = 11.51DD101 pKa = 3.27GDD103 pKa = 3.93KK104 pKa = 10.6KK105 pKa = 11.49YY106 pKa = 11.14SDD108 pKa = 4.95DD109 pKa = 3.9DD110 pKa = 3.67QAFDD114 pKa = 4.47SEE116 pKa = 4.85WVNPDD121 pKa = 3.5DD122 pKa = 4.42TSIDD126 pKa = 3.58LDD128 pKa = 3.64VYY130 pKa = 10.36YY131 pKa = 11.17

Molecular weight: 14.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2J6X3|H2J6X3_MARPK HD-GYP domain-containing protein OS=Marinitoga piezophila (strain DSM 14283 / JCM 11233 / KA3) OX=443254 GN=Marpi_0814 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.16QPSRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.79RR12 pKa = 11.84KK13 pKa = 7.52NTHH16 pKa = 5.09GFLVRR21 pKa = 11.84SRR23 pKa = 11.84TPGGRR28 pKa = 11.84AVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2044

0

2044

684271

32

1719

334.8

38.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.038 ± 0.055

0.459 ± 0.015

5.278 ± 0.04

8.202 ± 0.065

5.37 ± 0.052

5.882 ± 0.05

1.38 ± 0.019

10.466 ± 0.06

9.934 ± 0.062

9.282 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.024

6.163 ± 0.062

3.153 ± 0.032

1.98 ± 0.025

3.177 ± 0.031

5.658 ± 0.044

4.648 ± 0.036

5.892 ± 0.041

0.81 ± 0.018

4.834 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski